FastQCFastQC Report
Thu 26 May 2016
SRR1513150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675417
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC34120.2036507926086461No Hit
GTACATGGGAAGCAGTGGTATCAAC34100.20353141934216973No Hit
GTCCTACAGTGGACATTTCTAAATT21710.12957968076007348No Hit
GTCCTAAAGTGTGTATTTCTCATTT21610.12898281442769172No Hit
GTATCAACGCAGAGTACTTTTTTTT21390.12766970849645193No Hit
CTTTAGGACGTGAAATATGGCGAGG21040.1255806763331159No Hit
CTGTAGGACGTGGAATATGGCAAGA20420.12188010507234914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT1400.014.96038156
GCGCCAC1059.094947E-1114.46199813
CGTGCGC1006.712071E-1014.23305510
GGCGTGC1450.013.0890398
GCGTGCG1102.7957867E-912.939149
AGGCGTG1550.012.8598847
TTAGTAC604.0164398E-412.69479753
TAGGACC4600.012.4188234
AAAGGCG1700.012.3214215
GTGCGCC1251.4351826E-912.14662811
GGCGAGG6650.011.8426919
GTATTAA1950.011.2337131
TGCGCCA1451.2369128E-911.12701112
TCGAACC600.005928247211.0701549
CGTACTA1158.95574E-710.7260419
GGACGTG12850.010.5945126
AGGACGT13050.010.5789985
AATGTCC3900.010.462848
TAGGACT3200.010.4137014
GTCCTAC12000.010.397291
Produced by FastQC (version 0.11.5)