##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513150_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675417 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.80553020531605 31.0 31.0 34.0 27.0 34.0 2 30.898667615286225 31.0 31.0 34.0 27.0 34.0 3 31.0069212619903 33.0 31.0 34.0 27.0 34.0 4 34.41828631319844 37.0 35.0 37.0 31.0 37.0 5 34.19154514965528 37.0 35.0 37.0 30.0 37.0 6 34.12068219434326 37.0 35.0 37.0 30.0 37.0 7 33.97553265843668 37.0 35.0 37.0 30.0 37.0 8 33.9438665120385 37.0 35.0 37.0 30.0 37.0 9 35.49707445967183 39.0 35.0 39.0 30.0 39.0 10 35.3265945134853 39.0 35.0 39.0 29.0 39.0 11 35.364923478751855 39.0 35.0 39.0 29.0 39.0 12 35.29738924697553 39.0 35.0 39.0 29.0 39.0 13 35.27264495943398 39.0 35.0 39.0 29.0 39.0 14 36.425822347511094 40.0 36.0 41.0 29.0 41.0 15 36.41125522780299 40.0 36.0 41.0 29.0 41.0 16 36.370818130650456 40.0 36.0 41.0 29.0 41.0 17 36.308042117275875 40.0 36.0 41.0 28.0 41.0 18 36.25712404732673 40.0 36.0 41.0 28.0 41.0 19 36.32748324745422 40.0 37.0 41.0 28.0 41.0 20 36.295448834528955 40.0 37.0 41.0 28.0 41.0 21 36.243542950799714 40.0 37.0 41.0 28.0 41.0 22 36.187082380088064 40.0 37.0 41.0 28.0 41.0 23 36.11362126563118 40.0 37.0 41.0 28.0 41.0 24 36.056216452381705 40.0 36.0 41.0 27.0 41.0 25 36.005096044745876 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3641641360990917 1101 2 0.36835110702098817 1101 3 0.31372586092706456 1101 4 0.45096282734570536 1101 5 0.3338695805304184 1101 6 0.3275180792227701 1101 7 0.446368577426 1101 8 0.3621667922061391 1101 9 0.4304669252158533 1101 10 0.5068086074515818 1101 11 0.5095100111965039 1101 12 0.4065422230769826 1101 13 0.5444719572160395 1101 14 0.6344104705064453 1101 15 0.7510492389685126 1101 16 0.6586821260500528 1101 17 0.6474430636826654 1101 18 0.5089654768649083 1101 19 0.6275997776742486 1101 20 0.6450704813594399 1101 21 0.6523407944194162 1101 22 0.7060099417222787 1101 23 0.6549834204764835 1101 24 0.7708719279078977 1101 25 0.6750927765848829 1102 1 0.47403626917617103 1102 2 0.595349005927261 1102 3 0.44928882710031104 1102 4 0.565756401297584 1102 5 0.6490948964779975 1102 6 0.6285115480091719 1102 7 0.6002060255199311 1102 8 0.7060881669172261 1102 9 0.688527739485437 1102 10 0.674494533036075 1102 11 0.6836783981725745 1102 12 0.7471182146384194 1102 13 0.735237623375447 1102 14 0.9491846702024702 1102 15 0.8031564900370256 1102 16 0.8689225599320807 1102 17 0.9725995851933078 1102 18 0.7900387599876808 1102 19 0.893375787850168 1102 20 0.8577618219218195 1102 21 0.8230246644966712 1102 22 0.7124977848515854 1102 23 0.7602894656558519 1102 24 0.8903214964349857 1102 25 0.8817651183467561 1103 1 0.34411301544599127 1103 2 0.31234092563759575 1103 3 0.3568095640815301 1103 4 0.445811629464707 1103 5 0.3722944646964379 1103 6 0.27995471824728213 1103 7 0.25088849936201996 1103 8 0.330944501288009 1103 9 0.37408600594304176 1103 10 0.45547343242526495 1103 11 0.36942750905050303 1103 12 0.28920737431258203 1103 13 0.27908570616975226 1103 14 0.5054953668114166 1103 15 0.5422098670132698 1103 16 0.5100896256107958 1103 17 0.547792207936638 1103 18 0.427361651239643 1103 19 0.5263603595771542 1103 20 0.5237239940341496 1103 21 0.442508649574215 1103 22 0.5278231596045728 1103 23 0.33633962650930727 1103 24 0.41285888183173114 1103 25 0.39510033278570234 1104 1 0.054980017466405684 1104 2 0.218768996561451 1104 3 0.18648001369068368 1104 4 0.3608069066336199 1104 5 0.2283181246925139 1104 6 0.25568320207973017 1104 7 0.2293314026885227 1104 8 0.2087224973544508 1104 9 0.24810585087701753 1104 10 0.23527022439274248 1104 11 0.2408921292921562 1104 12 0.34056274419803856 1104 13 0.319114233379274 1104 14 0.35989994862803343 1104 15 0.3980704341958159 1104 16 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-0.8559026208040734 2215 21 -0.8841157061184859 2215 22 -1.1088852002357328 2215 23 -0.8509670812995225 2215 24 -0.9579866196635152 2215 25 -0.9522169945098895 2216 1 -0.6866695370422882 2216 2 -0.7185659933438124 2216 3 -0.8026286010585579 2216 4 -0.5655331622751731 2216 5 -0.8355087865485942 2216 6 -1.0182953890288502 2216 7 -1.2296310540996842 2216 8 -1.1091437459660582 2216 9 -1.4300504075712155 2216 10 -1.2763919269650117 2216 11 -1.1838575155227105 2216 12 -1.1386380748847458 2216 13 -1.2847695074822525 2216 14 -1.397852290332466 2216 15 -1.383566926102823 2216 16 -1.381119219911895 2216 17 -1.5208672432268102 2216 18 -1.4899500362259204 2216 19 -1.7040995782042998 2216 20 -1.434436871055965 2216 21 -1.806441616987314 2216 22 -1.6975470593863662 2216 23 -1.6415801055908261 2216 24 -1.651073358809569 2216 25 -1.706986745583805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35655.0 3 0.0 4 616.0 5 4907.0 6 5469.0 7 4271.0 8 3397.0 9 3005.0 10 2844.0 11 2617.0 12 2381.0 13 2289.0 14 2382.0 15 2332.0 16 2318.0 17 2534.0 18 2827.0 19 3368.0 20 4320.0 21 5713.0 22 7362.0 23 8720.0 24 10346.0 25 11466.0 26 12950.0 27 14437.0 28 16523.0 29 20019.0 30 25308.0 31 32061.0 32 41371.0 33 53919.0 34 71373.0 35 95445.0 36 136024.0 37 218888.0 38 805960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.532618406087764 19.60506787503483 13.207198544791781 27.65511517408562 2 13.680923112370463 20.86851880481517 39.83867858023302 25.611879502581345 3 16.766298362657782 25.17511402183561 30.45446975742791 27.604117858078702 4 11.099074062468105 17.515159220309627 38.00265372156695 33.383112995655324 5 12.247648221202208 38.618394171746154 36.482976633939685 12.650980973111947 6 31.681383072689762 38.09791574969834 17.82652306568338 12.394178111928523 7 27.0914431734053 31.906359706732264 22.94748405894013 18.05471306092231 8 24.875490620382944 37.4545346008626 20.5831629531243 17.086811825630157 9 26.298752655464426 14.561346227196134 19.768772394988655 39.37112872235079 10 13.936250666281472 29.43492765362078 35.474467019589426 21.154354660508325 11 34.367742478439695 22.138667567536917 24.821402671693075 18.67218728233031 12 22.566978847654045 24.873210669343813 32.438968925348135 20.120841557654003 13 28.95577638283484 21.651266520514 25.92614256629842 23.466814530352742 14 20.664141888927688 21.91029578099493 27.77912074466021 29.646441585417172 15 23.141701439104416 30.82056586509508 23.22812768403329 22.80960501176722 16 22.201995085402622 28.032782286439733 27.668634137053644 22.096588491104004 17 21.616179344912524 28.649599957972004 27.80098073279151 21.933239964323963 18 21.742520582949734 27.686948111264353 29.28420889231192 21.286322413473997 19 23.26632712930572 27.19358822311102 27.428156691737044 22.111927955846216 20 23.437627766699276 27.5007953243879 27.718830595606946 21.34274631330588 21 22.854294931673238 26.99836755888875 27.086107538177334 23.06122997126068 22 22.988366478315548 27.99995463815874 26.93108641012954 22.080592473396177 23 22.183742356006412 27.66551167378465 27.39702805707515 22.75371791313379 24 22.897284675994094 27.386913228169462 27.76646052893101 21.949341566905435 25 22.71074492632866 27.267170979829462 27.473746787361215 22.54833730648067 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 107.0 1 107.0 2 175.5 3 244.0 4 244.0 5 244.0 6 647.5 7 1051.0 8 1051.0 9 1051.0 10 1573.5 11 2096.0 12 2096.0 13 2096.0 14 4193.5 15 6291.0 16 6291.0 17 6291.0 18 12360.5 19 18430.0 20 18430.0 21 18430.0 22 30062.5 23 41695.0 24 41695.0 25 41695.0 26 61279.5 27 80864.0 28 80864.0 29 80864.0 30 104773.5 31 128683.0 32 128683.0 33 128683.0 34 146186.0 35 163689.0 36 163689.0 37 163689.0 38 176784.0 39 189879.0 40 189879.0 41 189879.0 42 199426.0 43 208973.0 44 208973.0 45 208973.0 46 216511.0 47 224049.0 48 224049.0 49 224049.0 50 213967.5 51 203886.0 52 203886.0 53 203886.0 54 180412.5 55 156939.0 56 156939.0 57 156939.0 58 134310.5 59 111682.0 60 111682.0 61 111682.0 62 91055.0 63 70428.0 64 70428.0 65 70428.0 66 54256.0 67 38084.0 68 38084.0 69 38084.0 70 27744.5 71 17405.0 72 17405.0 73 17405.0 74 12160.0 75 6915.0 76 6915.0 77 6915.0 78 4794.0 79 2673.0 80 2673.0 81 2673.0 82 1812.0 83 951.0 84 951.0 85 951.0 86 629.0 87 307.0 88 307.0 89 307.0 90 195.5 91 84.0 92 84.0 93 84.0 94 46.5 95 9.0 96 9.0 97 9.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1837751437403345 2 0.007639889054486137 3 0.004774930659053836 4 0.00202934553009788 5 0.08147225437010606 6 0.3289927224088092 7 0.02966425671937195 8 0.014444165243637853 9 0.005849290057340949 10 0.004416810859624798 11 0.0 12 0.0 13 0.0 14 0.0013131059312398048 15 0.0 16 0.0 17 0.02047251520069332 18 0.029067390386990223 19 0.0 20 0.0 21 7.162395988580754E-4 22 0.0 23 0.004894303925530182 24 0.0 25 1.7905989971451884E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1675417.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.12765783933946 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56006065243346 57.19616026789628 2 12.468842310906169 17.488214144638132 3 3.240494754734601 6.81744922170607 4 1.1784175101137193 3.3055863976456505 5 0.5183659165698429 1.8175893816392767 6 0.2810247436002945 1.1824564238153734 7 0.16453801050165742 0.8077065711418111 8 0.10987932624888085 0.6164463835838934 9 0.08384449086661877 0.5291835990487078 >10 0.35429306035596714 4.364047439880311 >50 0.021277601548834405 1.0270823116264431 >100 0.01573097498848612 2.257986572096289 >500 0.00195539168489277 0.9604258572060783 >1k 0.001275255446669198 1.6296654280756686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 3412 0.2036507926086461 No Hit GTACATGGGAAGCAGTGGTATCAAC 3410 0.20353141934216973 No Hit GTCCTACAGTGGACATTTCTAAATT 2171 0.12957968076007348 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2161 0.12898281442769172 No Hit GTATCAACGCAGAGTACTTTTTTTT 2139 0.12766970849645193 No Hit CTTTAGGACGTGAAATATGGCGAGG 2104 0.1255806763331159 No Hit CTGTAGGACGTGGAATATGGCAAGA 2042 0.12188010507234914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.968663323817294E-5 7 0.0 0.0 0.0 0.0 5.968663323817294E-5 8 0.0 0.0 0.0 0.0 5.968663323817294E-5 9 0.0 0.0 0.0 0.0 5.968663323817294E-5 10 0.0 0.0 0.0 0.0 5.968663323817294E-5 11 0.0 0.0 0.0 0.0 5.968663323817294E-5 12 0.0 0.0 0.0 0.0 1.1937326647634588E-4 13 0.0 0.0 0.0 0.0 1.7905989971451884E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGT 140 0.0 14.9603815 6 GCGCCAC 105 9.094947E-11 14.461998 13 CGTGCGC 100 6.712071E-10 14.233055 10 GGCGTGC 145 0.0 13.089039 8 GCGTGCG 110 2.7957867E-9 12.93914 9 AGGCGTG 155 0.0 12.859884 7 TTAGTAC 60 4.0164398E-4 12.6947975 3 TAGGACC 460 0.0 12.418823 4 AAAGGCG 170 0.0 12.321421 5 GTGCGCC 125 1.4351826E-9 12.146628 11 GGCGAGG 665 0.0 11.84269 19 GTATTAA 195 0.0 11.233713 1 TGCGCCA 145 1.2369128E-9 11.127011 12 TCGAACC 60 0.0059282472 11.070154 9 CGTACTA 115 8.95574E-7 10.72604 19 GGACGTG 1285 0.0 10.594512 6 AGGACGT 1305 0.0 10.578998 5 AATGTCC 390 0.0 10.46284 8 TAGGACT 320 0.0 10.413701 4 GTCCTAC 1200 0.0 10.39729 1 >>END_MODULE