FastQCFastQC Report
Thu 26 May 2016
SRR1513149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89705
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC42054.687587091020568No Hit
CCCATGTACTCTGCGTTGATACCAC35593.9674488601527225No Hit
GAGTACATGGGAAGCAGTGGTATCA16701.861657655649072No Hit
TATCAACGCAGAGTACATGGGAAGC12331.3745053230031772No Hit
CATGTACTCTGCGTTGATACCACTG12241.3644724374338109No Hit
GTATCAACGCAGAGTACATGGGAAG11331.2630288166768855No Hit
GCGTTGATACCACTGCTTCCCATGT11061.2329301599687865No Hit
GGTATCAACGCAGAGTACATGGGAA9231.0289281533916728No Hit
ACGCAGAGTACATGGGAAGCAGTGG9021.0055180870631515No Hit
ACTCTGCGTTGATACCACTGCTTCC8120.9051892313694888No Hit
GTATCAACGCAGAGTACTTTTTTTT7840.8739758095981272No Hit
GGTATCAACGCAGAGTACTTTTTTT5180.5774483027701911No Hit
GTACTCTGCGTTGATACCACTGCTT5000.5573825316314587No Hit
GTGGTATCAACGCAGAGTACATGGG4880.5440053508723037No Hit
GGGAAGCAGTGGTATCAACGCAGAG4790.5339724653029374No Hit
TATCAACGCAGAGTACTTTTTTTTT4750.5295134050498858No Hit
CAGTGGTATCAACGCAGAGTACATG4220.4704308566969511No Hit
ATACCACTGCTTCCCATGTACTCTG4190.4670865615071624No Hit
GCTTCCCATGTACTCTGCGTTGATA4120.45928320606432194No Hit
GCAGAGTACATGGGAAGCAGTGGTA4030.44925032049495567No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3750.418036898723594No Hit
CTGCTTCCCATGTACTCTGCGTTGA3750.418036898723594No Hit
GCAGTGGTATCAACGCAGAGTACAT3570.39797112758486153No Hit
GATACCACTGCTTCCCATGTACTCT3510.391282537205284No Hit
CATGGGAAGCAGTGGTATCAACGCA3430.38236441669918064No Hit
TACCACTGCTTCCCATGTACTCTGC3350.3734462961930773No Hit
CCACTGCTTCCCATGTACTCTGCGT3250.3622986455604481No Hit
GTTGATACCACTGCTTCCCATGTAC3250.3622986455604481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3100.3455771696115044No Hit
AAGCAGTGGTATCAACGCAGAGTAC2820.3143637478401427No Hit
ACATGGGAAGCAGTGGTATCAACGC2740.30544562733403935No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2610.29095368151162143No Hit
CTTCCCATGTACTCTGCGTTGATAC2410.26865838024636307No Hit
ATGGGAAGCAGTGGTATCAACGCAG2200.2452483139178418No Hit
TGATACCACTGCTTCCCATGTACTC2200.2452483139178418No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2200.2452483139178418No Hit
GTACATGGGAAGCAGTTGTATCAAC2100.23410066328521262No Hit
CCCATGTACTCTGCGTTTATACCAC2060.22964160303216097No Hit
ATCAACGCAGAGTACATGGGAAGCA1970.21960871746279473No Hit
CCATGTACTCTGCGTTGATACCACT1930.21514965720974302No Hit
GTACATGGGAAGCAGTGTTATCAAC1900.2118053620199543No Hit
TTCCCATGTACTCTGCGTTGATACC1760.19619865113427343No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1470.16387046429964885No Hit
AAAAAGTACTCTGCGTTGATACCAC1420.15829663898333426No Hit
GTACATGGTAAGCAGTGGTATCAAC1350.15049328354049385No Hit
GGAAGCAGTGGTATCAACGCAGAGT1150.1281979822752355No Hit
GTACTGGTTCACTATCGGTCAGTCA1130.12596845214870966No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1120.12485368708544675No Hit
GAGTACATGTGAAGCAGTGGTATCA1060.11816509670586925No Hit
CATTTACTCTGCGTTGATACCACTG1050.11705033164260631No Hit
AAAGTACTCTGCGTTGATACCACTG980.1092469761997659No Hit
TGGGAAGCAGTGGTATCAACGCAGA930.1036731508834513No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA920.10255838582018839No Hit
ACCATGTACTCTGCGTTGATACCAC900.10032885569366257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA307.71524E-418.9743614
CAGTGTT307.71524E-418.9743613
GCAGTGT350.002168309916.26373712
TTATACC456.743587E-414.75783517
TTTATAC456.743587E-414.75783516
CGTTTAT400.005275021314.23076914
GCGTTTA400.005275021314.23076913
TATACCA500.001495572613.28205218
TGTTATC500.001495572613.28205216
GTTTATA500.001495572613.28205215
GTTATCA550.003060679212.07459217
GAATCAA700.001484447710.8424918
AATCAAC856.5457023E-410.04524919
GAAGCAG11450.08.9486069
AAGCAGT12100.08.93833410
AGCAGTG11700.08.83848311
GGAAGCA11550.08.7889888
AGTGGTA10850.08.74394414
CAGTGGT10850.08.74394413
GTGGTAT11150.08.59376815
Produced by FastQC (version 0.11.5)