FastQCFastQC Report
Thu 26 May 2016
SRR1513149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89705
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC47955.345298478345688No Hit
GTACATGGGAAGCAGTGGTATCAAC47135.2538877431581295No Hit
GAGTACATGGGAAGCAGTGGTATCA17331.9318878546346359No Hit
CATGTACTCTGCGTTGATACCACTG16051.7891979265369822No Hit
TATCAACGCAGAGTACATGGGAAGC14861.6565408840086953No Hit
GTATCAACGCAGAGTACTTTTTTTT14251.5885402151496573No Hit
ACGCAGAGTACATGGGAAGCAGTGG13071.4569979376846331No Hit
TATCAACGCAGAGTACTTTTTTTTT12801.4268992809765342No Hit
GCGTTGATACCACTGCTTCCCATGT12281.3689314976868625No Hit
GTATCAACGCAGAGTACATGGGAAG11591.2920127083217212No Hit
ACTCTGCGTTGATACCACTGCTTCC10431.1626999609832227No Hit
GGTATCAACGCAGAGTACATGGGAA9591.0690596956691378No Hit
GGTATCAACGCAGAGTACTTTTTTT9151.0200100328855692No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7030.7836798394738309No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6710.7480073574494176No Hit
GTACTCTGCGTTGATACCACTGCTT5570.6209241402374449No Hit
CTTATACACATCTCCGAGCCCACGA5510.6142355498578674No Hit
ATACCACTGCTTCCCATGTACTCTG5450.60754695947829No Hit
GCTTCCCATGTACTCTGCGTTGATA5250.5852516582130316No Hit
GTGGTATCAACGCAGAGTACATGGG5190.5785630678334541No Hit
GGGAAGCAGTGGTATCAACGCAGAG5010.5584972966947216No Hit
TACCACTGCTTCCCATGTACTCTGC4660.5194805194805194No Hit
CAGTGGTATCAACGCAGAGTACATG4600.512791929100942No Hit
GCAGTGGTATCAACGCAGAGTACAT4490.5005295134050499No Hit
CATGGGAAGCAGTGGTATCAACGCA4250.4737751518867399No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4200.46820132657042524No Hit
GCAGAGTACATGGGAAGCAGTGGTA4180.46597179644389947No Hit
GATACCACTGCTTCCCATGTACTCT4150.46262750125411073No Hit
ACATGGGAAGCAGTGGTATCAACGC4120.45928320606432194No Hit
CTGCTTCCCATGTACTCTGCGTTGA4120.45928320606432194No Hit
CCACTGCTTCCCATGTACTCTGCGT4020.44813555543169276No Hit
AAGCAGTGGTATCAACGCAGAGTAC3360.3745610612563402No Hit
CTTCCCATGTACTCTGCGTTGATAC3270.364528175686974No Hit
GTTGATACCACTGCTTCCCATGTAC3170.3533805250543448No Hit
CCATGTACTCTGCGTTGATACCACT3080.34334763948497854No Hit
ATACACATCTCCGAGCCCACGAGAC3030.3377738141686639No Hit
ATGGGAAGCAGTGGTATCAACGCAG2870.31993757315645727No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2490.27757650075246637No Hit
TCTCCGAGCCCACGAGACCGTACTA2460.2742322055626777No Hit
TTCCCATGTACTCTGCGTTGATACC2440.27200267543615186No Hit
ATCAACGCAGAGTACATGGGAAGCA2350.26196978986678554No Hit
TGATACCACTGCTTCCCATGTACTC2310.2575107296137339No Hit
ACACATCTCCGAGCCCACGAGACCG2140.23855972353826432No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1910.2129201270832172No Hit
GGAAGCAGTGGTATCAACGCAGAGT1880.20957583189342843No Hit
AAAAAGTACTCTGCGTTGATACCAC1750.19508388607101051No Hit
GTGGTATCAACGCAGAGTACTTTTT1640.18282147037511845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1630.18170670531185554No Hit
ATCAACGCAGAGTACTTTTTTTTTT1510.16832952455270053No Hit
AAAGTACTCTGCGTTGATACCACTG1450.16164093417312303No Hit
GTACATGGTAAGCAGTGGTATCAAC1370.15272281366701967No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1180.13154227746502425No Hit
CTGCGTTGATACCACTGCTTCCCAT1160.1293127473384984No Hit
ACCATGTACTCTGCGTTGATACCAC1150.1281979822752355No Hit
TGGGAAGCAGTGGTATCAACGCAGA1140.12708321721197258No Hit
GTACTGGTTCACTATCGGTCAGTCA1070.11927986176913216No Hit
TCCCATGTACTCTGCGTTGATACCA1030.11482080151608048No Hit
TCAACGCAGAGTACATGGGAAGCAG980.1092469761997659No Hit
CATCTCCGAGCCCACGAGACCGTAC960.10701744607324007No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT940.10478791594671422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA654.456524E-1019.0649852
CTTATAC701.1914381E-917.7131251
ATGGGTA350.00210026116.3414155
TATACAC807.066774E-915.4903023
CATGGGT853.683741E-612.3361664
ATACACA1104.5641536E-711.2656734
TACACAT1201.1607517E-711.1212425
ACATCTC1401.0339936E-69.4846718
CCATGTA11900.08.9717582
ACACATC1502.4656947E-68.8969936
CACATCT1502.6400976E-68.852367
CATCTCC1502.6400976E-68.852369
TGATACC12600.08.430819517
CCCATGT12000.08.3456071
TTGATAC12800.08.22498916
ATCTCCG1502.5397128E-58.22004810
CTGCGTT13050.08.21278111
TGCGTTG12950.08.20295912
GATACCA12900.08.16122918
GAAGCAG10150.08.1297189
Produced by FastQC (version 0.11.5)