##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89705 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.365263920628728 31.0 29.0 34.0 15.0 34.0 2 28.312424056630064 31.0 28.0 34.0 15.0 34.0 3 28.49663898333426 31.0 28.0 34.0 16.0 34.0 4 31.58693495345856 35.0 32.0 37.0 16.0 37.0 5 31.2322167103283 35.0 32.0 37.0 10.0 37.0 6 30.864923917284433 36.0 32.0 37.0 2.0 37.0 7 30.566256061535032 35.0 32.0 37.0 2.0 37.0 8 30.431469817735913 36.0 32.0 37.0 2.0 37.0 9 31.63014324731063 38.0 32.0 39.0 2.0 39.0 10 31.54780669973803 38.0 32.0 39.0 2.0 39.0 11 31.53513182096873 38.0 32.0 39.0 2.0 39.0 12 31.44370993813054 38.0 32.0 39.0 2.0 39.0 13 31.31745164706538 38.0 32.0 39.0 2.0 39.0 14 32.2174906638426 39.0 32.0 41.0 2.0 41.0 15 32.13908923694331 39.0 32.0 41.0 2.0 41.0 16 31.945108968284934 38.0 31.0 41.0 2.0 41.0 17 32.07775486316259 39.0 32.0 41.0 2.0 41.0 18 32.053319212975865 39.0 31.0 41.0 2.0 41.0 19 32.01584081154897 39.0 31.0 41.0 2.0 41.0 20 32.15238838414804 39.0 32.0 41.0 2.0 41.0 21 32.07903684298534 39.0 32.0 41.0 2.0 41.0 22 32.10643776824034 39.0 32.0 41.0 2.0 41.0 23 32.02141463686528 39.0 32.0 41.0 2.0 41.0 24 31.92571205618416 39.0 32.0 41.0 2.0 41.0 25 31.887419876261077 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03858667152664452 1101 2 0.28548600212459974 1101 3 0.13883299243810399 1101 4 0.3129897476991417 1101 5 0.9525649257774731 1101 6 -0.19999336747350327 1101 7 -0.1440779377960233 1101 8 0.10013752291892786 1101 9 0.17064105949209463 1101 10 0.1094344957774318 1101 11 0.5562797102257804 1101 12 0.7879106236817996 1101 13 0.6041472911012029 1101 14 0.8296262343819691 1101 15 0.5361029171181926 1101 16 0.4944782533729466 1101 17 0.3775717853237879 1101 18 0.46087118158779816 1101 19 0.4058055435151573 1101 20 0.6999152891752587 1101 21 0.9393367286616368 1101 22 0.01353671449930971 1101 23 0.048028925917307674 1101 24 0.2778026930174988 1101 25 0.7304930201351496 1102 1 1.4808192296661815 1102 2 1.4409588703416532 1102 3 1.3170655505776416 1102 4 1.8466331585518532 1102 5 1.609557173839491 1102 6 1.6124097333016927 1102 7 1.7222786513512673 1102 8 1.8905251198181539 1102 9 1.7192922222827924 1102 10 1.6247833329867376 1102 11 1.802915369140507 1102 12 1.7180191508135856 1102 13 2.040798453891899 1102 14 2.759424683994368 1102 15 2.537591289211221 1102 16 2.141051896783793 1102 17 2.160951630285034 1102 18 2.1642510265490387 1102 19 2.08940244273996 1102 20 1.6714501728961864 1102 21 2.5493212247856647 1102 22 2.271459195119462 1102 23 2.5484940421963813 1102 24 2.720035251157036 1102 25 2.5161674387397994 1103 1 1.824780219913741 1103 2 1.8559376150278233 1103 3 1.9513491214703649 1103 4 2.2729897476991425 1103 5 2.6889520225516677 1103 6 2.5806517938168163 1103 7 2.578180126720106 1103 8 2.541750426144734 1103 9 3.243673317556617 1103 10 3.269757076422593 1103 11 2.5828603553870693 1103 12 2.9935880430366417 1103 13 2.6296956781979794 1103 14 3.1543359118013186 1103 15 2.77765130421497 1103 16 2.8787363178890715 1103 17 3.0286685595173424 1103 18 3.1368066654587636 1103 19 2.7098700596441887 1103 20 3.0561088375623555 1103 21 3.2968205996293776 1103 22 3.1379883274025318 1103 23 3.07512570011086 1103 24 2.918834951082019 1103 25 2.665267213683535 1104 1 1.1307393432823716 1104 2 0.6105547044146746 1104 3 1.1349245954915403 1104 4 0.9218447095312001 1104 5 1.4039237044034323 1104 6 0.9313043424501615 1104 7 0.6928686270894744 1104 8 1.0729619503998435 1104 9 1.1451754106371332 1104 10 1.0385948011209436 1104 11 0.9778369621341767 1104 12 0.6350556618497443 1104 13 0.5735518712538727 1104 14 0.39768730308425404 1104 15 0.8818128407823167 1104 16 0.8617454289454614 1104 17 0.9087473578428771 1104 18 0.8128101128855079 1104 19 0.6483704290113366 1104 20 0.9212282662744968 1104 21 0.9014130645394971 1104 22 1.024284806102358 1104 23 1.0468075518715096 1104 24 1.0111767388190245 1104 25 1.238340348379424 1105 1 0.292774171526645 1105 2 -0.13020149787540092 1105 3 0.4024579924381051 1105 4 -0.08638525230085747 1105 5 -0.4063100742225245 1105 6 0.648444132526496 1105 7 0.04592206220397799 1105 8 -0.0817374770810737 1105 9 0.44276605949209724 1105 10 0.3388094957774328 1105 11 0.150904710225781 1105 12 0.35422312368180187 1105 13 0.21739729110120365 1105 14 0.7531262343819662 1105 15 -0.0955845828818056 1105 16 -0.30195924662705664 1105 17 0.15719678532379078 1105 18 0.24643368158779566 1105 19 0.21743054351515667 1105 20 -0.09308471082474057 1105 21 0.2050867286616338 1105 22 -0.07471328550069245 1105 23 0.32084142591730824 1105 24 0.24292769301749928 1105 25 0.4903680201351506 1106 1 1.9739443357602227 1106 2 1.965544396285182 1106 3 1.9196797077665728 1106 4 1.8934277039035194 1106 5 2.377791203149741 1106 6 2.3576708661031383 1106 7 1.9017614782623724 1106 8 1.953276209050312 1106 9 2.3336191616818773 1106 10 2.2440330359234153 1106 11 2.80042569562724 1106 12 2.2985091638277844 1106 13 1.8668042254077726 1106 14 1.908691927812626 1106 15 2.41621970543936 1106 16 2.0727556256357147 1106 17 2.480987843717955 1106 18 3.0244332253834187 1106 19 2.5631194121282945 1106 20 2.7289955811460658 1106 21 3.217176144720028 1106 22 2.968398028367922 1106 23 2.9654011886910325 1106 24 3.0890727660101973 1106 25 2.9206828011570494 1107 1 1.6934438143837873 1107 2 1.459260438214823 1107 3 1.6199156992050234 1107 4 1.919606289052524 1107 5 1.760865677657172 1107 6 1.9323374595941623 1107 7 1.5665987539332988 1107 8 2.392769101866296 1107 9 1.7537388038530004 1107 10 2.1467277288601423 1107 11 2.0497533944363084 1107 12 2.524512127441202 1107 13 2.1955457873418034 1107 14 2.0092352569383607 1107 15 1.6893660750129342 1107 16 2.0494857721699375 1107 17 1.754353740211009 1107 18 2.3892320838434316 1107 19 2.431128851785836 1107 20 2.283794988423381 1107 21 1.8293818414435918 1107 22 1.9198825791609622 1107 23 1.1466755424586594 1107 24 1.9176222418896813 1107 25 2.247064448706581 1108 1 0.5148723858123603 1108 2 0.6494860021246005 1108 3 0.3225472781523919 1108 4 0.03727546198485854 1108 5 0.847707782920331 1108 6 0.33572091824078143 1108 7 0.031636347918261976 1108 8 0.09728038006178252 1108 9 0.3003553452063805 1108 10 -0.1419940756511373 1108 11 0.06742256736863794 1108 12 0.9267677665389478 1108 13 0.4575758625297759 1108 14 0.7870548058105342 1108 15 1.1483886314039111 1108 16 1.3679068248015156 1108 17 1.3415717853237936 1108 18 1.2020140387306526 1108 19 0.6946626863722969 1108 20 0.35334386060382883 1108 21 0.3613367286616338 1108 22 0.5172510002135908 1108 23 0.945171783060168 1108 24 0.9326598358746452 1108 25 0.88992159156372 1109 1 0.12305043964258644 1109 2 0.46977585719706383 1109 3 0.6114706735975268 1109 4 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-0.6137060789123261 2216 25 -1.3160099038414579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7127.0 3 0.0 4 105.0 5 896.0 6 1203.0 7 807.0 8 585.0 9 433.0 10 380.0 11 346.0 12 304.0 13 220.0 14 251.0 15 218.0 16 226.0 17 172.0 18 227.0 19 329.0 20 445.0 21 538.0 22 592.0 23 705.0 24 824.0 25 802.0 26 877.0 27 853.0 28 949.0 29 1056.0 30 1388.0 31 1652.0 32 2114.0 33 2662.0 34 3478.0 35 4782.0 36 6882.0 37 11862.0 38 33415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40305307708629 18.27044411439547 12.54508704731488 25.781415761203363 2 11.28800294324225 22.781140952317248 35.25758944000357 30.673266664436937 3 19.24324685893934 30.205464943867828 27.84200492759117 22.70928326960167 4 11.755459683622622 20.147598185122014 27.968964248687335 40.127977882568025 5 10.321551300932743 40.80309724639621 34.787120096398446 14.088231356272592 6 32.63142591950617 30.845531910134977 19.274683247039352 17.248358923319504 7 23.040467455423354 32.88245592515361 22.35913333407674 21.717943285346298 8 30.996499208419735 30.87274511115571 21.098401230851564 17.032354449572992 9 28.114827201783722 13.337792642140467 18.648829431438127 39.89855072463768 10 16.851352255245146 30.997079217854672 30.69162337517558 21.4599451517246 11 28.158965498021292 25.53369377403712 18.327852405105624 27.97948832283596 12 24.83027701911822 30.637088233654758 27.39312189955967 17.139512847667355 13 32.86996265537038 18.77375843041079 22.781338832840976 25.57494008137785 14 22.095382488684756 26.55236226617021 25.891284475262538 25.4609707698825 15 35.35588874644668 21.480408004013153 22.35103951842149 20.812663731118665 16 18.525165821303162 25.437823978596512 33.52098545231593 22.516024747784407 17 24.434434546042436 29.180835999955402 27.507275139649234 18.87745431435293 18 26.71922566544376 20.704305451788084 31.349175373841675 21.227293508926483 19 21.9029039629898 29.526782230644894 32.668190178919794 15.902123627445516 20 23.379967671813166 23.42678780447021 30.51892313694889 22.674321386767737 21 19.999331126049295 26.4205210528076 33.189525433931976 20.39062238721113 22 21.256340226297308 26.625048770971517 24.087843486985115 28.030767515746057 23 16.917691390093534 26.50419737120815 30.44292578512581 26.1351854535725 24 21.50047377515189 33.376065994091746 26.436653475280085 18.686806755476283 25 20.87954963491444 22.149267041970905 25.30628170113149 31.664901621983166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 372.0 1 372.0 2 348.0 3 324.0 4 324.0 5 324.0 6 585.0 7 846.0 8 846.0 9 846.0 10 716.5 11 587.0 12 587.0 13 587.0 14 427.5 15 268.0 16 268.0 17 268.0 18 540.0 19 812.0 20 812.0 21 812.0 22 1278.0 23 1744.0 24 1744.0 25 1744.0 26 2523.5 27 3303.0 28 3303.0 29 3303.0 30 3673.0 31 4043.0 32 4043.0 33 4043.0 34 4137.0 35 4231.0 36 4231.0 37 4231.0 38 4292.0 39 4353.0 40 4353.0 41 4353.0 42 5183.0 43 6013.0 44 6013.0 45 6013.0 46 15336.5 47 24660.0 48 24660.0 49 24660.0 50 21650.5 51 18641.0 52 18641.0 53 18641.0 54 13466.5 55 8292.0 56 8292.0 57 8292.0 58 6276.0 59 4260.0 60 4260.0 61 4260.0 62 3874.0 63 3488.0 64 3488.0 65 3488.0 66 2612.0 67 1736.0 68 1736.0 69 1736.0 70 1369.5 71 1003.0 72 1003.0 73 1003.0 74 735.0 75 467.0 76 467.0 77 467.0 78 319.5 79 172.0 80 172.0 81 172.0 82 113.0 83 54.0 84 54.0 85 54.0 86 42.0 87 30.0 88 30.0 89 30.0 90 18.0 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17390334986901512 2 0.00891812050610334 3 0.006688590379577504 4 0.002229530126525835 5 0.08583690987124463 6 0.3143637478401427 7 0.031213421771361688 8 0.01226241569589209 9 0.005573825316314587 10 0.003344295189788752 11 0.0 12 0.0 13 0.0 14 0.003344295189788752 15 0.0 16 0.0 17 0.01783624101220668 18 0.031213421771361688 19 0.0 20 0.0 21 0.002229530126525835 22 0.0 23 0.006688590379577504 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 89705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.04124630734073 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07528049221861 33.66479014547684 2 9.170633926334252 7.344072236776099 3 2.984492886773017 3.585084443453542 4 1.3196358473231438 2.113594559946491 5 0.6208413374537153 1.2429630455381528 6 0.3563573596146886 0.8561395685859206 7 0.1920988891672931 0.538431525555989 8 0.1726105960633648 0.552923471378407 9 0.12249784236754921 0.4414469650521152 >10 0.7572593891812133 5.61730115378184 >50 0.06960104679974387 1.9240844991917954 >100 0.10022550739163118 10.660498299983278 >500 0.027840418719897547 7.185775597792765 >1k 0.030624460591887302 24.272894487486763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 4795 5.345298478345688 No Hit GTACATGGGAAGCAGTGGTATCAAC 4713 5.2538877431581295 No Hit GAGTACATGGGAAGCAGTGGTATCA 1733 1.9318878546346359 No Hit CATGTACTCTGCGTTGATACCACTG 1605 1.7891979265369822 No Hit TATCAACGCAGAGTACATGGGAAGC 1486 1.6565408840086953 No Hit GTATCAACGCAGAGTACTTTTTTTT 1425 1.5885402151496573 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1307 1.4569979376846331 No Hit TATCAACGCAGAGTACTTTTTTTTT 1280 1.4268992809765342 No Hit GCGTTGATACCACTGCTTCCCATGT 1228 1.3689314976868625 No Hit GTATCAACGCAGAGTACATGGGAAG 1159 1.2920127083217212 No Hit ACTCTGCGTTGATACCACTGCTTCC 1043 1.1626999609832227 No Hit GGTATCAACGCAGAGTACATGGGAA 959 1.0690596956691378 No Hit GGTATCAACGCAGAGTACTTTTTTT 915 1.0200100328855692 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 703 0.7836798394738309 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 671 0.7480073574494176 No Hit GTACTCTGCGTTGATACCACTGCTT 557 0.6209241402374449 No Hit CTTATACACATCTCCGAGCCCACGA 551 0.6142355498578674 No Hit ATACCACTGCTTCCCATGTACTCTG 545 0.60754695947829 No Hit GCTTCCCATGTACTCTGCGTTGATA 525 0.5852516582130316 No Hit GTGGTATCAACGCAGAGTACATGGG 519 0.5785630678334541 No Hit GGGAAGCAGTGGTATCAACGCAGAG 501 0.5584972966947216 No Hit TACCACTGCTTCCCATGTACTCTGC 466 0.5194805194805194 No Hit CAGTGGTATCAACGCAGAGTACATG 460 0.512791929100942 No Hit GCAGTGGTATCAACGCAGAGTACAT 449 0.5005295134050499 No Hit CATGGGAAGCAGTGGTATCAACGCA 425 0.4737751518867399 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 420 0.46820132657042524 No Hit GCAGAGTACATGGGAAGCAGTGGTA 418 0.46597179644389947 No Hit GATACCACTGCTTCCCATGTACTCT 415 0.46262750125411073 No Hit ACATGGGAAGCAGTGGTATCAACGC 412 0.45928320606432194 No Hit CTGCTTCCCATGTACTCTGCGTTGA 412 0.45928320606432194 No Hit CCACTGCTTCCCATGTACTCTGCGT 402 0.44813555543169276 No Hit AAGCAGTGGTATCAACGCAGAGTAC 336 0.3745610612563402 No Hit CTTCCCATGTACTCTGCGTTGATAC 327 0.364528175686974 No Hit GTTGATACCACTGCTTCCCATGTAC 317 0.3533805250543448 No Hit CCATGTACTCTGCGTTGATACCACT 308 0.34334763948497854 No Hit ATACACATCTCCGAGCCCACGAGAC 303 0.3377738141686639 No Hit ATGGGAAGCAGTGGTATCAACGCAG 287 0.31993757315645727 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 249 0.27757650075246637 No Hit TCTCCGAGCCCACGAGACCGTACTA 246 0.2742322055626777 No Hit TTCCCATGTACTCTGCGTTGATACC 244 0.27200267543615186 No Hit ATCAACGCAGAGTACATGGGAAGCA 235 0.26196978986678554 No Hit TGATACCACTGCTTCCCATGTACTC 231 0.2575107296137339 No Hit ACACATCTCCGAGCCCACGAGACCG 214 0.23855972353826432 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 191 0.2129201270832172 No Hit GGAAGCAGTGGTATCAACGCAGAGT 188 0.20957583189342843 No Hit AAAAAGTACTCTGCGTTGATACCAC 175 0.19508388607101051 No Hit GTGGTATCAACGCAGAGTACTTTTT 164 0.18282147037511845 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 163 0.18170670531185554 No Hit ATCAACGCAGAGTACTTTTTTTTTT 151 0.16832952455270053 No Hit AAAGTACTCTGCGTTGATACCACTG 145 0.16164093417312303 No Hit GTACATGGTAAGCAGTGGTATCAAC 137 0.15272281366701967 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 118 0.13154227746502425 No Hit CTGCGTTGATACCACTGCTTCCCAT 116 0.1293127473384984 No Hit ACCATGTACTCTGCGTTGATACCAC 115 0.1281979822752355 No Hit TGGGAAGCAGTGGTATCAACGCAGA 114 0.12708321721197258 No Hit GTACTGGTTCACTATCGGTCAGTCA 107 0.11927986176913216 No Hit TCCCATGTACTCTGCGTTGATACCA 103 0.11482080151608048 No Hit TCAACGCAGAGTACATGGGAAGCAG 98 0.1092469761997659 No Hit CATCTCCGAGCCCACGAGACCGTAC 96 0.10701744607324007 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 94 0.10478791594671422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACA 65 4.456524E-10 19.064985 2 CTTATAC 70 1.1914381E-9 17.713125 1 ATGGGTA 35 0.002100261 16.341415 5 TATACAC 80 7.066774E-9 15.490302 3 CATGGGT 85 3.683741E-6 12.336166 4 ATACACA 110 4.5641536E-7 11.265673 4 TACACAT 120 1.1607517E-7 11.121242 5 ACATCTC 140 1.0339936E-6 9.484671 8 CCATGTA 1190 0.0 8.971758 2 ACACATC 150 2.4656947E-6 8.896993 6 CACATCT 150 2.6400976E-6 8.85236 7 CATCTCC 150 2.6400976E-6 8.85236 9 TGATACC 1260 0.0 8.4308195 17 CCCATGT 1200 0.0 8.345607 1 TTGATAC 1280 0.0 8.224989 16 ATCTCCG 150 2.5397128E-5 8.220048 10 CTGCGTT 1305 0.0 8.212781 11 TGCGTTG 1295 0.0 8.202959 12 GATACCA 1290 0.0 8.161229 18 GAAGCAG 1015 0.0 8.129718 9 >>END_MODULE