FastQCFastQC Report
Thu 26 May 2016
SRR1513148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329861
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27780.11923458094710371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT701.0578028E-412.25524
GTCCTAC5300.010.4545011
CGCTATT750.002675780610.12078511
GTATTAC2002.1827873E-1110.0309141
GTATTGG3150.010.0081681
TAGAACT4100.09.996794
GTTACAC2201.8189894E-129.9653023
TAGACTG2400.09.9082545
CGCAGAT1554.1496605E-89.7934668
TAGGACG3700.09.789444
GTAGGAC4500.09.7438513
GCTACAC3050.09.6882913
TAAGGCT3050.09.6880834
TAGAACA5050.09.6261974
GTGTTAC2201.4551915E-119.5532511
GTTAGAC1304.062211E-69.5320293
TTAGGAC3500.09.5320283
CCTATAC2201.6370905E-119.5320283
AAGACCG800.00447037639.5119245
TCGAACC800.00456408749.4880319
Produced by FastQC (version 0.11.5)