##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513148_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2329861
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46948938155538	31.0	30.0	34.0	25.0	34.0
2	29.328133738450493	31.0	30.0	34.0	22.0	34.0
3	29.50117324595759	31.0	30.0	34.0	23.0	34.0
4	32.5713134817914	37.0	33.0	37.0	22.0	37.0
5	32.72226926842417	37.0	33.0	37.0	25.0	37.0
6	33.06930155919173	37.0	35.0	37.0	27.0	37.0
7	33.048082696778906	37.0	35.0	37.0	26.0	37.0
8	32.91611430896521	37.0	35.0	37.0	26.0	37.0
9	34.15665913116705	39.0	35.0	39.0	25.0	39.0
10	33.721816880921224	38.0	34.0	39.0	19.0	39.0
11	34.1971928797469	39.0	35.0	39.0	26.0	39.0
12	34.18826015800943	39.0	35.0	39.0	25.0	39.0
13	34.231577763652	39.0	35.0	39.0	26.0	39.0
14	34.4473738991296	39.0	34.0	41.0	17.0	41.0
15	34.881504089728956	40.0	34.0	41.0	22.0	41.0
16	34.72223579003211	39.0	34.0	41.0	18.0	41.0
17	34.31324958870937	39.0	33.0	41.0	17.0	41.0
18	34.03121001639153	39.0	33.0	41.0	17.0	41.0
19	34.68361417269099	39.0	34.0	41.0	19.0	41.0
20	34.89829736623773	40.0	34.0	41.0	23.0	41.0
21	34.94649809580915	40.0	35.0	41.0	23.0	41.0
22	34.625901287673386	40.0	34.0	41.0	18.0	41.0
23	34.15505946492087	39.0	34.0	41.0	10.0	41.0
24	34.40313477928512	39.0	34.0	41.0	17.0	41.0
25	34.040917891668215	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9185131742927695
1101	2	1.1489416460572954
1101	3	1.0451570934338044
1101	4	1.3529151077846322
1101	5	1.2772084432522774
1101	6	1.0421490974279948
1101	7	1.025540505134991
1101	8	1.2127991466875443
1101	9	1.3849015783333058
1101	10	1.6683787630930524
1101	11	1.3285651594124488
1101	12	1.2144629018742208
1101	13	1.1155886421373395
1101	14	2.038087565306178
1101	15	1.7650084663887142
1101	16	1.8221324220487034
1101	17	2.1892542650888416
1101	18	2.1042047760509917
1101	19	1.7389761534268828
1101	20	1.415945441653001
1101	21	1.3834899227038662
1101	22	1.697362205805831
1101	23	2.0878050436495172
1101	24	1.6626763293531042
1101	25	1.9378964241624885
1102	1	1.0018452145043
1102	2	1.1553122770216113
1102	3	1.0584567323466665
1102	4	1.3578026888078014
1102	5	1.3753089130287535
1102	6	1.1516374548442911
1102	7	1.0463937088575364
1102	8	1.1245741572499028
1102	9	1.3962866141153185
1102	10	1.6080831076384072
1102	11	1.0804001831285888
1102	12	1.0933912206797132
1102	13	1.1086332964431023
1102	14	2.0962217641727676
1102	15	1.718024612181722
1102	16	1.8110973259960375
1102	17	2.193958740064758
1102	18	2.3970615516415705
1102	19	1.7784071977277236
1102	20	1.565585608493393
1102	21	1.6320249694781097
1102	22	1.728822307518179
1102	23	2.0146796347958613
1102	24	1.7589256078959892
1102	25	2.0876954562587215
1103	1	0.9906778102542013
1103	2	1.2706550934258551
1103	3	1.0886634853175572
1103	4	1.5992109837047792
1103	5	1.4140015631903964
1103	6	1.1409355643575907
1103	7	1.1735967814782597
1103	8	1.2398305210980851
1103	9	1.5925259525570397
1103	10	1.8796813247617408
1103	11	1.3905041880986602
1103	12	1.3822981754799741
1103	13	1.3705071198816938
1103	14	2.2122461025787743
1103	15	1.9316121789741203
1103	16	1.8899930419640043
1103	17	2.347334851950947
1103	18	2.521628601263778
1103	19	1.8004466061360844
1103	20	1.5632857077783626
1103	21	1.596603066636824
1103	22	1.829474757487553
1103	23	2.267091624164415
1103	24	1.9572569668324675
1103	25	2.14293609444443
1104	1	0.7925774989274359
1104	2	1.0510637740349367
1104	3	1.0022259786395722
1104	4	1.3583487953475952
1104	5	1.1988857801387454
1104	6	0.9629489847678059
1104	7	0.9388138252524172
1104	8	1.0124460003267899
1104	9	1.3323109356285983
1104	10	1.4844805039096158
1104	11	1.0277147916419338
1104	12	1.024706312878088
1104	13	0.9642686763687038
1104	14	1.998419262900235
1104	15	1.5348057863761468
1104	16	1.615342479137091
1104	17	2.076156434880751
1104	18	2.2346490255066485
1104	19	1.4956258127381332
1104	20	1.2765176254190962
1104	21	1.276100930904228
1104	22	1.4501099228121035
1104	23	1.714372266034232
1104	24	1.5971850117705344
1104	25	1.8016545080726658
1105	1	0.7749188109343663
1105	2	1.0755137389821883
1105	3	0.9604356909790361
1105	4	1.3190711506969919
1105	5	1.201918474054544
1105	6	0.7916021856416435
1105	7	0.9005350822008822
1105	8	0.8705532820979798
1105	9	1.1876350046137105
1105	10	1.4838719458274952
1105	11	1.0190645382931933
1105	12	0.9703000478402117
1105	13	0.9081333051718872
1105	14	1.6662549245341225
1105	15	1.3153732093842692
1105	16	1.5547856574485124
1105	17	1.8625742066657693
1105	18	1.5137307167346634
1105	19	1.4017188030837673
1105	20	1.1300134146108007
1105	21	1.1386104472576974
1105	22	1.3153207400663618
1105	23	1.6558200056814556
1105	24	1.4732848172501605
1105	25	1.68412617885528
1106	1	0.524183398887768
1106	2	0.7597514295580368
1106	3	0.7185049645388091
1106	4	1.099493445353005
1106	5	0.9827366222174305
1106	6	0.5686109271386073
1106	7	0.5544941599200897
1106	8	0.5606807004602246
1106	9	0.7765750634370292
1106	10	0.7692221101702117
1106	11	0.6766094506631717
1106	12	0.6863746023944799
1106	13	0.5852070441425141
1106	14	1.614297945867527
1106	15	1.108843797123022
1106	16	1.1731988749681648
1106	17	1.4328011029178214
1106	18	1.539755592638528
1106	19	1.0340512878724724
1106	20	0.8408654201466419
1106	21	0.7380000257147117
1106	22	0.9188538371256314
1106	23	1.3581351135945496
1106	24	1.006485743856075
1106	25	1.3378115999259563
1107	1	0.5039057261730058
1107	2	0.7633310345289495
1107	3	0.7700896879442176
1107	4	1.0178828509162798
1107	5	0.8697900700321455
1107	6	0.6097087512148107
1107	7	0.6407393883899317
1107	8	0.6321374551480972
1107	9	0.8898925371643998
1107	10	1.0408812928152926
1107	11	0.7424205312569185
1107	12	0.6398999869938535
1107	13	0.5373857368915154
1107	14	1.391561209435089
1107	15	0.8683443020146342
1107	16	1.0765156063811432
1107	17	1.3913812165115615
1107	18	1.7142259473901902
1107	19	1.1053393133201084
1107	20	0.9248356317376647
1107	21	0.7008838346594857
1107	22	1.14967433804253
1107	23	1.3141051869344267
1107	24	1.1165778026238087
1107	25	1.4302357905774343
1108	1	0.47915228505164364
1108	2	0.6562564848880257
1108	3	0.5619196085387763
1108	4	0.9414555162183262
1108	5	0.8410847193244493
1108	6	0.48626483576600066
1108	7	0.3870724514890753
1108	8	0.47169659463793323
1108	9	0.7593625827309367
1108	10	1.0869305982211586
1108	11	0.6486143255800485
1108	12	0.4804055621566121
1108	13	0.44592875205862725
1108	14	1.5609487377642637
1108	15	1.0551093644381027
1108	16	1.1086330012082328
1108	17	1.3289224387591148
1108	18	1.5942460572805288
1108	19	1.1002392557632916
1108	20	0.8203426063040666
1108	21	0.7650092356953806
1108	22	1.1256615310024003
1108	23	1.4429723714577705
1108	24	1.0483920470665566
1108	25	1.3512435247695365
1109	1	0.3154933914085447
1109	2	0.5504320386205954
1109	3	0.4506776075259822
1109	4	0.6788801963137843
1109	5	0.6046147532590211
1109	6	0.4358462967955532
1109	7	0.2937624193314434
1109	8	0.4420508560186036
1109	9	0.534100852635909
1109	10	0.9743479633422965
1109	11	0.5136741623839143
1109	12	0.46125406464323504
1109	13	0.42293336112034297
1109	14	1.326228008100287
1109	15	0.7544515190653556
1109	16	0.9218766052364344
1109	17	1.2870941284271353
1109	18	1.5142291072606398
1109	19	0.8843784449690304
1109	20	0.7244304211579831
1109	21	0.5217221373949954
1109	22	0.8072076559967911
1109	23	1.1414068256774854
1109	24	0.8484794500214932
1109	25	1.1566526500273255
1110	1	0.3116287191472722
1110	2	0.554472589296843
1110	3	0.4248453490051851
1110	4	0.8397907998280019
1110	5	0.6065005607084473
1110	6	0.2841804769508869
1110	7	0.2625537716968722
1110	8	0.27780088905396383
1110	9	0.4599876427710825
1110	10	0.8484422825625515
1110	11	0.4131159112667575
1110	12	0.20636948368984065
1110	13	0.2462053324289002
1110	14	1.0192978402061925
1110	15	0.6751089507193768
1110	16	0.8308688312243717
1110	17	1.1140790884962257
1110	18	0.6882440485507502
1110	19	0.4974412791612792
1110	20	0.45342690917312467
1110	21	0.4184759243643654
1110	22	0.7259913776584952
1110	23	-0.6056953680567716
1110	24	0.09478832974113516
1110	25	0.5480109711236167
1111	1	0.11415427517231791
1111	2	0.5658828938729457
1111	3	0.41698206063796306
1111	4	0.9292354609995073
1111	5	0.6295183264215893
1111	6	0.2929131761998889
1111	7	0.3570229971043304
1111	8	0.3925321930411556
1111	9	0.6318333855557938
1111	10	0.8684120048405788
1111	11	0.4724819590750471
1111	12	0.3693080444199168
1111	13	0.2869852075040171
1111	14	1.1304810768832567
1111	15	0.70891506938991
1111	16	0.8311377629841772
1111	17	1.2207020473702386
1111	18	1.5226993573778103
1111	19	0.85609293850478
1111	20	0.45934581040594935
1111	21	0.27285125339658833
1111	22	0.657487656309641
1111	23	1.0345502172429448
1111	24	0.7179535199108713
1111	25	0.9953796292667576
1112	1	0.08427373766968671
1112	2	0.239348810836173
1112	3	0.11287487336143087
1112	4	0.5852501149796581
1112	5	0.32160791587476467
1112	6	0.0906974037767796
1112	7	-0.07423511973227903
1112	8	0.1341861492381895
1112	9	0.25563436377097304
1112	10	0.49958045170763654
1112	11	0.0716857726126392
1112	12	0.0649980234665577
1112	13	-0.007761143142737126
1112	14	0.568441594180392
1112	15	0.2802087926830481
1112	16	0.26850674939910135
1112	17	0.8149839302015707
1112	18	0.8281788492842281
1112	19	0.40418810180682385
1112	20	0.1683516991208549
1112	21	0.15813716513213194
1112	22	0.20444148531137074
1112	23	0.5673416126872155
1112	24	0.30287184795015776
1112	25	0.6476252206646649
1113	1	0.28677092312086216
1113	2	0.2979291009002658
1113	3	0.2474250238386162
1113	4	0.5087461157449198
1113	5	0.5771863884393511
1113	6	0.28675894857776996
1113	7	0.16960230604089332
1113	8	0.19821253609332956
1113	9	0.5161439994616615
1113	10	0.734322653751768
1113	11	0.2760384999656438
1113	12	0.1818765600483374
1113	13	0.045727057827086526
1113	14	0.7145168989978714
1113	15	0.514559386129747
1113	16	0.6496614134560232
1113	17	0.8345987233230332
1113	18	0.782872633730257
1113	19	0.5613584684707931
1113	20	0.4132366521381243
1113	21	0.31030359510688754
1113	22	0.4972154899176644
1113	23	0.5562918779538464
1113	24	0.5357866903722126
1113	25	0.9714581578763131
1114	1	-1.0708357387852416
1114	2	-0.7485201666218622
1114	3	-0.7681081233998945
1114	4	-0.4296634948264213
1114	5	-3.4252474865885993
1114	6	-1.2985557175695597
1114	7	-1.0260515143126838
1114	8	-0.8342584857692117
1114	9	-0.6115132975471198
1114	10	-0.39057943007745877
1114	11	-0.7197841761933077
1114	12	-0.9223761394085273
1114	13	-0.8087864628994055
1114	14	-6.321312990138665
1114	15	-2.699555833974266
1114	16	-1.5307979089568633
1114	17	-0.5952451878419325
1114	18	-6.7977919700248215
1114	19	-2.758210988896902
1114	20	-1.6902067512332195
1114	21	-1.3878782296321788
1114	22	-1.1473211844668825
1114	23	-0.6993351101231013
1114	24	-0.8643030390690001
1114	25	-0.621207542251355
1115	1	-0.6313645895257736
1115	2	-0.27078526271321834
1115	3	-0.3050490746779033
1115	4	-2.91195731401697
1115	5	-0.8374368690654386
1115	6	-0.42939658616028
1115	7	-0.546966953337467
1115	8	-0.30477609661594585
1115	9	-0.08362977327641374
1115	10	-0.4746257669917924
1115	11	-0.4648982878309482
1115	12	-0.5304402420098029
1115	13	-0.6577110932054779
1115	14	-5.8767273509427085
1115	15	-2.3367581290056663
1115	16	-1.1267860635087885
1115	17	-0.1686250850689035
1115	18	-0.02259205496257266
1115	19	-0.4071935651976375
1115	20	-0.4079320955024244
1115	21	-0.5414178573336486
1115	22	-1.5872475349281814
1115	23	-0.8582357433042489
1115	24	-0.5480077196612001
1115	25	-0.2239896127085501
1116	1	-0.6701594070650607
1116	2	-0.4138256738311874
1116	3	-0.5009649867538464
1116	4	-0.35443883645902474
1116	5	-0.885526906726458
1116	6	-0.7807383058298001
1116	7	-0.6595747310587186
1116	8	-0.5029484108598794
1116	9	-0.6140598567147819
1116	10	-0.3492219873066986
1116	11	-0.739352543879356
1116	12	-1.0919842952768164
1116	13	-1.0976110626930549
1116	14	-0.13157877108914562
1116	15	-0.640024551017305
1116	16	-0.667799904558315
1116	17	-0.2633172907915835
1116	18	-0.32627990423689823
1116	19	-0.6432881136727815
1116	20	-0.9327933186838635
1116	21	-1.0353506357048303
1116	22	-1.0408701985599436
1116	23	-1.618018409732251
1116	24	-1.3972655636129545
1116	25	-0.7776516203318025
1201	1	1.1400261231643398
1201	2	1.305300080037977
1201	3	1.285830421479865
1201	4	1.7797391512969867
1201	5	1.559981872806226
1201	6	1.3393368812935975
1201	7	1.2002804274257102
1201	8	1.2936087797946527
1201	9	1.4449898576726454
1201	10	1.923598025072529
1201	11	1.3917494249095412
1201	12	1.3154101827258842
1201	13	1.244018167092051
1201	14	2.1929545955280716
1201	15	1.8403856087902142
1201	16	2.02581070264754
1201	17	2.38736788720108
1201	18	1.7684741617161563
1201	19	1.683611548899684
1201	20	1.6368391428793672
1201	21	1.6198815355868703
1201	22	1.8855074532397964
1201	23	2.0854423240046103
1201	24	1.7334239024190765
1201	25	2.1276392801074238
1202	1	0.7731791412937845
1202	2	0.792648741355201
1202	3	0.7698583734778488
1202	4	1.1889546274240175
1202	5	0.9731840037016752
1202	6	0.6693739051633187
1202	7	0.6587945525860803
1202	8	0.758243721124515
1202	9	1.0046358057475828
1202	10	1.146588766017878
1202	11	0.825096222846895
1202	12	0.7732294400776851
1202	13	0.6978366334660677
1202	14	1.5842203354660143
1202	15	1.1826271371386738
1202	16	1.240987694488183
1202	17	1.701990187185089
1202	18	1.8799293171104665
1202	19	1.3364859602159314
1202	20	1.155298838759137
1202	21	1.064024091621505
1202	22	1.1754367201977232
1202	23	1.477682974933181
1202	24	1.197547421691766
1202	25	1.5717085553325916
1203	1	0.7866364445439693
1203	2	0.9683402608455793
1203	3	0.8141081688298506
1203	4	1.1887482012828698
1203	5	1.0321556846861526
1203	6	0.7272980987596753
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2211	18	-0.8027514569183012
2211	19	-0.671571149564862
2211	20	-0.7698053948027805
2211	21	-0.7284147153732476
2211	22	-0.4884338788638374
2211	23	-0.05904974425563836
2211	24	-0.421365110694758
2211	25	-7.617102899102363
2212	1	-1.5696254490512587
2212	2	-4.000686190917502
2212	3	-1.6736922483138805
2212	4	-3.7964731745321814
2212	5	-1.3309922849907352
2212	6	-1.2528868440284953
2212	7	-1.199446697251183
2212	8	-1.1219530852957433
2212	9	-1.2481675344438514
2212	10	-0.619994475961974
2212	11	-1.0074888940592004
2212	12	-1.111894142625296
2212	13	-1.3024870072732426
2212	14	-6.532202576594958
2212	15	-5.248980943659575
2212	16	-3.003136127295271
2212	17	-7.87943374689927
2212	18	-3.32803715748069
2212	19	-2.1497003747230323
2212	20	-2.02551344778702
2212	21	-1.6764387724287033
2212	22	-1.5603983702452666
2212	23	-0.8899265184473961
2212	24	-1.1721054340727477
2212	25	-1.0390551303514144
2213	1	-0.3337946172405992
2213	2	-0.3133137985058987
2213	3	-0.36223035112288215
2213	4	0.0430400677939744
2213	5	-0.04301955564414328
2213	6	-0.37363718038978533
2213	7	-0.4085221343284431
2213	8	-0.4843631617884938
2213	9	-0.2905803076708082
2213	10	-4.137739150963096
2213	11	-1.6413384529215165
2213	12	-1.1995025848002001
2213	13	-0.8543746606419802
2213	14	-0.1337419243629938
2213	15	-0.5713316891490834
2213	16	-0.37784770180819294
2213	17	0.023998011293755894
2213	18	0.11860203394945046
2213	19	-0.34053772138457816
2213	20	-0.8488324591146608
2213	21	-0.7259752997434319
2213	22	-0.955089866061158
2213	23	-1.165324227947977
2213	24	-0.6992142050368457
2213	25	-0.3206759958904968
2214	1	-1.0132865796758992
2214	2	-0.719848455195244
2214	3	-0.7440421881664285
2214	4	-0.3202363626469946
2214	5	-3.5279800989021943
2214	6	-1.4941486977978435
2214	7	-1.2456345100000341
2214	8	-0.8680136558249316
2214	9	-0.713477565155948
2214	10	-4.211500021018967
2214	11	-2.0287857495409156
2214	12	-1.3509136438122624
2214	13	-1.049819503762187
2214	14	-0.4503314347036138
2214	15	-0.6818884088838288
2214	16	-0.6392798082824598
2214	17	-0.23475713134523346
2214	18	0.004957127926537908
2214	19	-0.7032257228916734
2214	20	-1.0517914579651233
2214	21	-1.1049020112376553
2214	22	-0.7445419437617247
2214	23	-0.4679386035162878
2214	24	-1.080952252630901
2214	25	-0.6830050424714145
2215	1	-2.5635411763678917
2215	2	-5.176055941985247
2215	3	-3.276883808182564
2215	4	-2.3191145380302736
2215	5	-2.3775635517040214
2215	6	-2.455405185332065
2215	7	-2.602840407647257
2215	8	-2.8618659112962206
2215	9	-8.711804960441441
2215	10	-4.499520986657792
2215	11	-3.626137746108469
2215	12	-4.024927800330804
2215	13	-3.315304166205703
2215	14	-8.430883154520995
2215	15	-5.441540215295543
2215	16	-9.84026013915443
2215	17	-5.149592226355146
2215	18	-3.624540010805891
2215	19	-4.2105079315404765
2215	20	-4.140371364424553
2215	21	-4.078795597884955
2215	22	-10.326317881911692
2215	23	-11.511768485692762
2215	24	-7.603469115720486
2215	25	-5.909985724776533
2216	1	-1.9200513537014174
2216	2	-1.85255083357967
2216	3	-1.6779967615831701
2216	4	-7.18681981382101
2216	5	-3.568453251200289
2216	6	-2.6631313053124828
2216	7	-2.1607023622637733
2216	8	-8.04961874973796
2216	9	-3.9321967048790434
2216	10	-2.188124520695407
2216	11	-2.119142499215357
2216	12	-2.2897614415896648
2216	13	-2.0413982492982257
2216	14	-1.3520940756131026
2216	15	-1.979383283812922
2216	16	-1.7293557855611752
2216	17	-1.4308541841298705
2216	18	-7.544275605417983
2216	19	-4.718014176901853
2216	20	-4.116491439589964
2216	21	-3.1448200740167778
2216	22	-2.6739491292469495
2216	23	-1.9906254029634383
2216	24	-2.343047052461529
2216	25	-1.7991069526887955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104615.0
3	2.0
4	850.0
5	5055.0
6	6155.0
7	5600.0
8	5235.0
9	4959.0
10	4756.0
11	4453.0
12	4454.0
13	4366.0
14	4322.0
15	4409.0
16	4633.0
17	5062.0
18	5459.0
19	6770.0
20	8775.0
21	11040.0
22	13914.0
23	16602.0
24	19386.0
25	22110.0
26	24854.0
27	28334.0
28	31267.0
29	37508.0
30	45336.0
31	53963.0
32	69390.0
33	90486.0
34	118069.0
35	156270.0
36	222038.0
37	320270.0
38	859094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.344936495249776	17.890148670035412	12.0967939570633	25.66812087765151
2	15.136538041130104	19.80946298824141	40.21176134417329	24.84223762645519
3	18.698864761117854	24.395199118662156	29.221857867059136	27.684078253160855
4	11.870580691289092	16.282418325935566	38.23052140565766	33.61647957711768
5	13.41302672363601	37.33705191832596	35.617686889233816	13.632234468804208
6	32.72684655926114	36.211394475980576	17.889249158698682	13.172509806059601
7	28.71659083211092	31.132778944738437	21.888475766212345	18.262154456938294
8	25.992361349303213	35.211629774854266	20.477714019804512	18.318294856038005
9	26.748360646940768	14.86598624123979	19.53516425727379	38.85048885454565
10	14.216253832023328	28.280879529042668	34.97994962614563	22.522917012788366
11	35.154685423960196	22.269377802486044	23.46573481210867	19.11020196144509
12	23.247485951251303	24.43990690409757	30.93519651074978	21.37741063390135
13	29.018987762945546	21.11674337991368	25.437256921472567	24.4270119356682
14	21.3646922856651	20.486268974153894	28.22189794559451	29.9271407945865
15	24.209582566160105	28.443176509384706	23.1847192832999	24.162521641155294
16	23.48281029918146	27.21713719778019	26.110752648011985	23.18929985502636
17	22.581492860317276	27.16802246568637	27.294606646528273	22.95587802746808
18	22.55609495558644	26.885993102148397	28.009716928760124	22.548195013505037
19	23.905960540692544	26.66347534705601	26.87199874636952	22.55856536588193
20	24.191122645904038	26.46724068719316	26.721535644442156	22.620101022460645
21	24.29425178387954	26.104901863547113	26.37475403093017	23.226092321643176
22	23.986709308636716	26.288920530665806	26.428045110715686	23.296325049981796
23	23.472189366387326	26.11859270075249	26.74653389427009	23.66268403859009
24	23.555641821501634	26.41876467546923	26.592079068465196	23.43351443456394
25	23.676281854487076	26.05014450476151	26.618760329052648	23.654813311698767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	262.0
2	353.5
3	445.0
4	445.0
5	445.0
6	885.0
7	1325.0
8	1325.0
9	1325.0
10	2012.5
11	2700.0
12	2700.0
13	2700.0
14	5141.5
15	7583.0
16	7583.0
17	7583.0
18	14477.0
19	21371.0
20	21371.0
21	21371.0
22	34030.5
23	46690.0
24	46690.0
25	46690.0
26	66860.5
27	87031.0
28	87031.0
29	87031.0
30	112805.0
31	138579.0
32	138579.0
33	138579.0
34	164998.5
35	191418.0
36	191418.0
37	191418.0
38	216570.5
39	241723.0
40	241723.0
41	241723.0
42	262672.0
43	283621.0
44	283621.0
45	283621.0
46	299966.0
47	316311.0
48	316311.0
49	316311.0
50	311694.0
51	307077.0
52	307077.0
53	307077.0
54	280912.0
55	254747.0
56	254747.0
57	254747.0
58	221607.0
59	188467.0
60	188467.0
61	188467.0
62	154513.0
63	120559.0
64	120559.0
65	120559.0
66	93902.5
67	67246.0
68	67246.0
69	67246.0
70	49440.0
71	31634.0
72	31634.0
73	31634.0
74	22446.0
75	13258.0
76	13258.0
77	13258.0
78	9196.0
79	5134.0
80	5134.0
81	5134.0
82	3478.0
83	1822.0
84	1822.0
85	1822.0
86	1225.0
87	628.0
88	628.0
89	628.0
90	404.5
91	181.0
92	181.0
93	181.0
94	106.5
95	32.0
96	32.0
97	32.0
98	24.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17048227340601005
2	0.26237616750527176
3	0.26271953562894956
4	0.46861164678922906
5	0.25915709134579273
6	0.018627720709518722
7	0.019228614925954807
8	0.01682503806021046
9	0.01871356274043816
10	0.020516245389746425
11	0.019571983049632573
12	0.027898660048818365
13	0.02678271364686563
14	0.024636662873879604
15	0.04141877991863034
16	0.020258719296988106
17	0.04189091108868726
18	0.03133234128559601
19	0.026868555677785073
20	0.0290146064507711
21	0.034680180491454216
22	0.043478988660696924
23	0.028413712234335007
24	0.04914456270138005
25	0.03742712548087632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2329861.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.51120208175018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.81448367014586	60.87792244371894
2	11.540205031262266	16.96668688235901
3	2.996377094212039	6.608018462572457
4	1.1001459860065077	3.2349221558700276
5	0.5032338494954254	1.8496662602317637
6	0.2928131061497029	1.2915026051013467
7	0.1694645676379936	0.872028085413308
8	0.11609695212780294	0.6827541207153759
9	0.08592635582843026	0.5684894736706899
>10	0.3587873813708638	4.435442846595952
>50	0.013956092920278714	0.6956411923643894
>100	0.006917393574934399	0.8844290194558855
>500	0.0012386260972444224	0.570251263420578
>1k	3.538931706384528E-4	0.46224518851025037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2778	0.11923458094710371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.292101545972056E-5
10	0.0	0.0	0.0	0.0	4.292101545972056E-5
11	0.0	0.0	0.0	0.0	4.292101545972056E-5
12	0.0	0.0	0.0	0.0	4.292101545972056E-5
13	0.0	0.0	0.0	4.292101545972056E-5	4.292101545972056E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCT	70	1.0578028E-4	12.2552	4
GTCCTAC	530	0.0	10.454501	1
CGCTATT	75	0.0026757806	10.120785	11
GTATTAC	200	2.1827873E-11	10.030914	1
GTATTGG	315	0.0	10.008168	1
TAGAACT	410	0.0	9.99679	4
GTTACAC	220	1.8189894E-12	9.965302	3
TAGACTG	240	0.0	9.908254	5
CGCAGAT	155	4.1496605E-8	9.793466	8
TAGGACG	370	0.0	9.78944	4
GTAGGAC	450	0.0	9.743851	3
GCTACAC	305	0.0	9.688291	3
TAAGGCT	305	0.0	9.688083	4
TAGAACA	505	0.0	9.626197	4
GTGTTAC	220	1.4551915E-11	9.553251	1
GTTAGAC	130	4.062211E-6	9.532029	3
TTAGGAC	350	0.0	9.532028	3
CCTATAC	220	1.6370905E-11	9.532028	3
AAGACCG	80	0.0044703763	9.511924	5
TCGAACC	80	0.0045640874	9.488031	9
>>END_MODULE