FastQCFastQC Report
Thu 26 May 2016
SRR1513148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329861
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31380.13468614651260313No Hit
GTACATGGGAAGCAGTGGTATCAAC31370.1346432254971434No Hit
CCCATGTACTCTGCGTTGATACCAC29120.12498599701870627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG550.003022668712.1156145
TAGGACC3650.011.9970664
CTAGGAC3300.011.5401713
TAGGACA4300.011.5118474
CGTATAG600.0059300911.06998710
GTAGGAC5700.011.02393
TTGCGAG951.3772176E-510.98816911
TTAGGAC3150.010.8807333
AAGGCGT2200.010.8165816
AAGACCG1252.1792948E-710.6617395
ATTATAC1409.3910785E-810.2006863
ATTGCGA750.002675020610.12113110
TAGTACC1701.5061232E-910.0793754
TAGGACG3700.010.0339744
GTGCGCC1804.0199666E-1010.01699311
ACCGTAT1054.157396E-59.9412518
CGTGCGC1356.5528E-79.8399910
AGGCGTG1951.6552804E-109.7349917
GTATAGC3250.09.6731531
CGTAGAC800.0044367199.520643
Produced by FastQC (version 0.11.5)