##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513148_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2329861 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86795349593817 31.0 31.0 34.0 28.0 34.0 2 30.964059658494648 33.0 31.0 34.0 27.0 34.0 3 31.063361290652104 33.0 31.0 34.0 27.0 34.0 4 34.47488541161898 37.0 35.0 37.0 31.0 37.0 5 34.238677328819186 37.0 35.0 37.0 30.0 37.0 6 34.16262429389565 37.0 35.0 37.0 30.0 37.0 7 34.03301742035254 37.0 35.0 37.0 30.0 37.0 8 34.00404530570708 37.0 35.0 37.0 30.0 37.0 9 35.57617471600237 39.0 37.0 39.0 30.0 39.0 10 35.41697723598103 39.0 35.0 39.0 30.0 39.0 11 35.44292470666705 39.0 35.0 39.0 30.0 39.0 12 35.376497997090816 39.0 35.0 39.0 30.0 39.0 13 35.35215491396268 39.0 35.0 39.0 30.0 39.0 14 36.52818215335593 40.0 37.0 41.0 29.0 41.0 15 36.5021449777476 40.0 37.0 41.0 29.0 41.0 16 36.46349589095659 40.0 37.0 41.0 29.0 41.0 17 36.38804932998149 40.0 37.0 41.0 29.0 41.0 18 36.33942883287887 40.0 36.0 41.0 29.0 41.0 19 36.41950528379161 40.0 37.0 41.0 29.0 41.0 20 36.381554092711966 40.0 37.0 41.0 29.0 41.0 21 36.328574108069105 40.0 37.0 41.0 29.0 41.0 22 36.27137155392532 40.0 37.0 41.0 29.0 41.0 23 36.19469659348776 40.0 37.0 41.0 28.0 41.0 24 36.13043138625008 40.0 37.0 41.0 28.0 41.0 25 36.075844009578255 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39691139797262665 1101 2 0.4499415972702181 1101 3 0.39451233301157274 1101 4 0.5320713474647718 1101 5 0.48171271481943023 1101 6 0.4810387219392851 1101 7 0.5482247863023488 1101 8 0.4894838823763479 1101 9 0.5668431323628909 1101 10 0.6048100268443264 1101 11 0.5422066304479856 1101 12 0.5681818184388305 1101 13 0.6637953721524497 1101 14 0.8113947545297435 1101 15 0.8098577596692564 1101 16 0.7231066845713556 1101 17 0.7740761155981914 1101 18 0.7224376642923573 1101 19 0.7898146721262336 1101 20 0.7187884667550009 1101 21 0.7798084790357152 1101 22 0.7804901059008742 1101 23 0.7470695946642394 1101 24 0.7308301386999076 1101 25 0.6473571527617281 1102 1 0.4014487037537684 1102 2 0.41183884768505763 1102 3 0.3487397634717482 1102 4 0.43662511853088404 1102 5 0.4567326212063634 1102 6 0.4114401385409394 1102 7 0.4435683986818617 1102 8 0.4841634061127351 1102 9 0.4771313184664123 1102 10 0.530107960010703 1102 11 0.6347207301588327 1102 12 0.520174902180365 1102 13 0.6382279635861536 1102 14 0.7786758092272876 1102 15 0.6719690084617227 1102 16 0.6844178782055366 1102 17 0.7541689737661628 1102 18 0.7633736507369733 1102 19 0.768586784613909 1102 20 0.7767119636560835 1102 21 0.7051999363627388 1102 22 0.6390897950651464 1102 23 0.7339471064484968 1102 24 0.7057046842165278 1102 25 0.7374041438294583 1103 1 0.5097707187534937 1103 2 0.5572067271204411 1103 3 0.5759295274891088 1103 4 0.651508734753456 1103 5 0.5920939251572079 1103 6 0.512062621574934 1103 7 0.5770078779107095 1103 8 0.6960361252902842 1103 9 0.7847956043900552 1103 10 0.7122519193620747 1103 11 0.7828734820553507 1103 12 0.7542446640565288 1103 13 0.757051788465624 1103 14 0.831684362283255 1103 15 0.8268222029083034 1103 16 0.8994459925275109 1103 17 0.9852345708951162 1103 18 0.9146415681934172 1103 19 0.8524373896709676 1103 20 0.8774943798137258 1103 21 0.7400742242477776 1103 22 0.8224789011071323 1103 23 0.7349103997440878 1103 24 0.8723176430195423 1103 25 0.8576834586556004 1104 1 0.3443965842410819 1104 2 0.4002866190981287 1104 3 0.3403921115909725 1104 4 0.5022250636260921 1104 5 0.4310817094355741 1104 6 0.3451965328651596 1104 7 0.3104912059832188 1104 8 0.40973996765143994 1104 9 0.37705729504853736 1104 10 0.4437482045657717 1104 11 0.48659366152703143 1104 12 0.5054517587505956 1104 13 0.48757208832460464 1104 14 0.6017363098903132 1104 15 0.5382914580649327 1104 16 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1107 15 0.22371468336528721 1107 16 0.20181344335048834 1107 17 0.18340383798076942 1107 18 0.3392654199580676 1107 19 0.2976603844297827 1107 20 0.3125539112514417 1107 21 0.257033382920973 1107 22 0.31573253247726285 1107 23 0.3641383773812521 1107 24 0.2735177773609081 1107 25 0.4742909905502799 1108 1 0.19660514312381494 1108 2 0.23504734217200252 1108 3 0.2485203945432488 1108 4 0.32603008303798475 1108 5 0.19803977509823056 1108 6 0.13219285266913516 1108 7 0.1343908177454054 1108 8 0.18840493983936568 1108 9 0.24128068703875982 1108 10 0.3584495727450303 1108 11 0.24261728474299815 1108 12 0.20349243747277512 1108 13 0.24891526760639238 1108 14 0.26202172365361776 1108 15 0.27441626861948265 1108 16 0.3291172592674485 1108 17 0.27586128435341095 1108 18 0.38781021711373853 1108 19 0.2862330092320846 1108 20 0.428270712218648 1108 21 0.3757254599371649 1108 22 0.28853133425116795 1108 23 0.24757454204485896 1108 24 0.3653620655331551 1108 25 0.37387656418113124 1109 1 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-1.3719228318281438 2215 18 -1.4938796356077617 2215 19 -1.423160332983457 2215 20 -1.3826342299704208 2215 21 -1.3968768162236955 2215 22 -1.4315663739885807 2215 23 -1.3938955632576224 2215 24 -1.347897371987763 2215 25 -1.4922896208317482 2216 1 -0.25393338208888494 2216 2 -0.20079703798552728 2216 3 -0.2585283753295151 2216 4 -0.027720546319514483 2216 5 -0.3063676560137907 2216 6 -0.3531406854720629 2216 7 -0.4967784132863997 2216 8 -0.40417767026619345 2216 9 -0.5049162869250452 2216 10 -0.5182958254782548 2216 11 -0.4240491771158048 2216 12 -0.519242361943526 2216 13 -0.6695322887797701 2216 14 -0.7560570079030029 2216 15 -0.7335417457682709 2216 16 -0.7062300522026419 2216 17 -0.7298092309943769 2216 18 -0.7745779791263274 2216 19 -0.8389314258822225 2216 20 -0.754397490507003 2216 21 -0.9679097089866531 2216 22 -0.8856432132514556 2216 23 -0.9102253436040826 2216 24 -0.920208708384564 2216 25 -0.8675032264028317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49478.0 3 1.0 4 816.0 5 6646.0 6 7663.0 7 5922.0 8 4797.0 9 4215.0 10 3845.0 11 3656.0 12 3335.0 13 3111.0 14 3162.0 15 3160.0 16 3269.0 17 3412.0 18 3821.0 19 4646.0 20 5852.0 21 7901.0 22 9768.0 23 11830.0 24 13842.0 25 15561.0 26 17319.0 27 19544.0 28 22012.0 29 26547.0 30 33830.0 31 43088.0 32 55437.0 33 72316.0 34 96284.0 35 129276.0 36 184199.0 37 301058.0 38 1149242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58198730708319 18.755700266904718 13.096004427341674 27.56630799867042 2 14.540274733718109 20.272750608024985 38.44902763219232 26.73794702606459 3 17.321023402832445 24.221386451064035 29.613369515108694 28.844220630994826 4 11.785444796691227 16.79869482695126 36.705974660551135 34.70988571580638 5 13.449392100397937 37.547035759204405 35.3382021429406 13.665369997457061 6 32.91849182575623 36.55586235644905 17.450903722893912 13.07474209490081 7 28.401563822899544 31.20171014149283 21.951266634723876 18.445459400883752 8 26.087232747437472 35.51056046306177 20.061703221840553 18.340503567660196 9 26.54979540661781 14.651358540405528 19.145373545494074 39.65347250748258 10 14.640451786892362 28.61096648554729 34.240532653209264 22.50804907435108 11 35.26107351468607 22.110846956105963 23.379506331064384 19.248573198143582 12 22.95991048393016 24.618593126371056 30.931115633078537 21.490380756620244 13 28.95756442122513 21.005845413095457 25.459587503288823 24.57700266239059 14 21.837505182799372 20.672394715132743 27.056873872767962 30.43322622929992 15 24.12839220880559 28.67029406475322 23.02849826663479 24.1728154598064 16 23.731587420880473 27.338025744883492 25.539291828997523 23.391095005238512 17 22.905531815516284 27.401500301794712 26.57029842045026 23.12266946223874 18 22.884359103820408 27.089966580455922 27.26921452049995 22.75645979522372 19 23.896876251415858 26.682364312720804 26.788808431060907 22.631951004802435 20 24.109034830833256 26.42642629753449 26.748419755513314 22.716119116118943 21 24.10468177670417 26.073999867802193 26.466086541490796 23.35523181400284 22 23.823446599274806 26.435324758268326 26.276602502472258 23.464626139984617 23 23.49808002513609 26.186302376510806 26.367568950405158 23.94804864794795 24 23.385729878306044 26.410202153690715 26.701635848662214 23.502432119341023 25 23.617478340926795 26.114414845559057 26.488214931830523 23.779891881683625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 162.0 2 247.5 3 333.0 4 333.0 5 333.0 6 850.5 7 1368.0 8 1368.0 9 1368.0 10 2010.5 11 2653.0 12 2653.0 13 2653.0 14 4854.5 15 7056.0 16 7056.0 17 7056.0 18 13974.5 19 20893.0 20 20893.0 21 20893.0 22 33477.0 23 46061.0 24 46061.0 25 46061.0 26 65887.0 27 85713.0 28 85713.0 29 85713.0 30 110704.5 31 135696.0 32 135696.0 33 135696.0 34 161498.5 35 187301.0 36 187301.0 37 187301.0 38 212380.5 39 237460.0 40 237460.0 41 237460.0 42 258313.5 43 279167.0 44 279167.0 45 279167.0 46 297923.0 47 316679.0 48 316679.0 49 316679.0 50 313538.0 51 310397.0 52 310397.0 53 310397.0 54 285052.0 55 259707.0 56 259707.0 57 259707.0 58 226018.5 59 192330.0 60 192330.0 61 192330.0 62 158370.5 63 124411.0 64 124411.0 65 124411.0 66 96579.0 67 68747.0 68 68747.0 69 68747.0 70 50599.0 71 32451.0 72 32451.0 73 32451.0 74 22865.0 75 13279.0 76 13279.0 77 13279.0 78 9307.5 79 5336.0 80 5336.0 81 5336.0 82 3591.5 83 1847.0 84 1847.0 85 1847.0 86 1242.5 87 638.0 88 638.0 89 638.0 90 396.5 91 155.0 92 155.0 93 155.0 94 87.5 95 20.0 96 20.0 97 20.0 98 10.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18507541866231506 2 0.008026229890967745 3 0.00613770521074004 4 0.0023606558502846306 5 0.07918927352318443 6 0.3300196878697914 7 0.02991594777542523 8 0.01386348799348974 9 0.005064679824247026 10 0.0038628913913748503 11 0.0 12 0.0 13 0.0 14 0.0016739196029291019 15 0.0 16 0.0 17 0.019872430157850618 18 0.02940089558990858 19 0.0 20 0.0 21 8.154992937346906E-4 22 2.1460507729860278E-4 23 0.00613770521074004 24 0.0 25 2.575260927583233E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2329861.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.40696059485602 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82247435674724 56.90464767214264 2 12.631151575079217 17.78641982428512 3 3.405466878910768 7.193057170516733 4 1.27316741106219 3.585593909652419 5 0.6157661973601329 2.167711319658961 6 0.3350167043118253 1.415250473946073 7 0.2025047561300576 0.9980421069583927 8 0.14741362510620148 0.8303156235197765 9 0.1098409343532276 0.6960209703038877 >10 0.42842838717627185 5.091312031144154 >50 0.018196335088301048 0.882083501114817 >100 0.008681761085409145 1.0787099862124105 >500 0.0014030575661974024 0.6587672252080878 >1k 4.88020023023326E-4 0.712068185336533 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3138 0.13468614651260313 No Hit GTACATGGGAAGCAGTGGTATCAAC 3137 0.1346432254971434 No Hit CCCATGTACTCTGCGTTGATACCAC 2912 0.12498599701870627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.292101545972056E-5 0.0 6 0.0 0.0 0.0 4.292101545972056E-5 0.0 7 0.0 0.0 0.0 8.584203091944112E-5 0.0 8 0.0 0.0 0.0 8.584203091944112E-5 0.0 9 0.0 0.0 0.0 8.584203091944112E-5 0.0 10 0.0 0.0 0.0 8.584203091944112E-5 0.0 11 0.0 0.0 0.0 8.584203091944112E-5 0.0 12 0.0 4.292101545972056E-5 0.0 8.584203091944112E-5 0.0 13 0.0 4.292101545972056E-5 0.0 8.584203091944112E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 55 0.0030226687 12.115614 5 TAGGACC 365 0.0 11.997066 4 CTAGGAC 330 0.0 11.540171 3 TAGGACA 430 0.0 11.511847 4 CGTATAG 60 0.00593009 11.069987 10 GTAGGAC 570 0.0 11.0239 3 TTGCGAG 95 1.3772176E-5 10.988169 11 TTAGGAC 315 0.0 10.880733 3 AAGGCGT 220 0.0 10.816581 6 AAGACCG 125 2.1792948E-7 10.661739 5 ATTATAC 140 9.3910785E-8 10.200686 3 ATTGCGA 75 0.0026750206 10.121131 10 TAGTACC 170 1.5061232E-9 10.079375 4 TAGGACG 370 0.0 10.033974 4 GTGCGCC 180 4.0199666E-10 10.016993 11 ACCGTAT 105 4.157396E-5 9.941251 8 CGTGCGC 135 6.5528E-7 9.83999 10 AGGCGTG 195 1.6552804E-10 9.734991 7 GTATAGC 325 0.0 9.673153 1 CGTAGAC 80 0.004436719 9.52064 3 >>END_MODULE