##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4997 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 10.057834700820493 2.0 2.0 23.0 2.0 30.0 2 10.074644786872124 2.0 2.0 22.0 2.0 30.0 3 10.20292175305183 2.0 2.0 23.0 2.0 30.0 4 11.449869921953171 2.0 2.0 27.0 2.0 35.0 5 11.183910346207725 2.0 2.0 26.0 2.0 35.0 6 11.01921152691615 2.0 2.0 27.0 2.0 35.0 7 10.610366219731839 2.0 2.0 25.0 2.0 35.0 8 10.291174704822893 2.0 2.0 21.0 2.0 35.0 9 10.349209525715429 2.0 2.0 19.0 2.0 35.0 10 9.92055233139884 2.0 2.0 17.0 2.0 35.0 11 9.865719431658995 2.0 2.0 17.0 2.0 35.0 12 9.745247148288973 2.0 2.0 11.0 2.0 35.0 13 9.550730438262958 2.0 2.0 10.0 2.0 35.0 14 9.499299579747849 2.0 2.0 10.0 2.0 36.0 15 9.427256353812288 2.0 2.0 8.0 2.0 36.0 16 9.264158495097059 2.0 2.0 2.0 2.0 36.0 17 9.151691014608765 2.0 2.0 2.0 2.0 36.0 18 8.993396037622574 2.0 2.0 2.0 2.0 36.0 19 9.010006003602161 2.0 2.0 2.0 2.0 36.0 20 9.004202521512907 2.0 2.0 2.0 2.0 36.0 21 8.881128677206323 2.0 2.0 2.0 2.0 36.0 22 8.701220732439463 2.0 2.0 2.0 2.0 36.0 23 8.497898739243546 2.0 2.0 2.0 2.0 36.0 24 8.506303782269361 2.0 2.0 2.0 2.0 36.0 25 8.281769061436862 2.0 2.0 2.0 2.0 36.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.987522546897546 1101 2 3.0168718434343447 1101 3 0.25838970057720445 1101 4 5.382229662698414 1101 5 1.54074393488456 1101 6 5.446825960497836 1101 7 4.951661142676768 1101 8 3.8946118551587325 1101 9 4.051612666847044 1101 10 2.5733196924603163 1101 11 2.7298464556277047 1101 12 3.7660392992424256 1101 13 3.0788008432539726 1101 14 4.176238388347764 1101 15 4.080203260281385 1101 16 -1.0663352272727291 1101 17 -1.0030607413419919 1101 18 -2.6888094110750336 1101 19 -1.149297100468977 1101 20 -1.8375693317099602 1101 21 -0.8836608270201989 1101 22 -0.6521960678210696 1101 23 -1.6144643984487743 1101 24 -0.6803379779942285 1101 25 -1.2500264926046158 1102 1 8.62085588023088 1102 2 7.650205176767679 1102 3 5.12505636724387 1102 4 6.4822296626984155 1102 5 9.640743934884561 1102 6 11.08015929383117 1102 7 10.984994476010101 1102 8 6.061278521825399 1102 9 6.818279333513711 1102 10 7.373319692460317 1102 11 7.363179788961039 1102 12 7.532705965909093 1102 13 6.378800843253973 1102 14 7.642905055014431 1102 15 7.94686992694805 1102 16 8.13366477272727 1102 17 7.396939258658007 1102 18 8.544523922258298 1102 19 8.617369566197691 1102 20 8.429097334956708 1102 21 8.149672506313134 1102 22 8.581137265512263 1102 23 8.585535601551225 1102 24 8.886328688672437 1102 25 9.016640174062053 1103 1 3.4208558802308797 1103 2 3.050205176767678 1103 3 3.52505636724387 1103 4 -0.5177703373015863 1103 5 2.04074393488456 1103 6 -0.1198407061688318 1103 7 3.3849944760101014 1103 8 4.261278521825398 1103 9 1.2182793335137099 1103 10 1.5733196924603163 1103 11 2.963179788961039 1103 12 4.332705965909092 1103 13 5.978800843253973 1103 14 3.8429050550144304 1103 15 6.146869926948051 1103 16 5.933664772727271 1103 17 4.3969392586580085 1103 18 5.5445239222583 1103 19 2.2173695661976893 1103 20 5.029097334956706 1103 21 6.349672506313135 1103 22 0.981137265512265 1103 23 0.785535601551226 1103 24 1.0863286886724381 1103 25 1.2166401740620518 1104 1 2.1065701659451666 1104 2 4.42163374819625 1104 3 3.4964849386724417 1104 4 3.1679439484127005 1104 5 1.8978867920274176 1104 6 6.137302150974026 1104 7 4.784994476010102 1104 8 4.63270709325397 1104 9 3.21827933351371 1104 10 1.5733196924603163 1104 11 3.706036931818181 1104 12 2.7898488230519494 1104 13 4.721657986111115 1104 14 3.700047912157288 1104 15 3.6040127840909086 1104 16 0.7908076298701285 1104 17 4.568367830086579 1104 18 4.573095350829728 1104 19 4.588798137626261 1104 20 5.54338304924242 1104 21 6.949672506313135 1104 22 6.466851551226551 1104 23 3.3855356015512257 1104 24 6.05775726010101 1104 25 5.416640174062051 1105 1 2.5351415945165936 1105 2 1.7073480339105362 1105 3 3.067913510101013 1105 4 5.167943948412699 1105 5 5.326458220598845 1105 6 4.994445008116882 1105 7 5.927851618867244 1105 8 5.061278521825399 1105 9 3.6468507620851387 1105 10 6.430462549603174 1105 11 6.991751217532466 1105 12 4.3612773944805205 1105 13 7.150229414682546 1105 14 7.414333626443003 1105 15 8.032584212662336 1105 16 5.7908076298701285 1105 17 8.425510687229435 1105 18 7.858809636544015 1105 19 6.01736956619769 1105 20 6.829097334956707 1105 21 7.66395822059885 1105 22 5.323994408369407 1105 23 8.099821315836941 1105 24 6.772042974386725 1105 25 8.130925888347766 1106 1 -4.036286976911977 1106 2 -6.149794823232321 1106 3 -4.360657918470415 1106 4 -4.4034846230158715 1106 5 -4.244970350829726 1106 6 -4.005554991883117 1106 7 -3.500719809704184 1106 8 -3.224435763888887 1106 9 -3.78172066648629 1106 10 -2.4266803075396837 1106 11 -2.722534496753247 1106 12 -2.0672940340909083 1106 13 -4.706913442460313 1106 14 -5.014237802128426 1106 15 -3.253130073051949 1106 16 -2.2091923701298724 1106 17 -2.4316321699134207 1106 18 -1.9983332205988438 1106 19 -3.8397732909451667 1106 20 -3.599474093614722 1106 21 -3.478898922258294 1106 22 -2.53314844877345 1106 23 -3.471607255591631 1106 24 -2.227957025613276 1106 25 -2.15478839736652 1107 1 0.9875225468975461 1107 2 3.3502051767676786 1107 3 4.7583897005772045 1107 4 2.04889632936508 1107 5 0.8740772682178939 1107 6 -1.8865073728354975 1107 7 1.9516611426767678 1107 8 2.7279451884920647 1107 9 -1.2817206664862901 1107 10 3.2399863591269824 1107 11 2.8965131222943725 1107 12 3.599372632575758 1107 13 -1.9211991567460274 1107 14 -1.8237616116522366 1107 15 -2.086463406385282 1107 16 -1.3996685606060622 1107 17 -1.1697274080086588 1107 18 -1.6888094110750336 1107 19 -1.315963767135643 1107 20 -1.0042359983766271 1107 21 -2.383660827020198 1107 22 -1.1521960678210688 1107 23 -2.9477977317821074 1107 24 -0.8470046446608945 1107 25 -0.4166931592712828 1108 1 -1.0362869769119776 1108 2 -1.2926519660894638 1108 3 -0.07494363275612947 1108 4 -0.4034846230158724 1108 5 0.04074393488455996 1108 6 -2.8626978490259747 1108 7 0.07070876172438822 1108 8 0.7755642361111121 1108 9 1.5039936192279963 1108 10 0.5733196924603163 1108 11 -0.15110592532467493 1108 12 -1.2101511769480515 1108 13 1.5788008432539726 1108 14 2.700047912157288 1108 15 -0.9674157873376625 1108 16 1.0765219155844132 1108 17 0.5683678300865793 1108 18 2.001666779401157 1108 19 2.7316552804834036 1108 20 2.2576687635281356 1108 21 1.5211010777417062 1108 22 -0.24743416305916455 1108 23 -0.47160725559163197 1108 24 2.629185831529581 1108 25 2.2737830312049088 1109 1 0.10657016594516655 1109 2 -0.5783662518037502 1109 3 0.06791351010101287 1109 4 0.025086805555556424 1109 5 0.4693153634559888 1109 6 0.9944450081168821 1109 7 1.4992801902958153 1109 8 1.7755642361111121 1109 9 2.5039936192279963 1109 10 2.859033978174603 1109 11 2.848894074675325 1109 12 3.218420251623378 1109 13 1.4359437003968303 1109 14 3.271476483585859 1109 15 3.4611556412337645 1109 16 3.7908076298701285 1109 17 3.7112249729437217 1109 18 3.001666779401157 1109 19 2.445940994769119 1109 20 2.1148116206709933 1109 21 0.6639582205988486 1109 22 2.895422979797978 1109 23 3.671249887265512 1109 24 3.772042974386725 1109 25 3.84521160263348 1110 1 -5.17914411976912 1110 2 -5.024794823232321 1110 3 -6.3249436327561295 1110 4 -6.367770337301586 1110 5 -5.45925606511544 1110 6 -8.719840706168831 1110 7 -8.215005523989898 1110 8 -7.938721478174601 1110 9 -7.78172066648629 1110 10 -7.426680307539684 1110 11 -7.436820211038961 1110 12 -7.067294034090908 1110 13 -7.421199156746027 1110 14 -7.15709494498557 1110 15 -7.253130073051949 1110 16 -7.066335227272729 1110 17 -7.003060741341992 1110 18 -6.8554760777417005 1110 19 -6.98263043380231 1110 20 -7.170902665043293 1110 21 -7.050327493686865 1110 22 -6.818862734487736 1110 23 -6.614464398448774 1110 24 -6.5136713113275615 1110 25 -6.583359825937949 1111 1 5.392284451659451 1111 2 6.278776605339106 1111 3 2.067913510101013 1111 4 6.453658234126987 1111 5 9.326458220598845 1111 6 5.2801592938311686 1111 7 1.4992801902958153 1111 8 2.0612785218253986 1111 9 2.6468507620851387 1111 10 3.1447482638888875 1111 11 2.9917512175324674 1111 12 3.3612773944805205 1111 13 3.150229414682544 1111 14 3.700047912157288 1111 15 3.8897270698051933 1111 16 4.076521915584413 1111 17 3.854082115800866 1111 18 4.287381065115442 1111 19 4.160226709054832 1111 20 3.400525906385278 1111 21 3.8068153634559927 1111 22 4.038280122655122 1111 23 4.528392744408368 1111 24 4.4863286886724385 1111 25 3.1309258883477646 1112 1 -4.17914411976912 1112 2 -4.149794823232321 1112 3 -7.2416102994227955 1112 4 -8.284437003968252 1112 5 -8.125922731782106 1112 6 -7.053174039502165 1112 7 -3.381672190656565 1112 8 -3.1053881448412683 1112 9 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12.727945188492066 1202 9 14.71827933351371 1202 10 14.406653025793648 1202 11 12.89651312229437 1202 12 13.932705965909092 1202 13 15.078800843253973 1202 14 13.009571721681098 1202 15 13.080203260281383 1202 16 16.766998106060605 1202 17 15.496939258658008 1202 18 16.97785725559163 1202 19 17.01736956619769 1202 20 17.16243066829004 1202 21 16.283005839646467 1202 22 14.514470598845596 1202 23 18.385535601551226 1202 24 16.986328688672437 1202 25 17.74997350739538 1203 1 3.19585588023088 1203 2 2.1002051767676786 1203 3 1.4250563672438705 1203 4 3.757229662698414 1203 5 1.79074393488456 1203 6 6.1551592938311686 1203 7 6.909994476010102 1203 8 2.8112785218253986 1203 9 -0.7817206664862901 1203 10 1.0733196924603163 1203 11 1.3131797889610386 1203 12 0.8077059659090917 1203 13 -0.04619915674602737 1203 14 -1.5320949449855696 1203 15 1.246869926948051 1203 16 1.4336647727272709 1203 17 1.2469392586580081 1203 18 -2.1054760777417005 1203 19 -0.73263043380231 1203 20 1.2040973349567068 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7.346869926948052 2213 16 8.533664772727272 2213 17 6.696939258658007 2213 18 6.0445239222583 2213 19 7.917369566197689 2213 20 7.329097334956707 2213 21 8.249672506313136 2213 22 6.281137265512264 2213 23 4.385535601551226 2213 24 8.08632868867244 2213 25 7.716640174062052 2214 1 -1.42914411976912 2214 2 -2.0247948232323214 2214 3 -2.6999436327561295 2214 4 0.5072296626984141 2214 5 -1.83425606511544 2214 6 0.5301592938311686 2214 7 2.5349944760101017 2214 8 1.0612785218253986 2214 9 2.09327933351371 2214 10 3.8233196924603163 2214 11 -0.6868202110389614 2214 12 -2.1922940340909083 2214 13 -5.796199156746027 2214 14 -0.9070949449855696 2214 15 -2.128130073051949 2214 16 0.058664772727270886 2214 17 -1.6280607413419919 2214 18 -0.35547607774170054 2214 19 -0.48263043380230997 2214 20 0.8290973349567068 2214 21 -2.800327493686865 2214 22 -3.0688627344877357 2214 23 -2.6144643984487743 2214 24 -3.7636713113275615 2214 25 -3.083359825937949 2215 1 -3.2700532106782108 2215 2 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-5.323761611652236 2216 15 -5.419796739718615 2216 16 -4.983001893939396 2216 17 -4.669727408008659 2216 18 -6.8554760777417005 2216 19 -6.98263043380231 2216 20 -7.170902665043293 2216 21 -7.050327493686865 2216 22 -6.818862734487736 2216 23 -6.614464398448774 2216 24 -6.5136713113275615 2216 25 -6.583359825937949 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 3133.0 3 0.0 4 23.0 5 103.0 6 96.0 7 86.0 8 74.0 9 64.0 10 46.0 11 51.0 12 37.0 13 31.0 14 35.0 15 27.0 16 27.0 17 30.0 18 27.0 19 35.0 20 44.0 21 32.0 22 37.0 23 41.0 24 33.0 25 39.0 26 37.0 27 38.0 28 32.0 29 47.0 30 47.0 31 61.0 32 47.0 33 73.0 34 81.0 35 85.0 36 89.0 37 115.0 38 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.02761656994196 17.65059035421253 11.326796077646588 24.99499699819892 2 13.783403656821378 21.016676712879246 40.6871609403255 24.51275868997388 3 18.020917135961383 24.7385358004827 28.801287208366855 28.439259855189057 4 11.28577186618299 17.251108424022572 35.73155985489722 35.73155985489722 5 13.067953357458787 37.29392842782469 35.36389223964616 14.274225975070365 6 32.41241241241241 37.33733733733734 17.077077077077078 13.173173173173172 7 26.881505204163332 32.986389111289036 21.537229783827062 18.594875900720574 8 25.740592473979184 35.58847077662129 19.65572457966373 19.015212169735786 9 26.621297037630104 13.971176941553242 20.11609287429944 39.291433146517214 10 13.751000800640512 29.303442754203363 34.087269815852686 22.858286629303443 11 35.61561561561562 22.1021021021021 22.56256256256256 19.71971971971972 12 23.143143143143146 24.904904904904903 31.37137137137137 20.58058058058058 13 28.9146976371646 22.807368842611133 24.809771726071286 23.468161794152984 14 21.641641641641645 20.84084084084084 27.44744744744745 30.07007007007007 15 24.60953143772527 30.13616339607529 21.46575891069283 23.788546255506606 16 22.62262262262262 27.60760760760761 27.147147147147148 22.62262262262262 17 22.3868642370845 27.392871445734883 26.57188626351622 23.6483780536644 18 22.3468161794153 26.73207849419303 27.793352022426916 23.127753303964756 19 23.608329995995195 27.53303964757709 26.491790148177813 22.3668402082499 20 23.82859431317581 27.11253504205046 25.991189427312776 23.067681217460954 21 24.60953143772527 26.21145374449339 26.371645975170203 22.807368842611133 22 23.11235730022031 26.857600640897257 26.75746044462247 23.272581614259963 23 23.583583583583582 26.526526526526528 26.286286286286288 23.603603603603602 24 23.177083333333336 27.303685897435898 27.063301282051285 22.45592948717949 25 24.50941129355226 26.57188626351622 26.231477773327995 22.687224669603523 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 2.0 2 1.5 3 1.0 4 1.0 5 1.0 6 2.0 7 3.0 8 3.0 9 3.0 10 7.0 11 11.0 12 11.0 13 11.0 14 13.0 15 15.0 16 15.0 17 15.0 18 25.0 19 35.0 20 35.0 21 35.0 22 69.0 23 103.0 24 103.0 25 103.0 26 141.0 27 179.0 28 179.0 29 179.0 30 252.0 31 325.0 32 325.0 33 325.0 34 386.0 35 447.0 36 447.0 37 447.0 38 500.0 39 553.0 40 553.0 41 553.0 42 580.5 43 608.0 44 608.0 45 608.0 46 620.0 47 632.0 48 632.0 49 632.0 50 632.5 51 633.0 52 633.0 53 633.0 54 573.5 55 514.0 56 514.0 57 514.0 58 453.0 59 392.0 60 392.0 61 392.0 62 332.0 63 272.0 64 272.0 65 272.0 66 207.0 67 142.0 68 142.0 69 142.0 70 106.0 71 70.0 72 70.0 73 70.0 74 53.5 75 37.0 76 37.0 77 37.0 78 27.0 79 17.0 80 17.0 81 17.0 82 10.5 83 4.0 84 4.0 85 4.0 86 3.0 87 2.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.40024014408645187 3 0.5003001801080648 4 0.7004202521512908 5 0.46027616569941965 6 0.040024014408645184 7 0.020012007204322592 8 0.020012007204322592 9 0.020012007204322592 10 0.020012007204322592 11 0.040024014408645184 12 0.040024014408645184 13 0.06003602161296778 14 0.040024014408645184 15 0.06003602161296778 16 0.040024014408645184 17 0.06003602161296778 18 0.06003602161296778 19 0.06003602161296778 20 0.06003602161296778 21 0.06003602161296778 22 0.08004802881729037 23 0.040024014408645184 24 0.10006003602161297 25 0.06003602161296778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4997.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.85991594956974 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8997995991984 99.75985591354814 2 0.08016032064128256 0.16009605763458074 3 0.0 0.0 4 0.02004008016032064 0.08004802881729037 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE