FastQCFastQC Report
Thu 26 May 2016
SRR1513147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4997
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCG290.5803482089253552No Hit
TCTCCGAGCCCACGAGACGTAGAGG180.3602161296778067No Hit
TCTCCGAGCCCACGAGACAGGCAGA170.3402041224734841No Hit
TCTCCGAGCCCACGAGACGGACTCC140.2801681008605163No Hit
CTCCGAGCCCACGAGACTAAGGCGA70.14008405043025815No Hit
CTCCGAGCCCACGAGACGGACTCCT70.14008405043025815No Hit
CTCCGAGCCCACGAGACGTAGAGGA70.14008405043025815No Hit
TCTCCGAGCCCACGAGACTAGGCAT70.14008405043025815No Hit
TCTCCGAGCCCACGAGACTCCTGAG60.12007204322593557No Hit
CTCCGAGCCCACGAGACCTCTCTAC50.10006003602161297No Hit
CATCTCCGAGCCCACGAGACTAAGG50.10006003602161297No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)