##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4997 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.378827296377827 7.0 2.0 27.0 2.0 31.0 2 14.582949769861917 7.0 2.0 27.0 2.0 31.0 3 14.852911747048228 10.0 2.0 27.0 2.0 31.0 4 17.034020412247347 16.0 2.0 31.0 2.0 35.0 5 14.62897738643186 2.0 2.0 31.0 2.0 35.0 6 13.467880728437063 2.0 2.0 31.0 2.0 35.0 7 12.63638182909746 2.0 2.0 30.0 2.0 35.0 8 12.282769661797078 2.0 2.0 30.0 2.0 35.0 9 12.425255153091856 2.0 2.0 30.0 2.0 37.0 10 12.175105063037822 2.0 2.0 30.0 2.0 37.0 11 11.904942965779467 2.0 2.0 28.0 2.0 37.0 12 11.630778467080248 2.0 2.0 28.0 2.0 37.0 13 11.590754452671604 2.0 2.0 27.0 2.0 37.0 14 11.6824094456674 2.0 2.0 27.0 2.0 38.0 15 11.520312187312387 2.0 2.0 27.0 2.0 38.0 16 11.368821292775666 2.0 2.0 27.0 2.0 38.0 17 11.13648188913348 2.0 2.0 25.0 2.0 38.0 18 11.113868320992596 2.0 2.0 27.0 2.0 38.0 19 11.065039023414048 2.0 2.0 25.0 2.0 39.0 20 10.955173103862318 2.0 2.0 25.0 2.0 39.0 21 10.792475485291174 2.0 2.0 22.0 2.0 39.0 22 10.739043426055634 2.0 2.0 23.0 2.0 39.0 23 10.620772463478087 2.0 2.0 19.0 2.0 38.0 24 10.450470282169302 2.0 2.0 17.0 2.0 38.0 25 10.39823894336602 2.0 2.0 16.0 2.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 11.501583355880232 1101 2 10.556886949855695 1101 3 12.594373421717172 1101 4 14.85594899891775 1101 5 13.86215559613997 1101 6 14.1641645247114 1101 7 11.654476122835502 1101 8 14.766533639971138 1101 9 9.405706619769118 1101 10 11.116179090007215 1101 11 11.742813740079368 1101 12 12.049997181637806 1101 13 10.919470373376619 1101 14 14.05513956529582 1101 15 16.999084032287158 1101 16 14.058549219877346 1101 17 18.16220068993507 1101 18 16.89643759018759 1101 19 17.11117987914863 1101 20 18.341370174963927 1101 21 16.88499898538961 1101 22 17.80041148088023 1101 23 15.252042748917745 1101 24 17.503610885642132 1101 25 16.769293943903318 1102 1 10.034916689213564 1102 2 9.723553616522363 1102 3 9.594373421717172 1102 4 9.989282332251086 1102 5 4.428822262806637 1102 6 8.430831191378068 1102 7 9.087809456168836 1102 8 8.933200306637806 1102 9 9.772373286435785 1102 10 9.782845756673883 1102 11 10.876147073412701 1102 12 8.416663848304474 1102 13 10.486137040043285 1102 14 11.088472898629151 1102 15 10.532417365620491 1102 16 11.191882553210679 1102 17 9.728867356601736 1102 18 9.963104256854253 1102 19 11.61117987914863 1102 20 9.274703508297259 1102 21 5.918332318722943 1102 22 7.800411480880227 1102 23 8.718709415584414 1102 24 9.603610885642135 1102 25 10.702627277236651 1103 1 9.434916689213566 1103 2 9.323553616522364 1103 3 8.794373421717172 1103 4 9.589282332251084 1103 5 5.62882226280664 1103 6 1.4308311913780667 1103 7 2.4878094561688346 1103 8 2.933200306637806 1103 9 3.3723732864357867 1103 10 3.5828457566738834 1103 11 3.8761470734127013 1103 12 4.016663848304475 1103 13 3.8861370400432875 1103 14 4.088472898629151 1103 15 4.932417365620493 1103 16 4.591882553210677 1103 17 3.9288673566017387 1103 18 4.763104256854254 1103 19 3.211179879148629 1103 20 4.074703508297256 1103 21 4.918332318722943 1103 22 4.800411480880227 1103 23 4.3187094155844115 1103 24 5.203610885642133 1103 25 5.10262727723665 1104 1 -8.307940453643578 1104 2 -7.276446383477637 1104 3 -5.8341980068542565 1104 4 -7.953574810606057 1104 5 -7.685463451479075 1104 6 -4.312025951479075 1104 7 -4.797904829545452 1104 8 -2.2096568362193363 1104 9 -7.570483856421357 1104 10 -6.217154243326117 1104 11 -6.209567212301585 1104 12 -5.783336151695526 1104 13 -5.913862959956713 1104 14 -6.111527101370848 1104 15 -6.381868348665223 1104 16 -5.750974589646465 1104 17 -6.242561214826834 1104 18 -7.15118145743146 1104 19 -5.960248692279942 1104 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-1.5833361516955264 2213 13 0.2861370400432861 2213 14 0.28847289862915204 2213 15 1.232417365620492 2213 16 -0.608117446789322 2213 17 1.128867356601738 2213 18 0.7631042568542536 2213 19 0.711179879148629 2213 20 1.2747035082972573 2213 21 1.9183323187229426 2213 22 3.000411480880226 2213 23 2.918709415584413 2213 24 2.2036108856421333 2213 25 2.50262727723665 2214 1 -5.915083310786436 2214 2 -4.901446383477637 2214 3 -3.6556265782828277 2214 4 -4.685717667748914 2214 5 -9.846177737193361 2214 6 -8.044168808621933 2214 7 -7.387190543831165 2214 8 -6.941799693362194 2214 9 -7.302626713564214 2214 10 -6.842154243326117 2214 11 -6.5488529265872995 2214 12 -6.408336151695526 2214 13 -6.788862959956713 2214 14 -6.736527101370848 2214 15 -6.042582634379508 2214 16 -5.858117446789322 2214 17 -5.921132643398263 2214 18 -6.311895743145746 2214 19 -5.763820120851371 2214 20 -5.575296491702742 2214 21 -5.531667681277057 2214 22 -5.449588519119773 2214 23 -5.331290584415587 2214 24 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-8.9238529265873 2216 12 -5.950002818362193 2216 13 -6.747196293290046 2216 14 -8.028193768037514 2216 15 -6.000915967712841 2216 16 -7.608117446789322 2216 17 -7.421132643398263 2216 18 -5.686895743145746 2216 19 -5.638820120851371 2216 20 -6.991963158369409 2216 21 -5.36500101461039 2216 22 -5.11625518578644 2216 23 -7.581290584415587 2216 24 -7.496389114357866 2216 25 -7.730706056096683 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2300.0 3 0.0 4 40.0 5 279.0 6 279.0 7 171.0 8 101.0 9 83.0 10 67.0 11 51.0 12 47.0 13 43.0 14 40.0 15 30.0 16 29.0 17 28.0 18 27.0 19 29.0 20 32.0 21 46.0 22 39.0 23 39.0 24 35.0 25 36.0 26 37.0 27 29.0 28 29.0 29 43.0 30 38.0 31 58.0 32 56.0 33 66.0 34 107.0 35 123.0 36 126.0 37 185.0 38 229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.381228737242346 15.029017410446269 13.908345007004202 35.68140884530718 2 10.34620772463478 16.36982189313588 37.68260956573945 35.601360816489894 3 11.707024214528717 22.733640184110467 28.457074244546725 37.10226135681409 4 8.445067040224135 11.80708425055033 33.840304182509506 45.90754452671603 5 11.426856113668201 37.282369421652994 35.08104862917751 16.209725835501303 6 33.09985991594957 35.941564938963374 17.290374224534723 13.668200920552332 7 25.99559735841505 32.879727836702024 21.292775665399237 19.831899139483692 8 26.736041624974987 34.60076045627377 18.27096257754653 20.392235341204724 9 23.814288573143884 12.6676005603362 18.811286772063237 44.70682409445668 10 12.567540524314587 27.236341805083047 33.54012407444466 26.655993596157696 11 31.93916349809886 21.572943766259755 23.173904342605564 23.31398839303582 12 21.75305183109866 25.955573344006407 29.097458475085052 23.193916349809886 13 26.61596958174905 19.811887132279367 24.574744846908146 28.99739843906344 14 21.953171903141886 20.67240344206524 25.29517710626376 32.07924754852912 15 22.273364018411044 30.578347008204922 22.0532319391635 25.09505703422053 16 24.014408645187114 26.776065639383628 25.23514108465079 23.974384630778466 17 20.416333066453163 28.282626100880705 25.88070456365092 25.420336269015216 18 19.715772618094476 26.281024819855887 27.401921537229786 26.601281024819855 19 21.933159895937564 27.036221733039824 26.79607764658795 24.234540724434662 20 22.793676205723436 26.736041624974987 27.116269761857115 23.354012407444465 21 23.334000400240143 26.015609365619376 25.715429257554533 24.934960976585952 22 23.05383229937963 26.475885531318795 25.61536922153292 24.85491294776866 23 21.77306383830298 27.296377826696016 25.67540524314589 25.255153091855114 24 22.853712227336402 25.175105063037822 26.09565739443666 25.875525315189112 25 21.813087852711625 26.79607764658795 25.875525315189112 25.51530918551131 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 3.0 9 3.0 10 3.0 11 3.0 12 3.0 13 3.0 14 8.0 15 13.0 16 13.0 17 13.0 18 22.5 19 32.0 20 32.0 21 32.0 22 58.0 23 84.0 24 84.0 25 84.0 26 118.0 27 152.0 28 152.0 29 152.0 30 218.5 31 285.0 32 285.0 33 285.0 34 330.5 35 376.0 36 376.0 37 376.0 38 420.0 39 464.0 40 464.0 41 464.0 42 528.5 43 593.0 44 593.0 45 593.0 46 609.5 47 626.0 48 626.0 49 626.0 50 604.0 51 582.0 52 582.0 53 582.0 54 575.5 55 569.0 56 569.0 57 569.0 58 520.0 59 471.0 60 471.0 61 471.0 62 440.5 63 410.0 64 410.0 65 410.0 66 307.5 67 205.0 68 205.0 69 205.0 70 140.5 71 76.0 72 76.0 73 76.0 74 56.5 75 37.0 76 37.0 77 37.0 78 24.0 79 11.0 80 11.0 81 11.0 82 7.5 83 4.0 84 4.0 85 4.0 86 2.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.020012007204322592 18 0.020012007204322592 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4997.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.21833099859916 #Duplication Level Percentage of deduplicated Percentage of total 1 99.40304652120214 96.6379827896738 2 0.22643062988884316 0.4402641584950971 3 0.08233841086867023 0.24014408645187113 4 0.06175380815150268 0.24014408645187113 5 0.04116920543433512 0.20012007204322593 6 0.02058460271716756 0.12007204322593557 7 0.08233841086867023 0.5603362017210326 8 0.0 0.0 9 0.0 0.0 >10 0.08233841086867023 1.5609365619371625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCCGAGCCCACGAGACTAAGGCG 29 0.5803482089253552 No Hit TCTCCGAGCCCACGAGACGTAGAGG 18 0.3602161296778067 No Hit TCTCCGAGCCCACGAGACAGGCAGA 17 0.3402041224734841 No Hit TCTCCGAGCCCACGAGACGGACTCC 14 0.2801681008605163 No Hit CTCCGAGCCCACGAGACTAAGGCGA 7 0.14008405043025815 No Hit CTCCGAGCCCACGAGACGGACTCCT 7 0.14008405043025815 No Hit CTCCGAGCCCACGAGACGTAGAGGA 7 0.14008405043025815 No Hit TCTCCGAGCCCACGAGACTAGGCAT 7 0.14008405043025815 No Hit TCTCCGAGCCCACGAGACTCCTGAG 6 0.12007204322593557 No Hit CTCCGAGCCCACGAGACCTCTCTAC 5 0.10006003602161297 No Hit CATCTCCGAGCCCACGAGACTAAGG 5 0.10006003602161297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE