FastQCFastQC Report
Thu 26 May 2016
SRR1513146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078190
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31750.15277717629283175No Hit
GTCCTACAGTGGACATTTCTAAATT30210.14536688175768336No Hit
GTCCTAAAGTGTGTATTTCTCATTT27960.13454015272905748No Hit
CCCATGTACTCTGCGTTGATACCAC26780.12886213483848927No Hit
CTTTAGGACGTGAAATATGGCGAGG24550.11813164340122896No Hit
CTGTAGGACGTGGAATATGGCAAGA23950.11524451566026206No Hit
GTATCAACGCAGAGTACTTTTTTTT20970.10090511454679313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7000.012.6547494
TAGACGG550.003043507212.1044045
GGCGAGG7000.011.93143219
TCCAACG1302.6557245E-911.68112218
AAAGGCG1001.9046838E-611.41272455
TGGCGAG15950.011.24628418
TGGACCG855.2644602E-511.1889455
AAGGCGT951.3803501E-510.9857746
GTCCTAC15050.010.9748161
GCGTTTA700.001504329110.84623413
TAGGACT4000.010.7157144
CTAGACT2250.010.5834224
GTCCTAA9450.010.5072541
TCCTACA17150.010.33742
CTAGGAC2800.010.2054423
AGGACCT12250.010.1705235
GTATTAC1605.689799E-910.1441721
TATGGCG18150.010.09226416
TTAGGAC11900.010.0053363
GACGTGA9750.09.9263257
Produced by FastQC (version 0.11.5)