##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513146_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2078190 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.372821060634493 31.0 30.0 34.0 25.0 34.0 2 29.249267872523685 31.0 30.0 34.0 22.0 34.0 3 29.43658472035762 31.0 30.0 34.0 23.0 34.0 4 32.524161890876194 35.0 33.0 37.0 22.0 37.0 5 32.669628859728896 37.0 33.0 37.0 25.0 37.0 6 33.04701013863025 37.0 35.0 37.0 26.0 37.0 7 32.98981373214191 37.0 35.0 37.0 26.0 37.0 8 32.88109508755215 37.0 35.0 37.0 26.0 37.0 9 34.12115975921355 39.0 35.0 39.0 25.0 39.0 10 33.71344872220538 39.0 34.0 39.0 18.0 39.0 11 34.15073742054384 39.0 35.0 39.0 25.0 39.0 12 34.1514534282236 39.0 35.0 39.0 25.0 39.0 13 34.186402109528004 39.0 35.0 39.0 25.0 39.0 14 34.41674967158922 39.0 34.0 41.0 17.0 41.0 15 34.848178944177384 40.0 34.0 41.0 21.0 41.0 16 34.68583863843056 39.0 34.0 41.0 18.0 41.0 17 34.303193163281506 39.0 34.0 41.0 17.0 41.0 18 34.04648371900548 39.0 33.0 41.0 16.0 41.0 19 34.670589310890726 39.0 34.0 41.0 18.0 41.0 20 34.8953945500652 40.0 35.0 41.0 22.0 41.0 21 34.93034082542982 40.0 35.0 41.0 22.0 41.0 22 34.625185377660365 40.0 35.0 41.0 18.0 41.0 23 34.15942863742006 39.0 34.0 41.0 10.0 41.0 24 34.409087234564694 39.0 34.0 41.0 16.0 41.0 25 34.040071408292796 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8456316472504106 1101 2 1.1099274230034482 1101 3 0.9350539728079568 1101 4 1.4298763332268436 1101 5 1.2081694149252158 1101 6 0.9409221184559016 1101 7 0.989752799623723 1101 8 0.9226598115174198 1101 9 1.233768993555472 1101 10 1.4796810133340443 1101 11 1.1947496357108633 1101 12 1.0995355312893764 1101 13 0.9402970447709365 1101 14 1.8977360583644227 1101 15 1.6000446497257457 1101 16 1.6225975517081608 1101 17 2.0824984294591573 1101 18 1.9890208648827752 1101 19 1.6633525580421846 1101 20 1.3103073465817943 1101 21 1.3083464699957545 1101 22 1.5815339311840049 1101 23 1.794683420030978 1101 24 1.4299725398057959 1101 25 1.585558872520707 1102 1 0.8418623094032185 1102 2 0.9991832827574854 1102 3 0.9178448760833824 1102 4 1.2294653178715649 1102 5 1.1962768036180478 1102 6 0.937748159890468 1102 7 0.9094089411347142 1102 8 0.917390046003419 1102 9 1.1828646639381688 1102 10 1.571745577844652 1102 11 1.1631016929439397 1102 12 1.1957306144388298 1102 13 1.1260272289431938 1102 14 2.071645508003961 1102 15 1.6957575595132042 1102 16 1.6547606113586966 1102 17 2.1120805139335346 1102 18 2.170428859017626 1102 19 1.5478064502893147 1102 20 1.3536023216764903 1102 21 1.172541893361938 1102 22 1.4480282133865998 1102 23 1.6944147206891316 1102 24 1.550905212831161 1102 25 1.7948736233119504 1103 1 0.6482930257551516 1103 2 0.8427251085091427 1103 3 0.6182644161532522 1103 4 1.0913433116067068 1103 5 0.9754463014791739 1103 6 0.6190342557685824 1103 7 0.6136881866576758 1103 8 0.6357170514813717 1103 9 0.9198311479273826 1103 10 1.203791401430479 1103 11 0.7408032942300125 1103 12 0.6592052932426569 1103 13 0.7112634112825802 1103 14 1.572230308868555 1103 15 1.1961090521821163 1103 16 1.1855871108254377 1103 17 1.5776980144770647 1103 18 1.733799827737343 1103 19 1.1286713868616758 1103 20 0.8659436637110431 1103 21 0.9085148181581033 1103 22 1.1831365254575843 1103 23 1.5469406398177767 1103 24 1.1932200527134142 1103 25 1.4659651482510512 1104 1 0.6491291331574516 1104 2 0.8700835478796201 1104 3 0.7032206918721577 1104 4 1.103335796320195 1104 5 1.0403481241268224 1104 6 0.7920291893547073 1104 7 0.6693062935505552 1104 8 0.7263764772943162 1104 9 1.02912439712523 1104 10 1.2015814026576024 1104 11 0.8089275184155227 1104 12 0.7454852019758249 1104 13 0.8040147189531908 1104 14 1.6633009927488658 1104 15 1.3805932024663363 1104 16 1.4291184741420224 1104 17 1.9204561911156333 1104 18 1.9738650267100795 1104 19 1.2668986846781252 1104 20 1.0355022555188356 1104 21 1.113960223062989 1104 22 1.279131257130274 1104 23 1.5413398642355176 1104 24 1.2033678406368438 1104 25 1.4889370304607183 1105 1 0.5259790364218766 1105 2 0.7418313934031993 1105 3 0.640979372951108 1105 4 1.0274652493031908 1105 5 0.8968050165488606 1105 6 0.6413057945652554 1105 7 0.5979370973192886 1105 8 0.58952622279633 1105 9 0.8850101447502539 1105 10 1.2971180046094872 1105 11 0.7716566617275973 1105 12 0.6211388622685803 1105 13 0.5891977360299734 1105 14 1.3617677606878758 1105 15 1.0211984364317885 1105 16 1.253992054451082 1105 17 1.6138987580866058 1105 18 1.2271408162782507 1105 19 1.1272671481971557 1105 20 0.8298953970981557 1105 21 0.9079601781831457 1105 22 1.0046911087926347 1105 23 1.2942904700646238 1105 24 1.123414496161601 1105 25 1.3453624094270964 1106 1 0.21480594238253659 1106 2 0.43653651326594556 1106 3 0.26880851869709943 1106 4 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1.0704623345385045 1107 17 1.5785312890272962 1107 18 1.594763338657316 1107 19 1.142555762621761 1107 20 0.9357370324375864 1107 21 0.7974096496009366 1107 22 1.1307391000417795 1107 23 1.391542211812812 1107 24 1.0013588525282415 1107 25 1.3730578786894796 1108 1 0.5535108448336885 1108 2 0.7201912754896966 1108 3 0.615423541636634 1108 4 1.1968558575109896 1108 5 1.0511676491408295 1108 6 0.7335262812772214 1108 7 0.7008909307224442 1108 8 0.6955550211350996 1108 9 0.8590412442845476 1108 10 1.1624919944210816 1108 11 0.7711540341751828 1108 12 0.5702954953584367 1108 13 0.4793184956673642 1108 14 1.54526172991622 1108 15 1.0375950238013232 1108 16 1.0864927982411956 1108 17 1.4604497350321495 1108 18 1.6347607562285802 1108 19 1.145248653628336 1108 20 0.8106538125519904 1108 21 0.8676755949788344 1108 22 1.0532740747847313 1108 23 1.3777571922878593 1108 24 1.1199274107866373 1108 25 1.3093166708890536 1109 1 0.1037571984643968 1109 2 0.27282743643700513 1109 3 0.30266712134223894 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2216 24 -1.645261504014755 2216 25 -1.108479090727215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97702.0 3 5.0 4 793.0 5 4853.0 6 5819.0 7 5121.0 8 4736.0 9 4442.0 10 4211.0 11 4110.0 12 3865.0 13 3839.0 14 3886.0 15 3964.0 16 4110.0 17 4297.0 18 4821.0 19 6031.0 20 7676.0 21 9722.0 22 12161.0 23 14260.0 24 16517.0 25 18800.0 26 21335.0 27 24042.0 28 27036.0 29 32000.0 30 38868.0 31 46693.0 32 59335.0 33 77759.0 34 102919.0 35 139089.0 36 200247.0 37 296059.0 38 767067.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88422513894044 19.045082737644787 12.494517094614462 25.576175028800318 2 14.1116851666932 20.586983892675725 41.862448564137445 23.43888237649363 3 18.157693010905973 25.344432753903828 30.011177571914914 26.486696663275282 4 11.271959790836586 17.229164592974993 39.41412210605254 32.08475351013588 5 12.280174509697984 38.43583816367327 36.565174857019365 12.718812469609379 6 31.179713499868853 37.65563675652783 18.615289471974435 12.549360271628881 7 27.465668576026765 31.77048792155677 22.956646199840797 17.807197302575663 8 24.59706178754893 37.066994824487864 21.18966514619354 17.146278241769668 9 26.460791528080513 14.925915462725758 20.572000872064063 38.041292137129666 10 13.796958682904478 29.06331938257297 36.025159496848566 21.114562437673985 11 33.820739415979 22.436690310241303 25.217465379280608 18.52510489449909 12 22.882221883165734 24.986848191307438 32.31816932598725 19.812760599539576 13 28.690043471731823 22.10276694300159 25.939464939605482 23.267724645661104 14 20.203040163795166 22.177900070125045 28.99182131156457 28.62723845451522 15 22.888248226236822 30.883625057344332 23.603578190234444 22.624548526184398 16 21.656340029203992 28.158512040150196 28.4556535332116 21.729494397434205 17 21.31594984822184 28.59773926120188 28.59716160883885 21.489149281737426 18 21.122421180613383 27.61920255987925 30.341240220677918 20.917136038829458 19 23.122724925265164 27.26783107496394 27.838371567503934 21.771072432266966 20 23.403323405344942 27.55156361003991 28.10127703323213 20.94383595138302 21 22.829659843382302 27.05994532965142 27.282804736171524 22.827590090794754 22 22.90208847621725 28.139887084413257 27.34525134501257 21.612773094356914 23 21.90500393715189 27.84933318701375 28.12300856162076 22.122654314213598 24 22.760405258052323 27.42602403722327 28.20746246741399 21.606108237310412 25 22.532683828484508 27.378939869457223 28.013891830486937 22.074484471571328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 219.0 2 348.0 3 477.0 4 477.0 5 477.0 6 996.0 7 1515.0 8 1515.0 9 1515.0 10 2485.5 11 3456.0 12 3456.0 13 3456.0 14 6514.5 15 9573.0 16 9573.0 17 9573.0 18 17690.0 19 25807.0 20 25807.0 21 25807.0 22 41126.0 23 56445.0 24 56445.0 25 56445.0 26 80605.0 27 104765.0 28 104765.0 29 104765.0 30 134276.5 31 163788.0 32 163788.0 33 163788.0 34 187123.5 35 210459.0 36 210459.0 37 210459.0 38 226905.0 39 243351.0 40 243351.0 41 243351.0 42 253337.5 43 263324.0 44 263324.0 45 263324.0 46 269371.5 47 275419.0 48 275419.0 49 275419.0 50 260957.5 51 246496.0 52 246496.0 53 246496.0 54 216253.5 55 186011.0 56 186011.0 57 186011.0 58 158406.5 59 130802.0 60 130802.0 61 130802.0 62 105797.0 63 80792.0 64 80792.0 65 80792.0 66 61997.5 67 43203.0 68 43203.0 69 43203.0 70 31481.0 71 19759.0 72 19759.0 73 19759.0 74 13920.0 75 8081.0 76 8081.0 77 8081.0 78 5545.0 79 3009.0 80 3009.0 81 3009.0 82 2009.0 83 1009.0 84 1009.0 85 1009.0 86 658.0 87 307.0 88 307.0 89 307.0 90 196.5 91 86.0 92 86.0 93 86.0 94 53.5 95 21.0 96 21.0 97 21.0 98 18.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17130291263070266 2 0.2511801134641202 3 0.25450031036623216 4 0.4143990684201156 5 0.24935159922817451 6 0.017082172467387486 7 0.017130291263070266 8 0.01578296498395238 9 0.017370885241484176 10 0.019536231047209352 11 0.01867009272491928 12 0.025454842916191493 13 0.024925536163680897 14 0.022760190357955724 15 0.039794244029660426 16 0.01886256790765041 17 0.039264937277149824 18 0.02848632704420674 19 0.024588704593901424 20 0.02704276317372329 21 0.03113286080675973 22 0.042633252974944545 23 0.02627286244279878 24 0.04787820170436774 25 0.03416434493477497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2078190.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.97238400325737 #Duplication Level Percentage of deduplicated Percentage of total 1 84.2148845192945 62.295757764512274 2 11.035041781127376 16.32576696251087 3 2.5896021716102813 5.746771387620748 4 0.9153622730633175 2.7084611826053706 5 0.4073016519418328 1.5064537101301168 6 0.21630937303382525 0.9600552003337182 7 0.14085715572900243 0.7293677729234673 8 0.08944843054887125 0.5293370922439842 9 0.06374315716113776 0.4243709969193301 >10 0.29603458516993997 3.7994354934909733 >50 0.016484376832797104 0.8315176167229041 >100 0.012038257207581848 1.7962311626451832 >500 0.0018405337233570137 0.8786451496809957 >1k 0.0010517335562038885 1.4678285076600863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3175 0.15277717629283175 No Hit GTCCTACAGTGGACATTTCTAAATT 3021 0.14536688175768336 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2796 0.13454015272905748 No Hit CCCATGTACTCTGCGTTGATACCAC 2678 0.12886213483848927 No Hit CTTTAGGACGTGAAATATGGCGAGG 2455 0.11813164340122896 No Hit CTGTAGGACGTGGAATATGGCAAGA 2395 0.11524451566026206 No Hit GTATCAACGCAGAGTACTTTTTTTT 2097 0.10090511454679313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 2 0.0 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 3 0.0 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 4 0.0 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 5 4.811879568278165E-5 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 6 4.811879568278165E-5 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 7 1.4435638704834495E-4 0.0 0.0 4.811879568278165E-5 4.811879568278165E-5 8 1.4435638704834495E-4 0.0 0.0 9.62375913655633E-5 4.811879568278165E-5 9 1.4435638704834495E-4 0.0 0.0 1.4435638704834495E-4 1.4435638704834495E-4 10 1.4435638704834495E-4 0.0 0.0 1.4435638704834495E-4 1.4435638704834495E-4 11 1.4435638704834495E-4 0.0 0.0 1.4435638704834495E-4 1.4435638704834495E-4 12 1.924751827311266E-4 0.0 0.0 1.4435638704834495E-4 1.4435638704834495E-4 13 1.924751827311266E-4 0.0 0.0 1.4435638704834495E-4 1.4435638704834495E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 700 0.0 12.654749 4 TAGACGG 55 0.0030435072 12.104404 5 GGCGAGG 700 0.0 11.931432 19 TCCAACG 130 2.6557245E-9 11.681122 18 AAAGGCG 100 1.9046838E-6 11.4127245 5 TGGCGAG 1595 0.0 11.246284 18 TGGACCG 85 5.2644602E-5 11.188945 5 AAGGCGT 95 1.3803501E-5 10.985774 6 GTCCTAC 1505 0.0 10.974816 1 GCGTTTA 70 0.0015043291 10.846234 13 TAGGACT 400 0.0 10.715714 4 CTAGACT 225 0.0 10.583422 4 GTCCTAA 945 0.0 10.507254 1 TCCTACA 1715 0.0 10.3374 2 CTAGGAC 280 0.0 10.205442 3 AGGACCT 1225 0.0 10.170523 5 GTATTAC 160 5.689799E-9 10.144172 1 TATGGCG 1815 0.0 10.092264 16 TTAGGAC 1190 0.0 10.005336 3 GACGTGA 975 0.0 9.926325 7 >>END_MODULE