FastQCFastQC Report
Thu 26 May 2016
SRR1513146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078190
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35630.17144726901775104No Hit
CCCATGTACTCTGCGTTGATACCAC34550.1662504390840106No Hit
GTATCAACGCAGAGTACTTTTTTTT31540.15176668158349332No Hit
GTCCTACAGTGGACATTTCTAAATT30050.14459698102675886No Hit
CTTTAGGACGTGAAATATGGCGAGG29180.14041064580235685No Hit
GTCCTAAAGTGTGTATTTCTCATTT28300.13617619178227208No Hit
CTGTAGGACGTGGAATATGGCAAGA27550.13256728210606347No Hit
TATCAACGCAGAGTACTTTTTTTTT23260.11192431875815011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG500.001473562313.3312351
GGCGAGG8250.012.07702219
TAGGACC6050.011.6426264
TCCAACG1451.2332748E-911.12915518
TGGCGAG18550.011.10445118
GTCCTAA10250.010.962271
TTAGGAC13100.010.5361463
AAGGCGT1553.5524863E-910.4390356
GACGTGA10600.010.2079487
ATGGCGA20550.010.16230317
TATGGCG20250.010.12510516
CCAACGA1502.4894689E-810.12169419
TTTAGGA14900.010.0945582
GGCGTGC856.6605565E-410.0479958
ATTATAC1909.640644E-1110.01985553
AGGACGT19600.010.0042775
GATATAC4500.09.9455241
AGGACCT11700.09.9254125
TAGAAAT5950.09.9185764
CGTGAAA12100.09.8807249
Produced by FastQC (version 0.11.5)