FastQCFastQC Report
Thu 26 May 2016
SRR1513145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509439
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29360.1169982613643926No Hit
GTCCTAAAGTGTGTATTTCTCATTT26870.10707572489309362No Hit
GTCCTACAGTGGACATTTCTAAATT26080.10392761091223976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC500.001515896313.28148
TAGGACC6200.013.21906954
GGTCTAC2400.011.5455051
TGGCGAG15800.010.92969518
GTAGGAC15600.010.8135653
TAGGACT4950.010.7814454
TGTAGGA17700.010.776812
GCGTTTA803.8123687E-410.6753213
ATAGGAC2800.010.5517363
GTATTAG2450.010.5298911
GTCCTAC16300.010.4928051
GTTAGAC1105.821079E-610.3970183
GGCGAGG7750.010.16219319
GGACGGT750.002681237210.1183556
GGACCGA750.002681237210.1183556
CTAAGAC2650.010.0700673
TCCTACA18600.010.0502432
TAGGACA6450.010.0471614
ATGGCGA17800.09.96876417
TAAGACC2201.8189894E-129.963214
Produced by FastQC (version 0.11.5)