##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513145_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2509439
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.317889775364137	31.0	30.0	34.0	24.0	34.0
2	29.166078553812227	31.0	30.0	34.0	22.0	34.0
3	29.35302591535399	31.0	30.0	34.0	23.0	34.0
4	32.424360185682936	35.0	33.0	37.0	20.0	37.0
5	32.57728480349592	35.0	33.0	37.0	23.0	37.0
6	32.95960650966212	37.0	35.0	37.0	26.0	37.0
7	32.90772280178956	37.0	35.0	37.0	26.0	37.0
8	32.78659134571512	37.0	35.0	37.0	25.0	37.0
9	34.02072774034356	39.0	34.0	39.0	23.0	39.0
10	33.59085158077164	38.0	34.0	39.0	18.0	39.0
11	34.0487993531622	39.0	34.0	39.0	25.0	39.0
12	34.043107244288464	39.0	35.0	39.0	25.0	39.0
13	34.08325526143493	39.0	35.0	39.0	25.0	39.0
14	34.28793885804755	39.0	33.0	41.0	17.0	41.0
15	34.72132616094673	39.0	34.0	41.0	19.0	41.0
16	34.56055317543084	39.0	34.0	41.0	17.0	41.0
17	34.163403852414824	39.0	33.0	41.0	17.0	41.0
18	33.89118045905878	39.0	33.0	41.0	16.0	41.0
19	34.531256986123196	39.0	34.0	41.0	18.0	41.0
20	34.748636647473795	39.0	34.0	41.0	19.0	41.0
21	34.78746205825286	40.0	35.0	41.0	19.0	41.0
22	34.46978547794945	40.0	34.0	41.0	17.0	41.0
23	34.007878653356386	39.0	34.0	41.0	10.0	41.0
24	34.257073792190205	39.0	34.0	41.0	10.0	41.0
25	33.89324984588189	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9605928935533434
1101	2	1.2146171612839893
1101	3	1.0833034367365535
1101	4	1.4631305561905918
1101	5	1.3865733352580492
1101	6	1.0804545310900622
1101	7	0.9727606877845858
1101	8	1.0289379203743962
1101	9	1.3936668826740046
1101	10	1.7950940184783093
1101	11	1.2755260173530658
1101	12	1.1295315748068475
1101	13	1.017344237756106
1101	14	2.1266051636443706
1101	15	1.6758828591341555
1101	16	1.782236736843366
1101	17	2.0970845228630424
1101	18	2.0851219166872283
1101	19	1.6431777728524253
1101	20	1.283262896818357
1101	21	1.363317638426345
1101	22	1.6458474741574705
1101	23	1.953059130726551
1101	24	1.625498522669794
1101	25	1.8745138611086034
1102	1	0.7788149371878212
1102	2	0.9726549077292859
1102	3	0.8782809855027658
1102	4	1.2443870574689981
1102	5	1.1053195030457843
1102	6	0.8463645601609429
1102	7	0.738945813481152
1102	8	0.8649326539047095
1102	9	1.1292598303431234
1102	10	1.3807564532735697
1102	11	0.9415581287781691
1102	12	0.9249575266854109
1102	13	0.9058601967607132
1102	14	1.886473304464296
1102	15	1.4379630342249001
1102	16	1.5063081669034162
1102	17	1.8699160620111144
1102	18	2.1010271377827934
1102	19	1.4374847644529112
1102	20	1.2448128418100026
1102	21	1.1293162219931645
1102	22	1.3437896972848193
1102	23	1.579087717521837
1102	24	1.413788049802676
1102	25	1.6859320529515713
1103	1	0.9219202011319503
1103	2	1.242011538069434
1103	3	0.9401458570038912
1103	4	1.368182428089554
1103	5	1.2404142091377537
1103	6	0.964003625084132
1103	7	0.9967446588121547
1103	8	1.0008251887885038
1103	9	1.373731047955168
1103	10	1.7492735142063154
1103	11	1.340364547318778
1103	12	1.241493235433957
1103	13	1.2467826208549369
1103	14	2.117723058813958
1103	15	1.7174475651788725
1103	16	1.7295188643357235
1103	17	2.113712511680241
1103	18	2.3906247759427117
1103	19	1.711733661800153
1103	20	1.4551445723791119
1103	21	1.5025099577681047
1103	22	1.7916521441173785
1103	23	2.1381497458459933
1103	24	1.7987068666259134
1103	25	1.9591810899659805
1104	1	0.8827683472156025
1104	2	1.1560072645651616
1104	3	1.0601278442866011
1104	4	1.3593683255254518
1104	5	1.2142041571124835
1104	6	1.0187566082791477
1104	7	0.9239673455457122
1104	8	0.9480889589689383
1104	9	1.3415855733278121
1104	10	1.482295624945813
1104	11	1.0951154660189673
1104	12	0.9510549431840758
1104	13	0.9810408478667867
1104	14	1.970280709831755
1104	15	1.5749871002546385
1104	16	1.6433313669163425
1104	17	2.0377107155697516
1104	18	2.2314625479399055
1104	19	1.5469250404697874
1104	20	1.1829744786510474
1104	21	1.2114781232580114
1104	22	1.56879638004785
1104	23	1.8065428463043105
1104	24	1.5770885091379938
1104	25	1.7782655381265187
1105	1	0.411594336347882
1105	2	0.6076333187905547
1105	3	0.5129260697917815
1105	4	0.9218689623058864
1105	5	0.7781126446881856
1105	6	0.5408802002868427
1105	7	0.5096300924750139
1105	8	0.5819322119264356
1105	9	0.7565083813880378
1105	10	1.0965921948218522
1105	11	0.6641700593285265
1105	12	0.6911084327408759
1105	13	0.5113753698569425
1105	14	1.3047843748569434
1105	15	1.0406818984659907
1105	16	1.1781338234737717
1105	17	1.480570708777428
1105	18	1.2006723741695993
1105	19	0.9279642661447767
1105	20	0.6925215154994078
1105	21	0.7392496747381685
1105	22	0.9676458593029551
1105	23	1.2415599686227594
1105	24	1.0008964561578608
1105	25	1.3476572713013582
1106	1	0.5059161480617433
1106	2	0.8181258706413317
1106	3	0.7048101530602402
1106	4	1.1442323314457283
1106	5	0.9728445148072709
1106	6	0.662147939170481
1106	7	0.6376905713768721
1106	8	0.6256096112359018
1106	9	0.8247651269640031
1106	10	0.8186456986113839
1106	11	0.6876899425879088
1106	12	0.7247816777041649
1106	13	0.4601644303114014
1106	14	1.5846936774504456
1106	15	1.096828829660943
1106	16	1.309782330876537
1106	17	1.4561032854844598
1106	18	1.5447360927632658
1106	19	1.1105072147530706
1106	20	0.9363967175437651
1106	21	0.7449340072673962
1106	22	1.0430242713652262
1106	23	1.4393202523750759
1106	24	0.9880663689165914
1106	25	1.3965141278926296
1107	1	0.5446564054575518
1107	2	0.7817859258185749
1107	3	0.6801679884752225
1107	4	1.059309904856164
1107	5	0.9242953090927699
1107	6	0.7507479241407964
1107	7	0.706219966748229
1107	8	0.6365464790775661
1107	9	1.020484190564801
1107	10	1.2614461057993438
1107	11	0.8310397096694118
1107	12	0.814138138263587
1107	13	0.7375590096979465
1107	14	1.6151244886346348
1107	15	1.0563880273420452
1107	16	1.1061607594537861
1107	17	1.6222773741435077
1107	18	1.8322540791627873
1107	19	1.2611038127758647
1107	20	0.9506971010225413
1107	21	0.8621253309804402
1107	22	1.132872108297036
1107	23	1.473780793493603
1107	24	1.1058233855188462
1107	25	1.3918417541981256
1108	1	0.6710954423799471
1108	2	0.8760389573317546
1108	3	0.7582703756925575
1108	4	1.2521955067335
1108	5	1.0518737349246763
1108	6	0.7054033414862815
1108	7	0.5910947045774293
1108	8	0.7381082471006479
1108	9	0.8996847960662961
1108	10	1.139830902866997
1108	11	0.8950100624739719
1108	12	0.7132516391056001
1108	13	0.6807482992520946
1108	14	1.617729155308723
1108	15	1.1514082555348537
1108	16	1.1872495106667031
1108	17	1.5487565768672908
1108	18	1.863381896302208
1108	19	1.1938016537893574
1108	20	1.0486064697025057
1108	21	1.0579474630159424
1108	22	1.2110714474487523
1108	23	1.5242943807504759
1108	24	1.236678080640317
1108	25	1.6330542291148689
1109	1	0.42575501626871315
1109	2	0.6165950150314039
1109	3	0.6804104722928592
1109	4	0.9262658476000638
1109	5	0.9569699695202303
1109	6	0.6728585315770346
1109	7	0.5898172624620699
1109	8	0.5711585348888306
1109	9	0.9050122542247578
1109	10	1.2087377669127761
1109	11	0.7840307604797658
1109	12	0.7948521749078807
1109	13	0.677538076938994
1109	14	1.6256595469259523
1109	15	1.1300521087839712
1109	16	1.25747746154331
1109	17	1.52302247809196
1109	18	1.7599186948034955
1109	19	1.1220781706901377
1109	20	0.8963044311873105
1109	21	0.9447641696257492
1109	22	1.2274383492185166
1109	23	1.5311443320872868
1109	24	1.2044152930056242
1109	25	1.487886833612599
1110	1	0.5160756807341151
1110	2	0.7155449115254306
1110	3	0.6052090017371832
1110	4	0.9098813361799998
1110	5	0.7820888193436133
1110	6	0.47015486647067206
1110	7	0.5855415895532161
1110	8	0.5702931177268127
1110	9	0.7759284627986958
1110	10	1.0621249768766603
1110	11	0.7518926959681096
1110	12	0.5759507785841649
1110	13	0.4279003678498583
1110	14	1.2654368217034317
1110	15	0.9660336844273516
1110	16	1.178139152917062
1110	17	1.3521534680283693
1110	18	0.8405802021310649
1110	19	0.6644524704811019
1110	20	0.48258948298297355
1110	21	0.43437162845741994
1110	22	0.9069307231715555
1110	23	-0.36756731795609454
1110	24	0.3386631802754678
1110	25	0.8025806007685716
1111	1	0.25456524757262855
1111	2	0.7011113974224585
1111	3	0.5884948517517117
1111	4	0.9120642846296363
1111	5	0.7904674103852258
1111	6	0.5491642140348532
1111	7	0.5379945823735781
1111	8	0.5707611162771755
1111	9	0.8698070652676577
1111	10	1.0435265564615577
1111	11	0.7708059885765834
1111	12	0.6318193054041288
1111	13	0.4784572711059951
1111	14	1.4790253003326725
1111	15	1.0422109453265804
1111	16	1.0136305122154923
1111	17	1.3759718320903644
1111	18	1.6069629266048366
1111	19	1.0798948554649215
1111	20	0.7364514341849642
1111	21	0.5415030069537821
1111	22	0.9582720643494795
1111	23	1.2920721657893282
1111	24	0.9709492686316636
1111	25	1.215019162490897
1112	1	0.06080799680565718
1112	2	0.2878929592054398
1112	3	0.2329289696717396
1112	4	0.6674865692979708
1112	5	0.37590374542867266
1112	6	0.19928061301394706
1112	7	-0.03429472645108689
1112	8	0.13140085950334424
1112	9	0.33872260833398826
1112	10	0.6235921678462262
1112	11	0.12296613707228943
1112	12	0.18762082129781277
1112	13	0.033282479283570865
1112	14	0.6910303790921191
1112	15	0.548162374855572
1112	16	0.41770383197108885
1112	17	0.8809499186612939
1112	18	1.0481479275071592
1112	19	0.4475791216228515
1112	20	0.23723645055558507
1112	21	0.19112893182938961
1112	22	0.4091965532509718
1112	23	0.8546252091779181
1112	24	0.5613043950850383
1112	25	0.8437383870133175
1113	1	-0.13900924636866563
1113	2	0.01797423109671925
1113	3	-0.0722556707700086
1113	4	0.2066259231050509
1113	5	0.25859298102348305
1113	6	-0.1181809302511283
1113	7	-0.15470322033112893
1113	8	-0.12107093872042185
1113	9	-0.03424725790367944
1113	10	0.2679270005652512
1113	11	-0.05706609937885787
1113	12	-0.2040743896595103
1113	13	-0.3557432945217798
1113	14	0.24419102799785009
1113	15	-0.044219396245146925
1113	16	0.1080260889935758
1113	17	0.42031755854666386
1113	18	0.34866063705304384
1113	19	0.08220642156670266
1113	20	-0.12472225935167103
1113	21	-0.13861520861466659
1113	22	0.0571695673796313
1113	23	0.03493192636781117
1113	24	-0.0073862332595950875
1113	25	0.4563467653171287
1114	1	-1.144341828667006
1114	2	-0.8298008026860764
1114	3	-0.7988760734473743
1114	4	-0.5000462193016091
1114	5	-3.702109668504683
1114	6	-1.532553670286866
1114	7	-1.1903992235504184
1114	8	-0.8504973866949221
1114	9	-0.6332045328493834
1114	10	-0.39362628880012807
1114	11	-0.8910909787492898
1114	12	-0.9234657255788719
1114	13	-0.9576878378708713
1114	14	-6.7134072753579765
1114	15	-2.87054382832714
1114	16	-1.6142857390307697
1114	17	-0.6313510095114125
1114	18	-6.489434589706985
1114	19	-2.6651694453036576
1114	20	-1.5644547081475295
1114	21	-1.3846650710905664
1114	22	-1.1294233304786943
1114	23	-0.6447981094608721
1114	24	-0.7114209624472494
1114	25	-0.5509756379180004
1115	1	-0.9909227262037312
1115	2	-0.6173875169817933
1115	3	-0.6257646147036837
1115	4	-3.1832588842213276
1115	5	-1.2315558497888297
1115	6	-0.947981062262869
1115	7	-0.8601858502922681
1115	8	-0.6654757673675249
1115	9	-0.43680436402951983
1115	10	-0.8135018746560334
1115	11	-0.900037026416129
1115	12	-0.8906196865340377
1115	13	-0.9466546450460953
1115	14	-6.1171220745819
1115	15	-2.5990719325000455
1115	16	-1.5087297362317145
1115	17	-0.6404326989867073
1115	18	-0.3193284485032635
1115	19	-0.7378336790715352
1115	20	-0.861708193749017
1115	21	-1.0196361730736783
1115	22	-1.9817076147496238
1115	23	-1.1951924353222765
1115	24	-0.8122449834563668
1115	25	-0.5204600418490628
1116	1	-0.17768242983559546
1116	2	0.0614649954203621
1116	3	-0.019492344901237857
1116	4	0.23634163206965297
1116	5	-0.42452197948435355
1116	6	-0.196586339745501
1116	7	-0.14670476075666983
1116	8	-0.11334630479029784
1116	9	-0.09466769905054662
1116	10	0.12388595922758583
1116	11	-0.16848812971473848
1116	12	-0.4295316722338214
1116	13	-0.46355057628336027
1116	14	0.5142468809272245
1116	15	0.05895660612550557
1116	16	-0.022017335502440005
1116	17	0.4449668679183745
1116	18	0.34083347479947435
1116	19	0.11486294293072774
1116	20	-0.3260741328067098
1116	21	-0.37705357721399224
1116	22	-0.2093653128810402
1116	23	-0.6942582624218474
1116	24	-0.5520754536567267
1116	25	0.03604315644518152
1201	1	1.013283589149406
1201	2	1.1985960673590839
1201	3	1.1815783796028967
1201	4	1.513998837512787
1201	5	1.3559677240131336
1201	6	1.1089198242039373
1201	7	1.017381037075772
1201	8	1.1390546719949697
1201	9	1.30616534830056
1201	10	1.8131880097070052
1201	11	1.2581070405154193
1201	12	1.2837087164349228
1201	13	1.0739921694939838
1201	14	1.9373036031087096
1201	15	1.6484924152303009
1201	16	1.8035574009238573
1201	17	2.034420967814121
1201	18	1.439859237449511
1201	19	1.4090654724721219
1201	20	1.2924969119448093
1201	21	1.1925476449449164
1201	22	1.5273273759722272
1201	23	1.7605388852414592
1201	24	1.5233720184799893
1201	25	1.8210741347785344
1202	1	1.0150379743546338
1202	2	1.112066027526911
1202	3	1.1191544830707052
1202	4	1.5100623143724263
1202	5	1.3268067804427162
1202	6	0.9603888773367899
1202	7	0.9163070435757064
1202	8	1.0133880359284646
1202	9	1.2858791011101474
1202	10	1.6161490227917525
1202	11	1.161948197963639
1202	12	1.1133478741337228
1202	13	1.1775992645744608
1202	14	2.0392074816503154
1202	15	1.6297362482873652
1202	16	1.7291955441984328
1202	17	2.1174391339175713
1202	18	2.122893670714312
1202	19	1.7881502331875225
1202	20	1.6065878677495107
1202	21	1.4670295784363319
1202	22	1.6405968329117329
1202	23	1.979005851789708
1202	24	1.5616300869631559
1202	25	2.0291135189185283
1203	1	0.6873559257518558
1203	2	0.874123385015718
1203	3	0.7065684538977415
1203	4	1.0625312139615133
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2211	15	-0.31778442361444803
2211	16	-6.597040219491827
2211	17	-1.971846038651634
2211	18	-0.8039567245220525
2211	19	-0.8039778675977516
2211	20	-1.0277627008361279
2211	21	-0.9138019997355258
2211	22	-0.7674619584970728
2211	23	-0.28620392250952875
2211	24	-0.7328708404555044
2211	25	-8.054468462290412
2212	1	-1.652886994074624
2212	2	-4.145189622418194
2212	3	-1.9005851941395306
2212	4	-3.775720827413
2212	5	-1.5818376074595975
2212	6	-1.2596446794586953
2212	7	-1.1047549512455674
2212	8	-1.0150509266775352
2212	9	-1.0001026459416948
2212	10	-0.5436556133296762
2212	11	-1.12460641286372
2212	12	-1.1818343555663873
2212	13	-1.4684496148046904
2212	14	-6.3978599429639935
2212	15	-5.212889688061519
2212	16	-3.008413173448581
2212	17	-7.63829652996737
2212	18	-3.2684982898943957
2212	19	-2.2654216637925586
2212	20	-2.335008600074559
2212	21	-1.775493214328442
2212	22	-1.6983553744171758
2212	23	-1.2624250804184456
2212	24	-1.5673064193079043
2212	25	-1.2450395045656109
2213	1	-0.3829110317937463
2213	2	-0.411530485245148
2213	3	-0.4060789817193786
2213	4	0.009292724005426578
2213	5	-0.1423518546388678
2213	6	-0.5483296487832661
2213	7	-0.535735554981656
2213	8	-0.5294502566251182
2213	9	-0.3551216257867651
2213	10	-3.9409682443470366
2213	11	-1.7741997229495539
2213	12	-1.2388001088316543
2213	13	-1.0500463090457544
2213	14	-0.2603205828797712
2213	15	-0.7858136095922958
2213	16	-0.49710560908555834
2213	17	-0.09439206487309093
2213	18	0.07562073344071507
2213	19	-0.48579877765388346
2213	20	-0.9226407272484565
2213	21	-0.8470623010197613
2213	22	-1.1722351005975327
2213	23	-1.3505550743207522
2213	24	-0.8584397373770472
2213	25	-0.5244777898139859
2214	1	-1.01120979343807
2214	2	-0.6431969295858408
2214	3	-0.5769781791226123
2214	4	-0.07250100195557962
2214	5	-3.7188792340826105
2214	6	-1.4548387974971106
2214	7	-1.1691978373801106
2214	8	-0.7953576800894027
2214	9	-0.5507073435661738
2214	10	-3.9807896054266507
2214	11	-1.575323580749867
2214	12	-1.1199724336943646
2214	13	-0.8659437278411914
2214	14	-0.11481379082125898
2214	15	-0.46405512229822676
2214	16	-0.42510256321970985
2214	17	-0.13469976402158323
2214	18	0.1637229589237208
2214	19	-0.638333849576334
2214	20	-0.838187426819367
2214	21	-0.8775876572971342
2214	22	-0.577874398936757
2214	23	-0.34433272611381227
2214	24	-0.8245072355428604
2214	25	-0.4988380468228115
2215	1	-2.3214865894363435
2215	2	-4.826742298640202
2215	3	-3.057813829001276
2215	4	-2.0399767519889167
2215	5	-2.0272613453501975
2215	6	-2.285777663931679
2215	7	-2.432046010310007
2215	8	-2.541254804568613
2215	9	-8.568499596493428
2215	10	-4.141763932079751
2215	11	-3.3190409146729607
2215	12	-3.620174661945363
2215	13	-3.034779227645817
2215	14	-7.892560064408549
2215	15	-4.89563268618307
2215	16	-9.440801387856574
2215	17	-4.715679575578768
2215	18	-3.2260749568591933
2215	19	-3.7558244560101883
2215	20	-3.6246604708737635
2215	21	-3.646785867002137
2215	22	-9.735952889052548
2215	23	-11.004260019191683
2215	24	-7.1326460572804535
2215	25	-5.401589721681603
2216	1	-1.74800221512497
2216	2	-1.7072611120790278
2216	3	-1.6396314292080731
2216	4	-6.778871992151483
2216	5	-3.3311333878250515
2216	6	-2.469817669889224
2216	7	-2.04020540796148
2216	8	-7.730905119257397
2216	9	-3.714067210927883
2216	10	-2.0848072932827506
2216	11	-2.0614560904655654
2216	12	-2.1946484719725206
2216	13	-1.9999716645852033
2216	14	-1.325143927069135
2216	15	-1.9619000650291127
2216	16	-1.6476764718401995
2216	17	-1.2930217350397513
2216	18	-7.6460765546721845
2216	19	-4.715775990814219
2216	20	-3.9573303144299636
2216	21	-3.060057823889885
2216	22	-2.7037371059723334
2216	23	-1.8238588947672056
2216	24	-2.30172914188379
2216	25	-1.7073966175123445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119071.0
3	4.0
4	973.0
5	5838.0
6	7091.0
7	6217.0
8	5747.0
9	5537.0
10	5232.0
11	4939.0
12	4844.0
13	4878.0
14	4915.0
15	4977.0
16	5000.0
17	5388.0
18	6099.0
19	7421.0
20	9565.0
21	12127.0
22	15293.0
23	18182.0
24	20921.0
25	24044.0
26	27367.0
27	30374.0
28	34011.0
29	41030.0
30	48729.0
31	58483.0
32	74987.0
33	97208.0
34	127734.0
35	169824.0
36	240559.0
37	349492.0
38	905338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.586346101409774	18.389998351209865	12.134536484372225	25.88911906300814
2	14.50886275612818	20.34059064798042	41.082748089651155	24.067798506240244
3	18.358454169590015	25.007032022873254	29.583400590370285	27.051113217166446
4	11.40157639207528	16.875787255135126	38.920852882084496	32.8017834707051
5	12.763169249749895	37.9903809581646	36.16146054738565	13.084989244699857
6	31.785861188257087	37.11190967383992	18.33626151476792	12.765967623135078
7	27.975000229176523	31.35736670993785	22.7334342241458	17.934198836739824
8	25.23829907310867	36.29734495215685	20.895548562154648	17.568807412579833
9	26.70826688816606	14.74215820811882	20.133513434445575	38.41606146926955
10	13.953258493247054	28.664138339235645	35.689952389588285	21.692650777929018
11	34.45688271147313	22.171297337025752	24.569456866768384	18.802363084732736
12	23.12636897228672	24.65471504449471	31.81392938644132	20.404986596777245
13	28.941595990671047	21.67422886865127	25.656054073165553	23.728121067512134
14	20.602549579361657	21.419073455659785	28.673400812976375	29.304976152002187
15	23.433991809970912	30.0370034316477	23.31590932285554	23.213095435525847
16	22.45180869597797	27.64857366175789	27.489301117976872	22.410316524287268
17	21.924250669249982	27.912837041865252	28.01038907194014	22.152523216944633
18	21.77114990289652	27.135279973723065	29.44156730435005	21.65200281903037
19	23.569557990569084	26.895650059590952	27.309637790630468	22.22515415920949
20	23.842563250142504	27.027332990524933	27.465370372584896	21.664733386747663
21	23.525090091526614	26.547102720285743	26.75642599738495	23.17138119080269
22	23.480797452019043	27.37775874792044	26.86172004661252	22.279723753448
23	22.577430541714833	27.187148722445887	27.5289990831905	22.706421652648782
24	23.225061556715104	26.858622086421803	27.466773726445787	22.44954263041731
25	23.01779219267487	26.868076583924523	27.36669807966144	22.74743314373917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	200.0
1	200.0
2	334.5
3	469.0
4	469.0
5	469.0
6	970.0
7	1471.0
8	1471.0
9	1471.0
10	2397.0
11	3323.0
12	3323.0
13	3323.0
14	6272.0
15	9221.0
16	9221.0
17	9221.0
18	17722.5
19	26224.0
20	26224.0
21	26224.0
22	42549.0
23	58874.0
24	58874.0
25	58874.0
26	84653.0
27	110432.0
28	110432.0
29	110432.0
30	143095.5
31	175759.0
32	175759.0
33	175759.0
34	205583.5
35	235408.0
36	235408.0
37	235408.0
38	258368.5
39	281329.0
40	281329.0
41	281329.0
42	298659.5
43	315990.0
44	315990.0
45	315990.0
46	327173.5
47	338357.0
48	338357.0
49	338357.0
50	325387.0
51	312417.0
52	312417.0
53	312417.0
54	279500.5
55	246584.0
56	246584.0
57	246584.0
58	211076.0
59	175568.0
60	175568.0
61	175568.0
62	142933.5
63	110299.0
64	110299.0
65	110299.0
66	85380.0
67	60461.0
68	60461.0
69	60461.0
70	44507.0
71	28553.0
72	28553.0
73	28553.0
74	20138.5
75	11724.0
76	11724.0
77	11724.0
78	8089.5
79	4455.0
80	4455.0
81	4455.0
82	3036.0
83	1617.0
84	1617.0
85	1617.0
86	1064.5
87	512.0
88	512.0
89	512.0
90	325.0
91	138.0
92	138.0
93	138.0
94	89.0
95	40.0
96	40.0
97	40.0
98	27.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1821921154489111
2	0.25874308959094044
3	0.26312653943769904
4	0.4716990530552845
5	0.25850399232657184
6	0.01940672795792207
7	0.018171392092017378
8	0.01621876443300674
9	0.019048082061369095
10	0.021279656528809823
11	0.020522515191642436
12	0.028452574459869318
13	0.02749618540239472
14	0.025224761390892546
15	0.040766083574854776
16	0.02119995744068694
17	0.04120442855953064
18	0.03088339664761726
19	0.026659344977104444
20	0.029847308502019776
21	0.035027749230007184
22	0.04383449846758578
23	0.029448813061405357
24	0.04714201062468544
25	0.03678112916871062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2509439.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.16477717370287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.10597132071175	62.37700621978431
2	10.844643599812624	16.08581152216653
3	2.6914777361970144	5.988385397191015
4	0.9587414276690159	2.844193774010814
5	0.46446309460636626	1.7223400958444817
6	0.24722000842877195	1.1001010102800493
7	0.16021875368012517	0.831781171601441
8	0.10619341162377696	0.6300648568314183
9	0.06968035748268137	0.46510453674783214
>10	0.32421639752818115	4.048661921568023
>50	0.015315510745094617	0.7826872596457207
>100	0.00974148932163245	1.410441328477305
>500	0.0013569821749865282	0.6757391003006175
>1k	7.59910017965318E-4	1.0376818055504116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2936	0.1169982613643926	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2687	0.10707572489309362	No Hit
GTCCTACAGTGGACATTTCTAAATT	2608	0.10392761091223976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.984954406144162E-5	0.0	0.0	0.0
3	0.0	3.984954406144162E-5	0.0	0.0	0.0
4	0.0	3.984954406144162E-5	0.0	0.0	0.0
5	0.0	3.984954406144162E-5	0.0	0.0	0.0
6	0.0	3.984954406144162E-5	0.0	0.0	0.0
7	0.0	3.984954406144162E-5	0.0	0.0	0.0
8	0.0	3.984954406144162E-5	0.0	0.0	0.0
9	0.0	3.984954406144162E-5	0.0	0.0	0.0
10	0.0	3.984954406144162E-5	0.0	0.0	0.0
11	0.0	3.984954406144162E-5	0.0	3.984954406144162E-5	0.0
12	0.0	3.984954406144162E-5	0.0	3.984954406144162E-5	0.0
13	0.0	3.984954406144162E-5	0.0	3.984954406144162E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATC	50	0.0015158963	13.2814	8
TAGGACC	620	0.0	13.2190695	4
GGTCTAC	240	0.0	11.545505	1
TGGCGAG	1580	0.0	10.929695	18
GTAGGAC	1560	0.0	10.813565	3
TAGGACT	495	0.0	10.781445	4
TGTAGGA	1770	0.0	10.77681	2
GCGTTTA	80	3.8123687E-4	10.67532	13
ATAGGAC	280	0.0	10.551736	3
GTATTAG	245	0.0	10.529891	1
GTCCTAC	1630	0.0	10.492805	1
GTTAGAC	110	5.821079E-6	10.397018	3
GGCGAGG	775	0.0	10.162193	19
GGACGGT	75	0.0026812372	10.118355	6
GGACCGA	75	0.0026812372	10.118355	6
CTAAGAC	265	0.0	10.070067	3
TCCTACA	1860	0.0	10.050243	2
TAGGACA	645	0.0	10.047161	4
ATGGCGA	1780	0.0	9.968764	17
TAAGACC	220	1.8189894E-12	9.96321	4
>>END_MODULE