FastQCFastQC Report
Thu 26 May 2016
SRR1513145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509439
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32530.1296305668318696No Hit
GTACATGGGAAGCAGTGGTATCAAC31310.12476892245637372No Hit
CCCATGTACTCTGCGTTGATACCAC31060.12377268385483768No Hit
GTCCTAAAGTGTGTATTTCTCATTT28020.11165842246015942No Hit
GTCCTACAGTGGACATTTCTAAATT27380.10910805164022716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6450.012.1048244
ACCGTGC752.0971675E-411.3868358
GCGCCAC1553.1832315E-1011.02127513
GGCGAGG7800.010.70386519
GTCCTAC16600.010.67355251
GGACGTG14200.010.6605216
GTCCTAA8450.010.5968121
TGGCGAG16850.010.53169118
AGGACGT15000.010.4101485
TCCTACA18300.010.2513312
GTAGGAC15300.010.2068443
AATACCG750.00259938210.1562425
GGACCGT750.002601107610.155436
ACCCGTC750.002668748410.1240527
TATGGCG17950.010.04569916
GACGTGA8800.09.9227227
CCTACAG18750.09.9031283
GTGCGCC1252.6089365E-69.86878711
TGTAGGA17200.09.7996362
ATGGCGA18950.09.76599317
Produced by FastQC (version 0.11.5)