##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2509439 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.7564742557998 31.0 31.0 34.0 27.0 34.0 2 30.84312948033405 33.0 31.0 34.0 27.0 34.0 3 30.952289336381558 33.0 31.0 34.0 27.0 34.0 4 34.34688470211868 37.0 35.0 37.0 31.0 37.0 5 34.09421906649255 37.0 35.0 37.0 30.0 37.0 6 34.01444824919036 37.0 35.0 37.0 30.0 37.0 7 33.878354883302606 37.0 35.0 37.0 29.0 37.0 8 33.84475215376823 37.0 35.0 37.0 29.0 37.0 9 35.39749402157215 39.0 35.0 39.0 30.0 39.0 10 35.24070280249889 39.0 35.0 39.0 29.0 39.0 11 35.261397467720876 39.0 35.0 39.0 29.0 39.0 12 35.1981634142133 39.0 35.0 39.0 29.0 39.0 13 35.171440309965696 39.0 35.0 39.0 29.0 39.0 14 36.32282314891894 40.0 36.0 41.0 28.0 41.0 15 36.307876381932374 40.0 36.0 41.0 28.0 41.0 16 36.26066503310103 40.0 36.0 41.0 27.0 41.0 17 36.195269938818996 40.0 36.0 41.0 27.0 41.0 18 36.15020727740344 40.0 36.0 41.0 27.0 41.0 19 36.23063361970544 40.0 37.0 41.0 27.0 41.0 20 36.1917966525586 40.0 37.0 41.0 27.0 41.0 21 36.13566338930733 40.0 37.0 41.0 27.0 41.0 22 36.07928385587376 40.0 37.0 41.0 27.0 41.0 23 35.998416379119 40.0 37.0 41.0 27.0 41.0 24 35.93960482801136 40.0 36.0 41.0 27.0 41.0 25 35.88321055024649 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5160343918917114 1101 2 0.557140041188898 1101 3 0.5227222249734886 1101 4 0.626961106810576 1101 5 0.6126063968636544 1101 6 0.4908698262576934 1101 7 0.5909353962637454 1101 8 0.6062978347173242 1101 9 0.6737960593527745 1101 10 0.7797940298241386 1101 11 0.7161806308904346 1101 12 0.702599773467881 1101 13 0.7004061698053832 1101 14 0.8359602543526705 1101 15 0.8496966361463905 1101 16 0.8520548421994576 1101 17 0.9047990833220751 1101 18 0.77746925587833 1101 19 0.8275151905317699 1101 20 0.7911980974241501 1101 21 0.813716040624854 1101 22 0.8238680284125621 1101 23 0.8547780949393484 1101 24 0.8712457335436596 1101 25 0.8259414291371456 1102 1 0.35128983066242014 1102 2 0.321923943721913 1102 3 0.18445041239604265 1102 4 0.4054300792822687 1102 5 0.3982427259982728 1102 6 0.32520798377573357 1102 7 0.304010712557222 1102 8 0.34345931577847466 1102 9 0.27673590774250556 1102 10 0.3017549963770847 1102 11 0.3662826586860177 1102 12 0.34742684905985044 1102 13 0.40473279915143934 1102 14 0.38092696232283174 1102 15 0.309013011525316 1102 16 0.2744665153053987 1102 17 0.4393290114145074 1102 18 0.5241023386241324 1102 19 0.40182509982373915 1102 20 0.3774993530918067 1102 21 0.41846266398196263 1102 22 0.3668175805030458 1102 23 0.45374096164800903 1102 24 0.51162424368556 1102 25 0.4740429735758198 1103 1 0.4162263647670983 1103 2 0.4763305010885013 1103 3 0.4987795103186876 1103 4 0.5272797541826222 1103 5 0.44177269127872165 1103 6 0.4688377610493788 1103 7 0.4580781818097819 1103 8 0.4803483752266544 1103 9 0.5933653805910168 1103 10 0.6299954814964366 1103 11 0.5508941642154852 1103 12 0.5452216740647131 1103 13 0.6836812866197519 1103 14 0.7091296662387165 1103 15 0.6136448855469752 1103 16 0.6224839738472383 1103 17 0.7398390359063001 1103 18 0.6401888934033266 1103 19 0.5979191180222401 1103 20 0.6845121680989337 1103 21 0.6048765722219329 1103 22 0.6277750359672751 1103 23 0.6059683195033045 1103 24 0.6834781027998176 1103 25 0.7243283630695672 1104 1 0.41025352319643105 1104 2 0.4931174245783225 1104 3 0.4302963462739271 1104 4 0.5016748572009178 1104 5 0.5974872129352704 1104 6 0.4741938347931338 1104 7 0.5386515436331436 1104 8 0.5618043085847617 1104 9 0.5754404043327099 1104 10 0.6591164712152562 1104 11 0.597384006900981 1104 12 0.6689791062460628 1104 13 0.5641405851044183 1104 14 0.6848370137757769 1104 15 0.6107171738330308 1104 16 0.6067705876628366 1104 17 0.6799712667993987 1104 18 0.7805035740259783 1104 19 0.746876290077239 1104 20 0.7843046863697154 1104 21 0.7457047959305925 1104 22 0.642705204430051 1104 23 0.5337897097341155 1104 24 0.528697765048463 1104 25 0.6120688767158811 1105 1 0.24876862196814997 1105 2 0.33017394237987574 1105 3 0.20106578930330343 1105 4 0.22365397080254468 1105 5 0.28409444676104556 1105 6 0.21682310122206871 1105 7 0.3099349392294499 1105 8 0.35732526573651313 1105 9 0.33527201128352146 1105 10 0.39304802439349373 1105 11 0.351509919619609 1105 12 0.2386880975858503 1105 13 0.4573610667934389 1105 14 0.3566553049400909 1105 15 0.43849983359416456 1105 16 0.35835277479138483 1105 17 0.30787286299569416 1105 18 0.28656961523274305 1105 19 0.34370406219474603 1105 20 0.31243764612518987 1105 21 0.30119419707396133 1105 22 0.2853472064677476 1105 23 0.1991898201093889 1105 24 0.22565055839198322 1105 25 0.27326616544628024 1106 1 0.21229855944349652 1106 2 0.2245560095685697 1106 3 0.21840603025538385 1106 4 0.2613510666003691 1106 5 0.20434622108579248 1106 6 0.27410944040523333 1106 7 0.2237803626411079 1106 8 0.30592134110123936 1106 9 0.3307349765091985 1106 10 0.2761908047333179 1106 11 0.2761319963039526 1106 12 0.3263036774313335 1106 13 0.28126070616826127 1106 14 0.3362790398718616 1106 15 0.36560160111136497 1106 16 0.44831273247989145 1106 17 0.2905059504429417 1106 18 0.39628031215050186 1106 19 0.3589058272682166 1106 20 0.36562235394199405 1106 21 0.3125984009930107 1106 22 0.3819937065183723 1106 23 0.20231786822998288 1106 24 0.3960676338020974 1106 25 0.4252542819215819 1107 1 0.21101951118296114 1107 2 0.3193169169732393 1107 3 0.28902918256791565 1107 4 0.30608447966682206 1107 5 0.1779003558155381 1107 6 0.2027210775398629 1107 7 0.2516760932404125 1107 8 0.21037054340494166 1107 9 0.3728316783810044 1107 10 0.3166253677924047 1107 11 0.4091033786216727 1107 12 0.3751421075004586 1107 13 0.33946787011410606 1107 14 0.37219693343119786 1107 15 0.3144301038248898 1107 16 0.3526676526353896 1107 17 0.3349495845538897 1107 18 0.4587303114156498 1107 19 0.4459942475625027 1107 20 0.35780345220145904 1107 21 0.3976146937801275 1107 22 0.47093199802046826 1107 23 0.4530372548857855 1107 24 0.40058874010882306 1107 25 0.3887920476065929 1108 1 0.23356097171361156 1108 2 0.32543615916215884 1108 3 0.2943203954909315 1108 4 0.38338626659688657 1108 5 0.2886333151552023 1108 6 0.2188214806372244 1108 7 0.22651543682421504 1108 8 0.3384868216347243 1108 9 0.30970157567441703 1108 10 0.3248025981010443 1108 11 0.3717881542078132 1108 12 0.46642683469211477 1108 13 0.43589064246688736 1108 14 0.4505274428470756 1108 15 0.41218386057656176 1108 16 0.4133550825862571 1108 17 0.44480305134595 1108 18 0.43199248872797824 1108 19 0.3684829412560404 1108 20 0.45176725735577605 1108 21 0.43219557491298133 1108 22 0.3364164847604627 1108 23 0.29998206044827924 1108 24 0.3697104995175735 1108 25 0.4068510427228773 1109 1 0.1684174299836556 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10 -0.6235790395387681 2214 11 -0.6111818067180224 2214 12 -0.7108146372444892 2214 13 -0.7417130618396541 2214 14 -0.8004635160726252 2214 15 -0.6717375906696077 2214 16 -0.6798838040447492 2214 17 -0.7540012323326764 2214 18 -0.6755664345706265 2214 19 -0.663831131933371 2214 20 -0.6036956112050547 2214 21 -0.53099065696167 2214 22 -0.712268943762183 2214 23 -0.649579890523782 2214 24 -0.7485551239065558 2214 25 -0.7540028470686124 2215 1 -0.43163086070242684 2215 2 -0.39210631590304246 2215 3 -0.39599752310035186 2215 4 -0.2592108760905347 2215 5 -0.5012720028669406 2215 6 -0.6433913709571613 2215 7 -0.6547633409524707 2215 8 -0.6057362681484051 2215 9 -0.7853753186317078 2215 10 -0.7758994838508428 2215 11 -0.8533237697886591 2215 12 -0.9033597442500678 2215 13 -0.9319256326543908 2215 14 -0.9322599418015471 2215 15 -0.9923565438590529 2215 16 -1.0864613795452271 2215 17 -1.0385036834645263 2215 18 -1.1164665930543904 2215 19 -0.9794422933531024 2215 20 -1.031219898341753 2215 21 -1.1130084999749386 2215 22 -1.1709456159842162 2215 23 -1.0773856245600655 2215 24 -1.1183801250332692 2215 25 -1.1842296865326034 2216 1 -0.23430144166928102 2216 2 -0.1828614842913332 2216 3 -0.21271963402169547 2216 4 0.02497591612402772 2216 5 -0.21241190434089674 2216 6 -0.29971236932163947 2216 7 -0.4524981254653824 2216 8 -0.3740203707344705 2216 9 -0.47411444297084415 2216 10 -0.4221157984924844 2216 11 -0.3378307287713085 2216 12 -0.39490770731092084 2216 13 -0.4801333822200249 2216 14 -0.7243843096957718 2216 15 -0.5875790506463829 2216 16 -0.5752239046701888 2216 17 -0.6620169203633921 2216 18 -0.6145102431260057 2216 19 -0.5433621763556431 2216 20 -0.5419511653465037 2216 21 -0.7487969540660515 2216 22 -0.5698789004948921 2216 23 -0.5397398396333557 2216 24 -0.5517284241527065 2216 25 -0.6469662546946182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59939.0 3 0.0 4 1023.0 5 8130.0 6 8932.0 7 6905.0 8 5545.0 9 4863.0 10 4544.0 11 4051.0 12 3797.0 13 3685.0 14 3523.0 15 3485.0 16 3698.0 17 3796.0 18 4290.0 19 5148.0 20 6655.0 21 8722.0 22 10883.0 23 13086.0 24 15160.0 25 17201.0 26 19120.0 27 20986.0 28 24006.0 29 28854.0 30 37054.0 31 46716.0 32 59603.0 33 77802.0 34 102965.0 35 139628.0 36 199779.0 37 322957.0 38 1222908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.935359455725816 19.237489120106044 13.12793956767893 27.69921185648921 2 13.924669110716744 20.647108686633274 39.39486446601199 26.033357736637996 3 16.786574374992778 25.044763640406792 29.906216536862406 28.262445447738028 4 11.28509381397245 17.347027015825812 37.55930247339882 33.808576696802916 5 12.737622946929363 38.176893615035574 35.95253184625098 13.132951591784087 6 31.893920460339135 37.556787622987365 17.909999540140717 12.639292376532781 7 27.714494364286047 31.480233943118275 22.720162765222966 18.085108927372715 8 25.293040132448958 36.55068348796075 20.535527105564594 17.620749274025705 9 26.46091358258883 14.561353309268055 19.815039019067576 39.162694089075536 10 14.374173576279656 28.98363133073447 34.96747706337928 21.674718029606595 11 34.417254214985896 22.122235288444948 24.521018442767488 18.939492053801665 12 22.85873456178851 24.74039018282572 31.849349595666602 20.551525659719164 13 28.889006666430227 21.576176986171014 25.689845419633635 23.84497092776513 14 21.048875166225457 21.522176877037595 27.63560749551784 29.79334046121911 15 23.337646382318916 30.23775433473378 23.144734739517478 23.279864543429827 16 22.58727946764197 27.784457004135188 27.06146672622845 22.56679680199439 17 22.101916743294147 28.188550239926723 27.36679273830549 22.342740278473634 18 22.012604187843056 27.349652607536207 28.763896695087876 21.873846509532864 19 23.512785128468952 26.899677577339 27.33451580213745 22.253021492054597 20 23.824926607102228 26.966146616833488 27.454980973835184 21.753945802229104 21 23.323560787770518 26.47792649350784 26.909740971088436 23.28877174763321 22 23.40881513169299 27.4539886468468 26.708960383512625 22.428235837947586 23 22.590374169585818 27.2229196306525 27.1325354980891 23.054170701672586 24 23.10022279880085 26.859549086469126 27.594374678962108 22.445853435767916 25 22.984036632976455 26.87280353769158 27.186699146779368 22.956460682552592 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 308.5 3 431.0 4 431.0 5 431.0 6 1005.5 7 1580.0 8 1580.0 9 1580.0 10 2331.5 11 3083.0 12 3083.0 13 3083.0 14 5835.5 15 8588.0 16 8588.0 17 8588.0 18 16864.0 19 25140.0 20 25140.0 21 25140.0 22 41114.5 23 57089.0 24 57089.0 25 57089.0 26 83463.0 27 109837.0 28 109837.0 29 109837.0 30 142021.0 31 174205.0 32 174205.0 33 174205.0 34 202899.0 35 231593.0 36 231593.0 37 231593.0 38 255047.5 39 278502.0 40 278502.0 41 278502.0 42 295577.5 43 312653.0 44 312653.0 45 312653.0 46 325078.5 47 337504.0 48 337504.0 49 337504.0 50 327085.0 51 316666.0 52 316666.0 53 316666.0 54 283349.0 55 250032.0 56 250032.0 57 250032.0 58 214889.0 59 179746.0 60 179746.0 61 179746.0 62 146210.0 63 112674.0 64 112674.0 65 112674.0 66 87313.0 67 61952.0 68 61952.0 69 61952.0 70 45649.0 71 29346.0 72 29346.0 73 29346.0 74 20604.5 75 11863.0 76 11863.0 77 11863.0 78 8262.0 79 4661.0 80 4661.0 81 4661.0 82 3082.0 83 1503.0 84 1503.0 85 1503.0 86 983.5 87 464.0 88 464.0 89 464.0 90 290.5 91 117.0 92 117.0 93 117.0 94 70.5 95 24.0 96 24.0 97 24.0 98 12.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19203495283208716 2 0.00860750151727139 3 0.00589773252109336 4 0.002311273555563614 5 0.08599531608459102 6 0.34565494518894463 7 0.03168038752884609 8 0.015820268992392323 9 0.006096980241400568 10 0.005299989360171735 11 0.0 12 0.0 13 0.0 14 0.0018330790268263144 15 0.0 16 0.0 17 0.021837550145670008 18 0.029847308502019776 19 0.0 20 0.0 21 0.0011157872337203654 22 1.5939817624576648E-4 23 0.005937582065154801 24 0.0 25 2.3909726436864974E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2509439.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.35929143284852 #Duplication Level Percentage of deduplicated Percentage of total 1 82.28452568934468 58.717654490796576 2 11.910922361180242 16.99909960010986 3 3.067588449695208 6.567028145335209 4 1.1423267092765093 3.26062498235157 5 0.5100223750354002 1.8197417648712337 6 0.2873534881206677 1.230320478182898 7 0.17133124614987857 0.8558253427893603 8 0.11715096412364709 0.668784783243081 9 0.09343802933930155 0.6000904409880843 >10 0.3818005263747818 4.578507295093768 >50 0.019035185351172854 0.9159193474285501 >100 0.01176724713209912 1.556760249275293 >500 0.0018437765902116444 0.9120632850188576 >1k 8.939522861364793E-4 1.3175797945157388 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3253 0.1296305668318696 No Hit GTACATGGGAAGCAGTGGTATCAAC 3131 0.12476892245637372 No Hit CCCATGTACTCTGCGTTGATACCAC 3106 0.12377268385483768 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2802 0.11165842246015942 No Hit GTCCTACAGTGGACATTTCTAAATT 2738 0.10910805164022716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.984954406144162E-5 0.0 5 0.0 0.0 0.0 3.984954406144162E-5 0.0 6 0.0 0.0 0.0 3.984954406144162E-5 0.0 7 0.0 0.0 0.0 3.984954406144162E-5 0.0 8 0.0 0.0 0.0 3.984954406144162E-5 0.0 9 0.0 0.0 0.0 3.984954406144162E-5 0.0 10 0.0 0.0 0.0 3.984954406144162E-5 0.0 11 0.0 0.0 0.0 7.969908812288324E-5 0.0 12 0.0 0.0 0.0 7.969908812288324E-5 0.0 13 0.0 0.0 0.0 7.969908812288324E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 645 0.0 12.104824 4 ACCGTGC 75 2.0971675E-4 11.386835 8 GCGCCAC 155 3.1832315E-10 11.021275 13 GGCGAGG 780 0.0 10.703865 19 GTCCTAC 1660 0.0 10.6735525 1 GGACGTG 1420 0.0 10.660521 6 GTCCTAA 845 0.0 10.596812 1 TGGCGAG 1685 0.0 10.531691 18 AGGACGT 1500 0.0 10.410148 5 TCCTACA 1830 0.0 10.251331 2 GTAGGAC 1530 0.0 10.206844 3 AATACCG 75 0.002599382 10.156242 5 GGACCGT 75 0.0026011076 10.15543 6 ACCCGTC 75 0.0026687484 10.124052 7 TATGGCG 1795 0.0 10.045699 16 GACGTGA 880 0.0 9.922722 7 CCTACAG 1875 0.0 9.903128 3 GTGCGCC 125 2.6089365E-6 9.868787 11 TGTAGGA 1720 0.0 9.799636 2 ATGGCGA 1895 0.0 9.765993 17 >>END_MODULE