FastQCFastQC Report
Thu 26 May 2016
SRR1513144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40040.1749766748750713No Hit
CCCATGTACTCTGCGTTGATACCAC33470.14626546723448142No Hit
GTCCTACAGTGGACATTTCTAAATT27160.11869047176840498No Hit
GTCCTAAAGTGTGTATTTCTCATTT25300.11056218467380878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG400.005243292514.2650375
TAGGACC7700.013.1161494
CGAAATC1201.0184522E-811.8620613
GGCGAGG6550.011.156013519
GTCTTAC2500.011.0784781
GCCGCGC600.005934629611.0688348
GCACCGA600.005934629611.0688346
ACGAAAT1303.3202014E-810.94983212
AGGACCT13900.010.9467915
CGCAGAT1658.185452E-1110.9250848
GTATAAA3650.010.72785951
AACCGTG803.8180375E-410.673527
GGACCTG13300.010.3435956
GTATAAG3150.010.3083811
ATTAGAC1855.638867E-1110.3007433
GTAGGAC17050.010.2826193
TGGCGAG15150.010.27284618
GTCCTAC16150.010.2304721
CAGATTA2053.6379788E-1210.18356610
TGTAGGA17800.010.1776872
Produced by FastQC (version 0.11.5)