##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513144_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2288305
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.524676998914043	31.0	30.0	34.0	25.0	34.0
2	29.375862046361828	31.0	30.0	34.0	23.0	34.0
3	29.565546550831293	31.0	30.0	34.0	24.0	34.0
4	32.63826238198142	37.0	33.0	37.0	23.0	37.0
5	32.793497807329004	37.0	33.0	37.0	25.0	37.0
6	33.17594944729833	37.0	35.0	37.0	27.0	37.0
7	33.12730296005122	37.0	35.0	37.0	27.0	37.0
8	33.013943508404694	37.0	35.0	37.0	26.0	37.0
9	34.27388744070393	39.0	35.0	39.0	25.0	39.0
10	33.85201360832581	39.0	34.0	39.0	20.0	39.0
11	34.31129329350764	39.0	35.0	39.0	27.0	39.0
12	34.30601034390084	39.0	35.0	39.0	26.0	39.0
13	34.343943661356334	39.0	35.0	39.0	26.0	39.0
14	34.578204828464735	40.0	34.0	41.0	17.0	41.0
15	35.016412584860845	40.0	34.0	41.0	23.0	41.0
16	34.863939029106696	40.0	34.0	41.0	19.0	41.0
17	34.47347796731642	39.0	34.0	41.0	17.0	41.0
18	34.20987761683867	39.0	33.0	41.0	17.0	41.0
19	34.8437559678452	40.0	34.0	41.0	21.0	41.0
20	35.06037175988341	40.0	35.0	41.0	24.0	41.0
21	35.10272800173054	40.0	36.0	41.0	24.0	41.0
22	34.79689158569334	40.0	35.0	41.0	18.0	41.0
23	34.333088465042906	40.0	34.0	41.0	14.0	41.0
24	34.589101540223005	40.0	34.0	41.0	18.0	41.0
25	34.23056410749441	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8954769763772674
1101	2	1.1228216803149813
1101	3	1.1183266190087053
1101	4	1.4059982304022753
1101	5	1.2474495675075232
1101	6	1.047905452990591
1101	7	0.9642686170380159
1101	8	1.0035405416229608
1101	9	1.365574375414269
1101	10	1.726187963668842
1101	11	1.3252384491236384
1101	12	1.2088772512279036
1101	13	1.0559536232573734
1101	14	1.9893462779028255
1101	15	1.7003302760326804
1101	16	1.7656400688619698
1101	17	2.1323802144455826
1101	18	2.1370912483717746
1101	19	1.6613546525135234
1101	20	1.3270324408276863
1101	21	1.3532985619033013
1101	22	1.6602975230035568
1101	23	1.9113933427768544
1101	24	1.6194577456526318
1101	25	1.9172930789825529
1102	1	0.9181171926933729
1102	2	1.2276233789901987
1102	3	1.1373805273906754
1102	4	1.3616174962982157
1102	5	1.3705827104035109
1102	6	1.0485637520678637
1102	7	0.9637816560767476
1102	8	1.127915365602533
1102	9	1.3830113236812522
1102	10	1.612918901783182
1102	11	1.1684977162959527
1102	12	1.0803046434062864
1102	13	1.0375476088039335
1102	14	2.1436882771064845
1102	15	1.74972004874261
1102	16	1.8387136943071383
1102	17	2.256877819180339
1102	18	2.3770690091005235
1102	19	1.838318832442134
1102	20	1.6338251300314468
1102	21	1.5166469110223986
1102	22	1.7751818108127466
1102	23	1.9605569769207207
1102	24	1.7446337660855633
1102	25	2.1023975536127253
1103	1	0.8039148250252097
1103	2	1.0215949182399449
1103	3	0.7843392849621331
1103	4	1.2274998009955453
1103	5	1.178966349964199
1103	6	0.916636542873249
1103	7	0.7868188454211165
1103	8	0.8096362112167768
1103	9	1.1584374505220296
1103	10	1.4577635386612116
1103	11	1.0449485826044054
1103	12	1.0590985224664706
1103	13	1.0321968400621557
1103	14	1.8057144856895633
1103	15	1.451777950333998
1103	16	1.466575312961261
1103	17	1.8823139681310153
1103	18	2.0175299853186814
1103	19	1.3763867704364117
1103	20	1.0608499620632657
1103	21	1.1015329907711688
1103	22	1.3161818540320311
1103	23	1.7611244767667316
1103	24	1.5143760464235498
1103	25	1.713470606796605
1104	1	0.7978092515697028
1104	2	1.0124434059407719
1104	3	0.8562674673419544
1104	4	1.1933965911806084
1104	5	1.2203943056168
1104	6	0.9800551709606822
1104	7	0.8641070534394188
1104	8	0.870561213439224
1104	9	1.0880753169803228
1104	10	1.4394590199790613
1104	11	1.0255628452777898
1104	12	0.8934076170292755
1104	13	0.8450038275932812
1104	14	1.942133550298479
1104	15	1.5435590202424265
1104	16	1.6820202050498096
1104	17	1.948817132226985
1104	18	2.0987979345566856
1104	19	1.4881315390887409
1104	20	1.3782927181100106
1104	21	1.2514752568829337
1104	22	1.3963013508544861
1104	23	1.7751554177481808
1104	24	1.418934969228971
1104	25	1.722872378132557
1105	1	0.5536937889956519
1105	2	0.8293291166327528
1105	3	0.7570096560501476
1105	4	1.092090574944585
1105	5	1.0777667258233734
1105	6	0.7384262075793657
1105	7	0.757933859578344
1105	8	0.7570900986715401
1105	9	0.8824433925972173
1105	10	1.2637346158394323
1105	11	0.8744928637737388
1105	12	0.6721592537250771
1105	13	0.6671244079991183
1105	14	1.48249509768997
1105	15	1.1157022617970753
1105	16	1.24887042908383
1105	17	1.527339146945394
1105	18	1.244931785859137
1105	19	1.1014011985116738
1105	20	0.860468435833333
1105	21	0.8309116355530008
1105	22	1.0309045745524017
1105	23	1.3636903198351078
1105	24	1.1814114884435227
1105	25	1.4336117831691482
1106	1	0.4841955762450958
1106	2	0.5717201232099924
1106	3	0.5415424826190787
1106	4	1.0296774385583234
1106	5	0.8969988461305576
1106	6	0.5911175296503544
1106	7	0.5413423437686902
1106	8	0.5220316274382384
1106	9	0.7697533867973974
1106	10	0.6207622287405385
1106	11	0.5443636635318754
1106	12	0.5480580818324015
1106	13	0.49970812490209937
1106	14	1.4815885981897452
1106	15	0.9339906979435284
1106	16	0.9810433048483986
1106	17	1.337012917363019
1106	18	1.443539088696113
1106	19	0.9837615195173015
1106	20	0.7727416772744888
1106	21	0.509747887306311
1106	22	0.8522513667584306
1106	23	1.2536037338723176
1106	24	0.8599328223198128
1106	25	1.171447586567595
1107	1	0.7536729608744324
1107	2	0.8808440573519327
1107	3	0.931799656111167
1107	4	1.1738853732026726
1107	5	1.022128630701502
1107	6	0.8073040722087796
1107	7	0.8109079891805138
1107	8	0.863634074686658
1107	9	1.1266351333521953
1107	10	1.2998094099481676
1107	11	0.9369985437081638
1107	12	0.8620700629911511
1107	13	0.7469442772945243
1107	14	1.659805788577465
1107	15	1.1974728961801588
1107	16	1.3510169866469397
1107	17	1.838322523094348
1107	18	1.9473958909109754
1107	19	1.5134278113181168
1107	20	1.2712829496167117
1107	21	1.0982382933107644
1107	22	1.3040961383497063
1107	23	1.546882432811394
1107	24	1.323634605624079
1107	25	1.6235280435450719
1108	1	0.7834018961470548
1108	2	1.0153259026537533
1108	3	0.8058868349891313
1108	4	1.2797295071312362
1108	5	1.2091221681678448
1108	6	0.9035372789124025
1108	7	0.86234505581006
1108	8	0.8711895711204178
1108	9	1.1629633411896378
1108	10	1.388748583977197
1108	11	0.890637335951098
1108	12	0.8260676573348746
1108	13	0.7874042132295784
1108	14	1.7339271284701567
1108	15	1.2358909171350376
1108	16	1.3224851096066743
1108	17	1.6407370950671876
1108	18	1.9466406653309036
1108	19	1.3058187100713354
1108	20	1.074060630570699
1108	21	1.0326237732512809
1108	22	1.3399958712662183
1108	23	1.6156447931588858
1108	24	1.298216897904041
1108	25	1.6521804563267892
1109	1	0.4794147930706565
1109	2	0.6885951357240927
1109	3	0.6593997128762794
1109	4	0.9601754822272071
1109	5	1.0105904768281277
1109	6	0.6953932676167156
1109	7	0.6116404351738964
1109	8	0.6004711137038754
1109	9	0.9509799496772899
1109	10	1.1365330130305225
1109	11	0.6836572676591928
1109	12	0.6228078554662559
1109	13	0.6106132367249089
1109	14	1.569318392805961
1109	15	1.128654781382302
1109	16	1.3021525482467524
1109	17	1.5670638541168458
1109	18	1.7709799101168215
1109	19	1.1863524704013315
1109	20	0.9677648954971758
1109	21	0.828422482906241
1109	22	1.1105109106064006
1109	23	1.4633689582473366
1109	24	1.230853424611098
1109	25	1.4183715017978002
1110	1	0.08226667963711165
1110	2	0.36392630917160673
1110	3	0.20977226604583166
1110	4	0.5345340093184276
1110	5	0.5092279501493522
1110	6	0.13513431389765884
1110	7	0.1828434047455545
1110	8	0.25298727214615013
1110	9	0.3649992056873259
1110	10	0.7307055077451565
1110	11	0.19111267252924335
1110	12	0.1654628869691095
1110	13	0.13407683768716794
1110	14	0.809027110573389
1110	15	0.5434995386199688
1110	16	0.7279011626559679
1110	17	0.9731856496595412
1110	18	0.5317078210978892
1110	19	0.18937165896339536
1110	20	0.12306619026577437
1110	21	-0.038064012380537804
1110	22	0.3546668534322279
1110	23	-0.8498942601996049
1110	24	-0.0656270313682441
1110	25	0.3301912559925597
1111	1	0.27463135896170954
1111	2	0.6162468850894278
1111	3	0.5366146165323258
1111	4	0.9148714645264988
1111	5	0.8285616591413856
1111	6	0.4075606196861443
1111	7	0.31068144283918997
1111	8	0.385380460048097
1111	9	0.6328105942916551
1111	10	0.8073467963236354
1111	11	0.5320247088567456
1111	12	0.45604295700756126
1111	13	0.33024204608364016
1111	14	1.2198334055069466
1111	15	0.747043252348071
1111	16	0.8172005361356156
1111	17	1.266209268143946
1111	18	1.4433754038647635
1111	19	0.9021081405317304
1111	20	0.6367495007638198
1111	21	0.4106033787158907
1111	22	0.6918326737144938
1111	23	1.0470099356929623
1111	24	0.7719656493560478
1111	25	0.9905208139175627
1112	1	0.07386876782723562
1112	2	0.1880963807079894
1112	3	0.20286754508138571
1112	4	0.59591625580331
1112	5	0.2739809884082689
1112	6	0.09923199515506553
1112	7	-0.009055559230475296
1112	8	0.17647332845896813
1112	9	0.37878665681726176
1112	10	0.6663555171614277
1112	11	0.05725740863771023
1112	12	0.09976069619425232
1112	13	0.020560489438693708
1112	14	0.5547266811679492
1112	15	0.36057640776964917
1112	16	0.3135390654355774
1112	17	0.8467756748826716
1112	18	0.9027491361164763
1112	19	0.4959221487070664
1112	20	0.21356300686368002
1112	21	0.07595617470501281
1112	22	0.39590864733555264
1112	23	0.7761144604778636
1112	24	0.5825333642165944
1112	25	0.8492729919711834
1113	1	-0.036145693179054916
1113	2	0.1088329341915184
1113	3	0.035973203874839044
1113	4	0.26824496062823755
1113	5	0.3076747436987688
1113	6	-0.0037545102419187515
1113	7	-0.02679750853051388
1113	8	0.09687487252377025
1113	9	0.23315419999586595
1113	10	0.4774986686640261
1113	11	0.08313918348677163
1113	12	-0.06280717981332629
1113	13	-0.10588695449862229
1113	14	0.5204185861972164
1113	15	0.23321333371948327
1113	16	0.4008770020983974
1113	17	0.6095659560273745
1113	18	0.5256119325495874
1113	19	0.19457167605721537
1113	20	0.17235116363438152
1113	21	0.08992167543195961
1113	22	0.24604535329431343
1113	23	0.1802879832085864
1113	24	0.10741096741188016
1113	25	0.5276920621214742
1114	1	-1.024882370938709
1114	2	-0.8128665788275704
1114	3	-0.7862992962317001
1114	4	-0.4123242297902081
1114	5	-3.6265584260162136
1114	6	-1.4601985810459226
1114	7	-1.127093106331543
1114	8	-0.8760494536643364
1114	9	-0.4766272590872802
1114	10	-0.34134598171312547
1114	11	-0.7202224182426491
1114	12	-0.9037941587883012
1114	13	-0.9329440323807034
1114	14	-6.586024128653573
1114	15	-2.7312862614316273
1114	16	-1.5547228909750075
1114	17	-0.6164636967323744
1114	18	-6.6871429382280745
1114	19	-2.6945404070356034
1114	20	-1.6689042940773149
1114	21	-1.2478172144685118
1114	22	-1.0614395084565729
1114	23	-0.7434384861604286
1114	24	-0.8204496096043243
1114	25	-0.6336318905983731
1115	1	-0.7464576767653561
1115	2	-0.407078991864644
1115	3	-0.40619462562461806
1115	4	-3.1446699681488646
1115	5	-1.1031291812654977
1115	6	-0.7339328830195981
1115	7	-0.567677954447781
1115	8	-0.46853968201391893
1115	9	-0.21304470164814404
1115	10	-0.6196974709463561
1115	11	-0.5654119963213233
1115	12	-0.6248581292073538
1115	13	-0.7471756882779133
1115	14	-5.985444497946482
1115	15	-2.3949987411518805
1115	16	-1.230820195655582
1115	17	-0.4867870441986355
1115	18	-0.15135722718753897
1115	19	-0.4937178590347031
1115	20	-0.5642428467738796
1115	21	-0.6799039143253296
1115	22	-1.830828412082525
1115	23	-0.9101288461176935
1115	24	-0.6193386385360355
1115	25	-0.27529422163712525
1116	1	-0.2945314743399656
1116	2	-0.0228431325301095
1116	3	-0.02775880558211341
1116	4	0.08658505497984947
1116	5	-0.599716132739843
1116	6	-0.3239384196236941
1116	7	-0.2324222883585918
1116	8	-0.1316739357332679
1116	9	-0.08120119108394874
1116	10	0.17878174892826593
1116	11	-0.19408714054092968
1116	12	-0.45500047351269046
1116	13	-0.39825711207816994
1116	14	0.5242213412062426
1116	15	0.13192880077450297
1116	16	0.08205062286882736
1116	17	0.45017782515536453
1116	18	0.2849771836999224
1116	19	0.028850946666210575
1116	20	-0.29345200538865157
1116	21	-0.3138316884318826
1116	22	-0.33491835510908174
1116	23	-1.034845318407882
1116	24	-0.7025707651278097
1116	25	-0.09812824853430158
1201	1	0.9667006309717934
1201	2	1.126556638025722
1201	3	1.0451875683982053
1201	4	1.5194109232709962
1201	5	1.3228511339599365
1201	6	1.0936347693804649
1201	7	1.0104782233877856
1201	8	1.1108888317631767
1201	9	1.3773472002418572
1201	10	1.7749452660892686
1201	11	1.281128595118716
1201	12	1.2796213901074935
1201	13	1.2069641677855785
1201	14	2.043017713115553
1201	15	1.6921927033697912
1201	16	1.7876258729945107
1201	17	2.1940415996145575
1201	18	1.5963054860828692
1201	19	1.5493902278652811
1201	20	1.4235214291716858
1201	21	1.4659775286269365
1201	22	1.6916812845746492
1201	23	1.9016326945858495
1201	24	1.5997889986184148
1201	25	1.8587662675676384
1202	1	0.8790664899675704
1202	2	1.0414017204750898
1202	3	0.9444509467296562
1202	4	1.3557649017450686
1202	5	1.1596227119798073
1202	6	0.9479687002114545
1202	7	0.8901559639416874
1202	8	1.091199636713263
1202	9	1.2356999790019856
1202	10	1.5901782796421315
1202	11	1.174386915574793
1202	12	1.0543719363110142
1202	13	1.0881804374166961
1202	14	1.9992659030751483
1202	15	1.621490976878782
1202	16	1.6061959754117225
1202	17	2.0189637483917267
1202	18	2.065354205945617
1202	19	1.6835505700812021
1202	20	1.3676128885279013
1202	21	1.283240062490549
1202	22	1.5168437468918938
1202	23	1.7643505514070057
1202	24	1.4187321262957724
1202	25	1.7982158501324008
1203	1	0.7185932540389999
1203	2	0.8062492503615495
1203	3	0.6758629709781516
1203	4	1.067317418534536
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2211	14	-0.016767387641621667
2211	15	-0.38622155611342635
2211	16	-7.109453317900307
2211	17	-2.1734364607600796
2211	18	-0.7807258669164554
2211	19	-0.700056776620265
2211	20	-0.7940061213251468
2211	21	-0.7594052419336563
2211	22	-0.6339640423236901
2211	23	-0.17328331587830803
2211	24	-0.7309625724296325
2211	25	-7.886772175518235
2212	1	-1.8713316922515588
2212	2	-4.278862301940769
2212	3	-2.1154951779498994
2212	4	-3.943647128690113
2212	5	-1.6884552389334395
2212	6	-1.5395985456322094
2212	7	-1.3422703035329562
2212	8	-1.261232522883013
2212	9	-1.385133814407581
2212	10	-0.8718049525058902
2212	11	-1.3934280856473578
2212	12	-1.391204584040402
2212	13	-1.6719498907773058
2212	14	-7.2998694524544625
2212	15	-5.809133773618331
2212	16	-3.5713111884078153
2212	17	-8.114024236776356
2212	18	-3.5974252490787464
2212	19	-2.50813685113566
2212	20	-2.4439681976672603
2212	21	-1.9993456037045618
2212	22	-1.9947106155588372
2212	23	-1.4505886508947228
2212	24	-1.766618118338954
2212	25	-1.6067217824049465
2213	1	-0.4619473776109153
2213	2	-0.35285398487289754
2213	3	-0.4664780648659743
2213	4	-0.11320782561188025
2213	5	-0.24804494694194545
2213	6	-0.4768250392456892
2213	7	-0.5764728369214822
2213	8	-0.5618728739249121
2213	9	-0.36490250061426366
2213	10	-4.315792901013147
2213	11	-1.8167338623227849
2213	12	-1.2111084427176024
2213	13	-0.9663351546711993
2213	14	-0.037530203351039404
2213	15	-0.6801769001157894
2213	16	-0.42488462767602186
2213	17	-0.07381792246891905
2213	18	0.012871347110710474
2213	19	-0.5529952514606435
2213	20	-0.837789378621757
2213	21	-0.8175803711703011
2213	22	-0.9744363096835116
2213	23	-1.19690161417725
2213	24	-0.8138810203596236
2213	25	-0.4570538273169902
2214	1	-1.0691175960032027
2214	2	-0.7563117457448065
2214	3	-0.6470315448800967
2214	4	-0.21119183312787726
2214	5	-3.692914378510647
2214	6	-1.4749529131930927
2214	7	-1.1379831639534004
2214	8	-0.8997199740474002
2214	9	-0.7870327437090694
2214	10	-4.434406139769788
2214	11	-1.9498805225684563
2214	12	-1.36860264512066
2214	13	-1.0722842895436528
2214	14	-0.3331562085720918
2214	15	-0.7092918562382309
2214	16	-0.6185653837338094
2214	17	-0.3033798504416936
2214	18	-0.0017510578281374478
2214	19	-0.8190020363747834
2214	20	-1.0309597362539265
2214	21	-1.1462289919342155
2214	22	-0.8342210820728653
2214	23	-0.6251309189105356
2214	24	-1.196389239134156
2214	25	-0.9520335052412463
2215	1	-2.233668295474704
2215	2	-4.7360587368775455
2215	3	-2.939484545140626
2215	4	-1.9101947605801008
2215	5	-1.810823419301908
2215	6	-2.0800909872854305
2215	7	-2.2182655171271897
2215	8	-2.4918040187931076
2215	9	-8.138076949749596
2215	10	-3.8211584007817514
2215	11	-3.026740391001752
2215	12	-3.3502252352354773
2215	13	-2.813589744816557
2215	14	-8.087917098415215
2215	15	-4.797652907515747
2215	16	-9.344734910782211
2215	17	-4.595463429958603
2215	18	-2.9776293807248386
2215	19	-3.56263029181331
2215	20	-3.4047087069352067
2215	21	-3.4905147319736685
2215	22	-9.381367659384587
2215	23	-10.345638320700736
2215	24	-6.61626201068821
2215	25	-5.0190790813805926
2216	1	-1.4954463239205147
2216	2	-1.4939747378142343
2216	3	-1.368180522973777
2216	4	-6.47930292424099
2216	5	-2.976236823611579
2216	6	-2.1324534276778557
2216	7	-1.6576582957589956
2216	8	-7.517649631249746
2216	9	-3.4619191958347706
2216	10	-1.6942640115129066
2216	11	-1.5943151581736643
2216	12	-1.8876829104951938
2216	13	-1.6637990996205687
2216	14	-0.9529383616252431
2216	15	-1.5811169732230184
2216	16	-1.327746611570106
2216	17	-1.0118630343301263
2216	18	-7.077996928097175
2216	19	-4.202464469027081
2216	20	-3.5369046830876947
2216	21	-2.5737656893923457
2216	22	-2.2265242357136543
2216	23	-1.4713231915808649
2216	24	-1.7174034341440105
2216	25	-1.2348564056278803
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101845.0
3	6.0
4	892.0
5	5081.0
6	6089.0
7	5519.0
8	4928.0
9	4603.0
10	4489.0
11	4313.0
12	4274.0
13	4252.0
14	4133.0
15	4284.0
16	4326.0
17	4637.0
18	5267.0
19	6456.0
20	8231.0
21	10405.0
22	12793.0
23	15474.0
24	17905.0
25	20815.0
26	23434.0
27	26084.0
28	29421.0
29	34857.0
30	42196.0
31	50869.0
32	65236.0
33	84695.0
34	112022.0
35	152402.0
36	217904.0
37	320189.0
38	867979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41354468940297	18.552474738697693	12.38181370087764	25.6521668710217
2	14.29650494633493	20.365825927741756	41.363969546051806	23.973699579871514
3	18.366813133948558	25.046168082049718	29.755465797063728	26.83155298693799
4	11.39584956597127	16.978325998924532	38.96583738463396	32.65998705047025
5	12.644730132081852	38.04862511850916	36.2801880725022	13.026456676906792
6	31.71543738919718	37.091013039327784	18.402009224389033	12.791540347085995
7	27.74546489250059	31.46450873802873	22.738849577751914	18.05117679171877
8	25.090826124355736	36.46668892882868	21.054221185652445	17.388263761163135
9	26.663997510341524	14.787635978362351	20.181400642695547	38.36696586860058
10	13.95544719341971	28.778408271345164	35.713405268462914	21.55273926677221
11	34.32827535563564	22.25399454936063	24.653499751945642	18.764230343058085
12	23.14374402492712	24.62226173911941	31.997889517924456	20.236104718029015
13	28.929618665776086	21.74692654302315	25.70727540727348	23.616179383927275
14	20.518232965292167	21.645786133390917	28.76683406937195	29.069146831944963
15	23.401979150499816	30.22792801260851	23.37640364702308	22.9936891898686
16	22.197472497499774	27.64573877345493	27.926096428346632	22.230692300698664
17	21.719884318007438	28.027700722028765	28.242188951626478	22.010226008337323
18	21.589789882996328	27.177026799589953	29.75661574577001	21.476567571643713
19	23.544343637233926	26.87772796915605	27.41220775076553	22.165720642844498
20	23.84934531508938	27.064783053249514	27.581527975523574	21.504343656137532
21	23.358178614988482	26.540312744536585	26.924970163803998	23.176538476670935
22	23.347469483403867	27.493616872442377	26.994552481550137	22.164361162603615
23	22.329870453467507	27.35265639241212	27.708488609216232	22.608984544904146
24	23.23369583038758	26.980912394286268	27.59651467673666	22.188877098589494
25	22.91805923202525	26.84982589745327	27.51655231054736	22.71556255997412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	238.0
1	238.0
2	341.0
3	444.0
4	444.0
5	444.0
6	931.0
7	1418.0
8	1418.0
9	1418.0
10	2319.5
11	3221.0
12	3221.0
13	3221.0
14	6157.0
15	9093.0
16	9093.0
17	9093.0
18	17204.5
19	25316.0
20	25316.0
21	25316.0
22	40901.0
23	56486.0
24	56486.0
25	56486.0
26	81173.5
27	105861.0
28	105861.0
29	105861.0
30	136126.0
31	166391.0
32	166391.0
33	166391.0
34	191409.5
35	216428.0
36	216428.0
37	216428.0
38	237118.5
39	257809.0
40	257809.0
41	257809.0
42	271532.0
43	285255.0
44	285255.0
45	285255.0
46	295441.5
47	305628.0
48	305628.0
49	305628.0
50	293418.0
51	281208.0
52	281208.0
53	281208.0
54	250653.5
55	220099.0
56	220099.0
57	220099.0
58	188699.5
59	157300.0
60	157300.0
61	157300.0
62	128543.5
63	99787.0
64	99787.0
65	99787.0
66	77125.0
67	54463.0
68	54463.0
69	54463.0
70	39926.5
71	25390.0
72	25390.0
73	25390.0
74	17865.0
75	10340.0
76	10340.0
77	10340.0
78	7155.0
79	3970.0
80	3970.0
81	3970.0
82	2733.5
83	1497.0
84	1497.0
85	1497.0
86	984.0
87	471.0
88	471.0
89	471.0
90	307.0
91	143.0
92	143.0
93	143.0
94	89.5
95	36.0
96	36.0
97	36.0
98	24.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1696452177485082
2	0.25818236642405623
3	0.2571335551860438
4	0.447055790202792
5	0.2526324069562405
6	0.019971113990486407
7	0.018878602284223476
8	0.018223095260465717
9	0.019271906498478132
10	0.021981335530010204
11	0.021850234125258654
12	0.0295415165373497
13	0.0285364057675878
14	0.026569884696314522
15	0.041952449520496614
16	0.022418340212515375
17	0.04370046825051731
18	0.03364936055289832
19	0.028842309045341425
20	0.03128953526737039
21	0.0356158816241716
22	0.04479297995678024
23	0.031027332457867287
24	0.048682321631076275
25	0.03758240269544488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2288305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.66981987432199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34397371731038	56.672199967465964
2	12.304922243916385	17.145634326023853
3	3.30704417533526	6.9120351603609995
4	1.2296581419207495	3.426802450184484
5	0.5755841694784932	2.005042270503892
6	0.33557730512381795	1.4027766241132074
7	0.20768837202774054	1.01287280284147
8	0.12704273329412263	0.7080835475954443
9	0.09247160018780556	0.579823175571726
>10	0.43947299827485736	5.179073404474524
>50	0.022439142469354228	1.0646996501384227
>100	0.011264671151813857	1.6414504821791136
>500	0.001780009472413492	0.822028626333589
>1k	0.0010807200368198305	1.427477512213423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4004	0.1749766748750713	No Hit
CCCATGTACTCTGCGTTGATACCAC	3347	0.14626546723448142	No Hit
GTCCTACAGTGGACATTTCTAAATT	2716	0.11869047176840498	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2530	0.11056218467380878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.3700468250517304E-5	0.0
3	0.0	0.0	0.0	8.740093650103461E-5	0.0
4	0.0	0.0	0.0	8.740093650103461E-5	0.0
5	0.0	0.0	0.0	8.740093650103461E-5	4.3700468250517304E-5
6	0.0	0.0	0.0	8.740093650103461E-5	4.3700468250517304E-5
7	0.0	0.0	0.0	8.740093650103461E-5	4.3700468250517304E-5
8	0.0	0.0	0.0	1.311014047515519E-4	4.3700468250517304E-5
9	0.0	0.0	0.0	1.7480187300206922E-4	8.740093650103461E-5
10	0.0	0.0	0.0	2.622028095031038E-4	8.740093650103461E-5
11	0.0	0.0	0.0	3.0590327775362116E-4	8.740093650103461E-5
12	0.0	0.0	0.0	4.3700468250517304E-4	8.740093650103461E-5
13	4.3700468250517304E-5	0.0	0.0	4.807051507556904E-4	8.740093650103461E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	40	0.0052432925	14.265037	5
TAGGACC	770	0.0	13.116149	4
CGAAATC	120	1.0184522E-8	11.86206	13
GGCGAGG	655	0.0	11.1560135	19
GTCTTAC	250	0.0	11.078478	1
GCCGCGC	60	0.0059346296	11.068834	8
GCACCGA	60	0.0059346296	11.068834	6
ACGAAAT	130	3.3202014E-8	10.949832	12
AGGACCT	1390	0.0	10.946791	5
CGCAGAT	165	8.185452E-11	10.925084	8
GTATAAA	365	0.0	10.7278595	1
AACCGTG	80	3.8180375E-4	10.67352	7
GGACCTG	1330	0.0	10.343595	6
GTATAAG	315	0.0	10.308381	1
ATTAGAC	185	5.638867E-11	10.300743	3
GTAGGAC	1705	0.0	10.282619	3
TGGCGAG	1515	0.0	10.272846	18
GTCCTAC	1615	0.0	10.230472	1
CAGATTA	205	3.6379788E-12	10.183566	10
TGTAGGA	1780	0.0	10.177687	2
>>END_MODULE