FastQCFastQC Report
Thu 26 May 2016
SRR1513144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288305
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC43590.19049034110400492No Hit
GTACATGGGAAGCAGTGGTATCAAC42920.1875624097312203No Hit
GTATCAACGCAGAGTACTTTTTTTT29400.12847937665652087No Hit
GTCCTACAGTGGACATTTCTAAATT27610.12065699283967829No Hit
GTCCTAAAGTGTGTATTTCTCATTT26030.11375231885609653No Hit
CTTTAGGACGTGAAATATGGCGAGG25350.11078068701506137No Hit
CTGTAGGACGTGGAATATGGCAAGA23470.10256499898396411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7700.012.9807354
GGCGAGG7050.012.51636419
TGGCGAG14950.011.80834318
TGTACCG1303.1726813E-810.9836985
ATGGCGA17450.010.49848417
TTAGGAC10850.010.4411053
AGGACCT13200.010.3845865
AGGACGT15650.010.340355
TATGGCG17150.010.29469516
GTAGGAC17850.010.2398193
GGACGTG15650.010.2175796
GACTGCG750.002669023810.1238567
GGCGAGA9200.010.00387519
GTATAAT3050.09.992841
GTCCTAA8400.09.9779691
GTCCTAC17600.09.9573541
AACAGCG1054.1489464E-59.9430717
GACGTGA9450.09.8426377
GGACCTG13450.09.8366136
CTATACT2350.09.7217424
Produced by FastQC (version 0.11.5)