##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513144_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2288305 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.678434911430077 31.0 31.0 34.0 27.0 34.0 2 30.78897349785103 33.0 31.0 34.0 27.0 34.0 3 30.885876664168457 33.0 31.0 34.0 27.0 34.0 4 34.274385189037304 37.0 35.0 37.0 30.0 37.0 5 34.010668595314 37.0 35.0 37.0 30.0 37.0 6 33.917267147517485 37.0 35.0 37.0 30.0 37.0 7 33.78165672845184 37.0 35.0 37.0 29.0 37.0 8 33.74603735079021 37.0 35.0 37.0 29.0 37.0 9 35.29656579870253 39.0 35.0 39.0 29.0 39.0 10 35.13500604158973 39.0 35.0 39.0 28.0 39.0 11 35.148595139196914 39.0 35.0 39.0 28.0 39.0 12 35.08307633816296 39.0 35.0 39.0 28.0 39.0 13 35.04997017443042 39.0 35.0 39.0 27.0 39.0 14 36.20556743965511 40.0 36.0 41.0 27.0 41.0 15 36.182546032980746 40.0 36.0 41.0 27.0 41.0 16 36.14635942324122 40.0 36.0 41.0 27.0 41.0 17 36.084136074518035 40.0 36.0 41.0 27.0 41.0 18 36.040891402151374 40.0 36.0 41.0 27.0 41.0 19 36.11587572460839 40.0 37.0 41.0 27.0 41.0 20 36.08119634401883 40.0 37.0 41.0 27.0 41.0 21 36.02475281922646 40.0 37.0 41.0 27.0 41.0 22 35.968638358960014 40.0 37.0 41.0 27.0 41.0 23 35.888296359095484 40.0 36.0 41.0 27.0 41.0 24 35.82418340212515 40.0 36.0 41.0 27.0 41.0 25 35.76886297936682 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6559328299392035 1101 2 0.5986461655538875 1101 3 0.5593142517769216 1101 4 0.741501801252646 1101 5 0.7375155477277602 1101 6 0.6904353670566081 1101 7 0.7057844233224415 1101 8 0.7407718784360497 1101 9 0.6948092461986306 1101 10 0.8849636087501409 1101 11 0.8333265467757727 1101 12 0.7695888697711766 1101 13 0.870571175450948 1101 14 1.0433758050143567 1101 15 0.9751317092286627 1101 16 0.9977117360808734 1101 17 0.9345591907496527 1101 18 0.9583508220498018 1101 19 1.0047176935810995 1101 20 1.0168855108180708 1101 21 0.9660634305786147 1101 22 1.008639231687532 1101 23 1.00903851684712 1101 24 1.0237703886638343 1101 25 1.0029444592460095 1102 1 0.5617575628390341 1102 2 0.6008874345935773 1102 3 0.5172358302518596 1102 4 0.6218131094295671 1102 5 0.7498553604628313 1102 6 0.5806222019074738 1102 7 0.6855613230598152 1102 8 0.6767628717392995 1102 9 0.6610545968313275 1102 10 0.7417648301758675 1102 11 0.7143932687515857 1102 12 0.7567967799039934 1102 13 0.7487463065905118 1102 14 0.9255524095229717 1102 15 0.830166830074063 1102 16 0.9133082910054924 1102 17 0.9712002283788337 1102 18 0.9380583541288772 1102 19 0.9345138081598279 1102 20 0.9247208652937431 1102 21 0.9340852869403946 1102 22 0.8924151075582643 1102 23 1.0074704609312377 1102 24 0.9786661498837077 1102 25 0.9217704255028067 1103 1 0.49588686269001414 1103 2 0.5120892438141205 1103 3 0.5389285084625008 1103 4 0.6167675130834027 1103 5 0.5982943445305722 1103 6 0.4614087792905863 1103 7 0.5746382612150569 1103 8 0.602909280301752 1103 9 0.7088268808820857 1103 10 0.691849618246529 1103 11 0.6310716760902864 1103 12 0.6537407340194008 1103 13 0.7093532040481634 1103 14 0.766069757982379 1103 15 0.7874700895908973 1103 16 0.8044358481323712 1103 17 0.9061454198589871 1103 18 0.8024553787363047 1103 19 0.7472547684378981 1103 20 0.8457236684217264 1103 21 0.8518423897917415 1103 22 0.8280761397232865 1103 23 0.6985123792141223 1103 24 0.815260327259054 1103 25 0.8120856642485208 1104 1 0.3325802659863122 1104 2 0.4779195472700124 1104 3 0.4218334257164038 1104 4 0.6005865855113584 1104 5 0.49913200261397606 1104 6 0.5508859719768466 1104 7 0.5196318229589423 1104 8 0.4834532961350604 1104 9 0.5571268251776615 1104 10 0.6502739973115226 1104 11 0.6418544788869127 1104 12 0.6482078926878643 1104 13 0.66170263837558 1104 14 0.7009575859057549 1104 15 0.6972493411993383 1104 16 0.789750249431826 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0.49989480564339317 1107 16 0.6249366136527357 1107 17 0.592022926284173 1107 18 0.6547874463143231 1107 19 0.6458307507246914 1107 20 0.6939858156074763 1107 21 0.63961823442488 1107 22 0.7121943125847352 1107 23 0.6792337442942582 1107 24 0.7232210266987877 1107 25 0.6985349098676892 1108 1 0.30233304065181343 1108 2 0.3799561649300678 1108 3 0.2910831055881147 1108 4 0.45244256836669194 1108 5 0.327201257854675 1108 6 0.224735082640791 1108 7 0.3077963590828148 1108 8 0.36862730001145394 1108 9 0.3878960473178097 1108 10 0.49349042262297615 1108 11 0.44187704271285355 1108 12 0.5213384544686406 1108 13 0.5673708621545686 1108 14 0.5367817941506416 1108 15 0.5658322824501454 1108 16 0.6112764759131863 1108 17 0.5909093341127871 1108 18 0.6673548583977293 1108 19 0.6655177545768538 1108 20 0.6203472509992523 1108 21 0.5525127844625857 1108 22 0.5156023361866602 1108 23 0.5862362701701187 1108 24 0.5278575663855989 1108 25 0.5756345338040774 1109 1 0.2790177393700617 1109 2 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-1.3487277713447128 2216 1 -0.5161140186915247 2216 2 -0.5099475530369943 2216 3 -0.5866572944592932 2216 4 -0.35417211506663904 2216 5 -0.5721061111861445 2216 6 -0.6415772808338147 2216 7 -0.8301900353781093 2216 8 -0.6980761912814089 2216 9 -0.8334348293609466 2216 10 -0.7856551656271691 2216 11 -0.7995470427582632 2216 12 -0.8811913115995438 2216 13 -0.825678512663707 2216 14 -1.048847270725986 2216 15 -1.0941649168241128 2216 16 -1.1266056071378543 2216 17 -1.1701607814821955 2216 18 -1.1955789025489167 2216 19 -1.135815155425881 2216 20 -1.1233757332430656 2216 21 -1.3361981179255764 2216 22 -1.2025115937613364 2216 23 -1.210694180920406 2216 24 -1.2214285719331954 2216 25 -1.2874966732207866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59303.0 3 0.0 4 1016.0 5 8138.0 6 9005.0 7 6944.0 8 5576.0 9 4907.0 10 4421.0 11 3976.0 12 3691.0 13 3439.0 14 3305.0 15 3340.0 16 3435.0 17 3733.0 18 4039.0 19 4864.0 20 6064.0 21 7993.0 22 10017.0 23 12187.0 24 14014.0 25 15518.0 26 16955.0 27 18971.0 28 21322.0 29 25805.0 30 32574.0 31 41463.0 32 53400.0 33 69177.0 34 91988.0 35 124206.0 36 177663.0 37 290384.0 38 1125472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59410863871939 19.38184661481386 13.386158050362539 27.637886696104207 2 13.629106801420377 20.619765091505055 39.840699262496585 25.910428844577982 3 16.772952382950233 24.959836935335282 30.07693477588223 28.19027590583225 4 11.23730586794694 17.42942897273127 37.577733668609625 33.755531490712166 5 12.666633137039232 38.15533110142639 36.1441657354906 13.033870026043774 6 31.881807384583276 37.47024732045033 17.942756480264038 12.70518881470235 7 27.482781652646615 31.53479775982263 22.7151921863061 18.26722840122465 8 25.1347906810863 36.771763187983176 20.652519764055118 17.44092636687541 9 26.348499753735595 14.540569384286538 19.883698519922508 39.22723234205536 10 14.378288044737076 29.038411617095257 35.011366943826054 21.571933394341613 11 34.226425236146405 22.17440419874099 24.6463211853315 18.952849379781103 12 22.79543155304909 24.786992992629916 31.980920375561823 20.43665507875917 13 28.84309565376993 21.615606311221626 25.783188866868706 23.758109168139736 14 20.96508884893627 21.651853412147016 27.669379346631906 29.713678392284816 15 23.31323840134947 30.250644035650843 23.252756953290753 23.183360609708934 16 22.338280954680428 27.775799117687544 27.414439945724016 22.471479981908008 17 22.0142920771155 28.27316233951573 27.504621169041034 22.207924414327742 18 21.936754660170283 27.33036927721298 29.086378004144898 21.646498058471842 19 23.450982277275102 26.92363998680246 27.376420538346068 22.24895719757637 20 23.71370949239721 27.081835681869332 27.529459578159383 21.674995247574078 21 23.136957907814885 26.601514582742464 26.986256531632613 23.275270977810038 22 23.355321952274718 27.49707753118575 26.724628054389605 22.422972462149932 23 22.388453680334763 27.34445573437346 27.211642466157528 23.055448119134244 24 23.084990855677017 26.977610065091845 27.610043241613337 22.327355837617798 25 22.924008348541104 26.933396961242913 27.267312385067715 22.875282305148268 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 277.0 3 404.0 4 404.0 5 404.0 6 930.5 7 1457.0 8 1457.0 9 1457.0 10 2138.0 11 2819.0 12 2819.0 13 2819.0 14 5526.0 15 8233.0 16 8233.0 17 8233.0 18 16152.0 19 24071.0 20 24071.0 21 24071.0 22 39341.5 23 54612.0 24 54612.0 25 54612.0 26 79458.5 27 104305.0 28 104305.0 29 104305.0 30 133678.5 31 163052.0 32 163052.0 33 163052.0 34 188228.5 35 213405.0 36 213405.0 37 213405.0 38 233588.5 39 253772.0 40 253772.0 41 253772.0 42 268473.0 43 283174.0 44 283174.0 45 283174.0 46 295076.0 47 306978.0 48 306978.0 49 306978.0 50 296467.0 51 285956.0 52 285956.0 53 285956.0 54 255013.0 55 224070.0 56 224070.0 57 224070.0 58 192281.0 59 160492.0 60 160492.0 61 160492.0 62 131256.5 63 102021.0 64 102021.0 65 102021.0 66 79195.0 67 56369.0 68 56369.0 69 56369.0 70 41232.0 71 26095.0 72 26095.0 73 26095.0 74 18374.0 75 10653.0 76 10653.0 77 10653.0 78 7391.5 79 4130.0 80 4130.0 81 4130.0 82 2826.5 83 1523.0 84 1523.0 85 1523.0 86 979.0 87 435.0 88 435.0 89 435.0 90 277.5 91 120.0 92 120.0 93 120.0 94 64.5 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18122584183489526 2 0.007866084285093115 3 0.005812162277318802 4 0.0018791201347722441 5 0.07734982880341562 6 0.3241700734823374 7 0.027487594529575384 8 0.013678246562411915 9 0.004981853380558973 10 0.0039767426107970745 11 0.0 12 0.0 13 0.0 14 0.0017043182617701748 15 0.0 16 0.0 17 0.02027701726824003 18 0.029454115600848662 19 0.0 20 0.0 21 7.866084285093114E-4 22 0.0 23 0.005681060872567249 24 0.0 25 4.3700468250517304E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2288305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.00128222154049 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71894570264404 53.41271579426517 2 12.995916389557355 17.41486123488552 3 3.818191393207395 7.674711573364366 4 1.4210346177107531 3.808445658712683 5 0.6622201634202709 2.218480003105812 6 0.3707870454340852 1.4905924485132183 7 0.21538485771814284 1.0101743146753743 8 0.14455197993224325 0.774813440249815 9 0.10958830321738115 0.6608301148842757 >10 0.5001551988701033 5.741751270026267 >50 0.026028961279279592 1.1949724081633013 >100 0.013930101119365374 1.9275495934567004 >500 0.0018938658159451253 0.8552482922806236 >1k 0.001371420073613693 1.8148538534169862 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 4359 0.19049034110400492 No Hit GTACATGGGAAGCAGTGGTATCAAC 4292 0.1875624097312203 No Hit GTATCAACGCAGAGTACTTTTTTTT 2940 0.12847937665652087 No Hit GTCCTACAGTGGACATTTCTAAATT 2761 0.12065699283967829 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2603 0.11375231885609653 No Hit CTTTAGGACGTGAAATATGGCGAGG 2535 0.11078068701506137 No Hit CTGTAGGACGTGGAATATGGCAAGA 2347 0.10256499898396411 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.3700468250517304E-5 0.0 3 0.0 0.0 0.0 4.3700468250517304E-5 0.0 4 0.0 0.0 0.0 4.3700468250517304E-5 0.0 5 4.3700468250517304E-5 0.0 0.0 4.3700468250517304E-5 0.0 6 4.3700468250517304E-5 0.0 0.0 4.3700468250517304E-5 0.0 7 4.3700468250517304E-5 0.0 0.0 4.3700468250517304E-5 0.0 8 8.740093650103461E-5 0.0 0.0 4.3700468250517304E-5 0.0 9 8.740093650103461E-5 0.0 0.0 8.740093650103461E-5 0.0 10 1.7480187300206922E-4 0.0 0.0 1.7480187300206922E-4 0.0 11 1.7480187300206922E-4 0.0 0.0 1.7480187300206922E-4 0.0 12 1.7480187300206922E-4 0.0 0.0 2.1850234125258652E-4 0.0 13 2.1850234125258652E-4 0.0 0.0 2.622028095031038E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 770 0.0 12.980735 4 GGCGAGG 705 0.0 12.516364 19 TGGCGAG 1495 0.0 11.808343 18 TGTACCG 130 3.1726813E-8 10.983698 5 ATGGCGA 1745 0.0 10.498484 17 TTAGGAC 1085 0.0 10.441105 3 AGGACCT 1320 0.0 10.384586 5 AGGACGT 1565 0.0 10.34035 5 TATGGCG 1715 0.0 10.294695 16 GTAGGAC 1785 0.0 10.239819 3 GGACGTG 1565 0.0 10.217579 6 GACTGCG 75 0.0026690238 10.123856 7 GGCGAGA 920 0.0 10.003875 19 GTATAAT 305 0.0 9.99284 1 GTCCTAA 840 0.0 9.977969 1 GTCCTAC 1760 0.0 9.957354 1 AACAGCG 105 4.1489464E-5 9.943071 7 GACGTGA 945 0.0 9.842637 7 GGACCTG 1345 0.0 9.836613 6 CTATACT 235 0.0 9.721742 4 >>END_MODULE