FastQCFastQC Report
Thu 26 May 2016
SRR1513143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024579
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT48660.16088189463723712No Hit
GTCCTAAAGTGTGTATTTCTCATTT41700.1378704275867815No Hit
CTGTAGGACGTGGAATATGGCAAGA39840.1317208113922632No Hit
CTTTAGGACGTGAAATATGGCGAGG35590.11766926901231543No Hit
GTCCTACAGTGTGCATTTCTCATTT30770.10173316683082173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT400.005096253514.3265311
TAGGACC10850.012.5582964
ATAGGAC4250.011.6587853
GTCCTAC25750.011.1274031
GGCGAGG11100.011.11428119
TGCACCG1201.2576857E-711.0953915
GTAGGAC27650.010.993463
TAGGACT6350.010.9540214
GACGTGG12300.010.9534487
CCAACGA1408.5019565E-910.84558419
GTCGAGA700.001506672110.84450813
AGGACCT18350.010.8319345
TGTAGGA30050.010.8207922
GGACCTG18450.010.7988176
GGACGTG24000.010.79206856
CTAGCGG803.6687634E-410.7199163
AGGACGT24800.010.5457345
CTGTAGG28900.010.5424761
TCCTACA29600.010.5020732
TCCAACG1909.094947E-1210.48864818
Produced by FastQC (version 0.11.5)