##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513143_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3024579
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.346660808000056	31.0	30.0	34.0	25.0	34.0
2	29.205788970960917	31.0	30.0	34.0	22.0	34.0
3	29.391158901784348	31.0	30.0	34.0	23.0	34.0
4	32.46046838254183	35.0	33.0	37.0	22.0	37.0
5	32.6199590091712	37.0	33.0	37.0	24.0	37.0
6	33.00146631977541	37.0	35.0	37.0	26.0	37.0
7	32.94998609723866	37.0	35.0	37.0	26.0	37.0
8	32.83206323921445	37.0	35.0	37.0	25.0	37.0
9	34.07459484443951	39.0	35.0	39.0	23.0	39.0
10	33.65364568093609	39.0	34.0	39.0	18.0	39.0
11	34.11324154535226	39.0	35.0	39.0	25.0	39.0
12	34.1090151720289	39.0	35.0	39.0	25.0	39.0
13	34.14890039241825	39.0	35.0	39.0	25.0	39.0
14	34.37069621920935	39.0	34.0	41.0	17.0	41.0
15	34.807091168721335	40.0	34.0	41.0	19.0	41.0
16	34.6547202106475	39.0	34.0	41.0	17.0	41.0
17	34.2727070445176	39.0	34.0	41.0	17.0	41.0
18	34.00880915988638	39.0	33.0	41.0	16.0	41.0
19	34.642357828973886	39.0	34.0	41.0	18.0	41.0
20	34.86194045518401	40.0	35.0	41.0	22.0	41.0
21	34.8969278038365	40.0	35.0	41.0	22.0	41.0
22	34.58282260109589	40.0	34.0	41.0	18.0	41.0
23	34.12231454361086	39.0	34.0	41.0	10.0	41.0
24	34.37765619611854	39.0	34.0	41.0	15.0	41.0
25	34.02154680039767	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9940304716537547
1101	2	1.263051028781316
1101	3	1.1861298448995683
1101	4	1.5811533141103595
1101	5	1.4287823404267996
1101	6	1.1858419209587936
1101	7	1.116613350405565
1101	8	1.1498321832019514
1101	9	1.5544895048372496
1101	10	1.8960089320207487
1101	11	1.3790086810005988
1101	12	1.3693931434570317
1101	13	1.1918037656588467
1101	14	2.261823768116699
1101	15	1.8412720699467044
1101	16	1.879692218302253
1101	17	2.2365389822848556
1101	18	2.279214867428209
1101	19	1.849516787246408
1101	20	1.4767846100676962
1101	21	1.5352279574047145
1101	22	1.8004185184203365
1101	23	1.9566568319346729
1101	24	1.6608215427853494
1101	25	1.91562057932029
1102	1	1.0509360015634712
1102	2	1.2103544683163001
1102	3	1.1548932244230947
1102	4	1.4735911406696118
1102	5	1.479731813215487
1102	6	1.100567153872504
1102	7	1.0657099550891331
1102	8	1.0982662017448703
1102	9	1.4115021104866656
1102	10	1.6777005922553556
1102	11	1.345978054906631
1102	12	1.2793192988694244
1102	13	1.2340287261617533
1102	14	2.2975434497826654
1102	15	1.8209944225240093
1102	16	1.9779038784340273
1102	17	2.296398216595918
1102	18	2.50853755231703
1102	19	1.8170794827666725
1102	20	1.670267030721007
1102	21	1.5648983887788432
1102	22	1.8022932584929663
1102	23	2.0533100649417264
1102	24	1.9099875504255124
1102	25	2.213895432826554
1103	1	0.8675104322116809
1103	2	1.1678314607171103
1103	3	0.9812954909616884
1103	4	1.3827417468129966
1103	5	1.3110105451758542
1103	6	0.9249820867076437
1103	7	0.9721196240088261
1103	8	0.963511875611232
1103	9	1.3440902120402853
1103	10	1.7435712092085751
1103	11	1.3471653733353293
1103	12	1.2369468357271103
1103	13	1.2346426538005346
1103	14	2.1488177168147544
1103	15	1.7275405361270586
1103	16	1.7347356593235475
1103	17	2.0599633737055854
1103	18	2.3450919495370286
1103	19	1.7768999750250103
1103	20	1.4625713449579578
1103	21	1.5187507112759135
1103	22	1.7019633825415994
1103	23	2.078157097086276
1103	24	1.7281985829107995
1103	25	1.9354429710942185
1104	1	0.7348539363207891
1104	2	0.9465311168341479
1104	3	0.7893614407198051
1104	4	1.083402555812242
1104	5	1.0834831511092915
1104	6	0.7058448540912181
1104	7	0.6940523628326289
1104	8	0.6646396951601048
1104	9	1.0477869538949207
1104	10	1.0758095084450332
1104	11	0.8037756490245513
1104	12	0.7322242735076117
1104	13	0.7449744425748008
1104	14	1.6747335237740089
1104	15	1.3066911743505258
1104	16	1.3997499392093076
1104	17	1.7455239320782638
1104	18	1.8771400931518727
1104	19	1.2301588489540194
1104	20	0.9575442423176383
1104	21	0.9656499360821158
1104	22	1.2628102671950927
1104	23	1.5755167753517085
1104	24	1.3543096454508685
1104	25	1.5844768931085582
1105	1	0.7705192843313391
1105	2	1.0126645877663805
1105	3	0.8931984433571181
1105	4	1.1558039432678058
1105	5	1.1795200161477055
1105	6	0.8916248678511067
1105	7	0.8316741951913045
1105	8	0.9056843001365991
1105	9	1.1319831469920416
1105	10	1.4668125470873932
1105	11	0.9915164470981637
1105	12	0.9516400515147225
1105	13	0.9003336330346059
1105	14	1.6941249353093824
1105	15	1.3259163205493536
1105	16	1.5762262607320707
1105	17	1.8379430927249771
1105	18	1.492201439327772
1105	19	1.2759778490686884
1105	20	1.1782127436362941
1105	21	1.1561728118991113
1105	22	1.3113540121784624
1105	23	1.5949182201401442
1105	24	1.4355574954680677
1105	25	1.6593335609218869
1106	1	0.34951055844604895
1106	2	0.5961266081531171
1106	3	0.49330540035983717
1106	4	0.9781723494673642
1106	5	0.9256122757343235
1106	6	0.5272076847429759
1106	7	0.4825329555060236
1106	8	0.5602673653719066
1106	9	0.7533364493953272
1106	10	0.5810101720210739
1106	11	0.47522483546215
1106	12	0.4739954761255518
1106	13	0.3563813601277488
1106	14	1.4296592123477794
1106	15	0.970461513410612
1106	16	1.0545131425544554
1106	17	1.387296270956547
1106	18	1.4954725174769408
1106	19	1.0331688025946022
1106	20	0.7557489509804896
1106	21	0.6888796424724433
1106	22	0.8650896054370705
1106	23	1.3283074307466691
1106	24	0.9061719761480802
1106	25	1.1766662395316132
1107	1	0.6846740965830378
1107	2	0.9055231037217801
1107	3	0.8374522559932842
1107	4	1.1139578584286411
1107	5	0.9417572966808336
1107	6	0.6854061058364991
1107	7	0.7223092073661093
1107	8	0.7352947063906043
1107	9	0.9617825831063556
1107	10	1.2525616179575678
1107	11	0.802843821185057
1107	12	0.7819184700716875
1107	13	0.6856053234101083
1107	14	1.6147452095628765
1107	15	1.0101627642248658
1107	16	1.210445471292111
1107	17	1.6127748881882624
1107	18	1.7756148700595418
1107	19	1.174563329012301
1107	20	1.0925469740604896
1107	21	0.9226856915607797
1107	22	1.2199917156258593
1107	23	1.4786408268298814
1107	24	1.21474338551036
1107	25	1.4822846694698768
1108	1	0.6228340522733227
1108	2	0.8223962314278594
1108	3	0.6919564070512969
1108	4	1.1931584968697067
1108	5	0.9918108226979001
1108	6	0.6315495525844383
1108	7	0.5153533562801016
1108	8	0.7055658056169705
1108	9	0.8504298563317363
1108	10	1.0068905997274555
1108	11	0.6856765609429019
1108	12	0.44803337457352654
1108	13	0.49027481700485254
1108	14	1.4127325122089545
1108	15	1.0141023981932662
1108	16	1.1214102647218809
1108	17	1.3874627399412915
1108	18	1.663123877914991
1108	19	1.0196970770543956
1108	20	0.8171284437851654
1108	21	0.8622362814636446
1108	22	1.0191053724280508
1108	23	1.367187971414154
1108	24	1.0551245592413707
1108	25	1.3355820656785369
1109	1	0.6141436785334626
1109	2	0.8272395844317124
1109	3	0.805376925861232
1109	4	1.100440686854128
1109	5	1.1443854067483912
1109	6	0.8370321974496164
1109	7	0.7887556388798913
1109	8	0.7448043926180361
1109	9	1.0527211644212358
1109	10	1.4130074139230189
1109	11	0.9705072100700178
1109	12	0.9749471521327422
1109	13	0.8821074536739744
1109	14	1.7720191485502355
1109	15	1.2327473863089935
1109	16	1.422650941715574
1109	17	1.7347626538827754
1109	18	1.8967471464995285
1109	19	1.3953553222801958
1109	20	1.2523079372693005
1109	21	1.138694153769194
1109	22	1.4030134537328607
1109	23	1.6647711613166152
1109	24	1.3998990654293877
1109	25	1.5604637352138226
1110	1	0.35713600693126324
1110	2	0.6084750424883865
1110	3	0.4590649989955722
1110	4	0.7870083665125733
1110	5	0.6199765730994926
1110	6	0.31516629525290796
1110	7	0.4168166270631062
1110	8	0.38473787776926116
1110	9	0.5106225111506077
1110	10	0.8926904104649438
1110	11	0.46384527317042057
1110	12	0.42444298295759353
1110	13	0.27987286595261196
1110	14	1.0920541473723375
1110	15	0.7541667599584088
1110	16	0.9320935708407561
1110	17	1.2044669689136143
1110	18	0.9394282954875592
1110	19	0.6344091471021258
1110	20	0.5138109779896496
1110	21	0.3155501783508967
1110	22	0.6439721822435089
1110	23	-0.5799470230926715
1110	24	0.17951600519375432
1110	25	0.6468387886554652
1111	1	0.14894450250397995
1111	2	0.5071576517842011
1111	3	0.45798802522878646
1111	4	0.856776334735379
1111	5	0.5967719640078286
1111	6	0.3299149359185094
1111	7	0.3303140780119733
1111	8	0.4964207328138741
1111	9	0.6110558055595519
1111	10	0.8914485157246901
1111	11	0.5352322133795653
1111	12	0.37818880033000113
1111	13	0.18362696292149394
1111	14	1.066478245748442
1111	15	0.6672848076805522
1111	16	0.7291686680779321
1111	17	1.0648143308624185
1111	18	1.4012163698448674
1111	19	0.8725227821706198
1111	20	0.5206828641389833
1111	21	0.29715234244328315
1111	22	0.6504552270523618
1111	23	0.9524751788935149
1111	24	0.663217724657045
1111	25	0.9741028333515089
1112	1	0.058354499225377054
1112	2	0.23233184861011225
1112	3	0.20251910091306868
1112	4	0.5583192928419791
1112	5	0.32945861785101016
1112	6	0.10854703065562887
1112	7	0.013436920483442805
1112	8	0.12756586084169186
1112	9	0.36727330345866704
1112	10	0.5523225490679806
1112	11	0.21695910901141957
1112	12	0.21538732820317108
1112	13	0.16872737436952434
1112	14	0.747145335388609
1112	15	0.5526305213812037
1112	16	0.4276859557211736
1112	17	0.8356020350897992
1112	18	1.1415617086880445
1112	19	0.4433892176565237
1112	20	0.3329087230381589
1112	21	0.3311223068486058
1112	22	0.5825522692590752
1112	23	0.9442943342221426
1112	24	0.6447315893480479
1112	25	0.9487057138234505
1113	1	0.10410359879582387
1113	2	0.1811065048483833
1113	3	0.11659668353322061
1113	4	0.3779726345494723
1113	5	0.5155094817759007
1113	6	0.22046326491582136
1113	7	0.14405887157852248
1113	8	0.27145174861365007
1113	9	0.4304970283177809
1113	10	0.6656089553875972
1113	11	0.2021337248904942
1113	12	0.1370479050902773
1113	13	0.04664565606538673
1113	14	0.715084261493864
1113	15	0.43073341239210094
1113	16	0.5410341705636128
1113	17	0.8385068996200786
1113	18	0.6859497737026246
1113	19	0.4741878649432181
1113	20	0.20097115282052158
1113	21	0.25313845279471536
1113	22	0.33737799348558895
1113	23	0.502781725941027
1113	24	0.3861140278824635
1113	25	0.7549663322034377
1114	1	-1.110658669321527
1114	2	-0.7873552015929519
1114	3	-0.7664462307348785
1114	4	-0.511072126665514
1114	5	-3.52382311178118
1114	6	-1.462038279039163
1114	7	-1.1854793124612648
1114	8	-0.7736814941150243
1114	9	-0.5057004789281052
1114	10	-0.40233343060021554
1114	11	-0.8612467616167976
1114	12	-0.9101985118960769
1114	13	-0.8910623174710679
1114	14	-6.2216644258400535
1114	15	-2.6234212818264595
1114	16	-1.539631794515273
1114	17	-0.7735217702439456
1114	18	-6.5567143539912465
1114	19	-2.6930762786959654
1114	20	-1.6838153426962634
1114	21	-1.357991046725708
1114	22	-1.1281912237948575
1114	23	-0.7427469357112955
1114	24	-0.9191821925697425
1114	25	-0.8111756989060339
1115	1	-0.7318725389583172
1115	2	-0.3307416104385794
1115	3	-0.3338642371111362
1115	4	-3.043331893948526
1115	5	-0.9995112667535473
1115	6	-0.6541033117780017
1115	7	-0.4498471586984749
1115	8	-0.23910831121947496
1115	9	-0.14360060591369006
1115	10	-0.5432295665150946
1115	11	-0.4979986731445081
1115	12	-0.5192510055992443
1115	13	-0.6918477264842124
1115	14	-5.997614961074472
1115	15	-2.370202765043416
1115	16	-1.1778417673297668
1115	17	-0.3374704857845785
1115	18	-0.21229371865035773
1115	19	-0.5018786311097756
1115	20	-0.529736459436748
1115	21	-0.5740231101698754
1115	22	-1.6311689991525995
1115	23	-0.9557431927503686
1115	24	-0.6990994454582165
1115	25	-0.2682344306553972
1116	1	-0.4270454325074695
1116	2	-0.1592257802441388
1116	3	-0.25897367414666306
1116	4	-0.17509747812172094
1116	5	-0.7836759822172574
1116	6	-0.5581431502523273
1116	7	-0.3928117995074558
1116	8	-0.3775860335717809
1116	9	-0.40778415472770035
1116	10	-0.23554182685646907
1116	11	-0.5458990090907605
1116	12	-0.6925839995929337
1116	13	-0.6765587787764531
1116	14	0.10648086844128812
1116	15	-0.31917286922068655
1116	16	-0.291829347498755
1116	17	0.131883750870891
1116	18	-0.12550166598484225
1116	19	-0.29731620958206406
1116	20	-0.74232850531547
1116	21	-0.8606879104684921
1116	22	-0.7686685749780366
1116	23	-1.4054496639573983
1116	24	-1.1523579543998181
1116	25	-0.5511054137223468
1201	1	0.7682227658224825
1201	2	0.9505397526142865
1201	3	0.9804139750489753
1201	4	1.2706448439049751
1201	5	1.174167108786186
1201	6	0.957755325947133
1201	7	0.765861597671389
1201	8	0.9369254539028589
1201	9	1.1257394571041601
1201	10	1.6192300748277333
1201	11	1.0288568525877793
1201	12	1.064183467501465
1201	13	0.9326689421274494
1201	14	1.793578099181488
1201	15	1.5789589418846575
1201	16	1.583553961460055
1201	17	1.92268433585933
1201	18	1.3180375140878056
1201	19	1.2505915132088958
1201	20	1.26121444887616
1201	21	1.2214888126527015
1201	22	1.435383885275769
1201	23	1.6267907474215662
1201	24	1.3676401585406879
1201	25	1.805100653173909
1202	1	0.8126738493189549
1202	2	0.9847111018807055
1202	3	0.9660052807330963
1202	4	1.3139931978283883
1202	5	1.1462008445033476
1202	6	0.8507351011250748
1202	7	0.8123824109404154
1202	8	0.8958633095123858
1202	9	1.1078357791435778
1202	10	1.443269254991776
1202	11	1.0168535843924573
1202	12	0.9530176743579943
1202	13	0.9645216289868017
1202	14	1.7693135624414964
1202	15	1.3612250688154859
1202	16	1.5675233870346403
1202	17	1.8391208575602818
1202	18	1.9221488159921236
1202	19	1.5064437686359184
1202	20	1.3150753386766993
1202	21	1.1858752194800246
1202	22	1.4552493394776036
1202	23	1.7184822389461232
1202	24	1.4216778210556171
1202	25	1.7815275535521806
1203	1	0.6335138336587107
1203	2	0.7929425477731833
1203	3	0.7518000583112361
1203	4	1.0255280080279547
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2211	12	-0.7565801401307652
2211	13	-0.7779129487279306
2211	14	-0.1673719715437869
2211	15	-0.4629137544971087
2211	16	-6.832339202764832
2211	17	-2.173332420373903
2211	18	-0.931121270611122
2211	19	-0.9198418475256886
2211	20	-0.982320584899675
2211	21	-0.9895515601420328
2211	22	-0.9259894103812343
2211	23	-0.48158267452218695
2211	24	-0.9710753221024575
2211	25	-7.842133925171527
2212	1	-1.4967500389165025
2212	2	-4.017486654305607
2212	3	-1.8391642419322274
2212	4	-3.6479451881621294
2212	5	-1.3187638103721433
2212	6	-1.09405733429152
2212	7	-0.9856082322083353
2212	8	-0.9063274358073485
2212	9	-0.9250381919431518
2212	10	-0.41448251943465664
2212	11	-0.8973397822123914
2212	12	-0.9986833858319812
2212	13	-1.2453202910204482
2212	14	-6.774625627497134
2212	15	-5.221180807423774
2212	16	-3.021601944509861
2212	17	-7.728697768842764
2212	18	-3.240785272390294
2212	19	-2.046352969611519
2212	20	-1.9544296312929745
2212	21	-1.675466658612514
2212	22	-1.5586087182698023
2212	23	-1.0902797331929435
2212	24	-1.4156124028840296
2212	25	-1.1961358432356946
2213	1	-0.20519163806110186
2213	2	-0.1741641001331331
2213	3	-0.16456677092108762
2213	4	0.19706000043173333
2213	5	-0.057146504122250974
2213	6	-0.2875487360233464
2213	7	-0.29875862003076037
2213	8	-0.39351171574345045
2213	9	-0.09395848330834866
2213	10	-3.9522018570181032
2213	11	-1.5111817773759029
2213	12	-1.0256283987962433
2213	13	-0.8963298528307888
2213	14	0.019261516786478694
2213	15	-0.5855205897879827
2213	16	-0.41205867517944483
2213	17	-0.02560492473083542
2213	18	0.17401231340929968
2213	19	-0.45299778652594114
2213	20	-0.7263774586972715
2213	21	-0.7398581070862775
2213	22	-1.10779760557174
2213	23	-1.2576097183412926
2213	24	-0.8193101286330844
2213	25	-0.5148455195204491
2214	1	-0.7165373273714373
2214	2	-0.46428640921447695
2214	3	-0.48327258724350486
2214	4	-0.01793304842678367
2214	5	-3.4383418110422816
2214	6	-1.2117081337082283
2214	7	-0.8617192998265608
2214	8	-0.5899519870912044
2214	9	-0.3687065005533867
2214	10	-3.7927052747057353
2214	11	-1.495909318470332
2214	12	-1.0232869029105913
2214	13	-0.7588765236733082
2214	14	0.05495282201962226
2214	15	-0.2522675485989794
2214	16	-0.31196170029554793
2214	17	-0.04551200166472569
2214	18	0.373979526761282
2214	19	-0.3539842181044861
2214	20	-0.6934068664186697
2214	21	-0.7571081011429257
2214	22	-0.4571371902810881
2214	23	-0.2584310383449733
2214	24	-0.6600823306823571
2214	25	-0.3465476861059713
2215	1	-2.2796814173205924
2215	2	-4.739834428061197
2215	3	-2.93175425987992
2215	4	-1.8823975022707415
2215	5	-1.9154838640552647
2215	6	-2.0263081875675724
2215	7	-2.1600452865561017
2215	8	-2.4249414075767923
2215	9	-8.12766126477701
2215	10	-3.8842118178063743
2215	11	-3.2093506674647188
2215	12	-3.582429607905137
2215	13	-2.8857076300779134
2215	14	-7.6552027424
2215	15	-4.6171187368454945
2215	16	-9.190397997445253
2215	17	-4.374376399624548
2215	18	-2.9596652728758777
2215	19	-3.3633900037321425
2215	20	-3.2649244243630235
2215	21	-3.3909848199343706
2215	22	-9.322628049378153
2215	23	-10.267177715162575
2215	24	-6.515912766882316
2215	25	-4.99207665170281
2216	1	-1.936939743976609
2216	2	-1.9147582590218484
2216	3	-1.8904133913417596
2216	4	-6.747487517885013
2216	5	-3.5245330557647705
2216	6	-2.7725391868661937
2216	7	-2.2684076906467965
2216	8	-8.07643184987348
2216	9	-3.9818305377896834
2216	10	-2.2401503493779593
2216	11	-2.341640283930513
2216	12	-2.4640892333077566
2216	13	-2.23007039535646
2216	14	-1.6146382560472858
2216	15	-2.252957133221379
2216	16	-1.99050963165093
2216	17	-1.6303296190858916
2216	18	-7.4988822116808365
2216	19	-4.934503154567793
2216	20	-4.187623043927715
2216	21	-3.4534624359259674
2216	22	-3.102731375014521
2216	23	-2.3034632024738997
2216	24	-2.748043938322688
2216	25	-2.157613695817286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	146091.0
3	3.0
4	1300.0
5	7034.0
6	8244.0
7	7512.0
8	6793.0
9	6585.0
10	6243.0
11	5811.0
12	5708.0
13	5560.0
14	5610.0
15	5686.0
16	5744.0
17	6318.0
18	7024.0
19	8749.0
20	11035.0
21	13961.0
22	17490.0
23	20859.0
24	23908.0
25	27814.0
26	31661.0
27	35398.0
28	39362.0
29	47167.0
30	56497.0
31	67900.0
32	87428.0
33	112918.0
34	149565.0
35	200922.0
36	288767.0
37	420573.0
38	1125339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85643790121277	18.92570109878027	12.521133892752925	25.69672710725403
2	13.90528434949829	20.253021046633165	42.377693731359315	23.46400087250923
3	18.218249708779833	24.97638083443114	29.917311373200057	26.888058083588973
4	10.964165551234062	17.026594348991463	39.9026501350144	32.106589964760076
5	12.236434899983823	38.41022941533696	36.80785570515183	12.54547997952738
6	31.302471266876452	37.959369531315104	18.54776199754236	12.190397204266088
7	27.771485562756055	31.58766528914005	22.78636476821078	17.854484379893115
8	24.52679385142848	37.575279790162334	21.006583220129812	16.891343138279368
9	26.313264087988546	14.993700459320841	20.423276224111532	38.26975922857908
10	13.346296489200402	29.331521137053958	36.36109002944819	20.96109234429745
11	34.13062564794468	22.422796409373724	25.095197231467747	18.351380711213846
12	22.820924941132894	24.855081091091886	32.58340609043046	19.740587877344762
13	28.758455470665545	22.02648868205446	25.969332445262257	23.245723402017738
14	19.867268899531656	22.026596487004348	29.20014762597979	28.9059869874842
15	22.600890868744212	31.333481734280056	23.483490615183666	22.582136781792066
16	21.592500170306373	28.25055341303892	28.62588881738593	21.531057599268774
17	21.093722350575273	28.779150245836977	28.701785605016962	21.425341798570788
18	20.910806488099727	27.795581996197942	30.4936321531617	20.79997936254063
19	23.04737274640889	27.300485980319365	27.85224478592228	21.799896487349464
20	23.28281777656366	27.689686340096486	28.22859758394464	20.79889829939521
21	22.7063204452269	27.18504850445025	27.268361524929247	22.8402695253936
22	22.685180284835752	28.423076617747455	27.501541404914505	21.390201692502288
23	21.75626027504631	28.030309795023268	28.177215466170853	22.036214463759574
24	22.662471866701424	27.383573461484005	28.405832672865145	21.548121998949426
25	22.392369279862198	27.570173671713526	28.14411818500838	21.89333886341589
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	172.0
2	330.5
3	489.0
4	489.0
5	489.0
6	1043.5
7	1598.0
8	1598.0
9	1598.0
10	2811.0
11	4024.0
12	4024.0
13	4024.0
14	8353.0
15	12682.0
16	12682.0
17	12682.0
18	24037.5
19	35393.0
20	35393.0
21	35393.0
22	58055.0
23	80717.0
24	80717.0
25	80717.0
26	115746.5
27	150776.0
28	150776.0
29	150776.0
30	194786.5
31	238797.0
32	238797.0
33	238797.0
34	275211.5
35	311626.0
36	311626.0
37	311626.0
38	338002.5
39	364379.0
40	364379.0
41	364379.0
42	379706.5
43	395034.0
44	395034.0
45	395034.0
46	400565.0
47	406096.0
48	406096.0
49	406096.0
50	383545.0
51	360994.0
52	360994.0
53	360994.0
54	315555.0
55	270116.0
56	270116.0
57	270116.0
58	227208.5
59	184301.0
60	184301.0
61	184301.0
62	147425.5
63	110550.0
64	110550.0
65	110550.0
66	83782.5
67	57015.0
68	57015.0
69	57015.0
70	41105.0
71	25195.0
72	25195.0
73	25195.0
74	17351.0
75	9507.0
76	9507.0
77	9507.0
78	6493.0
79	3479.0
80	3479.0
81	3479.0
82	2292.0
83	1105.0
84	1105.0
85	1105.0
86	740.5
87	376.0
88	376.0
89	376.0
90	245.5
91	115.0
92	115.0
93	115.0
94	72.0
95	29.0
96	29.0
97	29.0
98	21.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16994100666572107
2	0.2642351216483352
3	0.26321018561591547
4	0.4558981597108226
5	0.2601023150659976
6	0.017952911793674423
7	0.018514973488872336
8	0.017423912551135214
9	0.018151286509626628
10	0.020630970459029174
11	0.019639096879268157
12	0.02707814872747579
13	0.026350774768984377
14	0.02383802836692313
15	0.040931316391471344
16	0.02039953329041827
17	0.04079906658083654
18	0.03144239247842427
19	0.026251587411008276
20	0.029425582866243534
21	0.034451075670366024
22	0.04506412297380892
23	0.028764333813069524
24	0.04863486786094858
25	0.03689769716710987
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3024579.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.96212819358658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1357256840416	62.228573287035196
2	11.04203765247785	16.33385208741953
3	2.666640830686271	5.9169129289641065
4	0.9296693588664473	2.750412971925185
5	0.4177874971525452	1.5450226211037117
6	0.21532160119770116	0.955538632237962
7	0.12608227649861328	0.6527719448130764
8	0.08146118522788266	0.4820034099700927
9	0.06314637755945217	0.42033964248115385
>10	0.29233200326550807	3.8213089705210423
>50	0.016067943670665864	0.8346836055400932
>100	0.011059035040497607	1.6163193826288142
>500	0.0017639596323470754	0.9372157167790042
>1k	9.045946826434352E-4	1.505044798581082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4866	0.16088189463723712	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4170	0.1378704275867815	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3984	0.1317208113922632	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3559	0.11766926901231543	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3077	0.10173316683082173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.91873579761018E-5	0.0	0.0	0.0	0.0
7	9.91873579761018E-5	0.0	0.0	0.0	0.0
8	1.322498106348024E-4	0.0	0.0	0.0	0.0
9	1.322498106348024E-4	0.0	0.0	0.0	0.0
10	1.322498106348024E-4	0.0	0.0	6.61249053174012E-5	0.0
11	1.322498106348024E-4	0.0	0.0	6.61249053174012E-5	0.0
12	1.322498106348024E-4	0.0	0.0	6.61249053174012E-5	0.0
13	1.322498106348024E-4	0.0	0.0	6.61249053174012E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	40	0.0050962535	14.326531	1
TAGGACC	1085	0.0	12.558296	4
ATAGGAC	425	0.0	11.658785	3
GTCCTAC	2575	0.0	11.127403	1
GGCGAGG	1110	0.0	11.114281	19
TGCACCG	120	1.2576857E-7	11.095391	5
GTAGGAC	2765	0.0	10.99346	3
TAGGACT	635	0.0	10.954021	4
GACGTGG	1230	0.0	10.953448	7
CCAACGA	140	8.5019565E-9	10.845584	19
GTCGAGA	70	0.0015066721	10.844508	13
AGGACCT	1835	0.0	10.831934	5
TGTAGGA	3005	0.0	10.820792	2
GGACCTG	1845	0.0	10.798817	6
GGACGTG	2400	0.0	10.7920685	6
CTAGCGG	80	3.6687634E-4	10.719916	3
AGGACGT	2480	0.0	10.545734	5
CTGTAGG	2890	0.0	10.542476	1
TCCTACA	2960	0.0	10.502073	2
TCCAACG	190	9.094947E-12	10.488648	18
>>END_MODULE