FastQCFastQC Report
Thu 26 May 2016
SRR1513143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024579
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51250.16944506987584057No Hit
CTTTAGGACGTGAAATATGGCGAGG42860.14170567209519078No Hit
CTGTAGGACGTGGAATATGGCAAGA42770.14140811002126247No Hit
GTCCTAAAGTGTGTATTTCTCATTT42660.14104442304201675No Hit
GTACATGGGAAGCAGTGGTATCAAC32270.10669253472962684No Hit
GTCCTACAGTGTGCATTTCTCATTT30840.10196460399943266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG905.2786345E-712.6924875
TAGGACC10700.012.54420954
ACCGCTC600.005925144511.0714028
GGCCCGT951.3328387E-511.0209616
ATAGGAC3550.010.9951083
GGACCTG18650.010.9215766
AGGACCT20150.010.4878375
TCCAACG1553.7271093E-910.40917118
TCGTCAA1951.6370905E-1110.21857310
GTCCTAC28450.010.1444331
TGGCGAG27800.010.10522118
GGCGAGG11550.010.02184119
GCCGTGC951.6629648E-49.9892358
GTAGGAC27300.09.8340063
GGACGTG24950.09.8042176
GTCCTAA14800.09.7824871
AGGACGT25250.09.7644195
ATTTAGA9950.09.7643771
AAGTCGT1752.6157068E-99.7630087
TCCTACA32500.09.7557932
Produced by FastQC (version 0.11.5)