##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513143_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3024579 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.651123677047284 31.0 31.0 34.0 27.0 34.0 2 30.76906306629782 33.0 31.0 34.0 27.0 34.0 3 30.864857555382088 33.0 31.0 34.0 27.0 34.0 4 34.24033526649494 37.0 35.0 37.0 30.0 37.0 5 33.97698390420617 37.0 35.0 37.0 30.0 37.0 6 33.89245776023704 37.0 35.0 37.0 30.0 37.0 7 33.756897075593 37.0 35.0 37.0 29.0 37.0 8 33.72171796471509 37.0 35.0 37.0 28.0 37.0 9 35.27450696444034 39.0 35.0 39.0 29.0 39.0 10 35.11000737623319 39.0 35.0 39.0 28.0 39.0 11 35.12416934720502 39.0 35.0 39.0 28.0 39.0 12 35.05784441404903 39.0 35.0 39.0 27.0 39.0 13 35.02772286655432 39.0 35.0 39.0 27.0 39.0 14 36.17428475169602 40.0 36.0 41.0 27.0 41.0 15 36.16681230677063 40.0 36.0 41.0 27.0 41.0 16 36.12959324256368 40.0 36.0 41.0 27.0 41.0 17 36.05841308823476 40.0 36.0 41.0 27.0 41.0 18 36.012301546760725 40.0 36.0 41.0 27.0 41.0 19 36.094596967048965 40.0 37.0 41.0 27.0 41.0 20 36.06034691109077 40.0 37.0 41.0 27.0 41.0 21 36.00875063934518 40.0 37.0 41.0 27.0 41.0 22 35.946842849864396 40.0 37.0 41.0 27.0 41.0 23 35.869626814178105 40.0 37.0 41.0 27.0 41.0 24 35.81054090503174 40.0 36.0 41.0 27.0 41.0 25 35.75573757537826 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5101217582400075 1101 2 0.5748278007854246 1101 3 0.464390351903468 1101 4 0.6601080463423727 1101 5 0.6215402356693787 1101 6 0.5918277198669912 1101 7 0.6632906903432598 1101 8 0.6766070120255705 1101 9 0.6968940325222803 1101 10 0.7339563464965266 1101 11 0.7295129313858979 1101 12 0.8115007028590426 1101 13 0.7984426272078338 1101 14 0.9606120441615502 1101 15 0.9555441207329309 1101 16 0.9020125925669404 1101 17 0.9450420208311527 1101 18 0.9321558294036407 1101 19 0.9159221663739885 1101 20 0.8983320310766985 1101 21 1.0031179065795826 1101 22 0.9446165312911603 1101 23 1.0084678897512802 1101 24 1.01700626956724 1101 25 1.0599477052930482 1102 1 0.5537107503516623 1102 2 0.5840246566011054 1102 3 0.5529198595805092 1102 4 0.6407949111579896 1102 5 0.6734849719707725 1102 6 0.6593106005039928 1102 7 0.6291353685237553 1102 8 0.6816225844313735 1102 9 0.6603668579869435 1102 10 0.81110175216714 1102 11 0.8509072455840325 1102 12 0.8914629648160002 1102 13 0.795966951861395 1102 14 0.9349794726490188 1102 15 0.8643612526930724 1102 16 0.880133060832712 1102 17 0.9412861630100195 1102 18 0.9388700389248328 1102 19 0.9569463934342792 1102 20 1.0005693395511415 1102 21 0.9418645815362368 1102 22 1.0068595826402458 1102 23 1.0600082121868013 1102 24 1.0093706094797597 1102 25 0.9935145208908054 1103 1 0.5229371134032057 1103 2 0.5754700596343163 1103 3 0.5941019199686615 1103 4 0.6578957333510331 1103 5 0.6427145662204126 1103 6 0.4507043043676262 1103 7 0.5143372155940895 1103 8 0.46630332737193925 1103 9 0.6753643170902563 1103 10 0.6397849508182603 1103 11 0.7173616812846575 1103 12 0.6535323709511616 1103 13 0.7628890300505162 1103 14 0.7769044148546342 1103 15 0.7654962844616264 1103 16 0.7657320515239263 1103 17 0.8602904546285828 1103 18 0.7749335877790742 1103 19 0.7500311888847548 1103 20 0.7938875156279366 1103 21 0.7688428825722795 1103 22 0.8220935066953459 1103 23 0.7400937508128322 1103 24 0.8400827067916978 1103 25 0.8026254714211589 1104 1 0.29532739142383946 1104 2 0.3420281700265768 1104 3 0.3323610853239103 1104 4 0.4476280583495793 1104 5 0.4449133772013596 1104 6 0.37826448388619127 1104 7 0.36405963210047076 1104 8 0.45545279989044474 1104 9 0.3498725353102259 1104 10 0.5100011890883565 1104 11 0.5745548094539217 1104 12 0.5162494834999762 1104 13 0.4964904755246664 1104 14 0.6217855129487049 1104 15 0.48662031546938067 1104 16 0.532782672261817 1104 17 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-0.7086297514334099 2216 4 -0.5078038532137157 2216 5 -0.8669189452404353 2216 6 -0.8762812671083964 2216 7 -1.1664880428489823 2216 8 -1.1540324606586978 2216 9 -1.262214955722186 2216 10 -1.3083839931982197 2216 11 -1.1435605300099851 2216 12 -1.3130492512551228 2216 13 -1.351980143401832 2216 14 -1.6125081673486932 2216 15 -1.5233793584365785 2216 16 -1.4560575742652802 2216 17 -1.59204432456891 2216 18 -1.521648751617441 2216 19 -1.5832088013822627 2216 20 -1.6637305071241144 2216 21 -1.777058136942884 2216 22 -1.6827759549331347 2216 23 -1.7580335593359706 2216 24 -1.6206305876400222 2216 25 -1.8825218656688634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80727.0 3 0.0 4 1311.0 5 10964.0 6 12034.0 7 9236.0 8 7328.0 9 6302.0 10 5950.0 11 5248.0 12 4917.0 13 4548.0 14 4439.0 15 4537.0 16 4422.0 17 4767.0 18 5259.0 19 6209.0 20 8085.0 21 10520.0 22 12966.0 23 15989.0 24 18373.0 25 20312.0 26 22868.0 27 24873.0 28 27956.0 29 33876.0 30 42995.0 31 54668.0 32 69671.0 33 90988.0 34 121135.0 35 163317.0 36 236066.0 37 385219.0 38 1486504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.071567307743265 19.71926621549289 13.62573730875019 27.58342916801365 2 13.283587567548444 20.576852049937987 40.728384084837835 25.411176297675738 3 16.58992970839515 24.993048210207576 30.20852724225392 28.208494839143356 4 10.771851423742286 17.629791958588775 38.414789382951334 33.18356723471761 5 12.159624584028343 38.6213614785015 36.61606126252878 12.602952674941376 6 31.320043348301347 38.44597600186555 18.07250656875373 12.161474081079367 7 27.457101998753835 31.715781146442552 22.81931577506115 18.00780107974246 8 24.45121080171634 38.01370451888025 20.554893469554038 16.980191209849373 9 25.99089345153132 14.846596881316302 20.12083651163183 39.04167315552055 10 13.698353850004514 29.71130958974042 35.663298892923045 20.927037667332023 11 34.01706485431526 22.392471811779423 25.009166564999624 18.581296768905688 12 22.515008705346663 25.01377051608522 32.57842912300492 19.892791655563187 13 28.624810262849802 21.96818135681032 26.007057511144527 23.399950869195347 14 20.1911110640881 22.172447631428945 28.142444893808083 29.49399641067487 15 22.515166573595863 31.44394641369923 23.346654195509522 22.694232817195385 16 21.711484474368167 28.54450156534182 27.938070058675933 21.805943901614075 17 21.26645956283322 28.95818217773848 28.19606035332875 21.579297906099555 18 21.1181710064518 28.100952093693323 29.80647243796574 20.974404461889133 19 22.974139541403943 27.4215022983364 27.65393134052706 21.950426819732595 20 23.14675860673502 27.776196290458937 28.149107693996424 20.927937408809623 21 22.47196026380149 27.199147508701305 27.34869056423987 22.98020166325734 22 22.571700156220295 28.4468727011233 27.364869444467406 21.616557698189002 23 21.70039902182122 28.169492354837132 27.781315674270168 22.348792949071484 24 22.53731841687719 27.42444485662302 28.39896064873822 21.639276077761565 25 22.317871992636324 27.679020133731143 27.941470143253138 22.0616377303794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 95.0 2 234.0 3 373.0 4 373.0 5 373.0 6 986.0 7 1599.0 8 1599.0 9 1599.0 10 2799.0 11 3999.0 12 3999.0 13 3999.0 14 7987.0 15 11975.0 16 11975.0 17 11975.0 18 23152.5 19 34330.0 20 34330.0 21 34330.0 22 56331.0 23 78332.0 24 78332.0 25 78332.0 26 113761.5 27 149191.0 28 149191.0 29 149191.0 30 193165.5 31 237140.0 32 237140.0 33 237140.0 34 272551.0 35 307962.0 36 307962.0 37 307962.0 38 333123.5 39 358285.0 40 358285.0 41 358285.0 42 375344.5 43 392404.0 44 392404.0 45 392404.0 46 401483.0 47 410562.0 48 410562.0 49 410562.0 50 388661.5 51 366761.0 52 366761.0 53 366761.0 54 319828.0 55 272895.0 56 272895.0 57 272895.0 58 230186.0 59 187477.0 60 187477.0 61 187477.0 62 150177.5 63 112878.0 64 112878.0 65 112878.0 66 85705.5 67 58533.0 68 58533.0 69 58533.0 70 41950.5 71 25368.0 72 25368.0 73 25368.0 74 17488.5 75 9609.0 76 9609.0 77 9609.0 78 6453.5 79 3298.0 80 3298.0 81 3298.0 82 2210.0 83 1122.0 84 1122.0 85 1122.0 86 713.5 87 305.0 88 305.0 89 305.0 90 189.0 91 73.0 92 73.0 93 73.0 94 42.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18154592754892498 2 0.00886073731253176 3 0.005885116573248707 4 0.002810308475989551 5 0.08060625958191206 6 0.32824402999557956 7 0.02959089512953704 8 0.01544016539161318 9 0.005356117330709497 10 0.004231993940313677 11 0.0 12 3.30624526587006E-5 13 0.0 14 0.0018184348962285329 15 0.0 16 0.0 17 0.021589781586131493 18 0.030648893614615456 19 0.0 20 0.0 21 0.0010579984850784192 22 1.322498106348024E-4 23 0.00605042883654221 24 0.0 25 9.91873579761018E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3024579.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.29072723498993 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4012878328733 58.74447734705265 2 12.011239280605865 17.12579966615739 3 3.0670805883388237 6.559632168929866 4 1.1053330722409909 3.151999942277836 5 0.48743520590428185 1.7374805154426656 6 0.2514505795003917 1.0755656805745544 7 0.14497976018843445 0.7234998776731554 8 0.10113050909301147 0.576773403110844 9 0.07083861631175303 0.45451228258667653 >10 0.32256390877949837 4.108041294575047 >50 0.02055894583751313 1.0297535904990125 >100 0.01308362045023325 1.862539484583646 >500 0.0018572799242254163 0.9975531057673372 >1k 0.0011143679536066316 1.6827253816745422 >5k 4.6431998066942986E-5 0.16964625909475814 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5125 0.16944506987584057 No Hit CTTTAGGACGTGAAATATGGCGAGG 4286 0.14170567209519078 No Hit CTGTAGGACGTGGAATATGGCAAGA 4277 0.14140811002126247 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4266 0.14104442304201675 No Hit GTACATGGGAAGCAGTGGTATCAAC 3227 0.10669253472962684 No Hit GTCCTACAGTGTGCATTTCTCATTT 3084 0.10196460399943266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.30624526587006E-5 0.0 5 0.0 0.0 0.0 3.30624526587006E-5 0.0 6 3.30624526587006E-5 0.0 0.0 3.30624526587006E-5 0.0 7 3.30624526587006E-5 0.0 0.0 3.30624526587006E-5 0.0 8 6.61249053174012E-5 0.0 0.0 3.30624526587006E-5 0.0 9 9.91873579761018E-5 0.0 0.0 3.30624526587006E-5 0.0 10 9.91873579761018E-5 0.0 0.0 3.30624526587006E-5 0.0 11 1.322498106348024E-4 0.0 0.0 3.30624526587006E-5 0.0 12 1.322498106348024E-4 0.0 0.0 3.30624526587006E-5 0.0 13 1.322498106348024E-4 0.0 0.0 3.30624526587006E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 90 5.2786345E-7 12.692487 5 TAGGACC 1070 0.0 12.5442095 4 ACCGCTC 60 0.0059251445 11.071402 8 GGCCCGT 95 1.3328387E-5 11.020961 6 ATAGGAC 355 0.0 10.995108 3 GGACCTG 1865 0.0 10.921576 6 AGGACCT 2015 0.0 10.487837 5 TCCAACG 155 3.7271093E-9 10.409171 18 TCGTCAA 195 1.6370905E-11 10.218573 10 GTCCTAC 2845 0.0 10.144433 1 TGGCGAG 2780 0.0 10.105221 18 GGCGAGG 1155 0.0 10.021841 19 GCCGTGC 95 1.6629648E-4 9.989235 8 GTAGGAC 2730 0.0 9.834006 3 GGACGTG 2495 0.0 9.804217 6 GTCCTAA 1480 0.0 9.782487 1 AGGACGT 2525 0.0 9.764419 5 ATTTAGA 995 0.0 9.764377 1 AAGTCGT 175 2.6157068E-9 9.763008 7 TCCTACA 3250 0.0 9.755793 2 >>END_MODULE