FastQCFastQC Report
Thu 26 May 2016
SRR1513142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232539
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27240.12201354601196217No Hit
GTCCTAAAGTGTGTATTTCTCATTT27060.12120728909998885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG1001.4621764E-712.33782419
TAAGACG657.838976E-411.7251824
GGCGAGG6600.011.64759619
TGGCGAG15350.011.43822318
TAGGACC6750.011.29091554
CGGGCAA600.0059262511.07091710
TAGGACT5350.010.862234
CCGAGCA700.001505857310.844989
GTCCTAA9600.010.542791
AGGACCT12300.010.5153865
GGCGAGA9850.010.50232519
GTCTTAA2900.010.2066831
GTCCTAC15250.010.2056051
TATGGCG16650.010.20337316
GCGCTAC750.002539463310.1847331
AGGACGT14500.010.1661185
TTAGGAC11250.010.162513
GTCTATA2750.010.0689981
ATGGCGA16700.010.0591617
GACGTGA8500.010.0455297
Produced by FastQC (version 0.11.5)