##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513142_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2232539
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.78638088741115	31.0	30.0	34.0	20.0	34.0
2	28.63835167045234	31.0	29.0	34.0	16.0	34.0
3	28.808680609834813	31.0	30.0	34.0	16.0	34.0
4	31.88435543567212	35.0	32.0	37.0	17.0	37.0
5	32.02290351926663	35.0	32.0	37.0	19.0	37.0
6	32.403488584074005	36.0	33.0	37.0	23.0	37.0
7	32.3319552312412	36.0	33.0	37.0	22.0	37.0
8	32.21793303498841	36.0	33.0	37.0	19.0	37.0
9	33.36360081503615	38.0	33.0	39.0	17.0	39.0
10	32.93372971312035	38.0	33.0	39.0	17.0	39.0
11	33.38114182999715	38.0	33.0	39.0	18.0	39.0
12	33.366095284337696	38.0	33.0	39.0	17.0	39.0
13	33.407717849497814	38.0	34.0	39.0	18.0	39.0
14	33.55987241432288	39.0	32.0	41.0	10.0	41.0
15	33.971953905396504	39.0	33.0	41.0	17.0	41.0
16	33.794742667429325	39.0	33.0	41.0	11.0	41.0
17	33.41723302482062	38.0	32.0	41.0	10.0	41.0
18	33.13405320131026	38.0	32.0	41.0	10.0	41.0
19	33.761438433998244	39.0	33.0	41.0	10.0	41.0
20	33.97696165666087	39.0	34.0	41.0	10.0	41.0
21	34.009533987984085	39.0	34.0	41.0	10.0	41.0
22	33.68485836081699	39.0	33.0	41.0	2.0	41.0
23	33.19599702401615	39.0	32.0	41.0	2.0	41.0
24	33.42509402971236	39.0	33.0	41.0	2.0	41.0
25	33.03486926768132	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0361844638734787
1101	2	1.2929783806234205
1101	3	1.1684805244292171
1101	4	1.5900656976231886
1101	5	1.4253461954733098
1101	6	1.160460546264595
1101	7	1.1042316586940828
1101	8	1.236169270755731
1101	9	1.5547326837222855
1101	10	1.9254167538805973
1101	11	1.5609451729656385
1101	12	1.4481466166556913
1101	13	1.3653775512914663
1101	14	2.19562737085532
1101	15	1.9953760929597237
1101	16	2.044441710874217
1101	17	2.392028548191682
1101	18	2.4453048417606027
1101	19	2.022691995524454
1101	20	1.571824957939583
1101	21	1.6387460721666827
1101	22	1.8572026622843367
1101	23	2.150648684487649
1101	24	1.7474008197871242
1101	25	2.156140083742578
1102	1	1.2145272216858984
1102	2	1.4059816876671754
1102	3	1.4015551771163146
1102	4	1.749608154512039
1102	5	1.7423500266592171
1102	6	1.4915060724089315
1102	7	1.4013209287161743
1102	8	1.4935897766262158
1102	9	1.8223615910783266
1102	10	2.0023318657602047
1102	11	1.6729134825247272
1102	12	1.5735859293330563
1102	13	1.5185070556380893
1102	14	2.5146024163741316
1102	15	2.1773290172972324
1102	16	2.1942081835533074
1102	17	2.6156277737075087
1102	18	2.90185858878138
1102	19	2.2630409532421254
1102	20	2.2311894630696756
1102	21	2.03132249658254
1102	22	2.1571089214762935
1102	23	2.504520509739386
1102	24	2.3034550739978954
1102	25	2.588248647144077
1103	1	0.7295936535847503
1103	2	1.0027139612997047
1103	3	0.761432419874712
1103	4	1.212695423406842
1103	5	1.0374772421452363
1103	6	0.8148708794151815
1103	7	0.7988288196735525
1103	8	0.771929921291516
1103	9	1.1681429378558121
1103	10	1.4615553829318841
1103	11	1.0233608847070528
1103	12	0.983257209313507
1103	13	0.9090583723303141
1103	14	1.8105666458371275
1103	15	1.4660109366593588
1103	16	1.3730522135177594
1103	17	1.810941974004848
1103	18	2.0375371660379287
1103	19	1.40276161199845
1103	20	1.0498409726068871
1103	21	1.1604987085804055
1103	22	1.3626142447733969
1103	23	1.676623710208922
1103	24	1.4486183070500331
1103	25	1.7117052335803749
1104	1	0.7125916052487717
1104	2	0.9851687861896217
1104	3	0.8496711992504657
1104	4	1.169558678390061
1104	5	1.091581549606751
1104	6	0.9106889716522559
1104	7	0.8677185304612038
1104	8	0.9121495136149562
1104	9	1.2071193743255364
1104	10	1.3390063776613061
1104	11	0.9667402730282504
1104	12	0.7872818602495286
1104	13	0.8504956924605622
1104	14	1.9001467569033679
1104	15	1.5517831334431946
1104	16	1.5749731522336816
1104	17	1.9014969302715272
1104	18	1.8664399125771993
1104	19	1.3855544730366134
1104	20	1.1063056835031162
1104	21	1.2098309808312067
1104	22	1.4858444629006584
1104	23	1.7059730296209068
1104	24	1.393599688915927
1104	25	1.7321277043161842
1105	1	0.7124572813077314
1105	2	0.9113540615204485
1105	3	0.772652564188224
1105	4	1.1312763551931795
1105	5	1.2153697682682747
1105	6	0.8569789969051556
1105	7	0.7418149941275018
1105	8	0.817398630708503
1105	9	0.989133057340986
1105	10	1.3346261045715408
1105	11	0.8201053458983338
1105	12	0.906439534504436
1105	13	0.754985682395457
1105	14	1.4666784987444785
1105	15	1.1415078507459882
1105	16	1.4312673620930099
1105	17	1.5652075368224416
1105	18	1.1644406822927635
1105	19	1.0580675418372465
1105	20	0.9872676319370726
1105	21	1.0008672948492503
1105	22	0.9913965482995337
1105	23	1.4037565883355256
1105	24	1.2054566513327032
1105	25	1.4574658724946588
1106	1	0.8104350194187191
1106	2	0.8782989874830882
1106	3	0.881273849220463
1106	4	1.4531824456872968
1106	5	1.2555232890586936
1106	6	0.9368268161574491
1106	7	0.9025857592059836
1106	8	1.0438218677362912
1106	9	1.1033371664616922
1106	10	1.0269493778491992
1106	11	0.8592813227121567
1106	12	0.8934964399335001
1106	13	0.7838167012917765
1106	14	1.7856337464719871
1106	15	1.4334818539174279
1106	16	1.532100250177109
1106	17	1.7770495629670222
1106	18	1.7173536430827667
1106	19	1.4338115661916646
1106	20	1.1500271578368313
1106	21	1.0131779923414825
1106	22	1.263969481842004
1106	23	1.761749685397561
1106	24	1.3660095735707074
1106	25	1.591465333041569
1107	1	0.6313497814556044
1107	2	0.8578645961142435
1107	3	0.7790885674876193
1107	4	1.1249822586328477
1107	5	0.9716675723019748
1107	6	0.7204549977908599
1107	7	0.7168009696506772
1107	8	0.7970357381019895
1107	9	0.9205071287893674
1107	10	1.1992255977301056
1107	11	0.7873179003747524
1107	12	0.7912555816082687
1107	13	0.7301977711791281
1107	14	1.548405104033563
1107	15	1.0637161105193584
1107	16	1.2634916637200888
1107	17	1.6451415148186328
1107	18	1.7793842886398892
1107	19	1.305725484037822
1107	20	1.0536442747526138
1107	21	0.9692343650538007
1107	22	1.236441105311016
1107	23	1.5111773476976609
1107	24	1.243703372690426
1107	25	1.5658927634211324
1108	1	0.7886931919886564
1108	2	0.9222173615360703
1108	3	0.8046630159444099
1108	4	1.3311963506660263
1108	5	1.2670360548484894
1108	6	0.8402209118224917
1108	7	0.7717274025551859
1108	8	0.8440827327822191
1108	9	1.0311151025765852
1108	10	1.3708233243718695
1108	11	0.9469613170790794
1108	12	0.6737613956652382
1108	13	0.6476666066527557
1108	14	1.5987578065782415
1108	15	1.1873479711356936
1108	16	1.272171870709002
1108	17	1.6083671158651214
1108	18	1.7843793624315438
1108	19	1.2361980789993652
1108	20	0.9851453602151281
1108	21	1.0196117693353983
1108	22	1.3034989188529877
1108	23	1.6914136959126367
1108	24	1.341634115673756
1108	25	1.7182792755741403
1109	1	0.6064157471349318
1109	2	0.6914548490648329
1109	3	0.6590995900742058
1109	4	0.9212105773287789
1109	5	1.0372320301409559
1109	6	0.7735590825843843
1109	7	0.7431587547386371
1109	8	0.6840702545279456
1109	9	0.8525178181735953
1109	10	1.2460412769442684
1109	11	0.8063659963749075
1109	12	0.8605887475099081
1109	13	0.6981081912358533
1109	14	1.7360142284297524
1109	15	1.2583045453909634
1109	16	1.2671548134075934
1109	17	1.6837995497834513
1109	18	1.8680147048496707
1109	19	1.2126308039889082
1109	20	1.2389631727497488
1109	21	1.0497004098479223
1109	22	1.334494288294671
1109	23	1.707059557839223
1109	24	1.43180997518823
1109	25	1.668937586862505
1110	1	0.2836854972381886
1110	2	0.5177124829124224
1110	3	0.4488659738183749
1110	4	0.765990685269827
1110	5	0.7255065193526207
1110	6	0.40267218005337213
1110	7	0.3912436136877844
1110	8	0.3844420456089779
1110	9	0.5616934005413512
1110	10	0.9483379496794413
1110	11	0.41423106380262453
1110	12	0.30914130375076354
1110	13	0.17310450760669482
1110	14	0.9612368366501585
1110	15	0.719486345922526
1110	16	0.7886560002448917
1110	17	1.039962236647284
1110	18	0.7839092927331492
1110	19	0.4474202998278116
1110	20	0.2931410345175678
1110	21	0.18343452533027005
1110	22	0.48660691142430323
1110	23	-0.8864871523915099
1110	24	0.03661087297188459
1110	25	0.588362937417088
1111	1	0.15674742831084743
1111	2	0.5471583169291527
1111	3	0.4602028761883936
1111	4	0.8200954832237386
1111	5	0.6990011042919306
1111	6	0.4225080752682331
1111	7	0.3387760835500089
1111	8	0.3709082379986768
1111	9	0.682467769595803
1111	10	0.884981675589728
1111	11	0.4715421645488931
1111	12	0.34983754780521537
1111	13	0.23203782009644414
1111	14	1.172183946909307
1111	15	0.673621704500512
1111	16	0.8505598438360025
1111	17	1.2939979048037529
1111	18	1.5523481766104652
1111	19	0.9522142174307291
1111	20	0.47649741111671773
1111	21	0.25753955393040684
1111	22	0.7491143433580376
1111	23	1.0241380782547083
1111	24	0.758751692739807
1111	25	0.8930084726188312
1112	1	0.04173356235014225
1112	2	0.2558020196187982
1112	3	0.19616138903264968
1112	4	0.45641442296846435
1112	5	0.18848367243897712
1112	6	-0.004075626314147485
1112	7	-0.13922648832146933
1112	8	-0.008941073484649564
1112	9	0.22453089117335168
1112	10	0.43321842114053766
1112	11	0.009331940478993772
1112	12	-0.005755140228281164
1112	13	-0.053677094744919884
1112	14	0.5175564656321257
1112	15	0.33277764245348607
1112	16	0.24747013786483052
1112	17	0.6174528945363988
1112	18	0.7979719686864826
1112	19	0.30604377684046113
1112	20	0.30213075334171435
1112	21	0.22766765678512968
1112	22	0.474411039685954
1112	23	0.8537371229881572
1112	24	0.4950036933638984
1112	25	0.9555962358455972
1113	1	-0.14044911408089789
1113	2	0.005062505516768567
1113	3	-0.0738562724356484
1113	4	0.29742959011365855
1113	5	0.22011335009322863
1113	6	-0.01453964706077926
1113	7	0.0022520927568052684
1113	8	-0.14298922254361202
1113	9	-0.08343593209379208
1113	10	0.25728081434933614
1113	11	-0.1464133064142814
1113	12	-0.18598802888438115
1113	13	-0.30820999202416033
1113	14	0.19373424284212604
1113	15	-0.02084631199078757
1113	16	0.14414527862960824
1113	17	0.3985099318171095
1113	18	0.22691186540043162
1113	19	0.14444677130568095
1113	20	-0.1929018389338708
1113	21	-0.1614344507741663
1113	22	-0.039404348467130035
1113	23	0.14272904598032454
1113	24	-0.04016242983203
1113	25	0.3542243581408826
1114	1	-1.4554302587663663
1114	2	-1.039389285440457
1114	3	-1.0996667414015704
1114	4	-0.8377148746946474
1114	5	-4.0147277469519125
1114	6	-1.7861543620641527
1114	7	-1.566794235516955
1114	8	-1.2385418130360222
1114	9	-0.9485399875540566
1114	10	-0.9369404345701824
1114	11	-1.31132440346736
1114	12	-1.3809043566898396
1114	13	-1.3205815548285074
1114	14	-6.660427697759069
1114	15	-3.1966868240489
1114	16	-2.196764602412447
1114	17	-1.096562207322492
1114	18	-6.769138559369999
1114	19	-3.1004630875116312
1114	20	-2.151906075128931
1114	21	-1.9225745642949903
1114	22	-1.7199768261372164
1114	23	-1.2522678465241412
1114	24	-1.2572165875658001
1114	25	-1.2120091112131774
1115	1	-1.2986027974129115
1115	2	-1.0100563497100588
1115	3	-0.9842112007893284
1115	4	-3.3892519608472185
1115	5	-1.6855343249435428
1115	6	-1.4036160232523365
1115	7	-1.169780732174985
1115	8	-1.0257407808622503
1115	9	-0.8023533855075584
1115	10	-1.2057380569649396
1115	11	-1.3251492901210398
1115	12	-1.374810354019104
1115	13	-1.404924477344153
1115	14	-6.634541533622343
1115	15	-3.366833391399908
1115	16	-2.043523159834262
1115	17	-1.1695274074002526
1115	18	-1.0794267533817106
1115	19	-1.4380156212166781
1115	20	-1.3112182586677008
1115	21	-1.4475654352852985
1115	22	-2.5898197231699847
1115	23	-1.7150118350076582
1115	24	-1.503263126655451
1115	25	-1.0263322023132204
1116	1	-0.969405216282933
1116	2	-0.7130255399116798
1116	3	-0.7458916288705026
1116	4	-0.7682458175539715
1116	5	-1.338518833982004
1116	6	-0.9889795559232475
1116	7	-0.9105880152871997
1116	8	-0.8424761247316148
1116	9	-0.9173551454978224
1116	10	-0.6983491015546406
1116	11	-1.0999843285800708
1116	12	-1.345760458839301
1116	13	-1.3011647150137975
1116	14	-0.46247490804388036
1116	15	-1.014202734868256
1116	16	-0.9365731267544533
1116	17	-0.5740614522635994
1116	18	-0.778013685364769
1116	19	-0.884057750063981
1116	20	-1.3644724384378009
1116	21	-1.5129396489161664
1116	22	-1.5190802164426245
1116	23	-2.2365589826785452
1116	24	-1.828150277020086
1116	25	-1.2536847233567059
1201	1	1.10544476525806
1201	2	1.36525974464438
1201	3	1.3286447441514895
1201	4	1.640051204920944
1201	5	1.5234607444466874
1201	6	1.342278108365754
1201	7	1.2162073934840834
1201	8	1.3505620919735932
1201	9	1.7131445998593051
1201	10	1.975128858554534
1201	11	1.4065827605183259
1201	12	1.40612741466596
1201	13	1.3110760454592594
1201	14	2.266540812838926
1201	15	1.8571719755416538
1201	16	2.0133700662074645
1201	17	2.326851107798319
1201	18	1.7425712972491922
1201	19	1.7121920531953165
1201	20	1.6799796910998097
1201	21	1.6044711267449543
1201	22	1.8495631131041748
1201	23	2.113965105578224
1201	24	1.9258333883656888
1201	25	2.3040659912679615
1202	1	0.9645181901179143
1202	2	1.233836637143522
1202	3	1.103187588757475
1202	4	1.6066775700810467
1202	5	1.3516134048906565
1202	6	1.1372952366524274
1202	7	1.085813195214719
1202	8	1.249788974056294
1202	9	1.4103991907354825
1202	10	1.8149409972067119
1202	11	1.3181085112663595
1202	12	1.176604421025786
1202	13	1.3021560216120989
1202	14	2.103843870917011
1202	15	1.7656975973576579
1202	16	1.8030224626393831
1202	17	2.201142201194642
1202	18	2.264747167566753
1202	19	1.8903949163295763
1202	20	1.533320558935337
1202	21	1.4166389379322482
1202	22	1.7151546318356807
1202	23	1.9998756274508196
1202	24	1.6258400808866824
1202	25	2.0687815450731932
1203	1	0.733804300336395
1203	2	0.8505497129381716
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2211	16	-6.845443819056587
2211	17	-1.9907279444873076
2211	18	-0.7216397783580462
2211	19	-0.6773556572425221
2211	20	-0.6277673153328465
2211	21	-0.6189223696853787
2211	22	-0.4905439723178304
2211	23	-0.07577259271246106
2211	24	-0.5655106078711114
2211	25	-7.447651754642646
2212	1	-1.2289783799060672
2212	2	-3.839424990460813
2212	3	-1.575805552567882
2212	4	-3.5309244102706394
2212	5	-1.1784355705874319
2212	6	-1.0090489431823642
2212	7	-0.8753653714350094
2212	8	-0.7632410547203143
2212	9	-0.8828858739472167
2212	10	-0.32963230841132685
2212	11	-0.7744685869493395
2212	12	-0.8816242959897806
2212	13	-1.0486127969502945
2212	14	-6.4424094698805945
2212	15	-5.1134225333969034
2212	16	-2.7118412724178427
2212	17	-7.371305164377471
2212	18	-3.1816644270008503
2212	19	-2.0172135276611094
2212	20	-1.8650776785095076
2212	21	-1.382123134685969
2212	22	-1.3684337288877444
2212	23	-0.7533999429637248
2212	24	-1.0980866085453656
2212	25	-0.9133971419713944
2213	1	-0.039498297373043556
2213	2	-0.03492754933224518
2213	3	-0.0395002963040092
2213	4	0.4340428141507857
2213	5	0.24752052868402785
2213	6	-0.04853699502051967
2213	7	-0.11084160262020504
2213	8	-0.1607691936122606
2213	9	0.062002106601887874
2213	10	-3.7332019775257734
2213	11	-1.4603561067277013
2213	12	-0.6897521178482791
2213	13	-0.581746849356449
2213	14	0.1831238215293638
2213	15	-0.28589814732319496
2213	16	-0.07935918161992817
2213	17	0.2506112216730543
2213	18	0.42215250791745973
2213	19	-0.05762739541121675
2213	20	-0.47877068348814333
2213	21	-0.4057476326197502
2213	22	-0.6088501323258484
2213	23	-0.9338810136905806
2213	24	-0.2689898061203735
2213	25	0.0519121105367617
2214	1	-1.2571005799675525
2214	2	-1.0251883564694673
2214	3	-0.8828429181662933
2214	4	-0.4730555326423982
2214	5	-3.771982899180422
2214	6	-1.7020908505167363
2214	7	-1.4881683933104881
2214	8	-1.162994255047085
2214	9	-1.0079207238834087
2214	10	-4.719755554933652
2214	11	-2.178082110257723
2214	12	-1.667181276262152
2214	13	-1.34297310543608
2214	14	-0.52573534942686
2214	15	-0.8544010956271393
2214	16	-0.8528516164819848
2214	17	-0.5731668212660566
2214	18	-0.2328618079568443
2214	19	-0.8863938607948896
2214	20	-1.1133952358510442
2214	21	-1.311118710297201
2214	22	-1.007607389580265
2214	23	-0.6473956104908609
2214	24	-1.4223902873834646
2214	25	-0.9744534309125896
2215	1	-2.3703397535446555
2215	2	-4.788689384220273
2215	3	-3.1577094288198175
2215	4	-2.2444773735770305
2215	5	-2.0601508952291425
2215	6	-2.212774962156505
2215	7	-2.384200876630615
2215	8	-2.63713944946711
2215	9	-8.487306578721107
2215	10	-4.207413858414103
2215	11	-3.3802448325775423
2215	12	-3.737258784980771
2215	13	-3.1755356439156017
2215	14	-8.150268759029391
2215	15	-5.049162829571735
2215	16	-9.08817275229153
2215	17	-4.685684036219779
2215	18	-3.271004655901848
2215	19	-3.744367225850606
2215	20	-3.5354704237902332
2215	21	-3.8263336415403124
2215	22	-9.720999743943548
2215	23	-10.954615941312891
2215	24	-7.246733520382801
2215	25	-5.696271896497208
2216	1	-2.422659891608518
2216	2	-2.298545566042691
2216	3	-2.148577368572507
2216	4	-7.359611134510512
2216	5	-4.070590025483629
2216	6	-3.348114738404096
2216	7	-2.806101812239305
2216	8	-8.42193772056283
2216	9	-4.524637003672918
2216	10	-2.9354478047313037
2216	11	-2.808883667401002
2216	12	-3.1390212767387844
2216	13	-2.95170112362095
2216	14	-2.232547919709212
2216	15	-2.869904575899209
2216	16	-2.4396737226607
2216	17	-2.127560599842443
2216	18	-7.836798609858423
2216	19	-5.536576136990167
2216	20	-4.940168722162358
2216	21	-3.977477953534777
2216	22	-3.727764820433947
2216	23	-2.960056187570398
2216	24	-3.3685147272884386
2216	25	-2.730170963234521
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127508.0
3	2.0
4	1045.0
5	5985.0
6	7327.0
7	6398.0
8	6027.0
9	5682.0
10	5381.0
11	5036.0
12	4955.0
13	4833.0
14	4794.0
15	5008.0
16	5095.0
17	5442.0
18	6074.0
19	7482.0
20	9611.0
21	12151.0
22	15014.0
23	18102.0
24	20881.0
25	23786.0
26	26821.0
27	29971.0
28	33406.0
29	39412.0
30	47048.0
31	56155.0
32	71296.0
33	90980.0
34	118565.0
35	155555.0
36	217060.0
37	307559.0
38	725092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.939041500632875	19.109818087838136	12.36417609624927	25.586964315279715
2	14.156663429169535	20.71724256846229	41.746874423278726	23.37921957908945
3	18.03898305998071	25.640167837493642	30.070465163481696	26.250383939043953
4	11.21061064465082	17.27198548350386	39.78897282475217	31.728431047093146
5	12.243932075346308	38.54773939973875	36.73763368338619	12.470694841528749
6	30.793722330686872	38.09716660857661	18.79067237830878	12.31843868242774
7	27.22074731402941	31.820205953531882	23.377150501264698	17.581896231174014
8	24.628483776572395	37.00763908675014	21.35647100354627	17.0074061331312
9	26.572363602153064	14.790080482401615	20.48589597991188	38.15165993553344
10	13.593088138774833	29.152347223808917	36.33036976307118	20.92419487434507
11	33.97276426533987	22.411397846283005	25.217179380693246	18.39865850768388
12	22.590444034645348	24.993413943085717	32.67024733107884	19.745894691190095
13	28.72160596623607	22.122223431885008	25.989470557479816	23.166700044399104
14	20.29895575160974	21.821601443704314	29.20844466666308	28.670998138022863
15	22.866620603003817	30.86601478518112	23.682010168060675	22.585354443754387
16	21.810865515656374	28.0646791444569	28.371471861355495	21.752983478531227
17	21.506791922731377	28.43444108625955	28.556500776315758	21.502266214693318
18	21.34055695095473	27.540953198520747	30.114949429598852	21.00354042092567
19	23.104955647778986	27.28130898527297	27.86051552428541	21.75321984266263
20	23.29185575606113	27.53156517854863	28.152944776562755	21.023634288827488
21	22.947244001700838	27.01899463268217	27.32479598681089	22.708965378806106
22	22.9362439489758	28.014018346091476	27.426066936213477	21.623670768719247
23	22.118477345807808	27.759948493954447	28.038133111944557	22.08344104829319
24	22.70571212066216	27.3717899597276	28.199823172472264	21.72267474713798
25	22.63714628548795	27.31576197658049	27.937005503146768	22.1100862347848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	311.0
3	436.0
4	436.0
5	436.0
6	980.0
7	1524.0
8	1524.0
9	1524.0
10	2487.0
11	3450.0
12	3450.0
13	3450.0
14	6817.5
15	10185.0
16	10185.0
17	10185.0
18	18996.0
19	27807.0
20	27807.0
21	27807.0
22	44244.5
23	60682.0
24	60682.0
25	60682.0
26	86021.0
27	111360.0
28	111360.0
29	111360.0
30	143025.5
31	174691.0
32	174691.0
33	174691.0
34	200762.5
35	226834.0
36	226834.0
37	226834.0
38	245836.0
39	264838.0
40	264838.0
41	264838.0
42	276308.0
43	287778.0
44	287778.0
45	287778.0
46	292179.5
47	296581.0
48	296581.0
49	296581.0
50	281131.0
51	265681.0
52	265681.0
53	265681.0
54	233709.0
55	201737.0
56	201737.0
57	201737.0
58	169852.5
59	137968.0
60	137968.0
61	137968.0
62	110955.5
63	83943.0
64	83943.0
65	83943.0
66	64015.5
67	44088.0
68	44088.0
69	44088.0
70	32100.0
71	20112.0
72	20112.0
73	20112.0
74	14104.0
75	8096.0
76	8096.0
77	8096.0
78	5573.0
79	3050.0
80	3050.0
81	3050.0
82	2044.5
83	1039.0
84	1039.0
85	1039.0
86	685.5
87	332.0
88	332.0
89	332.0
90	215.5
91	99.0
92	99.0
93	99.0
94	62.5
95	26.0
96	26.0
97	26.0
98	21.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17137438584499531
2	0.24680419916516577
3	0.2518657008903316
4	0.42162757291138026
5	0.24823754478645166
6	0.016707434898113764
7	0.017244939506095975
8	0.015005336972836757
9	0.016752226948778946
10	0.019439749988690008
11	0.017827236164743368
12	0.024680419916516573
13	0.022933529940574387
14	0.022530401484587726
15	0.037849282812080776
16	0.018499116924721136
17	0.037804490761415586
18	0.026920022449775795
19	0.023291866345895862
20	0.02553146887915508
21	0.03126485136429868
22	0.03995450919334444
23	0.02544188477782471
24	0.044164961955871766
25	0.03247423673225865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2232539.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.75610444496351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.58422754318572	67.32452240134435
2	9.613762510742756	14.950574437887768
3	2.101603292815737	4.902374554141791
4	0.7090066373244356	2.2051837657588473
5	0.3080005846012671	1.1974462812682973
6	0.183822862404693	0.857600981310688
7	0.10937916668192238	0.5953428536035841
8	0.07166912733273163	0.4458169720290617
9	0.05298971435639129	0.37082463876039096
>10	0.24073243500381405	3.2306821027805737
>50	0.013165204781289777	0.7026773138688281
>100	0.009547838326971073	1.4891822827786747
>500	0.0012791059369625137	0.6210718429010752
>1k	8.139765053370125E-4	1.106699571566156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2724	0.12201354601196217	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2706	0.12120728909998885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.479205066518435E-5	0.0
2	0.0	0.0	0.0	4.479205066518435E-5	0.0
3	0.0	0.0	0.0	4.479205066518435E-5	0.0
4	0.0	0.0	0.0	4.479205066518435E-5	0.0
5	0.0	0.0	0.0	4.479205066518435E-5	0.0
6	0.0	0.0	0.0	4.479205066518435E-5	0.0
7	0.0	0.0	0.0	4.479205066518435E-5	0.0
8	0.0	0.0	0.0	4.479205066518435E-5	0.0
9	0.0	0.0	0.0	8.95841013303687E-5	0.0
10	0.0	0.0	0.0	1.3437615199555304E-4	0.0
11	0.0	0.0	0.0	1.791682026607374E-4	0.0
12	0.0	0.0	0.0	1.791682026607374E-4	0.0
13	0.0	0.0	0.0	1.791682026607374E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	100	1.4621764E-7	12.337824	19
TAAGACG	65	7.838976E-4	11.725182	4
GGCGAGG	660	0.0	11.647596	19
TGGCGAG	1535	0.0	11.438223	18
TAGGACC	675	0.0	11.2909155	4
CGGGCAA	60	0.00592625	11.070917	10
TAGGACT	535	0.0	10.86223	4
CCGAGCA	70	0.0015058573	10.84498	9
GTCCTAA	960	0.0	10.54279	1
AGGACCT	1230	0.0	10.515386	5
GGCGAGA	985	0.0	10.502325	19
GTCTTAA	290	0.0	10.206683	1
GTCCTAC	1525	0.0	10.205605	1
TATGGCG	1665	0.0	10.203373	16
GCGCTAC	75	0.0025394633	10.184733	1
AGGACGT	1450	0.0	10.166118	5
TTAGGAC	1125	0.0	10.16251	3
GTCTATA	275	0.0	10.068998	1
ATGGCGA	1670	0.0	10.05916	17
GACGTGA	850	0.0	10.045529	7
>>END_MODULE