FastQCFastQC Report
Thu 26 May 2016
SRR1513142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232539
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28460.12747817619311466No Hit
CTTTAGGACGTGAAATATGGCGAGG28190.1262687908251547No Hit
GTCCTAAAGTGTGTATTTCTCATTT27330.12241667446794882No Hit
GTATCAACGCAGAGTACTTTTTTTT27010.12098332884666292No Hit
GTACATGGGAAGCAGTGGTATCAAC25390.11372701663890307No Hit
CTGTAGGACGTGGAATATGGCAAGA25220.11296555177759493No Hit
CCCATGTACTCTGCGTTGATACCAC24160.1082175944070854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG500.001477608713.3264574
TAGGACC6050.013.0589834
CCCGCAC550.003022803512.1155053
TGGCCCG550.003023821412.1149615
GGCGAGG7600.011.7361919
GTCCTAC16550.010.8183561
GTCCTAA9750.010.744581
TGGCGAG17100.010.71345918
GGACCTG10350.010.6673256
GGACGTG14600.010.5608796
AGGACGT15050.010.4992495
GGACTGT3100.010.4389086
TCCTACA19100.010.2179962
GACGTGA9250.010.1585757
CCTACAC3200.010.1142843
TTAGGAC11750.010.0459273
GTAGGAC16300.09.9865313
AAATGTC6400.09.9365177
AGGACCG1151.0028123E-59.9327635
TATGGCG18550.09.92696816
Produced by FastQC (version 0.11.5)