##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2232539 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.761357360386537 31.0 31.0 34.0 27.0 34.0 2 30.855348103661346 31.0 31.0 34.0 27.0 34.0 3 30.9605467138536 33.0 31.0 34.0 27.0 34.0 4 34.36933912464687 37.0 35.0 37.0 31.0 37.0 5 34.14949436493607 37.0 35.0 37.0 30.0 37.0 6 34.07525557224308 37.0 35.0 37.0 30.0 37.0 7 33.93299288388691 37.0 35.0 37.0 30.0 37.0 8 33.897271223481425 37.0 35.0 37.0 29.0 37.0 9 35.44345877048509 39.0 35.0 39.0 30.0 39.0 10 35.27354818885583 39.0 35.0 39.0 29.0 39.0 11 35.305883570231025 39.0 35.0 39.0 29.0 39.0 12 35.24446426243841 39.0 35.0 39.0 29.0 39.0 13 35.221229729917376 39.0 35.0 39.0 29.0 39.0 14 36.366511850408884 40.0 36.0 41.0 29.0 41.0 15 36.35329685170114 40.0 36.0 41.0 28.0 41.0 16 36.31995364918597 40.0 36.0 41.0 28.0 41.0 17 36.2498836526484 40.0 36.0 41.0 28.0 41.0 18 36.199062144043175 40.0 36.0 41.0 27.0 41.0 19 36.27641622385992 40.0 37.0 41.0 28.0 41.0 20 36.23836672058137 40.0 37.0 41.0 28.0 41.0 21 36.18550538198885 40.0 37.0 41.0 27.0 41.0 22 36.13166399332778 40.0 36.0 41.0 27.0 41.0 23 36.055814926413376 40.0 36.0 41.0 27.0 41.0 24 36.002815628304816 40.0 36.0 41.0 27.0 41.0 25 35.949142657754244 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46665793825217605 1101 2 0.3761704845898137 1101 3 0.3499549570115228 1101 4 0.4600651108350249 1101 5 0.37207235574911834 1101 6 0.41852542852861063 1101 7 0.4765968689533082 1101 8 0.5208878357423714 1101 9 0.5254300108708492 1101 10 0.644364594313636 1101 11 0.6027704264852574 1101 12 0.6406647935504566 1101 13 0.625456471879339 1101 14 0.7483666943942779 1101 15 0.7174320371293845 1101 16 0.6952335043614752 1101 17 0.6972080308840063 1101 18 0.8002975336236915 1101 19 0.7612074669563427 1101 20 0.7188204416219719 1101 21 0.8440128073276156 1101 22 0.7506398870578721 1101 23 0.8711107556776625 1101 24 0.7581527914256867 1101 25 0.6704665544257651 1102 1 0.518378948013865 1102 2 0.47677929585923806 1102 3 0.5587752064921929 1102 4 0.6807289570304604 1102 5 0.6250524062756853 1102 6 0.5692100663069866 1102 7 0.5696113919060579 1102 8 0.5947940784403443 1102 9 0.6113866665140364 1102 10 0.7996877985082449 1102 11 0.7650851555211773 1102 12 0.8163833585372089 1102 13 0.8261045021769533 1102 14 0.9165738991496752 1102 15 0.7897418454601279 1102 16 0.8000780623089625 1102 17 1.0370924080574326 1102 18 0.9462690155256723 1102 19 1.0094695966778602 1102 20 0.9662005097421229 1102 21 0.9376421893438618 1102 22 0.9273076808134348 1102 23 0.9723637486484762 1102 24 1.0313552908140338 1102 25 1.0091466875138835 1103 1 0.240562703415371 1103 2 0.23460123835554825 1103 3 0.2566081120324064 1103 4 0.30905291858407224 1103 5 0.24059964338126605 1103 6 0.1356563000596367 1103 7 0.15362590351417538 1103 8 0.2423717055363781 1103 9 0.1640887953820993 1103 10 0.20558557353323437 1103 11 0.23250517082976785 1103 12 0.26518414037533944 1103 13 0.2900073277753066 1103 14 0.3917189836760073 1103 15 0.34538821877521286 1103 16 0.35388686357764954 1103 17 0.3746851003231697 1103 18 0.30035360586754933 1103 19 0.29983825607610015 1103 20 0.3692052492751614 1103 21 0.27422458662618254 1103 22 0.32699141142187216 1103 23 0.3300084432474577 1103 24 0.41995787235843096 1103 25 0.4418479328471534 1104 1 0.3041703999684593 1104 2 0.3630810320099158 1104 3 0.32515890036833284 1104 4 0.47488801100824674 1104 5 0.37363680954287304 1104 6 0.3583630053088669 1104 7 0.352575598435223 1104 8 0.511111418322642 1104 9 0.40567394207019447 1104 10 0.49824927622379533 1104 11 0.5741972323069717 1104 12 0.49506591546749945 1104 13 0.5198876847818923 1104 14 0.5275037792638457 1104 15 0.5102670783720669 1104 16 0.530158090878416 1104 17 0.5331087457656167 1104 18 0.36634794043843044 1104 19 0.43053956940946847 1104 20 0.568377495000675 1104 21 0.5863748235749853 1104 22 0.4481548584581958 1104 23 0.49219134491111305 1104 24 0.44979838116044846 1104 25 0.4729884715973611 1105 1 0.29932791250958957 1105 2 0.30449356682256834 1105 3 0.1597985157975792 1105 4 0.2458305135358998 1105 5 0.25200311189266955 1105 6 0.32320601081185885 1105 7 0.3707578971125116 1105 8 0.24597004740637374 1105 9 0.333872588889939 1105 10 0.30652000818339076 1105 11 0.26229152823862734 1105 12 0.3048223489717259 1105 13 0.3744266701399681 1105 14 0.3184289588160141 1105 15 0.3859989688358354 1105 16 0.27486367117622024 1105 17 0.401271241506187 1105 18 0.3422055091068401 1105 19 0.3519903414789738 1105 20 0.4643061057200981 1105 21 0.42484744048012857 1105 22 0.4675549983651095 1105 23 0.3756957348975334 1105 24 0.28161334349986333 1105 25 0.3388639600420902 1106 1 0.35304816313193754 1106 2 0.3719322469835795 1106 3 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-1.0896183769615106 2215 18 -1.0796839193865324 2215 19 -0.9965730301169273 2215 20 -1.075449166067365 2215 21 -1.075632010845645 2215 22 -1.0696617455653055 2215 23 -1.0822254364022186 2215 24 -1.122627018851162 2215 25 -1.0727831431397732 2216 1 -0.5977701962069695 2216 2 -0.5162755289041456 2216 3 -0.5892792854251176 2216 4 -0.3368106630508265 2216 5 -0.6506162723618871 2216 6 -0.8340168079583634 2216 7 -0.9527130956249792 2216 8 -0.8650492741676601 2216 9 -0.9479669920114233 2216 10 -1.0047817314857497 2216 11 -0.9071743586329859 2216 12 -1.057492693233577 2216 13 -1.1220110875122913 2216 14 -1.2542046779652836 2216 15 -1.3250688927924585 2216 16 -1.3784025728890086 2216 17 -1.5194378950695864 2216 18 -1.4172684725169518 2216 19 -1.3765258820870159 2216 20 -1.4879350342970596 2216 21 -1.6884673507540597 2216 22 -1.4950048607353352 2216 23 -1.6032592434462032 2216 24 -1.6595767967326935 2216 25 -1.7749930604167687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50020.0 3 0.0 4 792.0 5 6496.0 6 7380.0 7 5687.0 8 4707.0 9 4135.0 10 3731.0 11 3475.0 12 3189.0 13 2992.0 14 3097.0 15 3141.0 16 3148.0 17 3404.0 18 3652.0 19 4467.0 20 5782.0 21 7508.0 22 9561.0 23 11650.0 24 13939.0 25 15533.0 26 17632.0 27 19472.0 28 22005.0 29 26733.0 30 33849.0 31 43065.0 32 55233.0 33 72422.0 34 95519.0 35 126625.0 36 181803.0 37 293505.0 38 1067190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.00803044343575 20.09073795265202 13.43949344707738 27.46173815683485 2 13.588706733161942 21.163228344798885 40.09561271831922 25.152452203719953 3 16.447629230892588 25.663596305173897 30.530424620109397 27.35834984382412 4 10.930083283351975 17.98769002808064 38.567676850023226 32.51454983854416 5 12.12797442268821 38.80057936269207 36.62321745332351 12.448228761296205 6 30.92057217408265 38.65293980972771 18.277833130423566 12.14865488576607 7 26.95439093598655 31.988490312336037 23.4197590439181 17.637359707759316 8 24.619112534108407 37.48840280599457 20.902141857817842 16.990342802079184 9 26.18604367812179 14.67549594117958 20.169215318609115 38.96924506208951 10 13.782269508629092 29.622817642337022 35.71778603577776 20.87712681325612 11 33.921243928997434 22.414076529010245 25.12309975324059 18.54157978875173 12 22.31235378194961 25.23158609995167 32.66558837270032 19.7904717453984 13 28.651951880795814 22.01063452866893 26.170696234197926 23.166717356337337 14 20.522490013446795 22.032768615660814 28.276346448961746 29.16839492193064 15 22.719423938394804 31.107989602869203 23.532892370525218 22.639694088210778 16 21.864343691196435 28.31654900541491 27.862805532176594 21.956301771212058 17 21.479853033480538 28.80461346918313 28.01037421267136 21.705159284664973 18 21.366145828199475 27.917383913537886 29.573361697628137 21.143108560634506 19 23.053527844306416 27.390115021506904 27.805830043730477 21.750527090456202 20 23.234353352841765 27.516921316940042 28.133483894346302 21.115241435871894 21 22.752735257203312 27.024645758100508 27.40847313835434 22.814145846341837 22 22.803563837911756 28.21606092082685 27.22310985215475 21.757265389106653 23 21.999105002712316 27.94143008740782 27.673423642352272 22.386041267527588 24 22.56533928410657 27.468949030677624 28.176305094782215 21.789406590433583 25 22.495210597110997 27.49495752134459 27.772624189653634 22.23720769189078 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 121.0 1 121.0 2 248.0 3 375.0 4 375.0 5 375.0 6 873.0 7 1371.0 8 1371.0 9 1371.0 10 2290.0 11 3209.0 12 3209.0 13 3209.0 14 6320.5 15 9432.0 16 9432.0 17 9432.0 18 18217.5 19 27003.0 20 27003.0 21 27003.0 22 43466.5 23 59930.0 24 59930.0 25 59930.0 26 86214.0 27 112498.0 28 112498.0 29 112498.0 30 143495.5 31 174493.0 32 174493.0 33 174493.0 34 200003.5 35 225514.0 36 225514.0 37 225514.0 38 244518.5 39 263523.0 40 263523.0 41 263523.0 42 275423.5 43 287324.0 44 287324.0 45 287324.0 46 293532.5 47 299741.0 48 299741.0 49 299741.0 50 284119.0 51 268497.0 52 268497.0 53 268497.0 54 235421.0 55 202345.0 56 202345.0 57 202345.0 58 170345.5 59 138346.0 60 138346.0 61 138346.0 62 110798.5 63 83251.0 64 83251.0 65 83251.0 66 63385.5 67 43520.0 68 43520.0 69 43520.0 70 31741.0 71 19962.0 72 19962.0 73 19962.0 74 13898.0 75 7834.0 76 7834.0 77 7834.0 78 5350.5 79 2867.0 80 2867.0 81 2867.0 82 1945.0 83 1023.0 84 1023.0 85 1023.0 86 646.0 87 269.0 88 269.0 89 269.0 90 172.5 91 76.0 92 76.0 93 76.0 94 44.0 95 12.0 96 12.0 97 12.0 98 7.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18561825795652395 2 0.00895841013303687 3 0.005375046079822122 4 0.0017916820266073738 5 0.08116319580531403 6 0.3319986795303464 7 0.030458594452325356 8 0.014243872111528624 9 0.005240669927826569 10 0.004076076610531776 11 0.0 12 0.0 13 0.0 14 0.0016573058746118209 15 0.0 16 0.0 17 0.020873095609975904 18 0.027995031665740217 19 0.0 20 0.0 21 0.0011198012666296087 22 8.95841013303687E-5 23 0.0058229665864739655 24 0.0 25 2.687523039911061E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2232539.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.67710048921018 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65715279982821 63.21950706763191 2 10.7930006990228 16.119799955620824 3 2.4827935946085713 5.562234802756548 4 0.8606439180534706 2.570815694156263 5 0.4028898636679405 1.504332341760749 6 0.20722659899343243 0.9285048934241887 7 0.13066165395724263 0.683020342385599 8 0.08591403392864704 0.5132648756098389 9 0.060738236513228426 0.40821798524721953 >10 0.288615555354568 3.7117276221102795 >50 0.016739077268997374 0.8704452377829575 >100 0.010985537472758555 1.6610870108676197 >500 0.0017389661039262942 0.8732029160547574 >1k 8.994652261675426E-4 1.373839254591357 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2846 0.12747817619311466 No Hit CTTTAGGACGTGAAATATGGCGAGG 2819 0.1262687908251547 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2733 0.12241667446794882 No Hit GTATCAACGCAGAGTACTTTTTTTT 2701 0.12098332884666292 No Hit GTACATGGGAAGCAGTGGTATCAAC 2539 0.11372701663890307 No Hit CTGTAGGACGTGGAATATGGCAAGA 2522 0.11296555177759493 No Hit CCCATGTACTCTGCGTTGATACCAC 2416 0.1082175944070854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.479205066518435E-5 9 0.0 0.0 0.0 0.0 4.479205066518435E-5 10 4.479205066518435E-5 0.0 0.0 8.95841013303687E-5 4.479205066518435E-5 11 4.479205066518435E-5 0.0 0.0 8.95841013303687E-5 4.479205066518435E-5 12 4.479205066518435E-5 0.0 0.0 8.95841013303687E-5 4.479205066518435E-5 13 4.479205066518435E-5 0.0 0.0 8.95841013303687E-5 4.479205066518435E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACACG 50 0.0014776087 13.326457 4 TAGGACC 605 0.0 13.058983 4 CCCGCAC 55 0.0030228035 12.115505 3 TGGCCCG 55 0.0030238214 12.114961 5 GGCGAGG 760 0.0 11.73619 19 GTCCTAC 1655 0.0 10.818356 1 GTCCTAA 975 0.0 10.74458 1 TGGCGAG 1710 0.0 10.713459 18 GGACCTG 1035 0.0 10.667325 6 GGACGTG 1460 0.0 10.560879 6 AGGACGT 1505 0.0 10.499249 5 GGACTGT 310 0.0 10.438908 6 TCCTACA 1910 0.0 10.217996 2 GACGTGA 925 0.0 10.158575 7 CCTACAC 320 0.0 10.114284 3 TTAGGAC 1175 0.0 10.045927 3 GTAGGAC 1630 0.0 9.986531 3 AAATGTC 640 0.0 9.936517 7 AGGACCG 115 1.0028123E-5 9.932763 5 TATGGCG 1855 0.0 9.926968 16 >>END_MODULE