FastQCFastQC Report
Thu 26 May 2016
SRR1513141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745470
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT41740.15203225677206453No Hit
GTCCTAAAGTGTGTATTTCTCATTT39080.1423435695891778No Hit
CTGTAGGACGTGGAATATGGCAAGA36380.13250918786218754No Hit
GTACATGGGAAGCAGTGGTATCAAC32750.11928740798478948No Hit
CTTTAGGACGTGAAATATGGCGAGG32600.11874105344440114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT5100.012.7060044
TAGGACC7450.012.6633674
ATAGGAC3000.011.4354043
TTAGGAC14750.011.30619053
GGCGAGG10800.010.98289819
GGACGTG21150.010.9453846
AGGACGT21500.010.8814265
GACGTGA12100.010.7426267
TGGCGAG22800.010.73760918
GTCCTAC25250.010.6686171
GTCCTAA11950.010.6317351
GACGTGG10050.010.4792987
GTCTTAT2650.010.4537611
AGGACCT16850.010.3285725
GTCCTAG4100.010.2515421
GGACCTG15950.010.1715466
GTAGGAC22750.010.1368783
TCCTACA27900.010.1173732
GTATTAC2650.010.09328651
TTTAGGA18200.010.0602812
Produced by FastQC (version 0.11.5)