##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513141_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2745470
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.387725598895635	31.0	30.0	34.0	25.0	34.0
2	29.23972725981344	31.0	30.0	34.0	22.0	34.0
3	29.42878195718766	31.0	30.0	34.0	23.0	34.0
4	32.50029430297909	35.0	33.0	37.0	22.0	37.0
5	32.654456249749586	37.0	33.0	37.0	25.0	37.0
6	33.03903338954714	37.0	35.0	37.0	26.0	37.0
7	32.98846172057972	37.0	35.0	37.0	26.0	37.0
8	32.86421232065912	37.0	35.0	37.0	26.0	37.0
9	34.110754807009364	39.0	35.0	39.0	24.0	39.0
10	33.687912452148446	39.0	34.0	39.0	18.0	39.0
11	34.14488375396562	39.0	35.0	39.0	25.0	39.0
12	34.14033225640783	39.0	35.0	39.0	25.0	39.0
13	34.184855780613155	39.0	35.0	39.0	25.0	39.0
14	34.3981660699261	39.0	34.0	41.0	17.0	41.0
15	34.836172312937315	40.0	34.0	41.0	21.0	41.0
16	34.68562176967878	39.0	34.0	41.0	18.0	41.0
17	34.293747882876154	39.0	33.0	41.0	17.0	41.0
18	34.02900814796738	39.0	33.0	41.0	16.0	41.0
19	34.66199739935239	39.0	34.0	41.0	18.0	41.0
20	34.885431638298726	40.0	35.0	41.0	22.0	41.0
21	34.921161404058324	40.0	35.0	41.0	22.0	41.0
22	34.61012285692431	40.0	34.0	41.0	18.0	41.0
23	34.156982957380706	39.0	34.0	41.0	10.0	41.0
24	34.40433040608712	39.0	34.0	41.0	16.0	41.0
25	34.04331389525291	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1995513113767693
1101	2	1.4418808549994075
1101	3	1.2983433375976894
1101	4	1.7438826450729437
1101	5	1.5739111111948745
1101	6	1.339237189426953
1101	7	1.2944545806260734
1101	8	1.351710578584374
1101	9	1.6601856963364128
1101	10	1.9448406712146067
1101	11	1.548269802440629
1101	12	1.574807920283284
1101	13	1.452020103304406
1101	14	2.490001380155043
1101	15	2.1184298966954103
1101	16	2.130527450938537
1101	17	2.5276939282062827
1101	18	2.5624542236729155
1101	19	2.131086943075587
1101	20	1.7365977251547022
1101	21	1.7249500154952244
1101	22	2.061408140097683
1101	23	2.2422220385874496
1101	24	1.9271782234402153
1101	25	2.235708292908022
1102	1	0.8355460755187423
1102	2	1.047078229884594
1102	3	0.9265263540795701
1102	4	1.1347647314579987
1102	5	1.198423747694541
1102	6	0.8992618460116617
1102	7	0.8999612015838778
1102	8	0.9845910679618584
1102	9	1.2795425818245718
1102	10	1.4530593698416325
1102	11	1.1390037366763792
1102	12	0.9983124426939369
1102	13	1.0492463719526626
1102	14	2.0124890261991126
1102	15	1.5691626161886347
1102	16	1.6462425407781538
1102	17	2.110194193396552
1102	18	2.3320182386487076
1102	19	1.66640906493015
1102	20	1.523887754342745
1102	21	1.438045795377036
1102	22	1.6131888011033695
1102	23	1.95713033896957
1102	24	1.7827219954772815
1102	25	1.977424274373547
1103	1	0.8748087908899329
1103	2	1.140182993388585
1103	3	0.8764791568286476
1103	4	1.4274250491848548
1103	5	1.1908870603778254
1103	6	0.9537530212651859
1103	7	0.9333669600083212
1103	8	0.8523588838745866
1103	9	1.2870731520897039
1103	10	1.522664460188082
1103	11	1.1840221178166672
1103	12	1.063978991417457
1103	13	1.0600283950175395
1103	14	1.8943731980282905
1103	15	1.5488432701134442
1103	16	1.6163323774768656
1103	17	1.9211378985306737
1103	18	2.190117754681978
1103	19	1.5171549254253875
1103	20	1.2609428737751784
1103	21	1.2833629525072965
1103	22	1.5654579873556003
1103	23	1.889552931280079
1103	24	1.5918278864378408
1103	25	1.7595396947409299
1104	1	0.9481543871450242
1104	2	1.2499046604384674
1104	3	1.1027443307204123
1104	4	1.4190324352369572
1104	5	1.3455291068708064
1104	6	1.1213739554875843
1104	7	1.0237623213223372
1104	8	1.1119880484040863
1104	9	1.456945865320094
1104	10	1.6430130101565439
1104	11	1.260532844925386
1104	12	1.2300372285045356
1104	13	1.1131152318322464
1104	14	2.1503561478505375
1104	15	1.7285545608449127
1104	16	1.8031398908746965
1104	17	2.269677667539682
1104	18	2.2961524092272185
1104	19	1.6380182822823102
1104	20	1.4315270133366553
1104	21	1.414569730129827
1104	22	1.7331868706632818
1104	23	2.0035428615347115
1104	24	1.7758614443948844
1104	25	2.0455758821513896
1105	1	0.7276247768415303
1105	2	0.9778368393157848
1105	3	0.8248627065254617
1105	4	1.1788855174256838
1105	5	1.081429962810958
1105	6	0.8088783903009542
1105	7	0.7917911699329494
1105	8	0.77130619646924
1105	9	0.9956577636666708
1105	10	1.2688062759913592
1105	11	0.8495796048967321
1105	12	0.9034188231194662
1105	13	0.8495923288061888
1105	14	1.6574055081774333
1105	15	1.2336748248073377
1105	16	1.3979357457937098
1105	17	1.6692194429122011
1105	18	1.399359765536495
1105	19	1.1867673568480868
1105	20	1.0582993107606598
1105	21	1.141843556400076
1105	22	1.1647337049496898
1105	23	1.4412038543851011
1105	24	1.2142495860353648
1105	25	1.399202863056594
1106	1	0.5947582063376764
1106	2	0.7444659367256321
1106	3	0.6325968808775322
1106	4	1.1439538758944394
1106	5	1.0131626577530852
1106	6	0.7447725535054701
1106	7	0.6553794351729536
1106	8	0.6573107460110492
1106	9	0.8221863777687304
1106	10	0.8124594689431035
1106	11	0.7572696106976906
1106	12	0.6849562519514834
1106	13	0.5922063606695716
1106	14	1.5649077552955433
1106	15	1.1704206473810501
1106	16	1.2363040194702961
1106	17	1.542580737397067
1106	18	1.6570419427055825
1106	19	1.1398311901865839
1106	20	0.889830131538254
1106	21	0.7790850794167454
1106	22	1.0174508300541447
1106	23	1.5126074010561013
1106	24	1.05964196167794
1106	25	1.3851939628862198
1107	1	0.6060767147222919
1107	2	0.8392917582002433
1107	3	0.7883015091731522
1107	4	1.1391448149647445
1107	5	1.0243920629136767
1107	6	0.8402044482558324
1107	7	0.8219408914943287
1107	8	0.846415318011708
1107	9	1.1130436901504694
1107	10	1.2862125721342323
1107	11	0.831318673674474
1107	12	0.7821414239869569
1107	13	0.7408889695023007
1107	14	1.5461907092090712
1107	15	1.0643075607676877
1107	16	1.2404867875464873
1107	17	1.6152381336729178
1107	18	1.7325475578079903
1107	19	1.2501260167620103
1107	20	1.0868910502701752
1107	21	0.9262314572793002
1107	22	1.2376023923535229
1107	23	1.53337399264759
1107	24	1.3010752007446342
1107	25	1.6618208049811471
1108	1	0.38529516003429265
1108	2	0.6193703681208227
1108	3	0.4899912485777733
1108	4	0.973954708571398
1108	5	0.7950582687487255
1108	6	0.4321691198598927
1108	7	0.37505199449464044
1108	8	0.4796545933280285
1108	9	0.612601645660483
1108	10	0.8904130574762732
1108	11	0.5900712658522949
1108	12	0.3496400319223767
1108	13	0.33428988232278556
1108	14	1.2269400027168231
1108	15	0.7839118694690868
1108	16	0.9035171908601995
1108	17	1.1893121047135224
1108	18	1.5039787871467922
1108	19	0.9252758946030539
1108	20	0.7740228096840269
1108	21	0.6310166765133403
1108	22	0.9635798754028286
1108	23	1.253210089561101
1108	24	0.9074528038831531
1108	25	1.1125441110577583
1109	1	0.5262090398640673
1109	2	0.7750195464800136
1109	3	0.6784243825190899
1109	4	0.9172639075923428
1109	5	1.0201387547851297
1109	6	0.6336827091253951
1109	7	0.6123473187155
1109	8	0.5846005732101958
1109	9	0.8134217619841166
1109	10	1.2020915891474644
1109	11	0.8114699024079357
1109	12	0.7518956772950887
1109	13	0.6851233740391649
1109	14	1.6270035636514137
1109	15	1.213645935058807
1109	16	1.2419045569226697
1109	17	1.7059753737485295
1109	18	1.8655815842730163
1109	19	1.3009294194085115
1109	20	1.0779118442210276
1109	21	1.0795868448029324
1109	22	1.2772999348866065
1109	23	1.6293097204358702
1109	24	1.4248937262644787
1109	25	1.5524162681191385
1110	1	0.48529260857257484
1110	2	0.6915367031891115
1110	3	0.605658136334462
1110	4	0.9249439883630401
1110	5	0.8962738752922164
1110	6	0.6018998867600018
1110	7	0.5358307050100848
1110	8	0.6318482950227846
1110	9	0.7703087042023427
1110	10	1.0404858566178348
1110	11	0.6335312371021615
1110	12	0.6138918427178481
1110	13	0.5105244021261441
1110	14	1.2346869333468646
1110	15	0.8871781033917117
1110	16	1.0738902772501646
1110	17	1.3785046407641914
1110	18	1.020394693882949
1110	19	0.751808665984278
1110	20	0.6535643977951793
1110	21	0.4958428741304317
1110	22	0.8158573904154878
1110	23	-0.31144625988885366
1110	24	0.277944016352059
1110	25	0.8091210392232213
1111	1	-0.0112845405636115
1111	2	0.395028615833251
1111	3	0.28209878181102255
1111	4	0.6791478745055954
1111	5	0.5383620357056103
1111	6	0.20567463412293563
1111	7	0.12940671751572097
1111	8	0.2510110761768587
1111	9	0.4646877016456017
1111	10	0.7033327246741976
1111	11	0.2286407559105612
1111	12	0.09197560779980307
1111	13	-0.021174199089323054
1111	14	0.930394627606745
1111	15	0.5641893860023472
1111	16	0.5820633798735955
1111	17	0.9617286217789527
1111	18	1.1619265802846854
1111	19	0.6631298302611413
1111	20	0.29090057855453466
1111	21	0.16821562415540114
1111	22	0.48097892348981475
1111	23	0.8363779700506981
1111	24	0.5025483150294079
1111	25	0.6879423452414244
1112	1	0.1925303271011103
1112	2	0.3919737656896132
1112	3	0.31147433774279776
1112	4	0.6081612921121362
1112	5	0.36162058193897906
1112	6	0.17005377757818962
1112	7	0.0043273754702113365
1112	8	0.12156957430784843
1112	9	0.27891358932252786
1112	10	0.6010701931993907
1112	11	0.13682864002414874
1112	12	0.17795207257086076
1112	13	0.12252785641383213
1112	14	0.6882170142907214
1112	15	0.40153311374827894
1112	16	0.3203304044864268
1112	17	0.808736519705171
1112	18	0.8668074395704579
1112	19	0.30677109941139236
1112	20	0.15581094421320074
1112	21	0.14001725963614575
1112	22	0.4548787899318256
1112	23	0.8222330233529647
1112	24	0.5352298208755997
1112	25	0.8998578677971381
1113	1	-0.12331688049195577
1113	2	0.04699530045463263
1113	3	-0.02056484059324859
1113	4	0.20109636322762015
1113	5	0.299072774502001
1113	6	-0.02932012685155172
1113	7	-0.18077655458399278
1113	8	-0.05172854559497608
1113	9	0.00921207584333672
1113	10	0.36195412401657023
1113	11	0.07351656018807518
1113	12	-0.08204247018797872
1113	13	-0.2621361967257343
1113	14	0.4023128612914064
1113	15	0.11110216627681524
1113	16	0.2558783819772472
1113	17	0.5722394736632594
1113	18	0.4025523691532982
1113	19	0.2735326858650211
1113	20	0.07110344005399583
1113	21	0.061299788899304986
1113	22	0.18514624001002744
1113	23	0.21561476060490747
1113	24	0.1712564061750541
1113	25	0.4864876916200629
1114	1	-0.7796434017852079
1114	2	-0.459977828739472
1114	3	-0.4972167492138624
1114	4	-0.2622323974580141
1114	5	-3.5200070261280203
1114	6	-1.231153054608157
1114	7	-0.9557201575386429
1114	8	-0.5761633542889726
1114	9	-0.23692004133060607
1114	10	-0.1250645667601873
1114	11	-0.5330067879033038
1114	12	-0.6718689949693868
1114	13	-0.6027537388229476
1114	14	-6.016008615502567
1114	15	-2.2743523745653036
1114	16	-1.136651395726119
1114	17	-0.3364385364675968
1114	18	-6.429949198928732
1114	19	-2.4286902804958928
1114	20	-1.44359562455098
1114	21	-1.049474345769923
1114	22	-0.7273791997030017
1114	23	-0.31927593662556575
1114	24	-0.4666555364264795
1114	25	-0.35092931418992634
1115	1	-0.8879516209957892
1115	2	-0.47952974493870926
1115	3	-0.5943750146752116
1115	4	-3.2070330143906887
1115	5	-1.140237525873573
1115	6	-0.7767082713043436
1115	7	-0.7591540311524483
1115	8	-0.5095782965333058
1115	9	-0.31404727642696173
1115	10	-0.5843320894320456
1115	11	-0.7321033053813295
1115	12	-0.7292233822711012
1115	13	-0.8561223525647605
1115	14	-6.072870007855176
1115	15	-2.5805827465066997
1115	16	-1.419041143692482
1115	17	-0.5977902158556248
1115	18	-0.38166537355444063
1115	19	-0.6673260326894805
1115	20	-0.7048461508850536
1115	21	-0.8211155217660462
1115	22	-1.9983283050789424
1115	23	-1.3342961051513953
1115	24	-0.9476677857382754
1115	25	-0.5778923370954416
1116	1	-0.35336416916192803
1116	2	-0.14806410976351003
1116	3	-0.19628358525060108
1116	4	0.008906290575339426
1116	5	-0.609019956881717
1116	6	-0.49828956037325156
1116	7	-0.3769142165576014
1116	8	-0.23205303574295044
1116	9	-0.34157120412130126
1116	10	-0.05232500581953303
1116	11	-0.331159188398388
1116	12	-0.7378988953016119
1116	13	-0.579758494213479
1116	14	0.2344392380574618
1116	15	-0.19270867057208108
1116	16	-0.2251024738317824
1116	17	0.29995322954113135
1116	18	0.09037928130057082
1116	19	-0.0984878506867588
1116	20	-0.5387848630370726
1116	21	-0.692205275351057
1116	22	-0.6424335935535694
1116	23	-1.2553713050054753
1116	24	-1.019538899073865
1116	25	-0.38854494183632937
1201	1	0.7348126527791194
1201	2	0.9687848688185241
1201	3	0.9586874333399109
1201	4	1.197727242013812
1201	5	1.1354974319639766
1201	6	0.8501986350038564
1201	7	0.8384848079705662
1201	8	0.8643145686961589
1201	9	1.0075134074571253
1201	10	1.3998114895505083
1201	11	0.9021544116581595
1201	12	0.9550483726703973
1201	13	0.7700329810109494
1201	14	1.6305214462994897
1201	15	1.3663807123359604
1201	16	1.5203956883227434
1201	17	1.7797192576743015
1201	18	1.2150479389665065
1201	19	1.168587324261992
1201	20	1.1209133061860825
1201	21	1.0174018851776765
1201	22	1.2923935782849867
1201	23	1.6026904748771855
1201	24	1.174746991927762
1201	25	1.6163456710144501
1202	1	0.7060634098035301
1202	2	0.9124285738097484
1202	3	0.8099628738565947
1202	4	1.1627244975928548
1202	5	1.1262123925528371
1202	6	0.7876251733070276
1202	7	0.7242852089566014
1202	8	0.8362040810969873
1202	9	1.1103143947485279
1202	10	1.2319351329875161
1202	11	0.8645274165510415
1202	12	0.7776337198164072
1202	13	0.8219965728598453
1202	14	1.7232108086293252
1202	15	1.3149993819551185
1202	16	1.3971040707512543
1202	17	1.8882761654488434
1202	18	1.957940721616211
1202	19	1.5137987231392884
1202	20	1.2868575227972485
1202	21	1.2339059165853925
1202	22	1.404747106057954
1202	23	1.7019819591809906
1202	24	1.282257675976041
1202	25	1.6665616334648305
1203	1	0.8909622890185069
1203	2	0.9488637109353419
1203	3	0.940599233683475
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2211	14	-0.4497681658343282
2211	15	-0.7107734546252331
2211	16	-7.004778985028224
2211	17	-2.336178495876311
2211	18	-1.0603331706992591
2211	19	-1.2341206979059862
2211	20	-1.3725262877253428
2211	21	-1.2065325178759778
2211	22	-1.2342110021914436
2211	23	-0.8015593362433435
2211	24	-1.254693901878369
2211	25	-8.092311067737011
2212	1	-1.6836387620970044
2212	2	-4.146269540122518
2212	3	-1.906301562372267
2212	4	-3.8570853553725435
2212	5	-1.5106764054940136
2212	6	-1.156641839539077
2212	7	-1.0992659962963955
2212	8	-0.8532066315049889
2212	9	-0.9736754691755962
2212	10	-0.40669165279929587
2212	11	-0.9270980042722883
2212	12	-1.0386930818359161
2212	13	-1.2102612907796981
2212	14	-6.3293798252710864
2212	15	-5.073918441556081
2212	16	-2.8436397999047216
2212	17	-7.3214688879844445
2212	18	-3.1103506570046235
2212	19	-2.05918053271121
2212	20	-2.0522027900284243
2212	21	-1.642122565957557
2212	22	-1.6851573057184979
2212	23	-1.1924288655652262
2212	24	-1.5429017934092144
2212	25	-1.308840037170235
2213	1	-0.31442280850570725
2213	2	-0.3044069051652656
2213	3	-0.3664185336526842
2213	4	0.019056821254785916
2213	5	-0.10610085242165468
2213	6	-0.3308973132912527
2213	7	-0.463101850697484
2213	8	-0.4729098404940757
2213	9	-0.2933822763583862
2213	10	-4.1747259995572215
2213	11	-1.785073500573148
2213	12	-1.1651597955456339
2213	13	-0.9030635540435412
2213	14	-0.07625814235591122
2213	15	-0.6416520892649302
2213	16	-0.5208206551154078
2213	17	-0.03260049991525449
2213	18	0.08797983109035101
2213	19	-0.5339577445508681
2213	20	-0.9315662995466454
2213	21	-0.8842822018203478
2213	22	-1.1120555028235515
2213	23	-1.3061474788213658
2213	24	-0.7511183686884237
2213	25	-0.48267916992559634
2214	1	-1.0567738697459
2214	2	-0.8201831848960879
2214	3	-0.7569947756924016
2214	4	-0.2899474268469149
2214	5	-3.573000013009324
2214	6	-1.5447199373774403
2214	7	-1.2557085047481031
2214	8	-0.8705419753025936
2214	9	-0.7570857415844188
2214	10	-4.296235057201056
2214	11	-1.9449968619915836
2214	12	-1.4154927336458485
2214	13	-1.0363524563686113
2214	14	-0.4009660362868601
2214	15	-0.7500369861247904
2214	16	-0.7572997561825332
2214	17	-0.42875129574724724
2214	18	-0.15415627306273194
2214	19	-0.8777239890248012
2214	20	-1.1721653132960483
2214	21	-1.1317765285237158
2214	22	-0.7538427679412223
2214	23	-0.500599426587776
2214	24	-0.9645733081863526
2214	25	-0.6588795923800674
2215	1	-2.478364396390198
2215	2	-5.085871595062748
2215	3	-3.1988186347572807
2215	4	-2.220299595691273
2215	5	-2.205132562851894
2215	6	-2.327856766861583
2215	7	-2.4566002634327937
2215	8	-2.6047447146072287
2215	9	-8.45761613787608
2215	10	-4.265457400281996
2215	11	-3.4059279699967497
2215	12	-3.721088456761951
2215	13	-3.087979067788538
2215	14	-8.024671797674458
2215	15	-5.013700009357436
2215	16	-9.663314607239862
2215	17	-4.941323386160963
2215	18	-3.485888843335413
2215	19	-3.8682538473754065
2215	20	-3.529629381454928
2215	21	-3.7444901828855137
2215	22	-9.769499280339208
2215	23	-10.777058698526307
2215	24	-6.972328544226926
2215	25	-5.289944368057675
2216	1	-1.8224261441782659
2216	2	-1.7887868893071506
2216	3	-1.6957280220917532
2216	4	-6.6776813284494665
2216	5	-3.367587733020983
2216	6	-2.6671889294519495
2216	7	-2.162059680869831
2216	8	-7.834356847958325
2216	9	-3.9108093426981725
2216	10	-2.241469070745577
2216	11	-2.2564249947904003
2216	12	-2.3929525099263955
2216	13	-2.356698389037117
2216	14	-1.5350915227775204
2216	15	-2.106933525244628
2216	16	-1.7254377363107807
2216	17	-1.4756315872898256
2216	18	-7.3757499703186475
2216	19	-4.795596826602946
2216	20	-4.181123138131081
2216	21	-3.317066577019716
2216	22	-2.946345031910436
2216	23	-2.220575174017455
2216	24	-2.6290040444355363
2216	25	-2.1264767328795777
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129075.0
3	2.0
4	1101.0
5	6401.0
6	7450.0
7	6635.0
8	6273.0
9	5827.0
10	5551.0
11	5307.0
12	5144.0
13	5137.0
14	5073.0
15	5350.0
16	5156.0
17	5642.0
18	6415.0
19	7888.0
20	10015.0
21	12838.0
22	16029.0
23	19296.0
24	22218.0
25	25638.0
26	28846.0
27	32437.0
28	36205.0
29	43162.0
30	52072.0
31	61990.0
32	79486.0
33	103328.0
34	137046.0
35	183080.0
36	262159.0
37	382334.0
38	1017864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13457058957459	18.740732254090943	12.305158412778106	25.819538743556365
2	14.154963008406185	20.392446849689243	41.79400100786573	23.65858913403884
3	18.205717069372938	25.25245295572538	29.785231031930326	26.75659894297136
4	11.252222978461807	17.043456941913483	39.358830804809685	32.34548927481502
5	12.425598389784833	38.322836163820114	36.460805779480566	12.79075966691449
6	31.43139839285623	37.48008192367634	18.543865669651737	12.544654013815695
7	27.824576218393748	31.487544385456108	22.956720742211164	17.73115865393898
8	24.911158299062567	36.89406863479039	21.080810520460275	17.113962545686764
9	26.551526034147287	14.868646199568596	20.403236294763808	38.176591471520304
10	13.756795718064632	28.997383172667252	35.967841767741	21.277979341527114
11	34.13217718100071	22.22911969433512	25.056038925058115	18.582664199606043
12	23.087194381739195	24.715567531489047	32.22411835771673	19.973119729055032
13	28.860375890186173	21.906221639461805	25.84036453048702	23.393037939865003
14	20.250827371835694	21.819154222693086	28.922165112961213	29.007853292510006
15	23.00562596104095	30.892611189249457	23.337171424198917	22.764591425510673
16	21.94728946277424	27.8192275717553	28.229070505370945	22.004412460099513
17	21.498109627055797	28.388970832580295	28.471392258944654	21.64152728141925
18	21.37588252482437	27.33533429373603	30.129428472952423	21.15935470848718
19	23.386690138997782	27.00900468960854	27.594149210577708	22.010155960815975
20	23.64503411437963	27.362913095134434	27.8222285049533	21.16982428553264
21	23.05942287043241	26.75615931454797	27.09256743191953	23.091850383100095
22	23.225598633402495	27.93821743271774	27.11521265268732	21.72097128119245
23	22.045702706158945	27.63180761449449	27.98845354694927	22.33403613239729
24	23.025009939039347	27.147588630540675	27.918041825226688	21.9093596051933
25	22.650999253065166	27.200051010183817	27.854730283653055	22.29421945309796
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	185.0
1	185.0
2	313.0
3	441.0
4	441.0
5	441.0
6	995.5
7	1550.0
8	1550.0
9	1550.0
10	2595.0
11	3640.0
12	3640.0
13	3640.0
14	7123.5
15	10607.0
16	10607.0
17	10607.0
18	20415.0
19	30223.0
20	30223.0
21	30223.0
22	49473.0
23	68723.0
24	68723.0
25	68723.0
26	99810.0
27	130897.0
28	130897.0
29	130897.0
30	170011.5
31	209126.0
32	209126.0
33	209126.0
34	241206.0
35	273286.0
36	273286.0
37	273286.0
38	297344.5
39	321403.0
40	321403.0
41	321403.0
42	336323.5
43	351244.0
44	351244.0
45	351244.0
46	359328.5
47	367413.0
48	367413.0
49	367413.0
50	349392.0
51	331371.0
52	331371.0
53	331371.0
54	292582.5
55	253794.0
56	253794.0
57	253794.0
58	215835.0
59	177876.0
60	177876.0
61	177876.0
62	143972.5
63	110069.0
64	110069.0
65	110069.0
66	84611.0
67	59153.0
68	59153.0
69	59153.0
70	43231.5
71	27310.0
72	27310.0
73	27310.0
74	19068.0
75	10826.0
76	10826.0
77	10826.0
78	7529.5
79	4233.0
80	4233.0
81	4233.0
82	2862.5
83	1492.0
84	1492.0
85	1492.0
86	976.0
87	460.0
88	460.0
89	460.0
90	283.0
91	106.0
92	106.0
93	106.0
94	69.5
95	33.0
96	33.0
97	33.0
98	21.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1741778274758056
2	0.25532968854148835
3	0.25638597398623914
4	0.4607589957275075
5	0.2532899649240385
6	0.017337650748323604
7	0.01759261620050483
8	0.015261503494847877
9	0.01744692165640127
10	0.019778034362058227
11	0.01832108892102263
12	0.025715087034278286
13	0.024731648861579254
14	0.02309258524041421
15	0.03824481782718441
16	0.0187945961893592
17	0.03780773419487374
18	0.028701825188401257
19	0.02469522522555337
20	0.02669852520697731
21	0.03230776515496436
22	0.04137725052541095
23	0.02662567793492553
24	0.04520173230812939
25	0.03350974514381873
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2745470.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.27331330081843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29078737590655	60.19701171090747
2	11.41149186898297	16.494926541534966
3	2.815514371161042	6.1045965674963645
4	1.0072251591815473	2.911819979759787
5	0.480335994926092	1.7357736925476892
6	0.2538775097353125	1.1009141280679091
7	0.16313439062875648	0.8253184046835152
8	0.11397169285646458	0.658968949219193
9	0.07919677728847367	0.51514321476465
>10	0.35148432708392585	4.383928629850466
>50	0.017645695695436656	0.8771482297211576
>100	0.012380297301051361	1.7094624656831474
>500	0.001986672945704072	0.9888112641498198
>1k	9.678663067648776E-4	1.4961762216138415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4174	0.15203225677206453	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3908	0.1423435695891778	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3638	0.13250918786218754	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3275	0.11928740798478948	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3260	0.11874105344440114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.642363602588992E-5	0.0
9	0.0	0.0	0.0	3.642363602588992E-5	0.0
10	0.0	0.0	0.0	7.284727205177984E-5	0.0
11	0.0	0.0	0.0	7.284727205177984E-5	0.0
12	0.0	0.0	0.0	7.284727205177984E-5	0.0
13	0.0	0.0	0.0	7.284727205177984E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACT	510	0.0	12.706004	4
TAGGACC	745	0.0	12.663367	4
ATAGGAC	300	0.0	11.435404	3
TTAGGAC	1475	0.0	11.3061905	3
GGCGAGG	1080	0.0	10.982898	19
GGACGTG	2115	0.0	10.945384	6
AGGACGT	2150	0.0	10.881426	5
GACGTGA	1210	0.0	10.742626	7
TGGCGAG	2280	0.0	10.737609	18
GTCCTAC	2525	0.0	10.668617	1
GTCCTAA	1195	0.0	10.631735	1
GACGTGG	1005	0.0	10.479298	7
GTCTTAT	265	0.0	10.453761	1
AGGACCT	1685	0.0	10.328572	5
GTCCTAG	410	0.0	10.251542	1
GGACCTG	1595	0.0	10.171546	6
GTAGGAC	2275	0.0	10.136878	3
TCCTACA	2790	0.0	10.117373	2
GTATTAC	265	0.0	10.0932865	1
TTTAGGA	1820	0.0	10.060281	2
>>END_MODULE