FastQCFastQC Report
Thu 26 May 2016
SRR1513141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745470
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43740.1593169839772425No Hit
GTCCTAAAGTGTGTATTTCTCATTT39800.14496607138304188No Hit
CTGTAGGACGTGGAATATGGCAAGA39770.14485680047496421No Hit
CTTTAGGACGTGAAATATGGCGAGG39490.14383693866623928No Hit
GTACATGGGAAGCAGTGGTATCAAC36600.1333105078547571No Hit
CCCATGTACTCTGCGTTGATACCAC36240.13199925695782508No Hit
GTATCAACGCAGAGTACTTTTTTTT29500.10744972627637527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.013624
GGCGAGG9650.010.91482919
GTCTTAC2200.010.3893111
ATTTAGA8550.010.35893251
GCGCCAC1409.831092E-810.16902713
TGGCGAG22950.010.09056318
AATCCCG1701.5916157E-910.04719519
AATGTCC8050.010.0203728
CGGGACT1054.012435E-59.9735724
TTTAGAA9900.09.9059712
AAATGTC8050.09.9041097
GTATTAA3500.09.7956351
CACCTTT9050.09.75327714
AGGACCT17500.09.6290315
AGGACGT24100.09.5992345
TAGAAAT8750.09.574634
GGACGTG23800.09.5597056
GACGTGG11750.09.5318277
GTCTAGG2600.09.5235351
GTCCTAA13400.09.5235341
Produced by FastQC (version 0.11.5)