##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513141_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2745470
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7155314026378	31.0	31.0	34.0	27.0	34.0
2	30.82443625317341	33.0	31.0	34.0	27.0	34.0
3	30.936118405955995	33.0	31.0	34.0	27.0	34.0
4	34.32805894801255	37.0	35.0	37.0	31.0	37.0
5	34.0651684411048	37.0	35.0	37.0	30.0	37.0
6	33.979096839521105	37.0	35.0	37.0	30.0	37.0
7	33.85202351509942	37.0	35.0	37.0	29.0	37.0
8	33.81579875212623	37.0	35.0	37.0	29.0	37.0
9	35.365695491118096	39.0	35.0	39.0	29.0	39.0
10	35.211022520734154	39.0	35.0	39.0	28.0	39.0
11	35.22613541579402	39.0	35.0	39.0	29.0	39.0
12	35.16143866077575	39.0	35.0	39.0	28.0	39.0
13	35.13300892014846	39.0	35.0	39.0	28.0	39.0
14	36.29149945182428	40.0	37.0	41.0	27.0	41.0
15	36.27727602195617	40.0	37.0	41.0	27.0	41.0
16	36.23780664148579	40.0	36.0	41.0	27.0	41.0
17	36.173554983299766	40.0	36.0	41.0	27.0	41.0
18	36.12838639650042	40.0	36.0	41.0	27.0	41.0
19	36.20900246588016	40.0	37.0	41.0	27.0	41.0
20	36.17281922585204	40.0	37.0	41.0	27.0	41.0
21	36.12141381985598	40.0	37.0	41.0	27.0	41.0
22	36.060622771328774	40.0	37.0	41.0	27.0	41.0
23	35.980636102379556	40.0	37.0	41.0	27.0	41.0
24	35.920213661048926	40.0	37.0	41.0	27.0	41.0
25	35.8675807785188	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5709693999698118
1101	2	0.6429871524182147
1101	3	0.5410079961094816
1101	4	0.7445017718017652
1101	5	0.6229435202283753
1101	6	0.6420833911794332
1101	7	0.746239930077607
1101	8	0.6270112489814039
1101	9	0.7512124957267403
1101	10	0.8825801481187128
1101	11	0.8158306325110871
1101	12	0.850779543948974
1101	13	0.8369228056388494
1101	14	0.9595449061557062
1101	15	0.9835065797524223
1101	16	1.0141207504369163
1101	17	0.9935620898949722
1101	18	1.013962263875186
1101	19	1.0458717078332214
1101	20	1.0300716685200086
1101	21	1.0152806505576564
1101	22	1.038020749381083
1101	23	0.9625884217529475
1101	24	1.066618779631355
1101	25	1.0121519272745019
1102	1	0.41353314706677224
1102	2	0.4534787638511659
1102	3	0.36235145001598923
1102	4	0.42500010709123615
1102	5	0.49569258033051256
1102	6	0.45086715283375156
1102	7	0.4911245075402988
1102	8	0.4862846105332608
1102	9	0.4479315543770497
1102	10	0.612544228891025
1102	11	0.6141545933440753
1102	12	0.5574275951548913
1102	13	0.4911293587959378
1102	14	0.6444892113107628
1102	15	0.5243301776291389
1102	16	0.5974003939429764
1102	17	0.7260646648069482
1102	18	0.6940353781768351
1102	19	0.636504148742226
1102	20	0.6325690520574838
1102	21	0.6662173588058309
1102	22	0.6957392404997691
1102	23	0.6669874272772205
1102	24	0.6396439309120154
1102	25	0.6988260184540067
1103	1	0.4701169536719867
1103	2	0.4740505282607188
1103	3	0.455112879007217
1103	4	0.5216236745317318
1103	5	0.5597827288789148
1103	6	0.4550305860589319
1103	7	0.5250366618819058
1103	8	0.5236109192524552
1103	9	0.5433128206067295
1103	10	0.49934734978675976
1103	11	0.5683465128388789
1103	12	0.5718417075527142
1103	13	0.5838485681370358
1103	14	0.6869119235634571
1103	15	0.662033224760421
1103	16	0.620065569328446
1103	17	0.7485007506135872
1103	18	0.6760618614119167
1103	19	0.6537262876712617
1103	20	0.6645079774954255
1103	21	0.5754016319861535
1103	22	0.5419942255969588
1103	23	0.6104902789027378
1103	24	0.6325546279560399
1103	25	0.6584393248403302
1104	1	0.35947957652229334
1104	2	0.41903788212265525
1104	3	0.3912007121843146
1104	4	0.5171395131563941
1104	5	0.5083124163512807
1104	6	0.4439371225499684
1104	7	0.3899956577372734
1104	8	0.478004936361593
1104	9	0.44303431078754585
1104	10	0.6131192004856842
1104	11	0.5551459492161257
1104	12	0.525804989535068
1104	13	0.6140805024360745
1104	14	0.613302202839705
1104	15	0.6681159287346716
1104	16	0.6583939798061635
1104	17	0.7241833178090928
1104	18	0.6227613667681027
1104	19	0.6864580303621466
1104	20	0.6793418679663077
1104	21	0.6335841844425758
1104	22	0.5985906752452834
1104	23	0.5903568606722516
1104	24	0.5822195789927846
1104	25	0.5889236512934559
1105	1	0.286951112977583
1105	2	0.38025645758263593
1105	3	0.22069941752541666
1105	4	0.33647980214591655
1105	5	0.3773168333815917
1105	6	0.37776389621397755
1105	7	0.3976007312046477
1105	8	0.4143065037874294
1105	9	0.4592983007804463
1105	10	0.44672523651561136
1105	11	0.42066220120474895
1105	12	0.42398184980458353
1105	13	0.45665836666265847
1105	14	0.5514802474781533
1105	15	0.510602220452796
1105	16	0.457890482851802
1105	17	0.5217489724652182
1105	18	0.4402060814721409
1105	19	0.44053363479764585
1105	20	0.5066447019310445
1105	21	0.46625101825242865
1105	22	0.46971825461303496
1105	23	0.5009104345901605
1105	24	0.5454712627034439
1105	25	0.5546091313801398
1106	1	0.17069543342342897
1106	2	0.21623391789737312
1106	3	0.20725099016352644
1106	4	0.27590190136040604
1106	5	0.2960813310237427
1106	6	0.33540436934716666
1106	7	0.2925820730140458
1106	8	0.2872870248314996
1106	9	0.3134718258951139
1106	10	0.3201532831542835
1106	11	0.2798256688197043
1106	12	0.3500737833470069
1106	13	0.2814723322644497
1106	14	0.32758791712542035
1106	15	0.37587517456498176
1106	16	0.32070966750256247
1106	17	0.21656910461112489
1106	18	0.31577562831028416
1106	19	0.29922090200827256
1106	20	0.3247370818894453
1106	21	0.3696247935796322
1106	22	0.40763104266127925
1106	23	0.21955676398383162
1106	24	0.3936771569478239
1106	25	0.39047066603843916
1107	1	0.44353504579241587
1107	2	0.45388546763090787
1107	3	0.4109358768402167
1107	4	0.5071794862335111
1107	5	0.5058173547261191
1107	6	0.37320892530924965
1107	7	0.4172112665201979
1107	8	0.3766885161909954
1107	9	0.5420515821909362
1107	10	0.5148277776534158
1107	11	0.54325061169083
1107	12	0.5610876386698749
1107	13	0.5711727877875461
1107	14	0.5914801378656378
1107	15	0.4217575089597858
1107	16	0.5387132709283335
1107	17	0.5604077080574541
1107	18	0.6625001481010244
1107	19	0.5805149824013114
1107	20	0.6518856804785003
1107	21	0.5566421225574132
1107	22	0.64853394619265
1107	23	0.6218098418148088
1107	24	0.5854542544948345
1107	25	0.5835313069510732
1108	1	0.19824998301264074
1108	2	0.3238183414085931
1108	3	0.2233723103418157
1108	4	0.3440102659276647
1108	5	0.19668315213320398
1108	6	0.138544113541748
1108	7	0.20592618504603166
1108	8	0.2701806643235827
1108	9	0.2453455897924357
1108	10	0.26234125653298435
1108	11	0.2592776148506317
1108	12	0.23983168038863312
1108	13	0.23537504019478206
1108	14	0.2877879508309107
1108	15	0.40741234459824227
1108	16	0.36015556111604496
1108	17	0.33092536023760033
1108	18	0.319300589466728
1108	19	0.3364559045600828
1108	20	0.3255282672839144
1108	21	0.3833937871372797
1108	22	0.3249475872238108
1108	23	0.342880603206261
1108	24	0.3023583918754795
1108	25	0.23749231489930622
1109	1	0.2507184106317375
1109	2	0.28446202717722
1109	3	0.23017028137708095
1109	4	0.3242872367061054
1109	5	0.31607255321382866
1109	6	0.28176280054554326
1109	7	0.35033981094363753
1109	8	0.3953558129773853
1109	9	0.2913426975028486
1109	10	0.41014913871201486
1109	11	0.42652093305189
1109	12	0.4323069203333887
1109	13	0.4816170221920828
1109	14	0.4749867541227246
1109	15	0.5318709305098821
1109	16	0.38724824257294443
1109	17	0.45312982525972245
1109	18	0.49733001577588
1109	19	0.5162427706810817
1109	20	0.4040123345427773
1109	21	0.36760620573321745
1109	22	0.44697439987884735
1109	23	0.40158299871462333
1109	24	0.4169759936252717
1109	25	0.4606579610153503
1110	1	0.20764398116085303
1110	2	0.16695172555854043
1110	3	0.19967753422873358
1110	4	0.1677900431080701
1110	5	0.23243034054886635
1110	6	0.17242933977556874
1110	7	0.21720524050370216
1110	8	0.1937997983948705
1110	9	0.26577075569518627
1110	10	0.3119550895026393
1110	11	0.2748921206583361
1110	12	0.21757677550591836
1110	13	0.22154408311180163
1110	14	0.31312055641111414
1110	15	0.26682685891815083
1110	16	0.2642818575912784
1110	17	0.34959381175438153
1110	18	0.34678434121961743
1110	19	0.35591673890786524
1110	20	0.2603708635413824
1110	21	0.345536968790249
1110	22	0.31794756401870927
1110	23	0.2616880324029225
1110	24	0.2903366081111969
1110	25	0.3461596743464881
1111	1	0.04795143837521465
1111	2	0.03509944973259138
1111	3	0.06420494160414236
1111	4	0.20811035153722912
1111	5	0.04543900971918191
1111	6	0.011161983211053439
1111	7	-0.0014782119373535352
1111	8	0.06438774147154902
1111	9	0.05269108086331897
1111	10	0.07664891470992075
1111	11	0.0275034281074511
1111	12	0.08397338667298015
1111	13	0.01717861184209113
1111	14	-0.003766209807473331
1111	15	0.07291860471248413
1111	16	0.014904142569669432
1111	17	0.05104551305543481
1111	18	0.0028643427315273584
1111	19	0.015516785345027984
1111	20	-0.058599311380930885
1111	21	-0.08032232120713445
1111	22	-0.02750810064017628
1111	23	-0.05555370812673033
1111	24	-0.009577051164221473
1111	25	-0.03595796826554931
1112	1	-0.34765969020323695
1112	2	-0.1924442387790002
1112	3	-0.08930854721541337
1112	4	-0.9887693360929362
1112	5	-0.3459958653087014
1112	6	-0.19681279932052576
1112	7	-0.20022158442441196
1112	8	-0.08160559943992496
1112	9	-0.10040501908213173
1112	10	-0.06020271768044694
1112	11	-0.04465163889996404
1112	12	-0.11489537446245635
1112	13	-0.09920242346074559
1112	14	-0.11640224681674027
1112	15	-0.0803294879880454
1112	16	-0.05927869482549397
1112	17	-0.07515415748360965
1112	18	-0.0710314927852238
1112	19	-0.015352416504903488
1112	20	-0.03729447400189656
1112	21	-0.11661059148335795
1112	22	-0.15193077808822864
1112	23	-0.17360118167714234
1112	24	-0.17274455223241603
1112	25	-0.12581212938969344
1113	1	-0.5151808289303155
1113	2	-0.364618580822583
1113	3	-0.43457118987797827
1113	4	-1.29322746243583
1113	5	-0.6517196965764924
1113	6	-0.6434184793774804
1113	7	-0.38382218679003444
1113	8	-0.34381504499327775
1113	9	-0.39006167196991015
1113	10	-0.3191614191674148
1113	11	-0.32827978610839637
1113	12	-0.26579649817565354
1113	13	-0.3784935511116956
1113	14	-0.26951536365795903
1113	15	-0.27242825629389245
1113	16	-0.29402412163801017
1113	17	-0.36065774639558157
1113	18	-0.38680628201221623
1113	19	-0.33034191907478316
1113	20	-0.25824064581834705
1113	21	-0.2727416790714301
1113	22	-0.28253148196888134
1113	23	-0.2918545541422546
1113	24	-0.40467503836009655
1113	25	-0.26595815585213956
1114	1	-0.49946163896756346
1114	2	-0.4861790269760107
1114	3	-0.29866588490901336
1114	4	-1.1522500700880727
1114	5	-0.5498828367233912
1114	6	-0.34651602010480076
1114	7	-0.30199335602429755
1114	8	-0.28479776469758633
1114	9	-0.3088071175557161
1114	10	-0.18880163867986965
1114	11	-0.28929431774179193
1114	12	-0.23753835984576455
1114	13	-0.2599487692891458
1114	14	-0.28779073344943384
1114	15	-0.24243587314533244
1114	16	-0.388016131871602
1114	17	-0.2743418861575151
1114	18	-0.3737599663549247
1114	19	-0.3858615361139499
1114	20	-0.3733939944107547
1114	21	-0.3678296026517742
1114	22	-0.29508628556383343
1114	23	-0.3471112915042127
1114	24	-0.2851104482843283
1114	25	-0.2952443471537123
1115	1	-0.1802833227903733
1115	2	-0.1322351027160238
1115	3	-0.10025891427237354
1115	4	0.0563019360586452
1115	5	-0.16187496536019097
1115	6	-0.12110315260854065
1115	7	-0.28883856287875886
1115	8	-0.24550278129344605
1115	9	-0.33200125035554606
1115	10	-0.21372985988726612
1115	11	-0.20295993380982225
1115	12	-0.28064515778527266
1115	13	-0.22150546027595652
1115	14	-0.246207187437264
1115	15	-0.2642186583694368
1115	16	-0.28005079324762505
1115	17	-0.24778779617712843
1115	18	-0.3401126446545959
1115	19	-0.291492803350728
1115	20	-0.3462898216049908
1115	21	-0.31958721772522836
1115	22	-0.37208904789019925
1115	23	-0.40046977984551546
1115	24	-0.3516399324310058
1115	25	-0.3916567350310558
1116	1	0.003518622904625346
1116	2	0.03982497927765394
1116	3	0.03544915645636948
1116	4	0.2249240553760714
1116	5	0.028401965569621268
1116	6	-0.0287557764724653
1116	7	-0.06814865535332615
1116	8	-0.05541124264690467
1116	9	-0.18206863146360774
1116	10	-0.04462943491464699
1116	11	-0.05963501215909872
1116	12	-0.21171756641211203
1116	13	-0.18016569326803733
1116	14	-0.33450185976042235
1116	15	-0.2851163174160618
1116	16	-0.25078565828682287
1116	17	-0.28616114874626675
1116	18	-0.31929690205944183
1116	19	-0.3427413810751929
1116	20	-0.2921940725815517
1116	21	-0.2610328141689351
1116	22	-0.1927924610572873
1116	23	-0.399628228511574
1116	24	-0.3955389219993961
1116	25	-0.41936899701365604
1201	1	0.26726712081937976
1201	2	0.29695104840891773
1201	3	0.2522795798676931
1201	4	0.29946282684705494
1201	5	0.3612463848811345
1201	6	0.2332299802250688
1201	7	0.2734255299863193
1201	8	0.28868556232941955
1201	9	0.42298456002575335
1201	10	0.37684243191224454
1201	11	0.32366179988586197
1201	12	0.3866866889070337
1201	13	0.3650912516672804
1201	14	0.41596201950726197
1201	15	0.5161092074301905
1201	16	0.4365950584729177
1201	17	0.4394476375688541
1201	18	0.5326637609786644
1201	19	0.3416660904735451
1201	20	0.35356121122419637
1201	21	0.4778738444866093
1201	22	0.417403163456747
1201	23	0.384986000653619
1201	24	0.2727962373983601
1201	25	0.41149178619126303
1202	1	0.2891480082421083
1202	2	0.3184792110447425
1202	3	0.35519005428494665
1202	4	0.34057457867848484
1202	5	0.39014301901511317
1202	6	0.22118247319381368
1202	7	0.3750967598236983
1202	8	0.3439011149575393
1202	9	0.4315640895063382
1202	10	0.3831576783023394
1202	11	0.3355646921687594
1202	12	0.2995238310926638
1202	13	0.3793486603665457
1202	14	0.44514909027276417
1202	15	0.4441675347348948
1202	16	0.4307275987347836
1202	17	0.5391721814801258
1202	18	0.4632908107689886
1202	19	0.45365500318188623
1202	20	0.46003907074243955
1202	21	0.4689702596704848
1202	22	0.42261019974888825
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2210	12	-0.18537523491643526
2210	13	-0.10109305920839518
2210	14	-0.07609908638315943
2210	15	-0.11713006360270839
2210	16	-0.10988724518607995
2210	17	-0.00933469831311129
2210	18	-0.06731799282259487
2210	19	-0.10603136058642093
2210	20	-0.05917802857444343
2210	21	-0.09405628875109073
2210	22	-0.08744331455500998
2210	23	-0.058758519307318124
2210	24	-0.0883119899744429
2210	25	-0.08732771620882573
2211	1	-0.7551052283271993
2211	2	-0.6637955281219234
2211	3	-0.47607088496400607
2211	4	-1.3152288979828626
2211	5	-0.818725574664235
2211	6	-0.6931132213531654
2211	7	-0.7678725195290212
2211	8	-0.6745190866301627
2211	9	-0.7754936864750448
2211	10	-0.6990686418870737
2211	11	-0.7656959777715571
2211	12	-0.7915704377932258
2211	13	-0.8496009092148853
2211	14	-0.81540695964393
2211	15	-0.7624402808280664
2211	16	-0.7678230229155503
2211	17	-0.799965243322788
2211	18	-0.9244739094374452
2211	19	-0.9720748798023777
2211	20	-0.9229163403470082
2211	21	-0.904518273891938
2211	22	-0.9644329883041252
2211	23	-0.9647547548091993
2211	24	-1.009933041169262
2211	25	-0.9620810606974786
2212	1	-0.16826382503076331
2212	2	-0.41309806655931425
2212	3	-0.363758514382571
2212	4	-0.11711141557498905
2212	5	-0.1236401694115088
2212	6	-0.2242789722501115
2212	7	-0.24017762737415893
2212	8	-0.4318511290432028
2212	9	-0.38192279014106845
2212	10	-0.3177977740927602
2212	11	-0.43900218445654104
2212	12	-0.4589852577984814
2212	13	-0.40278913729581234
2212	14	-0.42843161690606024
2212	15	-0.5561080590468137
2212	16	-0.49682992230548706
2212	17	-0.4730121937907583
2212	18	-0.4722165520927959
2212	19	-0.49690669067116744
2212	20	-0.5190018595907517
2212	21	-0.5362455772123838
2212	22	-0.532746032354531
2212	23	-0.5211678389660008
2212	24	-0.48502364726199687
2212	25	-0.5766581645127005
2213	1	-0.17600758766192115
2213	2	-0.2875715308802249
2213	3	-0.37649407113308087
2213	4	-0.19552180356021864
2213	5	-0.3425775128650983
2213	6	-0.3617317709451626
2213	7	-0.39962338560352606
2213	8	-0.443910125427017
2213	9	-0.6194348590824603
2213	10	-0.4867556072605126
2213	11	-0.4833068435176955
2213	12	-0.5139968419832215
2213	13	-0.6020029319708868
2213	14	-0.5854202586079253
2213	15	-0.5841582278620692
2213	16	-0.6546106663719655
2213	17	-0.600420035054448
2213	18	-0.5543838541071864
2213	19	-0.5945653160412334
2213	20	-0.6289476667955327
2213	21	-0.5829784673015652
2213	22	-0.6396094869003477
2213	23	-0.516809526838685
2213	24	-0.523951955251384
2213	25	-0.5119222119608793
2214	1	-0.44957768134002407
2214	2	-0.4329533888946884
2214	3	-0.5350972623133643
2214	4	-0.2929150317248954
2214	5	-0.475467125039323
2214	6	-0.5397091719863809
2214	7	-0.5871433108494983
2214	8	-0.6136475047958498
2214	9	-0.6273429241938473
2214	10	-0.7675832018612638
2214	11	-0.6320850235084308
2214	12	-0.7218055753264991
2214	13	-0.7258209624535681
2214	14	-0.8471989254421075
2214	15	-0.7951901396648822
2214	16	-0.8328387525275502
2214	17	-0.9237653562533339
2214	18	-0.8675044813500037
2214	19	-0.8221797438823941
2214	20	-0.8596349012891764
2214	21	-0.986715579691726
2214	22	-0.988864302331713
2214	23	-0.9628994672396232
2214	24	-1.011677414808041
2214	25	-0.9866434548250922
2215	1	-0.6854289575323627
2215	2	-0.7255286079114711
2215	3	-0.7598716135166335
2215	4	-0.596696660280287
2215	5	-0.8587690169799771
2215	6	-0.8634501617163721
2215	7	-0.988179391158468
2215	8	-1.0240584218532973
2215	9	-1.1826173877432922
2215	10	-1.1691520283127375
2215	11	-1.0768738214393139
2215	12	-1.0857797498831303
2215	13	-1.1795652652790451
2215	14	-1.295279942084683
2215	15	-1.3771971666899177
2215	16	-1.2730873861669707
2215	17	-1.3592404426880336
2215	18	-1.3404267228435884
2215	19	-1.3151422804628723
2215	20	-1.2879181818285446
2215	21	-1.2856249575968732
2215	22	-1.3462631184341376
2215	23	-1.3112083902835607
2215	24	-1.2965015245884146
2215	25	-1.428805469078391
2216	1	-0.4197411257532977
2216	2	-0.39833750519737166
2216	3	-0.3801781209676669
2216	4	-0.19603149614974313
2216	5	-0.46500740450130706
2216	6	-0.5309059641407572
2216	7	-0.7697600023566267
2216	8	-0.7161381450764992
2216	9	-0.795342827329776
2216	10	-0.7100440244565434
2216	11	-0.7069623214029122
2216	12	-0.774319654884799
2216	13	-0.8431201088617897
2216	14	-1.0679141295374492
2216	15	-0.9295723616353015
2216	16	-0.9882273647852458
2216	17	-1.060862239514769
2216	18	-1.0759164862809314
2216	19	-1.0975389405319973
2216	20	-0.9554453017490019
2216	21	-1.039025285197539
2216	22	-0.9411948427509884
2216	23	-1.1213906082892464
2216	24	-1.075939004457112
2216	25	-1.1338321579156911
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68770.0
3	0.0
4	1201.0
5	9370.0
6	10516.0
7	8106.0
8	6359.0
9	5569.0
10	4965.0
11	4547.0
12	4284.0
13	3924.0
14	3809.0
15	3847.0
16	3928.0
17	4304.0
18	4697.0
19	5564.0
20	7230.0
21	9113.0
22	11711.0
23	14236.0
24	16468.0
25	18412.0
26	20348.0
27	22617.0
28	25579.0
29	30496.0
30	38784.0
31	49726.0
32	64076.0
33	83531.0
34	110666.0
35	149005.0
36	213883.0
37	349989.0
38	1355840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40484105327611	19.558558762376492	13.295417806674603	27.7411823776728
2	13.52751221662262	20.69953938369575	40.12013543467135	25.65281296501028
3	16.613922552115774	25.13348122083684	30.188574779716827	28.064021447330557
4	11.111147535490094	17.485632403710333	37.98570998763757	33.41751007316201
5	12.421328451459585	38.46458654809021	36.31990854506529	12.794176455384914
6	31.557826371052972	37.94250326345471	18.092402911308582	12.407267454183726
7	27.548993460737464	31.510880067855595	23.010057377037235	17.930069094369706
8	24.84112398067077	37.31241336726088	20.712187143109546	17.134275508958805
9	26.285479496364356	14.653420842321363	20.078329723551153	38.98276993776313
10	14.1854250793889	29.28028015412265	35.28211016024938	21.25218460623907
11	34.112301354595026	22.17576589800653	24.966253501222013	18.74567924617643
12	22.767504288883142	24.83458205698842	32.28124874793751	20.116664906190927
13	28.806142481979407	21.841469766560916	25.89512906715426	23.45725868430542
14	20.69805035153117	21.9193944237271	27.810264229345037	29.572290995396695
15	22.901725387638546	30.975060736413074	23.213438864748113	22.909775011200267
16	22.151726298229445	27.97255114789089	27.694420263197195	22.18130229068247
17	21.666344251371722	28.63474063055688	27.83850236091788	21.860412757153522
18	21.610949281421526	27.625439262858176	29.441769548449624	21.321841907270677
19	23.285193427719115	27.02925182209239	27.541586686432563	22.143968063755935
20	23.543910514411014	27.398478220486837	27.766284097076277	21.291327168025877
21	22.824107395222928	26.801017538849763	27.182703879294017	23.192171186633292
22	23.132995904523384	27.94488954160092	26.960649331917182	21.961465221958516
23	22.116995762934124	27.741525868248818	27.514118696126026	22.627359672691032
24	22.86730505159408	27.14030748833533	28.010431729357816	21.981955730712773
25	22.590574721686792	27.297713905243114	27.615947445025768	22.495763928044326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	171.0
2	291.5
3	412.0
4	412.0
5	412.0
6	945.5
7	1479.0
8	1479.0
9	1479.0
10	2338.0
11	3197.0
12	3197.0
13	3197.0
14	6618.5
15	10040.0
16	10040.0
17	10040.0
18	19677.5
19	29315.0
20	29315.0
21	29315.0
22	47851.0
23	66387.0
24	66387.0
25	66387.0
26	97630.0
27	128873.0
28	128873.0
29	128873.0
30	167818.5
31	206764.0
32	206764.0
33	206764.0
34	238443.0
35	270122.0
36	270122.0
37	270122.0
38	293900.0
39	317678.0
40	317678.0
41	317678.0
42	332957.0
43	348236.0
44	348236.0
45	348236.0
46	358811.0
47	369386.0
48	369386.0
49	369386.0
50	353488.5
51	337591.0
52	337591.0
53	337591.0
54	296388.5
55	255186.0
56	255186.0
57	255186.0
58	218116.0
59	181046.0
60	181046.0
61	181046.0
62	147034.5
63	113023.0
64	113023.0
65	113023.0
66	87176.0
67	61329.0
68	61329.0
69	61329.0
70	44769.5
71	28210.0
72	28210.0
73	28210.0
74	19536.0
75	10862.0
76	10862.0
77	10862.0
78	7496.0
79	4130.0
80	4130.0
81	4130.0
82	2796.5
83	1463.0
84	1463.0
85	1463.0
86	961.0
87	459.0
88	459.0
89	459.0
90	279.5
91	100.0
92	100.0
93	100.0
94	54.5
95	9.0
96	9.0
97	9.0
98	5.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1818267910412425
2	0.008559554466084132
3	0.005099309043624588
4	0.0020397236174498354
5	0.08235384105453711
6	0.3316736296517536
7	0.029794534269177955
8	0.014751572590485418
9	0.005536392675935268
10	0.00451653086721035
11	0.0
12	0.0
13	0.0
14	0.0018940290733462759
15	0.0
16	0.0
17	0.020506507082576026
18	0.029685263361100285
19	0.0
20	0.0
21	8.013199925695783E-4
22	7.284727205177984E-5
23	0.006337712668504847
24	0.0
25	7.284727205177984E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2745470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.41778041633151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78517997857965	56.773456650631935
2	12.019977212851469	16.68800277542063
3	3.2124934961685323	6.690125043177608
4	1.2194044539715367	3.38593402497971
5	0.5727803621227329	1.9880570702311358
6	0.32649838926934033	1.3598876095550998
7	0.1923415238245236	0.9346345166055361
8	0.12791242751330797	0.7103517444510986
9	0.08786228365554423	0.5489284242310202
>10	0.41551711416792014	4.945535442924856
>50	0.02229751550219228	1.0672876635006772
>100	0.014429799414659219	1.9530183091254816
>500	0.0020986939420972765	1.0882794639571747
>1k	0.0012067490165663876	1.8665012612081575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4374	0.1593169839772425	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3980	0.14496607138304188	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3977	0.14485680047496421	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3949	0.14383693866623928	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3660	0.1333105078547571	No Hit
CCCATGTACTCTGCGTTGATACCAC	3624	0.13199925695782508	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2950	0.10744972627637527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.642363602588992E-5	0.0
3	0.0	0.0	0.0	3.642363602588992E-5	0.0
4	0.0	0.0	0.0	7.284727205177984E-5	0.0
5	0.0	3.642363602588992E-5	0.0	7.284727205177984E-5	0.0
6	3.642363602588992E-5	3.642363602588992E-5	0.0	7.284727205177984E-5	0.0
7	3.642363602588992E-5	3.642363602588992E-5	0.0	7.284727205177984E-5	0.0
8	7.284727205177984E-5	3.642363602588992E-5	0.0	1.0927090807766976E-4	0.0
9	7.284727205177984E-5	3.642363602588992E-5	0.0	1.0927090807766976E-4	0.0
10	7.284727205177984E-5	3.642363602588992E-5	0.0	1.821181801294496E-4	0.0
11	7.284727205177984E-5	3.642363602588992E-5	0.0	1.821181801294496E-4	0.0
12	7.284727205177984E-5	3.642363602588992E-5	0.0	1.821181801294496E-4	0.0
13	7.284727205177984E-5	3.642363602588992E-5	0.0	1.821181801294496E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	840	0.0	12.01362	4
GGCGAGG	965	0.0	10.914829	19
GTCTTAC	220	0.0	10.389311	1
ATTTAGA	855	0.0	10.3589325	1
GCGCCAC	140	9.831092E-8	10.169027	13
TGGCGAG	2295	0.0	10.090563	18
AATCCCG	170	1.5916157E-9	10.047195	19
AATGTCC	805	0.0	10.020372	8
CGGGACT	105	4.012435E-5	9.973572	4
TTTAGAA	990	0.0	9.905971	2
AAATGTC	805	0.0	9.904109	7
GTATTAA	350	0.0	9.795635	1
CACCTTT	905	0.0	9.753277	14
AGGACCT	1750	0.0	9.629031	5
AGGACGT	2410	0.0	9.599234	5
TAGAAAT	875	0.0	9.57463	4
GGACGTG	2380	0.0	9.559705	6
GACGTGG	1175	0.0	9.531827	7
GTCTAGG	260	0.0	9.523535	1
GTCCTAA	1340	0.0	9.523534	1
>>END_MODULE