FastQCFastQC Report
Thu 26 May 2016
SRR1513140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464782
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33980.13786209084616813No Hit
GTCCTACAGTGGACATTTCTAAATT30410.12337805128404865No Hit
GTCCTAAAGTGTGTATTTCTCATTT27390.11112544638836212No Hit
CCCATGTACTCTGCGTTGATACCAC26640.10808258093413536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7100.012.6170134
TATACGC600.00573980611.1186363
TATACCG600.005831924711.0948825
TTAGGAC10750.010.9930443
GTCCTAG3600.010.8796411
AATACCG700.001479031510.8684565
CGCAGAT1408.529241E-910.8430838
GTAGGAC15500.010.7599713
CGTTTAT803.8142162E-410.67474514
AGGACGT15250.010.6635585
GTCCTAC16200.010.4963471
GGACGTG14750.010.4836466
TGTAGGA17050.010.459582
TAGGACG15900.010.36897854
GGCGAGG7700.010.35210819
GACAGCG1106.141445E-610.3504277
GCACCGT1106.150376E-610.3491666
GACGTGA8450.010.2177297
TGGCGAG17300.010.14745218
GTATTAC2450.010.1377241
Produced by FastQC (version 0.11.5)