##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513140_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2464782
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.582565516950382	31.0	30.0	34.0	25.0	34.0
2	29.42833646139902	31.0	30.0	34.0	23.0	34.0
3	29.619946510482468	31.0	30.0	34.0	25.0	34.0
4	32.70494591408084	37.0	33.0	37.0	24.0	37.0
5	32.8610299004131	37.0	33.0	37.0	26.0	37.0
6	33.25142588675185	37.0	35.0	37.0	27.0	37.0
7	33.21188770447041	37.0	35.0	37.0	27.0	37.0
8	33.089695153567334	37.0	35.0	37.0	26.0	37.0
9	34.350192430811326	39.0	35.0	39.0	25.0	39.0
10	33.92037916537852	39.0	34.0	39.0	22.0	39.0
11	34.39091286775058	39.0	35.0	39.0	27.0	39.0
12	34.38909850850907	39.0	35.0	39.0	27.0	39.0
13	34.427230481235256	39.0	35.0	39.0	27.0	39.0
14	34.646479080097144	39.0	34.0	41.0	17.0	41.0
15	35.093699158789704	40.0	34.0	41.0	25.0	41.0
16	34.9450572099277	40.0	34.0	41.0	22.0	41.0
17	34.54855236690304	39.0	34.0	41.0	17.0	41.0
18	34.27553430688799	39.0	33.0	41.0	17.0	41.0
19	34.920881441036165	39.0	34.0	41.0	23.0	41.0
20	35.14425697688477	40.0	35.0	41.0	25.0	41.0
21	35.19199750728462	40.0	36.0	41.0	25.0	41.0
22	34.87730639058545	40.0	35.0	41.0	19.0	41.0
23	34.41048417263677	39.0	34.0	41.0	17.0	41.0
24	34.668853878355165	40.0	34.0	41.0	18.0	41.0
25	34.31161619972882	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8583323384611354
1101	2	1.1042434502578828
1101	3	1.0139655367409581
1101	4	1.306612090150054
1101	5	1.1766053023260952
1101	6	0.8926607321475757
1101	7	0.870915751638492
1101	8	0.8484958076086571
1101	9	1.2804493805896158
1101	10	1.605082474933269
1101	11	1.1984561610418822
1101	12	1.030832454318606
1101	13	0.9271828537549638
1101	14	1.9918666655713793
1101	15	1.6132332113041556
1101	16	1.6945878981787104
1101	17	2.017724670876923
1101	18	2.1115225757069283
1101	19	1.6340205207953034
1101	20	1.2563793755344008
1101	21	1.319028413184597
1101	22	1.619238819965453
1101	23	1.7565835200749902
1101	24	1.4798880646389065
1101	25	1.741391812541515
1102	1	0.8832556270797767
1102	2	1.1135957755627288
1102	3	1.0116134342747642
1102	4	1.3181430156862532
1102	5	1.3837027883881845
1102	6	1.0265024855128715
1102	7	1.0572956912411158
1102	8	1.0899659308837144
1102	9	1.508521442351011
1102	10	1.7020318347745018
1102	11	1.2872599133688638
1102	12	1.1656592864917101
1102	13	1.1186140027596423
1102	14	2.26563984581486
1102	15	1.7922510776855418
1102	16	1.9687496214562898
1102	17	2.3403374555772913
1102	18	2.440187544241091
1102	19	1.8756320551399028
1102	20	1.686976232219756
1102	21	1.6130371725731365
1102	22	1.874805512455012
1102	23	2.1726046894470628
1102	24	2.007145160760345
1102	25	2.2342096897799095
1103	1	0.8154446228036356
1103	2	1.0702742462366928
1103	3	0.967753931196615
1103	4	1.366536423002998
1103	5	1.1337162414483046
1103	6	0.9168320988644894
1103	7	0.851600929134328
1103	8	0.8790950853150363
1103	9	1.2474934263904771
1103	10	1.349732907481382
1103	11	1.0742098207137047
1103	12	0.9456874829164974
1103	13	1.0206051151181157
1103	14	1.7828007476842274
1103	15	1.3923027493286
1103	16	1.4731857122854066
1103	17	1.86444563661356
1103	18	2.200230736739961
1103	19	1.4664784146526415
1103	20	1.1748206119771964
1103	21	1.2305298075801687
1103	22	1.4042732142590921
1103	23	1.8433421579587232
1103	24	1.5325023209248982
1103	25	1.659823632442226
1104	1	0.7639065595816241
1104	2	0.9227115708516109
1104	3	0.8248527984459137
1104	4	1.2417787504141558
1104	5	1.1982723950945697
1104	6	0.9263706835454713
1104	7	0.8880636631288255
1104	8	0.9383304315373877
1104	9	1.1498113509079104
1104	10	1.3627150327024395
1104	11	0.9966435283818171
1104	12	0.9492925385881534
1104	13	0.9908367966832259
1104	14	2.004659581124777
1104	15	1.5559582846797682
1104	16	1.6674134211910783
1104	17	2.028168433460351
1104	18	2.1176982553466672
1104	19	1.5519051100545056
1104	20	1.2727994550999
1104	21	1.334181814985648
1104	22	1.6051780491642162
1104	23	1.929148348263233
1104	24	1.6973576204377139
1104	25	1.864351031434353
1105	1	0.5095110354329222
1105	2	0.6682600927255322
1105	3	0.6065672137224745
1105	4	1.0478102390073474
1105	5	0.8961005637128636
1105	6	0.6598914347381992
1105	7	0.599015191518653
1105	8	0.7133356950047514
1105	9	0.8895729037992979
1105	10	1.2223513070458907
1105	11	0.760927294856323
1105	12	0.827287918409148
1105	13	0.7261032098377669
1105	14	1.4145372746303337
1105	15	0.9954600710487966
1105	16	1.244279887571203
1105	17	1.5348321363413788
1105	18	1.1841719452320731
1105	19	1.0113885942933578
1105	20	0.9010046076222693
1105	21	0.8587279567364021
1105	22	0.9810287940086795
1105	23	1.450854406189535
1105	24	1.2110543078601168
1105	25	1.3606946177443504
1106	1	0.5713561456428415
1106	2	0.7896504481094375
1106	3	0.7493794912652909
1106	4	1.32147485168624
1106	5	1.166354032031741
1106	6	0.8020981455768776
1106	7	0.7075453071883331
1106	8	0.8065382087507871
1106	9	0.926377186573184
1106	10	0.9927932096849332
1106	11	0.7749374670885132
1106	12	0.7513263396838639
1106	13	0.6056872370496151
1106	14	1.5013120843438799
1106	15	1.203158176720187
1106	16	1.3922008289053167
1106	17	1.594419255808738
1106	18	1.740401068480999
1106	19	1.2963576556327538
1106	20	1.0740807254243236
1106	21	0.9628983142462388
1106	22	1.182650295491996
1106	23	1.4897153609878444
1106	24	1.1810980826021904
1106	25	1.5125742903517931
1107	1	0.43152743087360435
1107	2	0.6165749114335846
1107	3	0.6097957073516085
1107	4	0.9155190575464687
1107	5	0.7736596262524813
1107	6	0.5695589427759771
1107	7	0.4896150745872063
1107	8	0.5767163520455085
1107	9	0.8664153403306045
1107	10	1.0918922338434385
1107	11	0.7027531090199375
1107	12	0.6231369240241875
1107	13	0.5808299901463769
1107	14	1.3945046702406714
1107	15	0.9101330680725539
1107	16	1.0020583253607498
1107	17	1.468749393025476
1107	18	1.6739138945394245
1107	19	1.0341947590527312
1107	20	0.7822747254502005
1107	21	0.7010145464148039
1107	22	0.955913643299354
1107	23	1.2363773693469184
1107	24	0.8551191448072331
1107	25	1.1946771465228494
1108	1	0.5940968202471169
1108	2	0.7648699153986911
1108	3	0.5817756174758486
1108	4	1.0450458881042266
1108	5	0.9650421269133318
1108	6	0.5986363945338411
1108	7	0.581076877389215
1108	8	0.6290557652096638
1108	9	0.7807690278801687
1108	10	1.1019371717234279
1108	11	0.6496206744442077
1108	12	0.49202405064327337
1108	13	0.47985193040542384
1108	14	1.5372485243247311
1108	15	1.1654859626007052
1108	16	1.071051188168049
1108	17	1.5070787612517478
1108	18	1.7212308387635886
1108	19	1.185397508193624
1108	20	0.8615768666080115
1108	21	0.888696280488297
1108	22	1.1261489063181287
1108	23	1.421415698715144
1108	24	1.1662478786163746
1108	25	1.3857953067131774
1109	1	0.44976692333990087
1109	2	0.7225754401145252
1109	3	0.6917412532146798
1109	4	0.9038880768433231
1109	5	0.887326028578677
1109	6	0.46117934986030207
1109	7	0.4465275778914588
1109	8	0.5785667353391872
1109	9	0.6874568417844813
1109	10	1.131543304422344
1109	11	0.7701599290040804
1109	12	0.5134356287558788
1109	13	0.5313983221580045
1109	14	1.4917819633942528
1109	15	0.9976297638166756
1109	16	1.1561688196774327
1109	17	1.4708641167896843
1109	18	1.6466868260752463
1109	19	1.0323443757590525
1109	20	0.9567394359973846
1109	21	0.8416436767346624
1109	22	1.139630270314953
1109	23	1.4132211441288405
1109	24	1.1662478786163746
1109	25	1.3815658591847608
1110	1	0.2820096293193579
1110	2	0.4678737257698664
1110	3	0.47937756791619535
1110	4	0.7872032273383738
1110	5	0.56449957735704
1110	6	0.2761485652382518
1110	7	0.26420775774723637
1110	8	0.361675379272576
1110	9	0.45214601676099875
1110	10	0.7626078228391364
1110	11	0.4138980434567827
1110	12	0.3561568211659747
1110	13	0.16652410885211566
1110	14	1.0162331139749057
1110	15	0.7085555576714384
1110	16	0.9180934624782822
1110	17	1.1847005365414986
1110	18	0.8092531975502837
1110	19	0.6141666679050672
1110	20	0.4102362269424802
1110	21	0.31352680834205415
1110	22	0.6146159387266508
1110	23	-0.5732096984642965
1110	24	0.1292508439759814
1110	25	0.5254110404445456
1111	1	0.2491060721635847
1111	2	0.6135614300695877
1111	3	0.4740172246659107
1111	4	0.9473059991272237
1111	5	0.7510519075107425
1111	6	0.412230103230641
1111	7	0.4370311464878114
1111	8	0.43306713184989576
1111	9	0.6453407963179174
1111	10	0.8423350041315629
1111	11	0.6254335213910736
1111	12	0.4699450128425866
1111	13	0.25812314372818435
1111	14	1.2254060712451675
1111	15	0.7978478447048545
1111	16	0.8459190179327791
1111	17	1.16364101343256
1111	18	1.4403360462708577
1111	19	1.0254120469195058
1111	20	0.667478267612502
1111	21	0.34661988609231287
1111	22	0.6704589776900534
1111	23	1.0055750960188732
1111	24	0.6990128799380813
1111	25	0.9152626606650642
1112	1	0.11678778289878267
1112	2	0.26555060412265163
1112	3	0.13101045443351822
1112	4	0.5677038339378342
1112	5	0.3246670750800149
1112	6	0.09352877515144797
1112	7	-0.025703596395949546
1112	8	0.08031988719259431
1112	9	0.28870408125930425
1112	10	0.5139109370128381
1112	11	0.07932687349937595
1112	12	0.001279021682172754
1112	13	-0.03526376608503767
1112	14	0.5499229325772035
1112	15	0.41294440551246225
1112	16	0.28962643627639295
1112	17	0.7449159935555372
1112	18	0.8519713291134394
1112	19	0.37027500927765544
1112	20	0.18661501613411247
1112	21	0.19529374774827346
1112	22	0.5955698071171369
1112	23	0.8855517120805771
1112	24	0.46333959116852697
1112	25	0.8044806840998717
1113	1	0.004550459689998121
1113	2	0.015360217715528535
1113	3	0.015490192158598148
1113	4	0.2981522990994776
1113	5	0.38782166696091025
1113	6	0.029313516604958068
1113	7	-0.07098148931505222
1113	8	0.03714121778189394
1113	9	0.04360123899790125
1113	10	0.40117899892356945
1113	11	0.04703604855164656
1113	12	-0.03408264932043892
1113	13	-0.15655637218395668
1113	14	0.42165108202402024
1113	15	0.12893042565296753
1113	16	0.2878540886890093
1113	17	0.6280323818131706
1113	18	0.4343329726971845
1113	19	0.2901732046322536
1113	20	0.03313588431225867
1113	21	0.044734120580372405
1113	22	0.14683354813679017
1113	23	0.23606377288810165
1113	24	0.11512406219672755
1113	25	0.5704445137738574
1114	1	-0.8524344427518251
1114	2	-0.5058945576439058
1114	3	-0.5676418456445695
1114	4	-0.27662826910206917
1114	5	-3.6388335986416536
1114	6	-1.279125526387574
1114	7	-0.9580097824181308
1114	8	-0.5373618642034828
1114	9	-0.20357688617093572
1114	10	0.014364328243054558
1114	11	-0.4149743049888741
1114	12	-0.5881891174769294
1114	13	-0.6123868873798699
1114	14	-6.320332438232214
1114	15	-2.429687422847948
1114	16	-1.3507127054954253
1114	17	-0.3597842554000934
1114	18	-6.355361260048181
1114	19	-2.4884352832986707
1114	20	-1.39971468070528
1114	21	-1.1068479049221338
1114	22	-0.7991803020447819
1114	23	-0.3227399625274856
1114	24	-0.45317852734240915
1114	25	-0.2517901577354422
1115	1	-0.8470703791682617
1115	2	-0.45623293835491197
1115	3	-0.5042715962890689
1115	4	-3.1467554637125374
1115	5	-1.0648122688801571
1115	6	-0.7291018182637217
1115	7	-0.6503281522596609
1115	8	-0.4964096231700239
1115	9	-0.33774370249603436
1115	10	-0.5982772320299787
1115	11	-0.7166134268711559
1115	12	-0.7181586490412855
1115	13	-0.7573135778507876
1115	14	-6.117415961500768
1115	15	-2.51643619681429
1115	16	-1.3417694021337994
1115	17	-0.4951182218577088
1115	18	-0.30129931148175615
1115	19	-0.5914654303038915
1115	20	-0.5777406597592076
1115	21	-0.7623790764326799
1115	22	-1.893510044112034
1115	23	-1.074239342007104
1115	24	-0.7640225402644774
1115	25	-0.32577992617310514
1116	1	-0.38418778017116395
1116	2	-0.06997262466055076
1116	3	-0.17934229246568378
1116	4	0.03685837935766756
1116	5	-0.594791690882225
1116	6	-0.399949153136852
1116	7	-0.353330910061608
1116	8	-0.35431726778625006
1116	9	-0.3686553756044617
1116	10	-0.02359084107764886
1116	11	-0.42089664082845246
1116	12	-0.5909223689039251
1116	13	-0.5685108958412499
1116	14	0.2853609681523821
1116	15	-0.25648148367598367
1116	16	-0.20952556969608338
1116	17	0.28472481422091533
1116	18	0.18465552019444686
1116	19	-0.11339651129759432
1116	20	-0.5763361653771852
1116	21	-0.6089511986560225
1116	22	-0.6182552999024296
1116	23	-1.2902528678986513
1116	24	-1.0089791796434824
1116	25	-0.26611810040224526
1201	1	0.6256200004164292
1201	2	0.8008820339471399
1201	3	0.7604587734289083
1201	4	1.0040691341415098
1201	5	1.0630224701737632
1201	6	0.6905972738813801
1201	7	0.5545854311051954
1201	8	0.7061441635003973
1201	9	0.810323407173712
1201	10	1.3019648073180718
1201	11	0.7731001014884029
1201	12	0.7458839137131719
1201	13	0.6317311653178237
1201	14	1.5473920470132825
1201	15	1.157943428539724
1201	16	1.1803464182557235
1201	17	1.6222438408622395
1201	18	1.0984572847392542
1201	19	0.9351954165743166
1201	20	0.9259913264081376
1201	21	0.842941348135426
1201	22	1.1045735992503296
1201	23	1.393148350611753
1201	24	1.1874203053284873
1201	25	1.447265178703482
1202	1	0.8296953317197051
1202	2	0.9858231143790306
1202	3	0.9268612588274863
1202	4	1.233742250198226
1202	5	1.1151898528411621
1202	6	0.7827318859342682
1202	7	0.6304607089653587
1202	8	0.8182437438838335
1202	9	1.0767709668494092
1202	10	1.2456523530163395
1202	11	0.8388521665363129
1202	12	0.7896093394228316
1202	13	0.7435077378521768
1202	14	1.6496287295464853
1202	15	1.1703589693282765
1202	16	1.290650368641721
1202	17	1.7597073189520316
1202	18	1.8701051239635689
1202	19	1.3327439338214688
1202	20	1.0753394378399719
1202	21	0.9707990132585067
1202	22	1.1943313139991645
1202	23	1.5024456910825847
1202	24	1.1422441339111629
1202	25	1.5711172528543358
1203	1	0.7355939596624417
1203	2	0.7772081259167685
1203	3	0.709203077198044
1203	4	1.0684220013781314
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2211	14	0.11932317145475224
2211	15	-0.11241382371726161
2211	16	-6.449899021708468
2211	17	-1.8556825000205706
2211	18	-0.6042457310955456
2211	19	-0.5574028967465168
2211	20	-0.7544837401965836
2211	21	-0.644510570431045
2211	22	-0.5502768749798292
2211	23	-0.16550280339916412
2211	24	-0.5004934662791882
2211	25	-7.584697176769637
2212	1	-1.5779609902127554
2212	2	-3.9320831601626303
2212	3	-1.8730175561317566
2212	4	-3.7247522118485143
2212	5	-1.4106631815239368
2212	6	-1.1951061458581833
2212	7	-0.9857455899487135
2212	8	-0.8259508833536842
2212	9	-0.9337985351410012
2212	10	-0.3639442835910174
2212	11	-0.8524353737458625
2212	12	-0.9790917465034497
2212	13	-1.252076793791339
2212	14	-6.596930698514285
2212	15	-5.100009595678358
2212	16	-2.8450369333324446
2212	17	-7.722985561596079
2212	18	-3.2356792614454832
2212	19	-2.041806530608355
2212	20	-2.0414876805287037
2212	21	-1.6646344094403318
2212	22	-1.540035138731902
2212	23	-0.9340817820400673
2212	24	-1.4083780395662444
2212	25	-1.0933518348050981
2213	1	-0.20865204712332286
2213	2	-0.18376460879530043
2213	3	-0.20944894317290164
2213	4	0.23393835232985793
2213	5	0.049345441289567304
2213	6	-0.280219474264193
2213	7	-0.2982486695249662
2213	8	-0.3671987276503117
2213	9	0.04178658434655347
2213	10	-3.765601095613686
2213	11	-1.3903326390821533
2213	12	-0.8359222466266374
2213	13	-0.7043433619115618
2213	14	0.11797445053720423
2213	15	-0.35347103938370594
2213	16	-0.06971665740241662
2213	17	0.23636403189985344
2213	18	0.350844512883171
2213	19	-0.2493884473366137
2213	20	-0.4982073159068747
2213	21	-0.4726536259961378
2213	22	-0.8995448701928481
2213	23	-1.009235760852583
2213	24	-0.5598377587088876
2213	25	-0.2420953676949722
2214	1	-0.9895022513236178
2214	2	-0.6614077637985964
2214	3	-0.6703935541840949
2214	4	-0.32314775110730665
2214	5	-3.607484940679683
2214	6	-1.4086368041379806
2214	7	-1.1326516898061172
2214	8	-0.8633615120210791
2214	9	-0.7642044138990727
2214	10	-4.296490230020389
2214	11	-1.776057850356537
2214	12	-1.2161915939697536
2214	13	-0.9988273788350881
2214	14	-0.2725660485716688
2214	15	-0.5098005698569352
2214	16	-0.5654266395436025
2214	17	-0.3447814844249564
2214	18	0.056458048694778995
2214	19	-0.7016984900453167
2214	20	-0.9600671048754634
2214	21	-1.0991728583663871
2214	22	-0.7102331841144007
2214	23	-0.4608405154638291
2214	24	-0.9573258303118664
2214	25	-0.7221546191661687
2215	1	-2.0866780347193377
2215	2	-4.6608706108379465
2215	3	-2.756798878681547
2215	4	-1.678418384350966
2215	5	-1.575678373403985
2215	6	-1.8148401167122827
2215	7	-2.0242905209736684
2215	8	-2.221395582219312
2215	9	-7.878434774013133
2215	10	-3.6515183164847436
2215	11	-3.0056358820989537
2215	12	-3.322420590770779
2215	13	-2.7115412616718864
2215	14	-7.798561440421121
2215	15	-4.586786299505196
2215	16	-9.360481460953046
2215	17	-4.482545315101099
2215	18	-2.8578630758550183
2215	19	-3.471942658171219
2215	20	-3.246383392308644
2215	21	-3.426703514146883
2215	22	-9.678568082605118
2215	23	-10.756450418758423
2215	24	-6.733939233975825
2215	25	-5.039917882787954
2216	1	-1.698206585458351
2216	2	-1.7256650287445012
2216	3	-1.5507423088565098
2216	4	-6.899423561519864
2216	5	-3.438379534240287
2216	6	-2.4808634066154447
2216	7	-1.9470730012761273
2216	8	-8.19445767686048
2216	9	-3.8359825373250054
2216	10	-2.0403827850295215
2216	11	-2.0807544270649174
2216	12	-2.132305274716977
2216	13	-1.935622227336772
2216	14	-1.2185985201821055
2216	15	-1.9135042091729701
2216	16	-1.5222240855195963
2216	17	-1.3027961414066596
2216	18	-7.238184563950785
2216	19	-4.522068085869908
2216	20	-3.8412068052478254
2216	21	-3.020663515469437
2216	22	-2.6867885354852987
2216	23	-1.8677227656810516
2216	24	-2.268723174911379
2216	25	-1.6242901157433778
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103346.0
3	2.0
4	886.0
5	5140.0
6	6214.0
7	5658.0
8	5063.0
9	4969.0
10	4712.0
11	4432.0
12	4405.0
13	4437.0
14	4332.0
15	4483.0
16	4418.0
17	4837.0
18	5508.0
19	6815.0
20	8664.0
21	11326.0
22	14105.0
23	16845.0
24	19445.0
25	22577.0
26	25866.0
27	28935.0
28	32593.0
29	38928.0
30	46821.0
31	55866.0
32	71490.0
33	94491.0
34	124849.0
35	166040.0
36	237625.0
37	347949.0
38	920710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56291775646615	18.472878472878474	12.307570372086502	25.656633398568886
2	14.399395698132182	20.237644882349066	41.4431652791642	23.919794140354558
3	18.391368936240834	24.949682229244022	29.72568069327068	26.933268141244465
4	11.389737049233517	16.89262310417311	38.989356997312655	32.72828284928072
5	12.71111121957926	38.00154485761318	36.17492766869271	13.112416254114843
6	31.727723790791373	37.17232275430075	18.349925759157756	12.750027695750113
7	27.966658117882325	31.439141853549586	22.627428287602747	17.966771740965342
8	25.14067723911864	36.47061735669222	20.909058814792992	17.47964658939614
9	26.62269994416237	14.84623550494098	20.166920424885404	38.36414412601125
10	13.959614159392588	28.7129448144045	35.7402920993576	21.587148926845305
11	34.326858537907704	22.233589343817236	24.659101330967744	18.780450787307313
12	23.186740091127174	24.670935285258413	31.927099883970524	20.21522473964389
13	28.94155002970712	21.735585554873754	25.712362056706507	23.610502358712626
14	20.507569716265	21.608785375563848	28.731319206442933	29.15232570172822
15	23.307242833230237	30.293351711955687	23.38432102192933	23.015084432884745
16	22.217618539970807	27.745026704287906	27.82578277590677	22.211571979834517
17	21.716504662063322	28.067196056688353	28.205686044982457	22.01061323626587
18	21.607782004520402	27.224880790570875	29.71942104133175	21.44791616357697
19	23.472749977374676	26.932496987686548	27.449368986787213	22.145384048151563
20	23.79628164075341	27.102382720709095	27.612165633789097	21.489170004748395
21	23.337739700202	26.621166227450516	26.89102744921686	23.150066623130623
22	23.330088843283825	27.602505861188863	26.968450243214985	22.098955052312327
23	22.454541322307342	27.249719561233054	27.651668265695236	22.64407085076437
24	23.139472343603806	27.024174610874212	27.630823187005664	22.205529858516318
25	22.908834100101267	26.871444777488477	27.515367490527865	22.704353631882395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	209.0
1	209.0
2	354.5
3	500.0
4	500.0
5	500.0
6	988.0
7	1476.0
8	1476.0
9	1476.0
10	2389.5
11	3303.0
12	3303.0
13	3303.0
14	6435.5
15	9568.0
16	9568.0
17	9568.0
18	18153.0
19	26738.0
20	26738.0
21	26738.0
22	43268.5
23	59799.0
24	59799.0
25	59799.0
26	86035.0
27	112271.0
28	112271.0
29	112271.0
30	145258.5
31	178246.0
32	178246.0
33	178246.0
34	206020.0
35	233794.0
36	233794.0
37	233794.0
38	256175.0
39	278556.0
40	278556.0
41	278556.0
42	294071.0
43	309586.0
44	309586.0
45	309586.0
46	319707.5
47	329829.0
48	329829.0
49	329829.0
50	317607.5
51	305386.0
52	305386.0
53	305386.0
54	271105.5
55	236825.0
56	236825.0
57	236825.0
58	203081.0
59	169337.0
60	169337.0
61	169337.0
62	137883.5
63	106430.0
64	106430.0
65	106430.0
66	82200.0
67	57970.0
68	57970.0
69	57970.0
70	42611.5
71	27253.0
72	27253.0
73	27253.0
74	19194.5
75	11136.0
76	11136.0
77	11136.0
78	7742.5
79	4349.0
80	4349.0
81	4349.0
82	2918.0
83	1487.0
84	1487.0
85	1487.0
86	1003.0
87	519.0
88	519.0
89	519.0
90	345.0
91	171.0
92	171.0
93	171.0
94	100.5
95	30.0
96	30.0
97	30.0
98	22.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18123306645374723
2	0.2599824244091364
3	0.259901281330357
4	0.4638544098423309
5	0.2557224127732189
6	0.020488627391793676
7	0.02004234045850708
8	0.018378907343529774
9	0.02004234045850708
10	0.022395489743109127
11	0.022720062058226652
12	0.030550369160436908
13	0.029454937596915264
14	0.027872647560717334
15	0.04260011635917497
16	0.023166348991513246
17	0.04324926098941002
18	0.03412066462672966
19	0.029576652215084338
20	0.032132659196634834
21	0.037447530856684286
22	0.04535898103767392
23	0.031889229960296694
24	0.050592709618943986
25	0.039638393983727566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2464782.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.75775844395982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43304794561331	62.275923550598236
2	10.752991359228265	15.862330784478909
3	2.5104257070771108	5.554901186823017
4	0.9303652433629372	2.744866195384776
5	0.4266946738137287	1.573602134023861
6	0.2305503858295606	1.020292780030708
7	0.1436490634265008	0.7416663044639036
8	0.10981309601198262	0.6479654247708145
9	0.0766463555966063	0.5087937043533902
>10	0.35436835146205176	4.5120746956352065
>50	0.01846855505217541	0.9492012998574391
>100	0.010476475029124221	1.561577392647739
>500	0.001557290620131891	0.7543169056874598
>1k	9.454978764942874E-4	1.2924876412445754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3398	0.13786209084616813	No Hit
GTCCTACAGTGGACATTTCTAAATT	3041	0.12337805128404865	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2739	0.11112544638836212	No Hit
CCCATGTACTCTGCGTTGATACCAC	2664	0.10808258093413536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.0571539389690446E-5	0.0
10	0.0	0.0	0.0	4.0571539389690446E-5	0.0
11	0.0	0.0	0.0	4.0571539389690446E-5	0.0
12	0.0	0.0	0.0	4.0571539389690446E-5	8.114307877938089E-5
13	0.0	0.0	0.0	4.0571539389690446E-5	8.114307877938089E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	710	0.0	12.617013	4
TATACGC	60	0.005739806	11.118636	3
TATACCG	60	0.0058319247	11.094882	5
TTAGGAC	1075	0.0	10.993044	3
GTCCTAG	360	0.0	10.879641	1
AATACCG	70	0.0014790315	10.868456	5
CGCAGAT	140	8.529241E-9	10.843083	8
GTAGGAC	1550	0.0	10.759971	3
CGTTTAT	80	3.8142162E-4	10.674745	14
AGGACGT	1525	0.0	10.663558	5
GTCCTAC	1620	0.0	10.496347	1
GGACGTG	1475	0.0	10.483646	6
TGTAGGA	1705	0.0	10.45958	2
TAGGACG	1590	0.0	10.3689785	4
GGCGAGG	770	0.0	10.352108	19
GACAGCG	110	6.141445E-6	10.350427	7
GCACCGT	110	6.150376E-6	10.349166	6
GACGTGA	845	0.0	10.217729	7
TGGCGAG	1730	0.0	10.147452	18
GTATTAC	245	0.0	10.137724	1
>>END_MODULE