FastQCFastQC Report
Thu 26 May 2016
SRR1513140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464782
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC36160.14670668643312065No Hit
CCCATGTACTCTGCGTTGATACCAC35460.14386667867584232No Hit
GTATCAACGCAGAGTACTTTTTTTT30720.12463576900512904No Hit
CTTTAGGACGTGAAATATGGCGAGG30110.12216090510235793No Hit
GTCCTACAGTGGACATTTCTAAATT30010.12175518970846103No Hit
GTCCTAAAGTGTGTATTTCTCATTT29240.11863118117545488No Hit
CTGTAGGACGTGGAATATGGCAAGA26510.10755515092206937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG400.005204209614.2811945
CGAGCCG400.0053148514.23598314
CGTGCGC1001.0266376E-813.28287310
TAGGACC6300.012.9966424
GTACTAG905.2184987E-712.7042231
TATTCCG657.880439E-411.7179035
TGGACCG752.0330377E-411.4249555
AGTGTCG855.39591E-511.16207910
TGCACCG951.3283874E-511.0240795
TGTAGGA17900.010.8531682
GCGGCTT700.001506275810.84470211
ACCGTCC700.001506785710.8442628
GTCCTAA9050.010.7389281
GTAGGAC17300.010.6225473
GGCGAGG8150.010.59348819
GTCCTAC16300.010.4634481
GCGTGCG1002.4343048E-510.4365439
AGGACCT13400.010.3023535
AGGACGT16850.010.2270875
CCAACGA1502.4967449E-810.1200819
Produced by FastQC (version 0.11.5)