FastQCFastQC Report
Thu 26 May 2016
SRR1513139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCG180.7053291536050157No Hit
TCTCCGAGCCCACGAGACGTAGAGG100.3918495297805642No Hit
TCTCCGAGCCCACGAGACGGACTCC100.3918495297805642No Hit
TCTCCGAGCCCACGAGACAGGCAGA70.274294670846395No Hit
CTCCGAGCCCACGAGACTAAGGCGA50.1959247648902821No Hit
TCTCCGAGCCCACGAGACTCCTGAG40.1567398119122257No Hit
CTCCGAGCCCACGAGACAGGCAGAA30.11755485893416928No Hit
CTCCGAGCCCACGAGACTAGGCATG30.11755485893416928No Hit
CTCCGAGCCCACGAGACGGACTCCT30.11755485893416928No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)