FastQCFastQC Report
Thu 26 May 2016
SRR1513138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947765
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24920.12794151245145077No Hit
GTACATGGGAAGCAGTGGTATCAAC22290.11443885684361307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT400.005327892514.230516
ATACCGT957.523886E-812.9822186
TATACCG604.0541776E-412.6809295
CGTTAGG550.002945369812.1574161
ATTCCGT600.00593673411.0681736
GTCTTAT1658.440111E-1010.4206431
CTAAGAC1909.458745E-1110.0306263
CCTACAC2300.09.9434043
GGGTTAC1355.97749E-79.9060431
GTCCTAA3500.09.8251781
GTCCTAC5250.09.8251771
TATACTG1856.5683707E-99.2536515
TAGGACC3300.09.2400964
GTATAAT2700.09.1984691
TAGGACT2600.09.1623554
CGCAGAT1451.7376169E-69.1601038
CTTACAC2501.8189894E-129.1479313
TTAGTAC1054.3574683E-49.0753293
CTAGGAC2002.5120244E-99.0526413
TGTACTG3900.09.0229685
Produced by FastQC (version 0.11.5)