##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1947765
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.256591529265595	31.0	30.0	34.0	23.0	34.0
2	29.089888153858396	31.0	30.0	34.0	20.0	34.0
3	29.30661655795232	31.0	30.0	34.0	22.0	34.0
4	32.37371140769035	35.0	33.0	37.0	19.0	37.0
5	32.534290841040885	37.0	33.0	37.0	22.0	37.0
6	32.87338667652412	37.0	35.0	37.0	26.0	37.0
7	32.83482555647114	37.0	35.0	37.0	26.0	37.0
8	32.71716865227582	37.0	35.0	37.0	25.0	37.0
9	33.935645213873336	39.0	34.0	39.0	22.0	39.0
10	33.52354416472213	38.0	34.0	39.0	17.0	39.0
11	33.95603679088597	39.0	34.0	39.0	25.0	39.0
12	33.9512975127903	39.0	35.0	39.0	24.0	39.0
13	33.98246040975169	39.0	35.0	39.0	24.0	39.0
14	34.21095614717381	39.0	33.0	41.0	16.0	41.0
15	34.627050491204024	39.0	34.0	41.0	17.0	41.0
16	34.452875988633124	39.0	34.0	41.0	17.0	41.0
17	34.05702535983551	39.0	33.0	41.0	16.0	41.0
18	33.78182172900735	39.0	33.0	41.0	11.0	41.0
19	34.424107631054056	39.0	34.0	41.0	18.0	41.0
20	34.640501292507054	39.0	34.0	41.0	18.0	41.0
21	34.687102396849724	40.0	35.0	41.0	18.0	41.0
22	34.37322572281564	40.0	34.0	41.0	13.0	41.0
23	33.8984718382351	39.0	34.0	41.0	10.0	41.0
24	34.145368666137855	39.0	34.0	41.0	2.0	41.0
25	33.78019422260899	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.893154580927952
1101	2	1.1695312564541496
1101	3	1.0104277021149102
1101	4	1.4547486824069011
1101	5	1.2881184956241043
1101	6	0.9943871078186746
1101	7	1.0028421413572275
1101	8	1.0969754166082808
1101	9	1.4221999010725384
1101	10	1.812677533529424
1101	11	1.4188605680078723
1101	12	1.2786580308016937
1101	13	1.2263706852215321
1101	14	2.2185709803383205
1101	15	1.7367000971831956
1101	16	1.9425265250904715
1101	17	2.1686388787687747
1101	18	2.185943370185946
1101	19	1.7269108810954776
1101	20	1.3830395418831571
1101	21	1.2930616361193117
1101	22	1.6085606414150035
1101	23	1.9083554191861367
1101	24	1.4966243612960994
1101	25	1.8471550312116278
1102	1	1.1518993198456506
1102	2	1.2416291980884182
1102	3	1.1807662494163758
1102	4	1.614475257357988
1102	5	1.5561522875353617
1102	6	1.2017650704856422
1102	7	1.1995913799689788
1102	8	1.2257113376146833
1102	9	1.6470370954411706
1102	10	1.8868861899728984
1102	11	1.4403069381228306
1102	12	1.3838231397924758
1102	13	1.2663535147240736
1102	14	2.3713514143470746
1102	15	1.8268975579039406
1102	16	1.9603266074530197
1102	17	2.27494752427711
1102	18	2.553353859079799
1102	19	1.9525672803630556
1102	20	1.7301860518283192
1102	21	1.5930393005128636
1102	22	1.7758734120306485
1102	23	2.177004138262376
1102	24	1.8759706725474317
1102	25	2.2649900129592453
1103	1	0.5626572121441775
1103	2	0.8528519255499916
1103	3	0.6353167575041638
1103	4	1.0153404027051423
1103	5	0.8943017064972096
1103	6	0.6717577058180808
1103	7	0.6446470838262499
1103	8	0.6545065146742459
1103	9	0.9311068065572741
1103	10	1.1917133315000825
1103	11	0.7364305187763875
1103	12	0.6178265735426152
1103	13	0.6629786958524235
1103	14	1.5997393180953026
1103	15	1.1218101346811196
1103	16	1.2154880835338702
1103	17	1.5781057202608224
1103	18	1.8846789491536526
1103	19	1.180452214221468
1103	20	0.795763507450566
1103	21	0.9331646228766459
1103	22	1.1015460675283109
1103	23	1.4213366749365832
1103	24	1.1327162600717315
1103	25	1.3502714938957112
1104	1	0.5520220743556123
1104	2	0.9190157925366229
1104	3	0.7220392896153136
1104	4	1.1264137662070652
1104	5	0.9824410517011657
1104	6	0.7410275310175365
1104	7	0.6807684944786843
1104	8	0.7242101258884688
1104	9	1.0553967408451541
1104	10	1.1601731634414705
1104	11	0.7865047120900215
1104	12	0.7789202360788323
1104	13	0.8073528882964212
1104	14	1.7097330796322225
1104	15	1.3343767244387834
1104	16	1.3573978999820824
1104	17	1.7656096393246585
1104	18	1.8356765768899592
1104	19	1.2163866540619566
1104	20	1.013201636589038
1104	21	0.941999050954216
1104	22	1.2525769472339903
1104	23	1.5791786015277722
1104	24	1.346246996745883
1104	25	1.5286733442888547
1105	1	0.7419434221623007
1105	2	0.9877385548564348
1105	3	0.9752522001582342
1105	4	1.209691525758636
1105	5	1.1610297118747255
1105	6	0.8097681963006025
1105	7	0.7936369430571233
1105	8	0.8219995798794173
1105	9	1.1019635790968039
1105	10	1.4558106141132114
1105	11	0.8886495094665179
1105	12	0.8629159044568766
1105	13	0.7842820109124773
1105	14	1.64208359995947
1105	15	1.221624980802055
1105	16	1.4866435317709374
1105	17	1.8278639491273836
1105	18	1.3234584758304138
1105	19	1.2794967255082028
1105	20	1.1547867739070057
1105	21	1.0080738531152775
1105	22	1.1842902339629262
1105	23	1.4642551165548454
1105	24	1.3329849291416096
1105	25	1.6282537659662992
1106	1	0.5836434402561856
1106	2	0.6838977346716426
1106	3	0.694343010838999
1106	4	1.0777173111620328
1106	5	1.0111425506069267
1106	6	0.7051730517273356
1106	7	0.6516172217252603
1106	8	0.6241675239134565
1106	9	0.7594463260808553
1106	10	0.7326642312347786
1106	11	0.7661502254737869
1106	12	0.805827967615798
1106	13	0.6634840872833863
1106	14	1.5623022739864751
1106	15	1.1123236422447178
1106	16	1.3434576857156841
1106	17	1.62192565407085
1106	18	1.741624558524265
1106	19	1.2330188513870226
1106	20	0.9366921302879874
1106	21	0.9430450082510049
1106	22	1.0889508420579475
1106	23	1.5195736876707855
1106	24	1.2203911277299753
1106	25	1.5664433584480761
1107	1	0.5795605856540185
1107	2	0.778487589080477
1107	3	0.7403553097147118
1107	4	1.0390157443690171
1107	5	0.9479591449484204
1107	6	0.7139704789672621
1107	7	0.7236380656012287
1107	8	0.7587183692456563
1107	9	0.992305010669547
1107	10	1.2578144919729795
1107	11	0.7408687092117106
1107	12	0.6885363833339753
1107	13	0.6819547376049044
1107	14	1.5504114234994688
1107	15	0.9727361846994569
1107	16	1.204948247516434
1107	17	1.572762029883009
1107	18	1.7623202095478732
1107	19	1.196465624696856
1107	20	0.9745334068485292
1107	21	0.8835560579493063
1107	22	1.0953109610239196
1107	23	1.443290102439974
1107	24	1.1675159161136506
1107	25	1.4898371914877941
1108	1	0.5204100324933449
1108	2	0.6747761612002172
1108	3	0.5294522457534505
1108	4	1.1083974249102226
1108	5	0.8739015551066274
1108	6	0.42996147058673273
1108	7	0.42053057356416446
1108	8	0.4498735296280145
1108	9	0.5459478904578532
1108	10	0.8113547696383634
1108	11	0.47401893996989486
1108	12	0.3395248517568774
1108	13	0.3784892113815843
1108	14	1.290239807496718
1108	15	0.9018860917672598
1108	16	0.8241354240541128
1108	17	1.187734684700061
1108	18	1.5871516619496049
1108	19	1.0361109562036717
1108	20	0.7370423335551948
1108	21	0.6921067213965202
1108	22	0.8968107336035231
1108	23	1.1479815523131478
1108	24	0.9045736134944846
1108	25	1.3811817579648462
1109	1	0.6745911063895669
1109	2	0.8878068569255859
1109	3	0.7787956480713518
1109	4	1.02106041728279
1109	5	1.0909006009205413
1109	6	0.7864494602126584
1109	7	0.777125617160003
1109	8	0.7652521459976285
1109	9	0.9691077949181306
1109	10	1.2998539292106344
1109	11	0.9232301576687902
1109	12	0.8645657485360942
1109	13	0.7996044931519535
1109	14	1.8148408097814013
1109	15	1.4527241837757146
1109	16	1.4444820366934934
1109	17	1.8015739298636007
1109	18	1.9954217649549904
1109	19	1.3111669919281255
1109	20	1.1518924709735643
1109	21	0.9607533602478995
1109	22	1.2940852044108837
1109	23	1.5651081545542311
1109	24	1.4057819175952346
1109	25	1.5983356535614917
1110	1	0.22310180201520424
1110	2	0.4463659399326261
1110	3	0.3861516534308933
1110	4	0.7745832657078751
1110	5	0.6881376306532516
1110	6	0.36457450728942575
1110	7	0.297107148003839
1110	8	0.3200115154035643
1110	9	0.47675263102212284
1110	10	0.8978200838686163
1110	11	0.5305198789442187
1110	12	0.3734459865389965
1110	13	0.1903749939798587
1110	14	1.0927991351699688
1110	15	0.8474733437110302
1110	16	1.0032503019400139
1110	17	1.2654669662901483
1110	18	0.8817587644294562
1110	19	0.7351533511801094
1110	20	0.5239763478866024
1110	21	0.293613737989709
1110	22	0.6048876162907106
1110	23	-0.7634716271645345
1110	24	0.21023859685731594
1110	25	0.6581359490852066
1111	1	0.22507122419482783
1111	2	0.5863309676338986
1111	3	0.41294017085947843
1111	4	0.8302263723797623
1111	5	0.7196146112591748
1111	6	0.321923979200065
1111	7	0.4485171918339148
1111	8	0.5557195833752289
1111	9	0.584455373280278
1111	10	0.9169322836865348
1111	11	0.5479678994959656
1111	12	0.48264669193263643
1111	13	0.24998976340356904
1111	14	1.1728992726885963
1111	15	0.7274672091144154
1111	16	0.7480121041349577
1111	17	1.1409462316509575
1111	18	1.3708531807145121
1111	19	0.8817900713290783
1111	20	0.44072670676474957
1111	21	0.2030760771317901
1111	22	0.694473968597535
1111	23	1.0442059012217868
1111	24	0.6515035515297001
1111	25	1.0246511201040818
1112	1	0.10989041799172838
1112	2	0.29224361977038527
1112	3	0.17078609654592825
1112	4	0.6047304975604888
1112	5	0.2418691626850631
1112	6	0.0803215539966331
1112	7	-0.044925881185186256
1112	8	0.13263742999368588
1112	9	0.4020038446349332
1112	10	0.5187974991311677
1112	11	-0.0040003332127156455
1112	12	-5.741947855710805E-4
1112	13	-0.09570435871101779
1112	14	0.6082732587126287
1112	15	0.35282818648268943
1112	16	0.29211677121472235
1112	17	0.716659109835291
1112	18	0.8479016911977695
1112	19	0.3296403075760139
1112	20	0.20451023708813665
1112	21	0.10419586068007192
1112	22	0.4910783758890531
1112	23	0.84538418523384
1112	24	0.3985624920298534
1112	25	0.7161041510310682
1113	1	-0.06379001142442675
1113	2	-0.030667646317091624
1113	3	-0.10039606682350666
1113	4	0.22222573186693495
1113	5	0.22316466007558944
1113	6	-0.09106092725782844
1113	7	-0.08550000347192821
1113	8	-0.13256914528460584
1113	9	0.06307253115804201
1113	10	0.20035380638692146
1113	11	-0.21673753159466003
1113	12	-0.1713218728043202
1113	13	-0.28625431606669594
1113	14	0.3476228084231181
1113	15	0.0668426269928446
1113	16	0.1757738618888567
1113	17	0.4809987908713822
1113	18	0.34647382337966803
1113	19	0.10636469431467077
1113	20	0.028126345709139855
1113	21	-0.055373765138689635
1113	22	0.0472478602229387
1113	23	0.18585032065654872
1113	24	0.13049336945031342
1113	25	0.48650170946663707
1114	1	-0.8097538062299421
1114	2	-0.4374003544932421
1114	3	-0.5481644345932715
1114	4	-0.18181615461943323
1114	5	-3.387562953201112
1114	6	-1.1167322738727385
1114	7	-0.7407057345885804
1114	8	-0.40194177646202434
1114	9	-0.1748764863830985
1114	10	-0.03636851142268682
1114	11	-0.43569605182099025
1114	12	-0.5153039192658042
1114	13	-0.5992553319188687
1114	14	-5.786777528758005
1114	15	-2.1568186249670376
1114	16	-1.1812788741699123
1114	17	-0.20485146777230057
1114	18	-6.209808859769268
1114	19	-2.390240911496413
1114	20	-1.3897362265195454
1114	21	-1.104204222812406
1114	22	-0.7085534824429516
1114	23	-0.17082380842436606
1114	24	-0.3696249042199753
1114	25	-0.15720856455098442
1115	1	-0.6720093736626787
1115	2	-0.2639304371802069
1115	3	-0.3122521635769182
1115	4	-2.90558361114239
1115	5	-0.9001871593359674
1115	6	-0.5698427905099024
1115	7	-0.38178632991969863
1115	8	-0.23554839400888739
1115	9	-0.053724998311139416
1115	10	-0.47058915959652126
1115	11	-0.5274186779682921
1115	12	-0.5812742028315441
1115	13	-0.503212646745105
1115	14	-6.144537350726296
1115	15	-2.353237942184272
1115	16	-1.0976386595285987
1115	17	-0.34862656574163964
1115	18	-0.059030842951194984
1115	19	-0.3440242575915917
1115	20	-0.3804992444669182
1115	21	-0.5624887442054813
1115	22	-1.7903317863112562
1115	23	-0.9931784536103336
1115	24	-0.714145330365838
1115	25	-0.16517903795205768
1116	1	0.08868611125329551
1116	2	0.36089890941973835
1116	3	0.2021825108800286
1116	4	0.49119711370069297
1116	5	-0.2641453058664993
1116	6	0.03582058842741276
1116	7	0.04466277776549532
1116	8	0.22035650287596553
1116	9	0.22474374897721106
1116	10	0.41620544891300426
1116	11	0.06680875384265761
1116	12	-0.11840650260304386
1116	13	-0.0653015600589626
1116	14	0.8338106375882859
1116	15	0.2948270218049629
1116	16	0.37327361305405304
1116	17	0.9129576184483383
1116	18	0.7928072966374984
1116	19	0.4519856345207458
1116	20	0.1404925699547377
1116	21	0.09091275407352128
1116	22	0.16146152060454
1116	23	-0.6889880449099053
1116	24	-0.21726192423967206
1116	25	0.4042711110081143
1201	1	0.647040784748814
1201	2	0.8801157254675402
1201	3	0.8130385562431179
1201	4	1.1471754077071594
1201	5	0.9758171964199462
1201	6	0.745948019681606
1201	7	0.5876098541632757
1201	8	0.7886450218687102
1201	9	0.9970137325148016
1201	10	1.237493784990079
1201	11	0.7071767192106222
1201	12	0.7518559813918344
1201	13	0.6386616129182983
1201	14	1.5068961201808122
1201	15	1.261819888093605
1201	16	1.276026493975948
1201	17	1.6192407719145905
1201	18	0.9814421359765646
1201	19	1.002400243386063
1201	20	1.0232588646928633
1201	21	0.9382177964489884
1201	22	1.2455143222629772
1201	23	1.4405665554193448
1201	24	1.1071237839488575
1201	25	1.52678193351894
1202	1	0.9146592644441895
1202	2	1.0618249323215707
1202	3	0.9846448066487774
1202	4	1.3874598563344094
1202	5	1.2628018732984216
1202	6	0.8795993902707337
1202	7	0.8737412933359465
1202	8	1.0205066215744694
1202	9	1.1504437210481484
1202	10	1.4779391453241217
1202	11	1.0670557514686863
1202	12	0.9938753016142456
1202	13	1.0458142058955175
1202	14	1.9929224934743317
1202	15	1.5700888567442206
1202	16	1.638720128903799
1202	17	1.9015197955185812
1202	18	2.05690972646574
1202	19	1.5983435105157184
1202	20	1.4724905450978767
1202	21	1.2442380578020504
1202	22	1.558951396320225
1202	23	1.8229664656333142
1202	24	1.4100596788883877
1202	25	1.772029629371474
1203	1	0.5624193593881408
1203	2	0.7318482393451333
1203	3	0.6524553763124707
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2211	11	-0.5689766271181611
2211	12	-0.7159776611391138
2211	13	-0.758992015152927
2211	14	-0.13203558571407825
2211	15	-0.4959888225196494
2211	16	-7.043993038423121
2211	17	-2.1403427199397207
2211	18	-0.8003852807656031
2211	19	-0.846668829923189
2211	20	-0.9243095869526812
2211	21	-0.8185920685891901
2211	22	-0.7793783737441231
2211	23	-0.2801462856295487
2211	24	-0.8157467727245162
2211	25	-8.06246897152144
2212	1	-1.808993828397032
2212	2	-4.3803659191457704
2212	3	-2.1224055434346774
2212	4	-4.152796023003148
2212	5	-1.5638857135226232
2212	6	-1.4316704626627228
2212	7	-1.2924189901249328
2212	8	-1.1523914780724738
2212	9	-1.3983814174281406
2212	10	-0.7612832355232868
2212	11	-1.2666816168344965
2212	12	-1.3019841436408228
2212	13	-1.5194594750369177
2212	14	-6.876101944135229
2212	15	-5.478833600033333
2212	16	-3.170889626895061
2212	17	-7.909757821009698
2212	18	-3.3803015990226477
2212	19	-2.298064656873507
2212	20	-2.2140839469224574
2212	21	-1.6801889232923912
2212	22	-1.7071255870486226
2212	23	-1.1261984996032766
2212	24	-1.5083381600767254
2212	25	-1.2968974243289821
2213	1	-0.11277946752665002
2213	2	-0.14784373816090124
2213	3	-0.1487275401746686
2213	4	0.2791714986856917
2213	5	0.18091923261655296
2213	6	-0.08804403939092253
2213	7	-0.15920274758026665
2213	8	-0.15990290342008961
2213	9	0.010332798307821633
2213	10	-3.885346438608021
2213	11	-1.387005215858487
2213	12	-0.6703118698169916
2213	13	-0.5304966992174514
2213	14	0.24848677630010485
2213	15	-0.2635037024370419
2213	16	-0.11005178864164833
2213	17	0.4130880730146629
2213	18	0.5543388541189174
2213	19	-0.13239050043397782
2213	20	-0.3737645486900334
2213	21	-0.3369028729224297
2213	22	-0.7163814496685177
2213	23	-1.0259959181038951
2213	24	-0.6109894599633492
2213	25	-0.21636450538449026
2214	1	-0.7804672217173128
2214	2	-0.5714772786847746
2214	3	-0.4577061180546842
2214	4	-0.12844328457327947
2214	5	-3.4076307681966256
2214	6	-1.3841048293710294
2214	7	-1.1020338626871897
2214	8	-0.5696874847187772
2214	9	-0.5521604618619023
2214	10	-4.265267654200443
2214	11	-1.707475767630065
2214	12	-1.2364450785936043
2214	13	-0.8509731556242102
2214	14	-0.030656405198122627
2214	15	-0.22321730898509884
2214	16	-0.28158050801018675
2214	17	0.08075396969618964
2214	18	0.4156682899786759
2214	19	-0.3034213658266367
2214	20	-0.620286692677567
2214	21	-0.6557059350040788
2214	22	-0.28990498269557463
2214	23	-0.09690675365845891
2214	24	-0.545148427037546
2214	25	-0.30578409321402233
2215	1	-2.120384196611429
2215	2	-4.6634715171080465
2215	3	-2.8353875332890013
2215	4	-1.6762545970251246
2215	5	-1.6015563085693785
2215	6	-1.9086047197669274
2215	7	-2.0648657013695875
2215	8	-2.1426906656915072
2215	9	-7.737403558030504
2215	10	-3.6817171234384993
2215	11	-2.7558097444103566
2215	12	-3.1559493531535097
2215	13	-2.4569728963368966
2215	14	-7.7025192127644075
2215	15	-4.452405821493869
2215	16	-9.263628951386579
2215	17	-4.3062353079146405
2215	18	-2.7349582119655693
2215	19	-3.2588951091011147
2215	20	-2.953543787089231
2215	21	-3.243982426887648
2215	22	-9.133720264615313
2215	23	-10.402166237275914
2215	24	-6.553585182199985
2215	25	-4.781790099355124
2216	1	-1.8252062503433102
2216	2	-1.8542584316559036
2216	3	-1.7355805809995921
2216	4	-6.876110815861825
2216	5	-3.572931279234485
2216	6	-2.5817583494175125
2216	7	-2.208188155341933
2216	8	-7.929084619649387
2216	9	-3.8644463516125107
2216	10	-2.1503840413940267
2216	11	-2.1330174965377395
2216	12	-2.34619718880095
2216	13	-2.037439112183847
2216	14	-1.4676104081171175
2216	15	-2.121855820368694
2216	16	-1.7060413405167552
2216	17	-1.3912604877013734
2216	18	-7.542840779162571
2216	19	-4.734183073275574
2216	20	-4.257550330779736
2216	21	-3.234680050841636
2216	22	-2.7988606461457444
2216	23	-2.1488304982134103
2216	24	-2.4999350775588027
2216	25	-1.9990180001340647
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96736.0
3	0.0
4	773.0
5	4789.0
6	5808.0
7	5143.0
8	4679.0
9	4534.0
10	4239.0
11	4035.0
12	3935.0
13	3823.0
14	3929.0
15	3755.0
16	3881.0
17	4188.0
18	4862.0
19	5806.0
20	7570.0
21	9441.0
22	11848.0
23	14093.0
24	16165.0
25	18505.0
26	20741.0
27	23517.0
28	25949.0
29	31269.0
30	37548.0
31	44478.0
32	56742.0
33	74152.0
34	98047.0
35	131212.0
36	186110.0
37	270109.0
38	705354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75256185876386	18.342523023942224	12.380033007798337	25.52488210949558
2	14.934408492563755	20.254408369029566	40.16801679241418	24.643166345992498
3	18.29959047362168	24.784942030089585	29.759174193867192	27.156293302421535
4	11.78205746376606	16.6944539704559	38.33430885420561	33.18917971157242
5	13.16205582423827	37.61084448726897	35.74782325824629	13.479276430246468
6	32.09760972132929	36.59928067750238	18.078215537941077	13.224894063227255
7	28.09038614999245	31.448542604466105	22.39097904977873	18.07009219576271
8	25.855196031259837	35.04325468735799	20.9051056809038	18.196443600478375
9	26.729376145441623	14.968319183647061	19.883505794670107	38.41879887624121
10	14.388095387260405	28.29035004565188	35.0274681540928	22.29408641299492
11	34.381176615580536	22.58975590092253	24.041050342385738	18.988017141111193
12	23.097769289353526	24.71340013259882	31.127547019428143	21.061283558619515
13	28.719080627882924	21.45799032683149	25.647967741040915	24.174961304244672
14	21.20494722873846	21.00646442818466	28.362036736521656	29.426551606555222
15	23.875042245303195	28.828246262638924	23.551153027126364	23.74555846493152
16	23.1120082861452	27.52031353425969	26.46780465936477	22.899873520230344
17	22.234354985007535	27.39871739690947	27.664359843819497	22.7025677742635
18	22.24668528872667	27.08292568688747	28.354731240945114	22.315657783440745
19	23.56510610850573	26.88588842122129	27.258926939091605	22.29007853118138
20	24.020823768307075	26.6972336660165	27.02390820039873	22.258034365277695
21	23.971432034496857	26.434201849337157	26.64595526831002	22.948410847855964
22	23.744550933204856	26.539362974750023	26.75868678966949	22.95739930237563
23	23.219881655832317	26.426174582811647	27.11500912591503	23.23893463544101
24	23.317449318658472	26.727675528464097	26.89743946669981	23.05743568617762
25	23.430348176939955	26.304687692598467	26.97647114412734	23.288492986334237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	272.0
1	272.0
2	400.5
3	529.0
4	529.0
5	529.0
6	1062.5
7	1596.0
8	1596.0
9	1596.0
10	2425.0
11	3254.0
12	3254.0
13	3254.0
14	5715.0
15	8176.0
16	8176.0
17	8176.0
18	14996.0
19	21816.0
20	21816.0
21	21816.0
22	33785.0
23	45754.0
24	45754.0
25	45754.0
26	63285.5
27	80817.0
28	80817.0
29	80817.0
30	103090.5
31	125364.0
32	125364.0
33	125364.0
34	146098.0
35	166832.0
36	166832.0
37	166832.0
38	185355.5
39	203879.0
40	203879.0
41	203879.0
42	219355.0
43	234831.0
44	234831.0
45	234831.0
46	246361.0
47	257891.0
48	257891.0
49	257891.0
50	253096.0
51	248301.0
52	248301.0
53	248301.0
54	226447.0
55	204593.0
56	204593.0
57	204593.0
58	177429.5
59	150266.0
60	150266.0
61	150266.0
62	123455.0
63	96644.0
64	96644.0
65	96644.0
66	75469.5
67	54295.0
68	54295.0
69	54295.0
70	40034.0
71	25773.0
72	25773.0
73	25773.0
74	18170.5
75	10568.0
76	10568.0
77	10568.0
78	7395.5
79	4223.0
80	4223.0
81	4223.0
82	2859.5
83	1496.0
84	1496.0
85	1496.0
86	977.5
87	459.0
88	459.0
89	459.0
90	280.0
91	101.0
92	101.0
93	101.0
94	63.0
95	25.0
96	25.0
97	25.0
98	17.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17332686438045658
2	0.25583168400705425
3	0.25839872879941883
4	0.44307193116212684
5	0.2542914571316355
6	0.019355517734428947
7	0.019868926692901866
8	0.017763949963162905
9	0.01909881325519249
10	0.021511835360015195
11	0.020536358338916656
12	0.027621401965842902
13	0.026851288528133527
14	0.025311061652714777
15	0.04122673936537519
16	0.021460494464167906
17	0.04040528503181852
18	0.031420628258542485
19	0.026337879569660612
20	0.029110287945414356
21	0.035373877238783935
22	0.04476926117883831
23	0.028904924362025193
24	0.04846580567984331
25	0.03640069515572977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1947765.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.67141592098805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40444394216428	61.44523479321917
2	11.144303964839697	16.420333050872433
3	2.8403390411590905	6.277553965735554
4	1.025482132130065	3.0219488270278254
5	0.523543674549416	1.9285101900266222
6	0.272047437511007	1.2025271951467444
7	0.17440940000194663	0.8994291213651371
8	0.12369572190112883	0.7290271180659949
9	0.08698211355554425	0.5767285918893403
>10	0.3812538042391725	4.715369255939438
>50	0.015311575886093961	0.7646603428092394
>100	0.006776364075301618	0.9994125375519152
>500	0.0010581209904395098	0.5233262965497774
>1k	3.527069968131671E-4	0.4959387138008435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2492	0.12794151245145077	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2229	0.11443885684361307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.134089584729164E-5
2	0.0	0.0	0.0	0.0	5.134089584729164E-5
3	0.0	0.0	0.0	0.0	5.134089584729164E-5
4	0.0	0.0	0.0	0.0	1.0268179169458328E-4
5	0.0	0.0	0.0	5.134089584729164E-5	1.0268179169458328E-4
6	0.0	0.0	0.0	5.134089584729164E-5	1.0268179169458328E-4
7	0.0	0.0	0.0	1.0268179169458328E-4	1.0268179169458328E-4
8	0.0	0.0	0.0	1.0268179169458328E-4	1.0268179169458328E-4
9	0.0	0.0	0.0	1.540226875418749E-4	1.0268179169458328E-4
10	0.0	0.0	0.0	1.540226875418749E-4	1.0268179169458328E-4
11	0.0	0.0	0.0	2.0536358338916656E-4	1.0268179169458328E-4
12	0.0	0.0	0.0	2.0536358338916656E-4	3.080453750837498E-4
13	0.0	0.0	0.0	2.0536358338916656E-4	4.107271667783331E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGT	40	0.0053278925	14.23051	6
ATACCGT	95	7.523886E-8	12.982218	6
TATACCG	60	4.0541776E-4	12.680929	5
CGTTAGG	55	0.0029453698	12.157416	1
ATTCCGT	60	0.005936734	11.068173	6
GTCTTAT	165	8.440111E-10	10.420643	1
CTAAGAC	190	9.458745E-11	10.030626	3
CCTACAC	230	0.0	9.943404	3
GGGTTAC	135	5.97749E-7	9.906043	1
GTCCTAA	350	0.0	9.825178	1
GTCCTAC	525	0.0	9.825177	1
TATACTG	185	6.5683707E-9	9.253651	5
TAGGACC	330	0.0	9.240096	4
GTATAAT	270	0.0	9.198469	1
TAGGACT	260	0.0	9.162355	4
CGCAGAT	145	1.7376169E-6	9.160103	8
CTTACAC	250	1.8189894E-12	9.147931	3
TTAGTAC	105	4.3574683E-4	9.075329	3
CTAGGAC	200	2.5120244E-9	9.052641	3
TGTACTG	390	0.0	9.022968	5
>>END_MODULE