FastQCFastQC Report
Thu 26 May 2016
SRR1513138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947765
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37230.19114215523946676No Hit
TATCAACGCAGAGTACTTTTTTTTT27220.13974991849632784No Hit
GTACATGGGAAGCAGTGGTATCAAC25740.1321514659109287No Hit
GGTATCAACGCAGAGTACTTTTTTT24180.12414228615875118No Hit
CCCATGTACTCTGCGTTGATACCAC24020.12332083182519452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG500.001475747713.3285434
TAGGACC2700.011.9886374
CTAGGAC2600.011.71830753
GTACCGT803.7020078E-410.7090586
GAATCGA803.7020078E-410.7090586
GTATTAG1803.7471182E-1010.0557751
ATTATAC1909.458745E-1110.0222373
TTTATAC2251.8189894E-129.7327053
AGGCGAG800.00443713469.5203895
AATCCCG800.0045641089.48788319
GTAGGAC5150.09.4286863
TTATACT2252.5465852E-119.3088254
TAGGGTG1856.4537744E-99.2630815
GTATTAT2401.0913936E-119.1295851
GGACTAT1151.08655484E-49.1052866
CTACACT2002.5538611E-99.0443694
GACGAAA950.00184260678.98990611
TAATAGT2651.8189894E-128.9814994
GTATCAA25700.08.97051
TTATGCT2451.6370905E-118.9375074
Produced by FastQC (version 0.11.5)