##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1947765 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.64719357828075 31.0 31.0 34.0 27.0 34.0 2 30.757862986551253 33.0 31.0 34.0 27.0 34.0 3 30.84984174168855 33.0 31.0 34.0 27.0 34.0 4 34.2347295489959 37.0 35.0 37.0 30.0 37.0 5 33.97459190405414 37.0 35.0 37.0 30.0 37.0 6 33.89174977474182 37.0 35.0 37.0 30.0 37.0 7 33.746832395078464 37.0 35.0 37.0 29.0 37.0 8 33.714889116500196 37.0 35.0 37.0 28.0 37.0 9 35.253706170918974 39.0 35.0 39.0 29.0 39.0 10 35.10022461641933 39.0 35.0 39.0 28.0 39.0 11 35.109798153267974 39.0 35.0 39.0 28.0 39.0 12 35.04763973066566 39.0 35.0 39.0 27.0 39.0 13 35.012533852903196 39.0 35.0 39.0 27.0 39.0 14 36.16679168174805 40.0 36.0 41.0 27.0 41.0 15 36.13048494043173 40.0 36.0 41.0 27.0 41.0 16 36.09218514553861 40.0 36.0 41.0 27.0 41.0 17 36.022129466337056 40.0 36.0 41.0 27.0 41.0 18 35.97941589462795 40.0 36.0 41.0 27.0 41.0 19 36.05925304130632 40.0 37.0 41.0 27.0 41.0 20 36.019836581928516 40.0 36.0 41.0 27.0 41.0 21 35.96756025495889 40.0 36.0 41.0 27.0 41.0 22 35.90654724774293 40.0 36.0 41.0 27.0 41.0 23 35.82745557087225 40.0 36.0 41.0 27.0 41.0 24 35.759853473083254 40.0 36.0 41.0 27.0 41.0 25 35.70718387485143 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.619504908216296 1101 2 0.7194711056554368 1101 3 0.6461588360215345 1101 4 0.8122774913471176 1101 5 0.7731367266892022 1101 6 0.7424787478406998 1101 7 0.7386702846289381 1101 8 0.8984827860864044 1101 9 0.8231437397270938 1101 10 1.0116346785234427 1101 11 0.982348897319639 1101 12 0.9101188821046264 1101 13 0.9167791276630979 1101 14 1.0989884148483995 1101 15 1.0312285479597492 1101 16 1.0727620082632612 1101 17 1.0966156772290176 1101 18 1.0584941997600268 1101 19 1.052614265377187 1101 20 0.975086013948264 1101 21 0.9813125172056232 1101 22 1.0127899059104166 1101 23 1.06698755669958 1101 24 1.1018265818547306 1101 25 1.066723494280339 1102 1 0.6963019357193367 1102 2 0.7517332605296012 1102 3 0.6774573025425532 1102 4 0.8079146028878768 1102 5 0.8625461592950003 1102 6 0.8775803166843943 1102 7 0.8901806136836115 1102 8 0.9074984605635521 1102 9 0.9258216858750146 1102 10 1.0278003063517431 1102 11 0.9977292889837983 1102 12 1.0455226796230477 1102 13 0.9860567336583514 1102 14 1.1956099217107834 1102 15 1.0677025932870663 1102 16 1.1363077392541499 1102 17 1.2175811338176743 1102 18 1.2603845831181388 1102 19 1.2030664450764448 1102 20 1.2032307393715342 1102 21 1.167022614993698 1102 22 1.14977743752344 1102 23 1.3368140136907414 1102 24 1.224389266966888 1102 25 1.275689611630682 1103 1 0.355901337725971 1103 2 0.3158194464047135 1103 3 0.39055835678322737 1103 4 0.4984415468247221 1103 5 0.41681491604487775 1103 6 0.23524552442116686 1103 7 0.30583869071389813 1103 8 0.3402538001796387 1103 9 0.39442007103296817 1103 10 0.4354143467086402 1103 11 0.5210096924923846 1103 12 0.4237137937907107 1103 13 0.408867218921003 1103 14 0.5288816700522219 1103 15 0.5679740391966845 1103 16 0.5436848011811861 1103 17 0.5953949625419241 1103 18 0.45464910164458416 1103 19 0.4948887859916198 1103 20 0.5153638260863787 1103 21 0.35575632156612613 1103 22 0.3156433058753123 1103 23 0.354833658529472 1103 24 0.42675062588151036 1103 25 0.4908984044820599 1104 1 0.4196613596589991 1104 2 0.46900083372355894 1104 3 0.4368584630156036 1104 4 0.563063648376648 1104 5 0.5631137407147762 1104 6 0.5360348753692747 1104 7 0.6038715380756869 1104 8 0.6189462677503883 1104 9 0.546371043944859 1104 10 0.5742999045521913 1104 11 0.6739626870690856 1104 12 0.6527493768306982 1104 13 0.7352610439868101 1104 14 0.8037319675349153 1104 15 0.7891938074810341 1104 16 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-0.8461702916359037 2216 3 -0.9266936152885066 2216 4 -0.6988933963128687 2216 5 -1.134711774580687 2216 6 -1.0694107642042 2216 7 -1.304544242813499 2216 8 -1.313124020605379 2216 9 -1.447215507175443 2216 10 -1.5578942982311688 2216 11 -1.3997718933848233 2216 12 -1.5371453614354849 2216 13 -1.5439827186487847 2216 14 -1.7024383875342437 2216 15 -1.6358176707745429 2216 16 -1.6367701024075032 2216 17 -1.8307381876077855 2216 18 -1.5855031538218824 2216 19 -1.5944288629675611 2216 20 -1.6751169288860552 2216 21 -1.9126240079342622 2216 22 -1.7259690197657136 2216 23 -1.745892403430041 2216 24 -1.8153790531006209 2216 25 -1.902971754136928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50958.0 3 1.0 4 772.0 5 6798.0 6 7792.0 7 6035.0 8 4775.0 9 4100.0 10 3734.0 11 3276.0 12 3144.0 13 2990.0 14 2987.0 15 2866.0 16 2950.0 17 3251.0 18 3541.0 19 4087.0 20 5232.0 21 7180.0 22 8812.0 23 10290.0 24 12191.0 25 13338.0 26 15024.0 27 16355.0 28 18324.0 29 22263.0 30 28196.0 31 35986.0 32 45645.0 33 59683.0 34 79210.0 35 106658.0 36 152866.0 37 251183.0 38 945272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.929581992742165 19.141558712106598 13.529361840024073 27.399497455127165 2 14.233709127448066 20.688341448411872 38.410635810370565 26.6673136137695 3 16.86241408292913 24.8377671085809 29.986501771610385 28.31331703687958 4 11.62695774279091 17.27727105865078 36.877469239596145 34.218301958962165 5 13.1729591886544 37.84885307206508 35.4171901229011 13.560997616379414 6 32.30117328533803 36.96248031603548 17.687365328715124 13.048981069911363 7 27.783098833139636 31.632849576646567 22.389020902417894 18.195030687795906 8 25.85957766700146 35.43416374492418 20.472942302517936 18.233316285556427 9 26.512967855818925 14.687815442309804 19.55914321889527 39.240073482976 10 14.727059886634356 28.720118294586378 34.304197814836115 22.248624003943153 11 34.42786989190174 22.443159210685067 23.930967031443732 19.198003865969458 12 22.76819842229427 24.9249267750473 31.148675533239377 21.15819926941905 13 28.676508716400594 21.39837198019268 25.737191088247297 24.187928215159427 14 21.68405866301114 21.02452335661679 27.347970848190332 29.94344713218174 15 23.847486734796036 28.97870122935775 23.40359334930035 23.77021868654586 16 23.229496371482185 27.701031695302053 25.98973695492013 23.079734978295637 17 22.44913364671519 27.61844509330077 27.02548786612426 22.90693339385978 18 22.41929702208605 27.299684367620824 27.811598385786375 22.469420224506752 19 23.566138625552878 26.88650838268477 27.204565232458744 22.34278775930361 20 23.870436115239773 26.657579328101694 27.09767348730468 22.37431106935385 21 23.819097747757922 26.29405857431249 26.870621866894485 23.016221811035102 22 23.661901544540303 26.598653635656067 26.67684586017608 23.062598959627554 23 23.22369174322478 26.42370365502203 26.79522850369856 23.55737609805463 24 23.13312950997682 26.653112670162983 27.024923437889072 23.188834381971134 25 23.257292098252403 26.4398726947225 26.891417264661595 23.411417942363506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 383.0 3 510.0 4 510.0 5 510.0 6 1086.0 7 1662.0 8 1662.0 9 1662.0 10 2383.5 11 3105.0 12 3105.0 13 3105.0 14 5478.5 15 7852.0 16 7852.0 17 7852.0 18 14651.5 19 21451.0 20 21451.0 21 21451.0 22 33196.5 23 44942.0 24 44942.0 25 44942.0 26 62899.0 27 80856.0 28 80856.0 29 80856.0 30 102095.5 31 123335.0 32 123335.0 33 123335.0 34 143495.0 35 163655.0 36 163655.0 37 163655.0 38 182594.5 39 201534.0 40 201534.0 41 201534.0 42 216857.5 43 232181.0 44 232181.0 45 232181.0 46 245143.5 47 258106.0 48 258106.0 49 258106.0 50 254577.5 51 251049.0 52 251049.0 53 251049.0 54 228895.5 55 206742.0 56 206742.0 57 206742.0 58 179832.0 59 152922.0 60 152922.0 61 152922.0 62 125814.0 63 98706.0 64 98706.0 65 98706.0 66 77184.5 67 55663.0 68 55663.0 69 55663.0 70 40967.5 71 26272.0 72 26272.0 73 26272.0 74 18437.5 75 10603.0 76 10603.0 77 10603.0 78 7403.5 79 4204.0 80 4204.0 81 4204.0 82 2882.0 83 1560.0 84 1560.0 85 1560.0 86 1016.0 87 472.0 88 472.0 89 472.0 90 294.0 91 116.0 92 116.0 93 116.0 94 63.5 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18790767880108739 2 0.008009179752177496 3 0.0052367713764237475 4 0.002567044792364582 5 0.08039984289685871 6 0.3318675507568931 7 0.0294696742163454 8 0.015248246066645616 9 0.005288112272271039 10 0.004363976147019789 11 0.0 12 0.0 13 0.0 14 0.0015402268754187492 15 0.0 16 0.0 17 0.020895744609847697 18 0.028904924362025193 19 0.0 20 0.0 21 8.727952294039578E-4 22 5.134089584729164E-5 23 0.00595554391828583 24 0.0 25 3.080453750837498E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1947765.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.76990396788375 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63452537325601 57.77267521129097 2 12.123703489519478 17.159866633767813 3 3.2323364849473735 6.862564278948377 4 1.183771986068476 3.3510171909574824 5 0.5816921126797046 2.0583147476609054 6 0.3238949394438481 1.3753208256074794 7 0.2051901754882409 1.0164902310119215 8 0.1339509671762504 0.7583757666774712 9 0.10158494296192881 0.6470240992198786 >10 0.45002763149296615 5.432480962753576 >50 0.018419408198429778 0.8948016292129801 >100 0.00894116594910314 1.1489690913606276 >500 0.0014528317172873351 0.6862504720548568 >1k 5.084911010505525E-4 0.8358488594755911 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3723 0.19114215523946676 No Hit TATCAACGCAGAGTACTTTTTTTTT 2722 0.13974991849632784 No Hit GTACATGGGAAGCAGTGGTATCAAC 2574 0.1321514659109287 No Hit GGTATCAACGCAGAGTACTTTTTTT 2418 0.12414228615875118 No Hit CCCATGTACTCTGCGTTGATACCAC 2402 0.12332083182519452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0268179169458328E-4 2 0.0 0.0 0.0 0.0 1.0268179169458328E-4 3 0.0 0.0 0.0 0.0 1.0268179169458328E-4 4 0.0 0.0 0.0 0.0 1.0268179169458328E-4 5 0.0 0.0 0.0 0.0 1.0268179169458328E-4 6 0.0 0.0 0.0 0.0 2.567044792364582E-4 7 0.0 0.0 0.0 5.134089584729164E-5 2.567044792364582E-4 8 0.0 0.0 0.0 5.134089584729164E-5 2.567044792364582E-4 9 0.0 0.0 0.0 5.134089584729164E-5 2.567044792364582E-4 10 0.0 0.0 0.0 5.134089584729164E-5 2.567044792364582E-4 11 0.0 0.0 0.0 1.0268179169458328E-4 2.567044792364582E-4 12 0.0 0.0 0.0 1.0268179169458328E-4 4.107271667783331E-4 13 0.0 0.0 0.0 1.540226875418749E-4 4.6206806262562473E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACACG 50 0.0014757477 13.328543 4 TAGGACC 270 0.0 11.988637 4 CTAGGAC 260 0.0 11.7183075 3 GTACCGT 80 3.7020078E-4 10.709058 6 GAATCGA 80 3.7020078E-4 10.709058 6 GTATTAG 180 3.7471182E-10 10.055775 1 ATTATAC 190 9.458745E-11 10.022237 3 TTTATAC 225 1.8189894E-12 9.732705 3 AGGCGAG 80 0.0044371346 9.520389 5 AATCCCG 80 0.004564108 9.487883 19 GTAGGAC 515 0.0 9.428686 3 TTATACT 225 2.5465852E-11 9.308825 4 TAGGGTG 185 6.4537744E-9 9.263081 5 GTATTAT 240 1.0913936E-11 9.129585 1 GGACTAT 115 1.08655484E-4 9.105286 6 CTACACT 200 2.5538611E-9 9.044369 4 GACGAAA 95 0.0018426067 8.989906 11 TAATAGT 265 1.8189894E-12 8.981499 4 GTATCAA 2570 0.0 8.9705 1 TTATGCT 245 1.6370905E-11 8.937507 4 >>END_MODULE