FastQCFastQC Report
Thu 26 May 2016
SRR1513137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472169
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58920.40022578929457153No Hit
TATCAACGCAGAGTACTTTTTTTTT34080.23149516122129998No Hit
GGTATCAACGCAGAGTACTTTTTTT31870.21648329777355724No Hit
GTACATGGGAAGCAGTGGTATCAAC24990.16974953283216804No Hit
CCCATGTACTCTGCGTTGATACCAC19610.13320481547974453No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15840.10759634253947746No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15580.10583024095739008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3050.013.7478174
CGCAGAT1405.4569682E-1112.1979958
GTCCTAC8350.011.8986051
AGGACCT6100.011.5374385
GTAAGAC1251.7447746E-811.4372453
TAGGACT2400.011.1180274
TCCTACA9100.011.110052
CGCATAG600.005934059611.0685516
AAGACCG700.001477520410.86924655
GGACCTG5700.010.652446
GTCTTAC1451.3222234E-810.541471
GGTATCA18350.010.3601531
GTAGGAC7550.010.3515913
CCTACAG9350.010.2955593
GGCGAGG3450.010.177237519
CTAGGAC1502.3221219E-810.166443
ACTGATC856.67388E-410.0454078
CTGTAGG7850.09.9791461
GTATTAG1355.9650665E-79.9070291
TTAGGAC5600.09.8714323
Produced by FastQC (version 0.11.5)