##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513137_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1472169
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.33003004410499	31.0	30.0	34.0	25.0	34.0
2	29.131496451833996	31.0	30.0	34.0	22.0	34.0
3	29.350247831600857	31.0	30.0	34.0	23.0	34.0
4	32.40032767977046	35.0	33.0	37.0	20.0	37.0
5	32.5874631241386	35.0	33.0	37.0	24.0	37.0
6	32.914783560854765	37.0	35.0	37.0	26.0	37.0
7	32.85335039659169	37.0	35.0	37.0	26.0	37.0
8	32.7099049090152	37.0	35.0	37.0	25.0	37.0
9	33.872013335425486	39.0	34.0	39.0	22.0	39.0
10	33.46046819352941	38.0	33.0	39.0	17.0	39.0
11	33.89889679785405	39.0	34.0	39.0	23.0	39.0
12	33.884033694501106	39.0	34.0	39.0	23.0	39.0
13	33.90581651970664	39.0	35.0	39.0	23.0	39.0
14	34.10556260864072	39.0	33.0	41.0	16.0	41.0
15	34.52074388198638	39.0	34.0	41.0	17.0	41.0
16	34.38950419415162	39.0	34.0	41.0	17.0	41.0
17	34.02099351365231	39.0	33.0	41.0	16.0	41.0
18	33.76967318290223	39.0	33.0	41.0	11.0	41.0
19	34.40796063495427	39.0	34.0	41.0	18.0	41.0
20	34.635319722124294	39.0	34.0	41.0	18.0	41.0
21	34.68876127672842	40.0	34.0	41.0	18.0	41.0
22	34.369415467925215	40.0	34.0	41.0	16.0	41.0
23	33.91534667555152	39.0	34.0	41.0	10.0	41.0
24	34.16963541549917	39.0	34.0	41.0	8.0	41.0
25	33.81984269469062	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.156259140318248
1101	2	1.2779978427299845
1101	3	1.258909613374783
1101	4	1.7236259056794694
1101	5	1.518009535604449
1101	6	1.2113100856832943
1101	7	1.239947395890546
1101	8	1.385686747143069
1101	9	1.7373291681902359
1101	10	2.0801207577000227
1101	11	1.6166274474725384
1101	12	1.6127440670949085
1101	13	1.3400196605760684
1101	14	2.5470249990664584
1101	15	2.082867357001028
1101	16	2.107496974337259
1101	17	2.4424575277333744
1101	18	2.4178693197916132
1101	19	2.0675550869089534
1101	20	1.720427720018371
1101	21	1.7751129704561635
1101	22	1.9777440777352098
1101	23	2.15537655274111
1101	24	1.848697381544305
1101	25	2.2327683328110126
1102	1	0.6608853944477708
1102	2	0.8554465623521317
1102	3	0.7610026675990618
1102	4	0.9679056599010707
1102	5	1.0340813353849754
1102	6	0.7722648787884552
1102	7	0.7464671773954592
1102	8	0.6305428878455359
1102	9	1.077852939353157
1102	10	1.3182712141286785
1102	11	0.9834300912472358
1102	12	0.7666502446483108
1102	13	0.6583245349642439
1102	14	1.7265457654605143
1102	15	1.3437871669846615
1102	16	1.4139908700901884
1102	17	1.657919715857311
1102	18	1.9205040410671046
1102	19	1.4252909054502751
1102	20	1.0075563045247975
1102	21	1.0660576158191617
1102	22	1.1130986532066771
1102	23	1.5390893167021673
1102	24	1.2887977954086978
1102	25	1.6601080378189224
1103	1	0.9225226329360225
1103	2	1.2998156418636846
1103	3	1.0072945652641678
1103	4	1.5373566249588748
1103	5	1.413651476867166
1103	6	1.154596245181402
1103	7	1.0805329988219086
1103	8	1.1744736134115854
1103	9	1.5513612515006443
1103	10	1.7178716253174997
1103	11	1.2733833832676495
1103	12	1.2979023669234167
1103	13	1.4005914390304497
1103	14	2.2672491565028565
1103	15	1.866810872771211
1103	16	1.9924404924992203
1103	17	2.258178206328637
1103	18	2.4889744886630965
1103	19	1.979607017193409
1103	20	1.5905756993247024
1103	21	1.6984947532396646
1103	22	1.9493058370314529
1103	23	2.3230521511041786
1103	24	1.9195035781628107
1103	25	2.0645521926145562
1104	1	0.876636426683671
1104	2	1.0698144687235782
1104	3	0.9023701359167937
1104	4	1.4551184505586434
1104	5	1.257699454716935
1104	6	1.1027872610343792
1104	7	1.049468164612719
1104	8	0.9956478866386504
1104	9	1.3406775045880366
1104	10	1.4545627510075647
1104	11	1.1269769725729901
1104	12	1.024387384323589
1104	13	1.0618658735695448
1104	14	2.1343233261271237
1104	15	1.609521660457922
1104	16	1.628629526861701
1104	17	2.1885368607432554
1104	18	2.163395885222151
1104	19	1.5070996713776097
1104	20	1.2980465798600775
1104	21	1.1857925103095184
1104	22	1.542095637915665
1104	23	1.756045315930784
1104	24	1.5371359371158775
1104	25	1.7544409254357944
1105	1	0.46739682474332156
1105	2	0.6779983226201871
1105	3	0.6079346291784411
1105	4	1.0492122115326907
1105	5	0.9398930086658126
1105	6	0.7190184311047716
1105	7	0.5670211432353369
1105	8	0.733751546280665
1105	9	0.9318192870328588
1105	10	1.3179445254401472
1105	11	0.6451549567420543
1105	12	0.6930953554474044
1105	13	0.5079271342551763
1105	14	1.2203606853159883
1105	15	0.93957846877138
1105	16	1.1220293584186862
1105	17	1.5336510626525879
1105	18	1.1645184864622777
1105	19	0.9433311457103386
1105	20	0.8186126007721981
1105	21	0.7382665645501945
1105	22	1.0553399238056258
1105	23	1.3137976878035929
1105	24	1.0966693001147902
1105	25	1.2511145351999104
1106	1	0.5932759143851918
1106	2	0.7914537188175785
1106	3	0.7525333764498008
1106	4	1.133844324016387
1106	5	1.00006600057894
1106	6	0.6989586952750386
1106	7	0.8023171621174257
1106	8	0.8002213311222803
1106	9	0.9187296982056523
1106	10	0.8180228416985997
1106	11	0.7765654947125924
1106	12	0.7154846891873774
1106	13	0.5808582176052255
1106	14	1.5896558224150255
1106	15	1.2112364363940316
1106	16	1.2808319401203647
1106	17	1.6312161314465854
1106	18	1.6848637244193938
1106	19	1.3153433182945093
1106	20	1.0348885425148424
1106	21	0.8218486590501541
1106	22	1.0181235405416018
1106	23	1.4288674762916784
1106	24	1.0235310308958177
1106	25	1.224148051243553
1107	1	0.7437398570320042
1107	2	0.9139215923122528
1107	3	0.8349304882705617
1107	4	1.189508624479366
1107	5	1.1129071443081742
1107	6	0.9194685191884489
1107	7	0.8485390915032411
1107	8	0.9733541783899256
1107	9	1.0924781431801946
1107	10	1.4488076967125139
1107	11	0.8937118145925496
1107	12	0.9603362724277318
1107	13	0.8260705026269619
1107	14	1.5949348933754877
1107	15	0.9751450970842725
1107	16	1.2103283683711652
1107	17	1.5995232698149948
1107	18	1.8358286417625749
1107	19	1.1661137772995502
1107	20	0.9398267903698709
1107	21	0.9415671834111521
1107	22	1.0474221439043063
1107	23	1.3539505178573492
1107	24	1.1843487177874295
1107	25	1.5768815294988485
1108	1	0.5859914244648294
1108	2	0.7595891668562551
1108	3	0.5395603463633236
1108	4	0.9013141141320986
1108	5	0.8556848535470181
1108	6	0.5808630915370969
1108	7	0.4858211376752877
1108	8	0.5045197568691222
1108	9	0.68342127589478
1108	10	1.0732284108671806
1108	11	0.627028382246813
1108	12	0.45404558402261586
1108	13	0.5385458286087044
1108	14	1.3139207595916957
1108	15	0.9658116751775339
1108	16	0.9996130432751613
1108	17	1.3300952483549224
1108	18	1.680578848961062
1108	19	0.8312802320227277
1108	20	0.7797208818914996
1108	21	0.7506515717169364
1108	22	1.0349807446976698
1108	23	1.2143635862296875
1108	24	0.9184517697736894
1108	25	1.3125330342976298
1109	1	0.43847050240346164
1109	2	0.7170211716201571
1109	3	0.7129018588705129
1109	4	0.8605485809209839
1109	5	0.9186430297161152
1109	6	0.7014520544912131
1109	7	0.5423401655711118
1109	8	0.7724981351293749
1109	9	0.8567940066227919
1109	10	1.1138327800132757
1109	11	0.8583764708722157
1109	12	0.8301997801807488
1109	13	0.836035181244263
1109	14	1.6883922936472615
1109	15	1.2725381091279786
1109	16	1.48094181562643
1109	17	1.7171250921935766
1109	18	1.8130221508186253
1109	19	1.2016375948010847
1109	20	1.163258967730684
1109	21	1.0875796831784683
1109	22	1.257358667796268
1109	23	1.5933773714155492
1109	24	1.261596209457359
1109	25	1.3374139562965652
1110	1	0.24471864809817134
1110	2	0.5407542305732171
1110	3	0.498447800493377
1110	4	0.7338836898346202
1110	5	0.6741331012628962
1110	6	0.33433868520616983
1110	7	0.2796096721039518
1110	8	0.32666661341911407
1110	9	0.5419043240343342
1110	10	0.9530338751062217
1110	11	0.5053216760086556
1110	12	0.4943836695969992
1110	13	0.44270772098425226
1110	14	1.1657961372474688
1110	15	0.8128441615045574
1110	16	0.9858576690545533
1110	17	1.1724039910909738
1110	18	0.7582515686938578
1110	19	0.5370356093380835
1110	20	0.3783682455930659
1110	21	0.285875201962817
1110	22	0.6632900924566485
1110	23	-0.6206593497098325
1110	24	0.2587833563805546
1110	25	0.6302041138148198
1111	1	0.03366244047053257
1111	2	0.4332966952192585
1111	3	0.3930127285027787
1111	4	0.6192852629113972
1111	5	0.5486173246317492
1111	6	0.38282485795527066
1111	7	0.40144892057088555
1111	8	0.5157591075594112
1111	9	0.7006154958326007
1111	10	0.8801248911778004
1111	11	0.6080373849260639
1111	12	0.4016598395048021
1111	13	0.18899036322226692
1111	14	1.144878332634434
1111	15	0.7269196712779333
1111	16	0.7735780649860615
1111	17	1.2591484291302848
1111	18	1.3888596724181923
1111	19	0.8787036494204372
1111	20	0.5218505367338224
1111	21	0.2357047616967094
1111	22	0.670581251860618
1111	23	1.1139546036467323
1111	24	0.870378290974898
1111	25	1.0151216976870145
1112	1	0.11629164954565852
1112	2	0.3245749443796946
1112	3	0.17236951117726917
1112	4	0.6508550777718227
1112	5	0.3620685191971731
1112	6	0.013675087075263548
1112	7	-0.1030586299911107
1112	8	-0.017896901254367492
1112	9	0.21836694894153652
1112	10	0.4962747903708973
1112	11	0.04369721658517989
1112	12	-0.01932915949081604
1112	13	-0.10501549788396858
1112	14	0.46710348819562597
1112	15	0.356189584511732
1112	16	0.2521136438477356
1112	17	0.6250188417527553
1112	18	0.7485743748866724
1112	19	0.31959813918415136
1112	20	0.2508647086940172
1112	21	0.286044422684661
1112	22	0.4796052759067848
1112	23	0.8642573193122871
1112	24	0.574838572172311
1112	25	0.8106131147208018
1113	1	-0.09141680645905126
1113	2	0.00847485185042629
1113	3	0.012041891739539068
1113	4	0.29553226766163476
1113	5	0.2674798355631509
1113	6	0.1449223669708104
1113	7	-0.060720272769621886
1113	8	-0.015458407616215197
1113	9	0.12261488349079741
1113	10	0.5275569973219305
1113	11	-0.010568455788067865
1113	12	0.043224831153857224
1113	13	-0.21355488865371797
1113	14	0.5397718786618171
1113	15	0.17302424239117187
1113	16	0.31884845789316074
1113	17	0.6025731798393394
1113	18	0.5067160360978917
1113	19	0.271536133603135
1113	20	0.08007085878161035
1113	21	-0.056303077745070595
1113	22	0.17926898870764774
1113	23	0.22315238088265232
1113	24	0.16691177635478027
1113	25	0.5466521671918443
1114	1	-1.10704345492152
1114	2	-0.7964739710088438
1114	3	-0.6713433797054336
1114	4	-0.5491108665337414
1114	5	-3.6986115961999246
1114	6	-1.6435593409138818
1114	7	-1.2725231532292192
1114	8	-0.8487162349038933
1114	9	-0.5744146702551731
1114	10	-0.2737861532749477
1114	11	-0.7441636222190553
1114	12	-0.9093561900838836
1114	13	-0.799028471142222
1114	14	-6.151455973760061
1114	15	-2.5684276901055405
1114	16	-1.373985855836672
1114	17	-0.5539787796976938
1114	18	-6.439448005588542
1114	19	-2.630297732409833
1114	20	-1.5581340182292323
1114	21	-1.2631881652271773
1114	22	-1.0640079441215278
1114	23	-0.5267405660252251
1114	24	-0.5539939415658637
1114	25	-0.37643805440584543
1115	1	-1.0111345856413152
1115	2	-0.6903519193656216
1115	3	-0.6323807137069366
1115	4	-3.077871016059362
1115	5	-1.2845895059563368
1115	6	-0.9295695869404135
1115	7	-0.9302902914523834
1115	8	-0.8967785981942669
1115	9	-0.7578854186282058
1115	10	-0.973023626779522
1115	11	-1.0165123866688006
1115	12	-1.0693651968355624
1115	13	-1.1100607261484186
1115	14	-6.536732812864262
1115	15	-2.792796201689036
1115	16	-1.6343449746648488
1115	17	-0.7664165620051833
1115	18	-0.6012930691437006
1115	19	-0.9473296352723537
1115	20	-1.045879079752801
1115	21	-1.1419437436570163
1115	22	-2.4530803164186565
1115	23	-1.73717383818488
1115	24	-1.2917974115354625
1115	25	-0.838490002369845
1116	1	-0.13870603449691998
1116	2	-0.03834508824013838
1116	3	-0.06073271220522258
1116	4	0.13015387176313453
1116	5	-0.49775142540994466
1116	6	-0.172748238259409
1116	7	-0.13086992033106526
1116	8	-0.15928673550025252
1116	9	-0.159683477467091
1116	10	0.058628538134939845
1116	11	-0.15336668166223433
1116	12	-0.47971915911696783
1116	13	-0.3896856339456747
1116	14	0.4209202557273599
1116	15	0.02726961272142603
1116	16	0.01702305537911286
1116	17	0.48534807521837564
1116	18	0.46395112825019424
1116	19	0.004872039959792573
1116	20	-0.4480879146109089
1116	21	-0.4931841638969843
1116	22	-0.41903990484244247
1116	23	-1.1190240660294464
1116	24	-0.6688210587572243
1116	25	-0.20173828857198117
1201	1	1.055044165989628
1201	2	1.3028266224093947
1201	3	1.2035758187431753
1201	4	1.5831641363295006
1201	5	1.4592813322812361
1201	6	1.236679268736495
1201	7	1.2405506833740318
1201	8	1.4058454373048406
1201	9	1.590209682604474
1201	10	2.0563987827923427
1201	11	1.6501782309463238
1201	12	1.4672782336093633
1201	13	1.4611960067293097
1201	14	2.2742786166804123
1201	15	1.8797232403983344
1201	16	2.1397565226115915
1201	17	2.4248344226449206
1201	18	1.7185155817868107
1201	19	1.6748027138670878
1201	20	1.6529404046098932
1201	21	1.6637980970532027
1201	22	1.839543247664956
1201	23	2.178855657218058
1201	24	1.7901220554913095
1201	25	2.255409500971126
1202	1	0.9598292631011525
1202	2	1.0136360611803035
1202	3	1.090810749514013
1202	4	1.395553060006911
1202	5	1.1434376074868098
1202	6	0.8800883036169296
1202	7	0.7700294482010293
1202	8	0.9162377573730112
1202	9	1.0949538065650017
1202	10	1.5020999089956604
1202	11	1.2037345200673073
1202	12	0.8663519998837401
1202	13	0.9657837970739109
1202	14	1.7915191222049103
1202	15	1.3782896096016373
1202	16	1.4933238925218362
1202	17	1.8292273972527298
1202	18	1.899905954556587
1202	19	1.409275221054564
1202	20	1.1395077393021253
1202	21	1.095441223485011
1202	22	1.2851249762165438
1202	23	1.6615395618133206
1202	24	1.173060818239584
1202	25	1.5447851715091971
1203	1	0.7865298322707268
1203	2	0.9865604623446416
1203	3	0.7967864849604389
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2211	10	-0.452954821860466
2211	11	-0.6824266900717575
2211	12	-0.6500889148338871
2211	13	-0.6692646152444581
2211	14	-0.09577823255316531
2211	15	-0.38460169764023533
2211	16	-6.9210507808713615
2211	17	-2.2042286640728506
2211	18	-0.8711404390088333
2211	19	-0.7556970310272746
2211	20	-0.777189890848824
2211	21	-0.7481910614611706
2211	22	-0.603866859358412
2211	23	-0.2763092741252464
2211	24	-0.761429853969382
2211	25	-7.8310947047428385
2212	1	-1.5305092042946562
2212	2	-3.9442083617970525
2212	3	-1.8167174049292711
2212	4	-3.5052400196591407
2212	5	-1.321307498995953
2212	6	-1.0702321483125985
2212	7	-0.9698015426227506
2212	8	-0.8707999726321844
2212	9	-0.9291895664568557
2212	10	-0.4374824576470999
2212	11	-0.8262506384737804
2212	12	-1.0312417417566664
2212	13	-1.2174995849825478
2212	14	-6.581274952264899
2212	15	-4.985743513679186
2212	16	-2.7781468089337977
2212	17	-7.3863184072813795
2212	18	-2.8258056928223247
2212	19	-1.8212141325878122
2212	20	-1.9066479697469418
2212	21	-1.5495936426213888
2212	22	-1.4329700129584424
2212	23	-0.8028079302027251
2212	24	-1.1694007607273775
2212	25	-1.1476439189784173
2213	1	-0.15420549132010208
2213	2	-0.22622755245203763
2213	3	-0.23223680417833492
2213	4	0.15725476548896467
2213	5	0.020369597374511272
2213	6	-0.35183415081572633
2213	7	-0.31481614420806636
2213	8	-0.3730945074673926
2213	9	-0.04266474376181861
2213	10	-4.316915081760573
2213	11	-1.7369723739764922
2213	12	-0.9916088673302994
2213	13	-0.7160394264510543
2213	14	0.27145763012975976
2213	15	-0.3612128503500216
2213	16	-0.2908710475654175
2213	17	0.20066532238070067
2213	18	0.3548367769315348
2213	19	-0.3289321133971512
2213	20	-0.6788632388333014
2213	21	-0.5883921407440411
2213	22	-0.7984268339847205
2213	23	-1.2191617057554964
2213	24	-0.5006481533014266
2213	25	-0.1818533474306463
2214	1	-0.8728574294311464
2214	2	-0.6196416265801084
2214	3	-0.6868668226283852
2214	4	-0.2909191037480454
2214	5	-3.4100621393157695
2214	6	-1.3594511714078443
2214	7	-1.0764906532590643
2214	8	-0.8299074911566748
2214	9	-0.7154088732674282
2214	10	-4.258069306877218
2214	11	-1.8110645106129724
2214	12	-1.2290204116348988
2214	13	-0.9084684026494259
2214	14	-0.2523013891255772
2214	15	-0.6179509181357403
2214	16	-0.5892988403046999
2214	17	-0.2996772578818181
2214	18	0.10568527055375654
2214	19	-0.6017047171064007
2214	20	-0.856134321807879
2214	21	-0.8142054095175908
2214	22	-0.5991664445794527
2214	23	-0.32595074989496453
2214	24	-1.1053016321680573
2214	25	-0.6939547128576962
2215	1	-2.1167683792531875
2215	2	-4.516107692889758
2215	3	-2.821114956443303
2215	4	-1.8163050146267672
2215	5	-1.8332233100251223
2215	6	-1.9981479955025385
2215	7	-2.128976902941396
2215	8	-2.1343642795407014
2215	9	-7.974625240128667
2215	10	-3.6997962045658994
2215	11	-2.942180060684933
2215	12	-3.2641971280708866
2215	13	-2.557076845482733
2215	14	-7.444793656423588
2215	15	-4.429853024564583
2215	16	-8.737733799359898
2215	17	-4.185285280667117
2215	18	-2.747852613431352
2215	19	-3.273680799388405
2215	20	-2.9392159335808117
2215	21	-3.274388413506074
2215	22	-9.030802643500497
2215	23	-10.315072625705596
2215	24	-6.510237788820948
2215	25	-4.825550223742731
2216	1	-2.1922006160695346
2216	2	-2.234682131092004
2216	3	-2.0099925354277595
2216	4	-6.9016115320679425
2216	5	-3.6549828185010256
2216	6	-2.9831218630983543
2216	7	-2.491491828752771
2216	8	-8.124406805868851
2216	9	-4.30356871290007
2216	10	-2.5669821752819466
2216	11	-2.563094425677299
2216	12	-2.6946569164669256
2216	13	-2.507989889173764
2216	14	-1.9324933197364302
2216	15	-2.4794880762421485
2216	16	-2.0764218694769525
2216	17	-1.8764892064001941
2216	18	-7.503626577922603
2216	19	-5.088937792498061
2216	20	-4.639595563029193
2216	21	-3.738962244888448
2216	22	-3.333488922520189
2216	23	-2.436962062146108
2216	24	-2.746948311273286
2216	25	-2.232615637946232
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69762.0
3	1.0
4	550.0
5	3477.0
6	4879.0
7	4301.0
8	4053.0
9	3542.0
10	3318.0
11	3246.0
12	3189.0
13	3187.0
14	3014.0
15	2976.0
16	3046.0
17	3195.0
18	3665.0
19	4323.0
20	5757.0
21	7575.0
22	9480.0
23	11198.0
24	13224.0
25	15047.0
26	16661.0
27	18410.0
28	20702.0
29	23971.0
30	28813.0
31	34252.0
32	43051.0
33	55724.0
34	72717.0
35	97546.0
36	138223.0
37	201408.0
38	534686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7944432686831	18.571456763776993	12.151232700713237	24.482867266826677
2	14.584251343217305	20.655954527096927	41.51588602624935	23.243908103436418
3	18.34260043751992	26.285657464945285	29.704156439252564	25.66758565828223
4	11.059485452643203	16.707603963558412	39.521678567943056	32.71123201585532
5	12.140192778009713	38.740779434469715	35.75655459535074	13.362473192169833
6	30.820159740849146	37.650861073291104	18.47217579646222	13.056803389397533
7	27.187438217316068	32.29494263509643	22.661701631844164	17.855917515743332
8	25.440274688468545	35.6641860098565	21.113618792300134	17.781920509374817
9	27.034706166505078	14.414102866351739	20.37943542606655	38.17175554107663
10	13.975030369571185	28.86107993379841	35.47763794035878	21.68625175627162
11	33.68553715498939	23.05576220806794	24.194377919320594	19.06432271762208
12	23.031289684548394	25.697615716660906	31.79390975313281	19.477184845657895
13	28.7629247027777	22.331114272785456	25.889696520029297	23.016264504407545
14	20.739373317552353	21.904737962813236	29.257229514055442	28.09865920557897
15	23.44659296930424	29.917967061791078	24.12789038355427	22.507549585350407
16	21.75008067940552	28.553171974522296	27.624220806794053	22.07252653927813
17	20.826894771820097	28.328311595118787	28.511921022124188	22.332872610936924
18	20.837472430554897	27.585344674119987	30.21855792267374	21.358624972651384
19	22.617249525579954	26.917706721782963	28.935308333961817	21.529735418675266
20	23.82508017611567	26.822919497744195	28.558052943414687	20.793947382725445
21	23.574734332966628	26.698865848595442	27.540832780108083	22.185567038329847
22	22.90727314365864	27.46679152477987	28.126645813187164	21.499289518374326
23	21.99034627834354	27.42790284311887	28.582775499332776	21.99897537920482
24	22.629268017535882	26.933184369387092	28.67562760916264	21.76192000391438
25	22.423636630498596	27.02935756376525	28.3730212659549	22.17398453978125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	192.0
2	411.0
3	630.0
4	630.0
5	630.0
6	1547.5
7	2465.0
8	2465.0
9	2465.0
10	3103.0
11	3741.0
12	3741.0
13	3741.0
14	5702.5
15	7664.0
16	7664.0
17	7664.0
18	13585.5
19	19507.0
20	19507.0
21	19507.0
22	30384.0
23	41261.0
24	41261.0
25	41261.0
26	57845.0
27	74429.0
28	74429.0
29	74429.0
30	93849.0
31	113269.0
32	113269.0
33	113269.0
34	127280.0
35	141291.0
36	141291.0
37	141291.0
38	152429.5
39	163568.0
40	163568.0
41	163568.0
42	170813.0
43	178058.0
44	178058.0
45	178058.0
46	184912.0
47	191766.0
48	191766.0
49	191766.0
50	185123.0
51	178480.0
52	178480.0
53	178480.0
54	159277.5
55	140075.0
56	140075.0
57	140075.0
58	119448.0
59	98821.0
60	98821.0
61	98821.0
62	79947.5
63	61074.0
64	61074.0
65	61074.0
66	46526.5
67	31979.0
68	31979.0
69	31979.0
70	23293.0
71	14607.0
72	14607.0
73	14607.0
74	10258.0
75	5909.0
76	5909.0
77	5909.0
78	4070.0
79	2231.0
80	2231.0
81	2231.0
82	1507.5
83	784.0
84	784.0
85	784.0
86	527.0
87	270.0
88	270.0
89	270.0
90	172.5
91	75.0
92	75.0
93	75.0
94	46.0
95	17.0
96	17.0
97	17.0
98	11.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1815009010514418
2	0.2626057198596085
3	0.26443974842562235
4	0.47005472877094956
5	0.2613830341489326
6	0.01935919041903477
7	0.01874784756369683
8	0.016777965029830134
9	0.020242241210078463
10	0.02044602216185778
11	0.01997053327437271
12	0.027578355474133745
13	0.025472618972414172
14	0.024114079293885416
15	0.03837874591843735
16	0.01997053327437271
17	0.03851459988629023
18	0.029276530072294686
19	0.02526883802063486
20	0.02778213642591306
21	0.03369178402751314
22	0.042929853841508686
23	0.027510428490207305
24	0.0456469331985662
25	0.035389958625674095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1472169.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.55520111843168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55816692517088	68.7814941236801
2	9.207495888763583	14.465933826779127
3	1.8382128037610468	4.332035294937757
4	0.5811161274913449	1.8259877707298713
5	0.249683327816752	0.980696201628213
6	0.14654588125816967	0.6907164705187996
7	0.08320149385614634	0.45751370582564743
8	0.057921553648619784	0.3640031436767486
9	0.04141094400882299	0.2927740531605483
>10	0.20959039184075567	2.8378694982806394
>50	0.012977125336636173	0.7252555868947836
>100	0.0114857071531601	1.8219131219127394
>500	0.0011397515448530687	0.6220505966595196
>1k	9.64405153337212E-4	1.3959636341554256
>5k	8.767319575792837E-5	0.4057929711600852
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5892	0.40022578929457153	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3408	0.23149516122129998	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3187	0.21648329777355724	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2499	0.16974953283216804	No Hit
CCCATGTACTCTGCGTTGATACCAC	1961	0.13320481547974453	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1584	0.10759634253947746	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1558	0.10583024095739008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.792698392643779E-5	0.0
8	0.0	0.0	0.0	6.792698392643779E-5	0.0
9	0.0	0.0	0.0	1.3585396785287558E-4	0.0
10	0.0	0.0	0.0	2.0378095177931338E-4	0.0
11	0.0	0.0	0.0	2.0378095177931338E-4	0.0
12	0.0	0.0	0.0	2.0378095177931338E-4	1.3585396785287558E-4
13	0.0	0.0	0.0	2.7170793570575116E-4	1.3585396785287558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	305	0.0	13.747817	4
CGCAGAT	140	5.4569682E-11	12.197995	8
GTCCTAC	835	0.0	11.898605	1
AGGACCT	610	0.0	11.537438	5
GTAAGAC	125	1.7447746E-8	11.437245	3
TAGGACT	240	0.0	11.118027	4
TCCTACA	910	0.0	11.11005	2
CGCATAG	60	0.0059340596	11.068551	6
AAGACCG	70	0.0014775204	10.8692465	5
GGACCTG	570	0.0	10.65244	6
GTCTTAC	145	1.3222234E-8	10.54147	1
GGTATCA	1835	0.0	10.360153	1
GTAGGAC	755	0.0	10.351591	3
CCTACAG	935	0.0	10.295559	3
GGCGAGG	345	0.0	10.1772375	19
CTAGGAC	150	2.3221219E-8	10.16644	3
ACTGATC	85	6.67388E-4	10.045407	8
CTGTAGG	785	0.0	9.979146	1
GTATTAG	135	5.9650665E-7	9.907029	1
TTAGGAC	560	0.0	9.871432	3
>>END_MODULE