FastQCFastQC Report
Thu 26 May 2016
SRR1513137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472169
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89190.6058407696398987No Hit
TATCAACGCAGAGTACTTTTTTTTT61900.4204680305046499No Hit
GGTATCAACGCAGAGTACTTTTTTT53860.36585473542779395No Hit
GTACATGGGAAGCAGTGGTATCAAC28890.19624105656347876No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28550.1939315391099799No Hit
CCCATGTACTCTGCGTTGATACCAC25700.17457234869094512No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24740.1680513582340071No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18240.12389881868182252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCTG1001.4036414E-712.3757195
CGTGCGC550.003099627812.07452610
GGCGAGG3900.011.67646519
TCGAACT752.0999499E-411.38455319
GTCCTAC7200.011.3778331
TGGCGAG8900.011.09097918
CCATCGA600.005933501311.0686919
TCCTACA8200.011.0324032
TAAGGCC951.32894E-511.0229094
CTAGGAC2350.010.9394893
TTATACT1855.456968E-1210.8062424
TATGGCG9950.010.58866516
TAGGACC4050.010.5775384
GCGCCAC1002.4215442E-510.440804513
GACGTGA5050.010.3367287
CATTATA1951.4551915E-1110.2552212
ATGGCGA10100.010.24380117
GTCTTAC1409.307951E-810.206031
GATCTAA750.002588904210.160671
AGGACGT8450.010.1394165
Produced by FastQC (version 0.11.5)