##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513137_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1472169 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.69485364791678 31.0 31.0 34.0 27.0 34.0 2 30.74660382062114 31.0 31.0 34.0 27.0 34.0 3 30.852804942910765 33.0 31.0 34.0 27.0 34.0 4 34.23274909334458 37.0 35.0 37.0 30.0 37.0 5 34.00287331142009 37.0 35.0 37.0 30.0 37.0 6 33.904348617583985 37.0 35.0 37.0 30.0 37.0 7 33.7356730103677 37.0 35.0 37.0 28.0 37.0 8 33.6986806541912 37.0 35.0 37.0 28.0 37.0 9 35.203610455049656 39.0 35.0 39.0 28.0 39.0 10 35.06117368318447 39.0 35.0 39.0 28.0 39.0 11 35.07234087934198 39.0 35.0 39.0 28.0 39.0 12 35.01265411783566 39.0 35.0 39.0 27.0 39.0 13 34.97404577871155 39.0 35.0 39.0 27.0 39.0 14 36.10943376745469 40.0 36.0 41.0 27.0 41.0 15 36.08601458120637 40.0 36.0 41.0 27.0 41.0 16 36.063841855113104 40.0 36.0 41.0 27.0 41.0 17 36.01553286341446 40.0 36.0 41.0 27.0 41.0 18 35.98207814456085 40.0 36.0 41.0 27.0 41.0 19 36.08189888525027 40.0 37.0 41.0 27.0 41.0 20 36.059440865824506 40.0 37.0 41.0 27.0 41.0 21 36.00815055880133 40.0 36.0 41.0 27.0 41.0 22 35.95025639040083 40.0 36.0 41.0 27.0 41.0 23 35.87657802874534 40.0 36.0 41.0 27.0 41.0 24 35.81841215240913 40.0 36.0 41.0 27.0 41.0 25 35.76680055075199 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6163277819524744 1101 2 0.631835450857551 1101 3 0.5219381689787603 1101 4 0.7144775801446812 1101 5 0.5517337682077681 1101 6 0.642483685555014 1101 7 0.6508691020626074 1101 8 0.6375055473992148 1101 9 0.7691860086036186 1101 10 0.7275777344474861 1101 11 0.7228563508228092 1101 12 0.6751210296363439 1101 13 0.8196818222112014 1101 14 0.8145860747349616 1101 15 0.9812784765352447 1101 16 0.8464068842334953 1101 17 0.8982839505039095 1101 18 0.824872409634331 1101 19 0.9390083249767045 1101 20 0.9966932060685991 1101 21 0.964633654307562 1101 22 0.9765284819178532 1101 23 1.0155787395688591 1101 24 1.046433605009888 1101 25 0.9827796224787022 1102 1 0.15079868201633317 1102 2 0.0971487629461123 1102 3 0.03403832969895504 1102 4 0.2738730753206582 1102 5 0.10712554181247924 1102 6 0.07556835745420898 1102 7 0.10703911827526014 1102 8 0.18253291202310606 1102 9 0.08650841102993212 1102 10 0.23233451605304367 1102 11 0.2088699142536683 1102 12 0.20761314195326008 1102 13 0.23522966624449282 1102 14 0.32957959450723706 1102 15 0.13027229427933662 1102 16 -0.031722863593572015 1102 17 0.269809095940964 1102 18 0.1851089060834994 1102 19 0.12306634949389661 1102 20 0.1377670384739602 1102 21 -0.003029372092832716 1102 22 0.040878967088922025 1102 23 0.04474657636820467 1102 24 0.13196008560678507 1102 25 0.12785453953662085 1103 1 0.5458947027056951 1103 2 0.6009217198990946 1103 3 0.6017876783054987 1103 4 0.7286129882657235 1103 5 0.7004712681778713 1103 6 0.6575829802196722 1103 7 0.6697091375565876 1103 8 0.7726960166421435 1103 9 0.7724483938281708 1103 10 0.8057003800409888 1103 11 0.7966594453052167 1103 12 0.797525053563696 1103 13 0.8704162176534282 1103 14 0.9175616857744515 1103 15 0.8353489239651566 1103 16 0.9876054998723731 1103 17 1.0461819363876401 1103 18 0.9139398314650506 1103 19 0.8230644934918132 1103 20 0.841531390326395 1103 21 0.869962296566932 1103 22 0.9017399748896082 1103 23 0.9071809677492624 1103 24 0.9447997814417874 1103 25 0.9559773579432687 1104 1 0.3741754248123925 1104 2 0.4417731542366319 1104 3 0.5026255362317009 1104 4 0.5793089004516574 1104 5 0.6252064323258679 1104 6 0.5113149452967463 1104 7 0.523581584581521 1104 8 0.7843650748718076 1104 9 0.6117067165411072 1104 10 0.7584293629832786 1104 11 0.6487843979952359 1104 12 0.6006003234543655 1104 13 0.7061393237324651 1104 14 0.7287071907417584 1104 15 0.7413380674696768 1104 16 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-0.4506611364110569 2215 18 -0.5511959305201302 2215 19 -0.5341065946938954 2215 20 -0.3900071575127555 2215 21 -0.4722512885042036 2215 22 -0.5931417737045166 2215 23 -0.5140458067184355 2215 24 -0.42240890902645845 2215 25 -0.5338099706448034 2216 1 -0.620936195131609 2216 2 -0.49017852912874815 2216 3 -0.6824926479376927 2216 4 -0.4744044200062305 2216 5 -0.6383530153796002 2216 6 -0.8095266811693094 2216 7 -0.9259742057118601 2216 8 -1.0430420069012953 2216 9 -1.0567782897560463 2216 10 -1.051370624928424 2216 11 -1.059050820268773 2216 12 -1.249036746567782 2216 13 -1.2652028073496382 2216 14 -1.4517780840279997 2216 15 -1.2517194208074827 2216 16 -1.2854363654966008 2216 17 -1.2815743269133435 2216 18 -1.1969299623488112 2216 19 -1.2438056805491229 2216 20 -1.2470686567493345 2216 21 -1.4174435867855308 2216 22 -1.2817361293009668 2216 23 -1.228799481893624 2216 24 -1.2948660156701592 2216 25 -1.4651420626073772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37079.0 3 1.0 4 550.0 5 4866.0 6 6226.0 7 4800.0 8 3755.0 9 3252.0 10 2935.0 11 2589.0 12 2379.0 13 2188.0 14 2125.0 15 2088.0 16 2154.0 17 2231.0 18 2519.0 19 3098.0 20 4245.0 21 5778.0 22 7157.0 23 8272.0 24 9542.0 25 10565.0 26 11602.0 27 12701.0 28 14055.0 29 16954.0 30 21198.0 31 26969.0 32 34498.0 33 45191.0 34 59828.0 35 80000.0 36 115928.0 37 190466.0 38 712385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.215010514710386 19.349244914487162 13.200827565555306 26.234917005247148 2 13.98145588112417 21.02221585169596 40.02711851511185 24.969209752068025 3 16.8237447116077 26.358339016854927 30.004782309801293 26.813133961736085 4 10.906782579634124 17.288448984776572 37.98744547899349 33.81732295659582 5 12.124516697929321 39.000700945222256 35.50692072928217 13.367861627566253 6 30.974522084586457 38.02798371638369 18.07523674229514 12.922257456734714 7 26.81376073103544 32.4133329165721 22.699240180856254 18.073666171536203 8 25.49027733806547 35.98845591002784 20.65123016029382 17.87003659161287 9 26.68431514005334 14.220946930945422 20.164162050755323 38.93057587824592 10 14.411113375450038 29.326200665715646 34.577066775354936 21.685619183479382 11 33.512524716931274 22.971343643290954 24.2324760268692 19.283655612908575 12 22.801933745378417 25.978471221714354 31.707297192102263 19.512297840804962 13 28.730872610413616 22.22176937566271 25.97976183440896 23.067596179514716 14 21.27654371472414 21.936871793373896 28.26558760775928 28.520996884142686 15 23.301400858189513 30.02977239705496 24.19708606824352 22.471740676512002 16 21.7996031705599 28.565402477568814 27.389586385802172 22.245407966069113 17 20.912694260739116 28.477194193477377 28.197276646859553 22.412834898923947 18 21.03453243080536 27.512947453248515 30.060378414886546 21.39214170105958 19 22.613572218950406 26.680224892658384 29.293511818276297 21.41269107011491 20 23.511770727409694 26.63790638167221 29.06486959037991 20.785453300538183 21 23.229143670333627 26.37222303358002 28.136847003202785 22.261786292883563 22 22.795934697581657 27.212488265589613 28.45983401328383 21.531743023544898 23 21.877918900173697 27.251106762032407 28.654284699205956 22.216689638587937 24 22.37378996569008 26.725803898873025 29.134358894936657 21.766047240500242 25 22.20985948595471 26.868777026503803 28.685759622216516 22.235603865324972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 298.0 2 544.5 3 791.0 4 791.0 5 791.0 6 1946.0 7 3101.0 8 3101.0 9 3101.0 10 3579.5 11 4058.0 12 4058.0 13 4058.0 14 5584.0 15 7110.0 16 7110.0 17 7110.0 18 13270.0 19 19430.0 20 19430.0 21 19430.0 22 30190.5 23 40951.0 24 40951.0 25 40951.0 26 58264.5 27 75578.0 28 75578.0 29 75578.0 30 94078.0 31 112578.0 32 112578.0 33 112578.0 34 126613.5 35 140649.0 36 140649.0 37 140649.0 38 150672.0 39 160695.0 40 160695.0 41 160695.0 42 167879.5 43 175064.0 44 175064.0 45 175064.0 46 183367.5 47 191671.0 48 191671.0 49 191671.0 50 185671.5 51 179672.0 52 179672.0 53 179672.0 54 160165.0 55 140658.0 56 140658.0 57 140658.0 58 120500.5 59 100343.0 60 100343.0 61 100343.0 62 81455.0 63 62567.0 64 62567.0 65 62567.0 66 47763.0 67 32959.0 68 32959.0 69 32959.0 70 23860.0 71 14761.0 72 14761.0 73 14761.0 74 10302.0 75 5843.0 76 5843.0 77 5843.0 78 4032.5 79 2222.0 80 2222.0 81 2222.0 82 1535.5 83 849.0 84 849.0 85 849.0 86 554.0 87 259.0 88 259.0 89 259.0 90 158.0 91 57.0 92 57.0 93 57.0 94 31.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1901276280100994 2 0.0076078221997610326 3 0.00523037776233571 4 0.002037809517793134 5 0.08816922513651625 6 0.3513862878514627 7 0.03416727291499821 8 0.016777965029830134 9 0.005909647601600088 10 0.004686961890924208 11 0.0 12 0.0 13 0.0 14 0.0014943936463816315 15 0.0 16 0.0 17 0.020853584065416403 18 0.030635069750823442 19 0.0 20 0.0 21 9.509777749701291E-4 22 2.0378095177931335E-4 23 0.005570012681967899 24 0.0 25 2.0378095177931335E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1472169.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.5164167749769 #Duplication Level Percentage of deduplicated Percentage of total 1 85.93597870819107 64.89577184093298 2 10.27276785707711 15.515252378552427 3 2.135073760363527 4.8369935979878775 4 0.694200955159734 2.096942746217181 5 0.31137451879476374 1.1756943967206628 6 0.1710753347671175 0.7751397768115403 7 0.09945102308669765 0.5257129435679037 8 0.06857320946108102 0.4142722452208626 9 0.04719501560997533 0.32075986216539937 >10 0.2333018846256635 3.0794554331732407 >50 0.014788024882052931 0.791492513071503 >100 0.013426468109895883 1.9981087241089233 >500 0.0015317767035799203 0.802983689495355 >1k 9.911496317281838E-4 1.3768642451435056 >5k 2.7031353592586824E-4 1.3945556068305485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8919 0.6058407696398987 No Hit TATCAACGCAGAGTACTTTTTTTTT 6190 0.4204680305046499 No Hit GGTATCAACGCAGAGTACTTTTTTT 5386 0.36585473542779395 No Hit GTACATGGGAAGCAGTGGTATCAAC 2889 0.19624105656347876 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2855 0.1939315391099799 No Hit CCCATGTACTCTGCGTTGATACCAC 2570 0.17457234869094512 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2474 0.1680513582340071 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1824 0.12389881868182252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.792698392643779E-5 0.0 11 0.0 0.0 0.0 6.792698392643779E-5 0.0 12 0.0 0.0 0.0 6.792698392643779E-5 1.3585396785287558E-4 13 0.0 0.0 0.0 1.3585396785287558E-4 2.0378095177931338E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATCTG 100 1.4036414E-7 12.375719 5 CGTGCGC 55 0.0030996278 12.074526 10 GGCGAGG 390 0.0 11.676465 19 TCGAACT 75 2.0999499E-4 11.384553 19 GTCCTAC 720 0.0 11.377833 1 TGGCGAG 890 0.0 11.090979 18 CCATCGA 60 0.0059335013 11.068691 9 TCCTACA 820 0.0 11.032403 2 TAAGGCC 95 1.32894E-5 11.022909 4 CTAGGAC 235 0.0 10.939489 3 TTATACT 185 5.456968E-12 10.806242 4 TATGGCG 995 0.0 10.588665 16 TAGGACC 405 0.0 10.577538 4 GCGCCAC 100 2.4215442E-5 10.4408045 13 GACGTGA 505 0.0 10.336728 7 CATTATA 195 1.4551915E-11 10.255221 2 ATGGCGA 1010 0.0 10.243801 17 GTCTTAC 140 9.307951E-8 10.20603 1 GATCTAA 75 0.0025889042 10.16067 1 AGGACGT 845 0.0 10.139416 5 >>END_MODULE