FastQCFastQC Report
Thu 26 May 2016
SRR1513136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955525
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36960.1890029531711433No Hit
GTCCTAAAGTGTGTATTTCTCATTT34710.17749709157387403No Hit
CTGTAGGACGTGGAATATGGCAAGA30600.15647971772286215No Hit
CTTTAGGACGTGAAATATGGCGAGG30430.15561038595773513No Hit
GTACATGGGAAGCAGTGGTATCAAC26980.13796806484192226No Hit
GTCCTACAGTGTGCATTTCTCATTT25880.13234297694992395No Hit
CCCATGTACTCTGCGTTGATACCAC22280.11393359839429308No Hit
GTATCAACGCAGAGTACTTTTTTTT21190.10835964766494931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8700.013.0330624
CGTGCGC550.00309654912.07645310
GCACCGT550.003098922812.0752176
GGCGAGG9300.011.83711219
TCGAACT1451.0004442E-1011.78081219
CTCGAAC1604.7293724E-1111.26949118
CCAACGA1105.0375274E-711.21557119
AATGTCC6450.011.1795588
GACCGTG855.39233E-511.1625377
TGGCGAA1201.2942473E-711.07178118
TAGGACA7100.011.0046014
CCGTTTC803.8126306E-410.67499513
TAGAAAT7550.010.6011034
AAATGTC6550.010.5745877
ATTTAGA7500.010.5699851
TGGCGAG20500.010.55484118
GTCCTAC20950.010.5313851
TTAGGAC15700.010.4392623
TTTAGAA9050.010.4310742
TCCAACG1553.7271093E-910.40849518
Produced by FastQC (version 0.11.5)