##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513136_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1955525
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.566172766904028	31.0	30.0	34.0	25.0	34.0
2	29.40961583206556	31.0	30.0	34.0	23.0	34.0
3	29.615456207412333	31.0	30.0	34.0	24.0	34.0
4	32.6903506730929	37.0	33.0	37.0	24.0	37.0
5	32.86592705283747	37.0	35.0	37.0	25.0	37.0
6	33.21470091151993	37.0	35.0	37.0	27.0	37.0
7	33.18016082637655	37.0	35.0	37.0	27.0	37.0
8	33.07006149243809	37.0	35.0	37.0	26.0	37.0
9	34.31829099602408	39.0	35.0	39.0	25.0	39.0
10	33.900795949936715	39.0	34.0	39.0	22.0	39.0
11	34.339716955804704	39.0	35.0	39.0	27.0	39.0
12	34.340395545953136	39.0	35.0	39.0	27.0	39.0
13	34.380238043492156	39.0	35.0	39.0	27.0	39.0
14	34.62331445519745	40.0	34.0	41.0	17.0	41.0
15	35.06067884583424	40.0	35.0	41.0	24.0	41.0
16	34.90977001061096	40.0	34.0	41.0	21.0	41.0
17	34.53798238324747	39.0	34.0	41.0	17.0	41.0
18	34.27704273788369	39.0	33.0	41.0	17.0	41.0
19	34.90172715766866	40.0	34.0	41.0	22.0	41.0
20	35.120764500581686	40.0	35.0	41.0	25.0	41.0
21	35.16213548791245	40.0	36.0	41.0	25.0	41.0
22	34.85681492182406	40.0	35.0	41.0	18.0	41.0
23	34.393092903440255	40.0	34.0	41.0	16.0	41.0
24	34.6517145012209	40.0	35.0	41.0	18.0	41.0
25	34.288971503816114	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.116285372041446
1101	2	1.3129252518389016
1101	3	1.1762611016432913
1101	4	1.6983924687137275
1101	5	1.4195683019680914
1101	6	1.1764761875867435
1101	7	1.2194276381561693
1101	8	1.2991881635491325
1101	9	1.7090462957606078
1101	10	1.9696350691698115
1101	11	1.62852377853185
1101	12	1.5884039932299956
1101	13	1.4578670279669836
1101	14	2.3580106754199974
1101	15	2.0387669911613386
1101	16	2.004490522869979
1101	17	2.362827464857915
1101	18	2.444704438734732
1101	19	2.033184767219474
1101	20	1.6424952409980875
1101	21	1.7436305460629882
1101	22	1.9734786793285295
1101	23	2.2302311874231577
1101	24	1.9367995581069337
1101	25	2.2308902946160885
1102	1	0.9127513375971681
1102	2	1.0767110656879275
1102	3	0.9638103623278234
1102	4	1.2351973199939295
1102	5	1.191809819405563
1102	6	0.885376452934544
1102	7	0.7756086674092586
1102	8	0.8628690886080577
1102	9	1.2590131851712414
1102	10	1.482638963716056
1102	11	1.1315917177565566
1102	12	1.0551040020286138
1102	13	0.9977594995914387
1102	14	1.9454054972016692
1102	15	1.4696188826327017
1102	16	1.6007751905832208
1102	17	1.9050455501859886
1102	18	2.158813949114787
1102	19	1.5692672056407275
1102	20	1.3715408687794977
1102	21	1.162034013644707
1102	22	1.392617063068684
1102	23	1.7086954117786561
1102	24	1.5727599735869404
1102	25	1.9158752211939358
1103	1	0.6056160466759479
1103	2	0.7644551623154534
1103	3	0.746104371943801
1103	4	1.142777642314968
1103	5	0.9892197641721481
1103	6	0.6260610584248667
1103	7	0.6606131039664902
1103	8	0.609844663468941
1103	9	1.0334778749905453
1103	10	1.289269725221871
1103	11	0.9333051230377762
1103	12	0.8753677192445153
1103	13	0.8246065174683537
1103	14	1.6826285562938637
1103	15	1.368634242062619
1103	16	1.4238081449176363
1103	17	1.6849565439060257
1103	18	1.8906054928303462
1103	19	1.4299472713980705
1103	20	1.0104477561046963
1103	21	1.0582715379769922
1103	22	1.440236110687735
1103	23	1.8025028032893573
1103	24	1.466119582344497
1103	25	1.6897401159890606
1104	1	0.795754071400971
1104	2	1.1354814897453984
1104	3	0.9194019447551867
1104	4	1.3192141042777337
1104	5	1.147642993796815
1104	6	0.9150731914430281
1104	7	0.9010012543505752
1104	8	0.9315098159270221
1104	9	1.2965553048228387
1104	10	1.4762145247816036
1104	11	1.0973396991921192
1104	12	1.1571098510972107
1104	13	1.0777709434213065
1104	14	2.138812579660822
1104	15	1.6143451843683465
1104	16	1.6818038637347215
1104	17	2.089968304334377
1104	18	2.273134630658433
1104	19	1.611752424027145
1104	20	1.3288871717866826
1104	21	1.3908533918632884
1104	22	1.6803340190099405
1104	23	1.9431349819992718
1104	24	1.707822596767052
1104	25	1.9664760222620217
1105	1	0.5160851637189907
1105	2	0.7226221532049237
1105	3	0.6588241268062838
1105	4	1.1125215457000195
1105	5	1.017792876441355
1105	6	0.7539393473053266
1105	7	0.7969795601851999
1105	8	0.8624035154838481
1105	9	1.0433174238242273
1105	10	1.3619487910384862
1105	11	0.9801790947554281
1105	12	0.8474926598295625
1105	13	0.7772780900371643
1105	14	1.54736477943284
1105	15	1.2367833776701005
1105	16	1.3439069510252963
1105	17	1.6769525744378981
1105	18	1.4011934231302732
1105	19	1.17315093891672
1105	20	1.059192709232505
1105	21	0.9725593037739344
1105	22	1.1345616512431036
1105	23	1.469578757543701
1105	24	1.3381229907068644
1105	25	1.4838887683974704
1106	1	0.5039464028345151
1106	2	0.6051891191121754
1106	3	0.594297006265613
1106	4	1.0963012192034256
1106	5	0.9495503913625001
1106	6	0.6524005642194979
1106	7	0.5142785042760423
1106	8	0.6011423088435919
1106	9	0.783909049107713
1106	10	0.683284390952906
1106	11	0.595207294553056
1106	12	0.6487995853841468
1106	13	0.5485783540833538
1106	14	1.5121951457695033
1106	15	1.07953088225009
1106	16	1.339570742282305
1106	17	1.4994088797159932
1106	18	1.624753997715409
1106	19	1.085751904642521
1106	20	0.8723769179631446
1106	21	0.8124627422168231
1106	22	1.0536721111341123
1106	23	1.5204146759825008
1106	24	1.0726310703349782
1106	25	1.4207008943490322
1107	1	0.7692665693882113
1107	2	0.9661754547955255
1107	3	0.9776835093519942
1107	4	1.268004297533551
1107	5	1.174079710602264
1107	6	0.8824426537165309
1107	7	0.8620638081247591
1107	8	0.9699573987924666
1107	9	1.184527524985377
1107	10	1.4774073738734117
1107	11	0.9969222074943502
1107	12	1.0182910645631225
1107	13	0.9225081700093227
1107	14	1.820018806212424
1107	15	1.3948266193586747
1107	16	1.5316145654393054
1107	17	1.8844710634080357
1107	18	2.130968956369081
1107	19	1.5995089112615233
1107	20	1.416301566354612
1107	21	1.239711788665332
1107	22	1.5032221989777952
1107	23	1.7823025082485344
1107	24	1.5023566167134632
1107	25	1.8020120222830869
1108	1	0.7091783980561033
1108	2	0.927857723758855
1108	3	0.7932202111637032
1108	4	1.2676311227351604
1108	5	1.1688931107675202
1108	6	0.737490467345836
1108	7	0.7213024039364484
1108	8	0.7475945286359931
1108	9	1.0052240090861986
1108	10	1.1472225069062816
1108	11	0.7937086869529182
1108	12	0.7519282796172391
1108	13	0.7050981112453769
1108	14	1.6755523634506417
1108	15	1.191146170744389
1108	16	1.3029059935460623
1108	17	1.5915603140382188
1108	18	1.7755168790528018
1108	19	1.1599237051605726
1108	20	0.9991600507069549
1108	21	0.959939473953213
1108	22	1.2618879698519336
1108	23	1.5677051558539787
1108	24	1.2255599682146041
1108	25	1.5287553979438044
1109	1	0.4189716339348841
1109	2	0.502076828431381
1109	3	0.49141729551205415
1109	4	0.7372707521064967
1109	5	0.713098608893965
1109	6	0.34499345041846396
1109	7	0.31891311247495224
1109	8	0.3341532186984466
1109	9	0.6646137815741895
1109	10	0.8717266535865207
1109	11	0.4981465588710847
1109	12	0.3523549572052005
1109	13	0.3225661345741955
1109	14	1.274256489653446
1109	15	0.842776090721415
1109	16	0.9702188584613296
1109	17	1.4018864783380707
1109	18	1.452807985085336
1109	19	0.870520583704284
1109	20	0.6520273099312988
1109	21	0.6281042773666385
1109	22	0.9761134753322196
1109	23	1.260507917602908
1109	24	1.0239853952624642
1109	25	1.305503401233075
1110	1	0.5287539343230172
1110	2	0.7396422568319103
1110	3	0.6626990906546872
1110	4	0.9066091418839335
1110	5	0.832976791504997
1110	6	0.5638572369443153
1110	7	0.5651157930789665
1110	8	0.5654424964713627
1110	9	0.8167947142617962
1110	10	1.181272507896523
1110	11	0.8154140719315706
1110	12	0.6924539403946852
1110	13	0.5215898234815342
1110	14	1.3889072236083564
1110	15	1.1404842097741792
1110	16	1.1996949000827257
1110	17	1.5396021472052297
1110	18	1.1224404103204435
1110	19	0.7998278882336862
1110	20	0.707607989544762
1110	21	0.6541466230914494
1110	22	0.9157215736208926
1110	23	-0.42478417896333553
1110	24	0.27382756509087613
1110	25	0.8695466543632762
1111	1	0.1649846424946908
1111	2	0.43562788000207675
1111	3	0.43026594777743554
1111	4	0.7150286737572955
1111	5	0.7872677453412109
1111	6	0.35890161862782577
1111	7	0.32708404855850404
1111	8	0.36673964004406656
1111	9	0.6250039544693138
1111	10	0.8487041035983722
1111	11	0.46572562855560307
1111	12	0.3165166056552735
1111	13	0.2618581937314701
1111	14	1.2094276333873353
1111	15	0.7630731775339399
1111	16	0.8378073667977546
1111	17	1.2015479453562747
1111	18	1.4548165302115308
1111	19	1.0267808220197523
1111	20	0.6443912307892532
1111	21	0.3851523284947618
1111	22	0.8397002050071336
1111	23	1.2613869707173464
1111	24	0.8809254262631896
1111	25	1.0989252900960906
1112	1	0.06611763951932659
1112	2	0.28537256794988153
1112	3	0.16521708953803227
1112	4	0.5978568535794153
1112	5	0.2999309469447837
1112	6	0.1463875570788744
1112	7	0.004087055791366367
1112	8	0.09614073225064601
1112	9	0.29136150606550615
1112	10	0.5733019087751501
1112	11	0.13756853861373486
1112	12	0.1884575614900541
1112	13	0.016714752654010567
1112	14	0.625062057546323
1112	15	0.42523972363510865
1112	16	0.2788291673771113
1112	17	0.7924251204481862
1112	18	0.9000883974471421
1112	19	0.3802826921128144
1112	20	0.22608938788981447
1112	21	0.2433747362568468
1112	22	0.5208439732579322
1112	23	0.8005894538355207
1112	24	0.5305230986585769
1112	25	0.7859209996638938
1113	1	0.07679192904035759
1113	2	0.23261706445106967
1113	3	0.14193877071597072
1113	4	0.4843337679615445
1113	5	0.4623675300931467
1113	6	0.2970563314975365
1113	7	0.16454890466118144
1113	8	0.1789083307511916
1113	9	0.4084729029426484
1113	10	0.6390790604158951
1113	11	0.2056671661177205
1113	12	0.14981362141355703
1113	13	0.05864329112458222
1113	14	0.8107127440673594
1113	15	0.46691522265631136
1113	16	0.657166937364579
1113	17	0.7688674425793067
1113	18	0.7491557717942712
1113	19	0.4251130481099423
1113	20	0.30303915404401494
1113	21	0.21358739532796278
1113	22	0.4824399674370454
1113	23	0.5068558660430114
1113	24	0.39712271041721436
1113	25	0.7612117028940517
1114	1	-1.0971671717041716
1114	2	-0.7564572456535643
1114	3	-0.7430521348719061
1114	4	-0.4607845941280928
1114	5	-3.714550070491054
1114	6	-1.3852266758291911
1114	7	-1.0448017138935768
1114	8	-0.6607682045876686
1114	9	-0.5137377802295902
1114	10	-0.226019145761839
1114	11	-0.6853747166815793
1114	12	-0.7952534347185036
1114	13	-0.9231863159698719
1114	14	-6.25751588002219
1114	15	-2.5562952260821916
1114	16	-1.4849253432984142
1114	17	-0.48138006016248625
1114	18	-6.466581360623067
1114	19	-2.68500473463196
1114	20	-1.7372170523870167
1114	21	-1.3959685548427458
1114	22	-1.118724871814166
1114	23	-0.7635181231299271
1114	24	-0.7587747531175637
1114	25	-0.5532011214396348
1115	1	-0.8273274916661038
1115	2	-0.35419051989904915
1115	3	-0.4187012434487052
1115	4	-3.084109419715187
1115	5	-1.008589470745278
1115	6	-0.6686151626152679
1115	7	-0.5567782003813377
1115	8	-0.3236264169665759
1115	9	-0.17926080117187837
1115	10	-0.4499266523224179
1115	11	-0.5893938409281674
1115	12	-0.6108502435637675
1115	13	-0.6216867666277182
1115	14	-6.187039238726726
1115	15	-2.516640006556244
1115	16	-1.2065753488764202
1115	17	-0.3028000833415234
1115	18	-0.1381968400780167
1115	19	-0.447054824116357
1115	20	-0.4137400488805767
1115	21	-0.6638896261325655
1115	22	-1.8918369832001076
1115	23	-1.0221920282635182
1115	24	-0.6790204361194725
1115	25	-0.16546423330242277
1116	1	-0.7661193376037367
1116	2	-0.3359646560446876
1116	3	-0.4889851951161077
1116	4	-0.29686432938039786
1116	5	-0.8535004605156686
1116	6	-0.7283808968824843
1116	7	-0.7112491516082073
1116	8	-0.6210810125875597
1116	9	-0.5901299643652678
1116	10	-0.4579576107095491
1116	11	-0.7269514308662224
1116	12	-1.0063036906656606
1116	13	-0.9687020346528854
1116	14	-0.08832393815877282
1116	15	-0.5918269582960107
1116	16	-0.5863293355791583
1116	17	-0.16020026607201032
1116	18	-0.31397950674976727
1116	19	-0.5505468045839166
1116	20	-0.9928697981064687
1116	21	-1.10454813298157
1116	22	-1.1469588747611112
1116	23	-1.7819594129450493
1116	24	-1.41495583805672
1116	25	-0.9117847615365662
1201	1	0.7848605243596651
1201	2	0.8777688853849277
1201	3	0.8872969113369322
1201	4	1.2450152734114184
1201	5	1.0712084204201417
1201	6	0.8062840086914562
1201	7	0.7301958706053711
1201	8	0.8361486728938416
1201	9	0.9974070290862045
1201	10	1.4452658711894415
1201	11	0.7914385020807799
1201	12	0.8227516059743252
1201	13	0.7696624080807695
1201	14	1.6042143688835466
1201	15	1.475305778985593
1201	16	1.3860398514425185
1201	17	1.8061912355035048
1201	18	1.2558158780397335
1201	19	1.1426504439007132
1201	20	1.1981284563876997
1201	21	1.0315075750167821
1201	22	1.3638822593591087
1201	23	1.6439494326604702
1201	24	1.3423371889332216
1201	25	1.6039055897490258
1202	1	0.9939272104915382
1202	2	1.1129008128527005
1202	3	1.0910219301431603
1202	4	1.5684361078064413
1202	5	1.372134099150955
1202	6	0.9939586801991922
1202	7	1.000450218229183
1202	8	1.1331888575069087
1202	9	1.4092015927512875
1202	10	1.6875033877174062
1202	11	1.2197742111155847
1202	12	1.1136392982636494
1202	13	1.1369737921403384
1202	14	2.0393832892562003
1202	15	1.6626482578121085
1202	16	1.6856178349417021
1202	17	2.06775315164586
1202	18	2.214820665264071
1202	19	1.7528689115608742
1202	20	1.6024615826779112
1202	21	1.407243631316291
1202	22	1.6541231390422766
1202	23	1.85931212195905
1202	24	1.6495815126513307
1202	25	1.9611436165628007
1203	1	0.6138695094167943
1203	2	0.7214515167585738
1203	3	0.6770675214789073
1203	4	1.1022557202003185
1203	5	0.9921771120312002
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2211	19	-0.9227048757796155
2211	20	-1.1383252883465573
2211	21	-0.9991108604297594
2211	22	-0.8049957922164737
2211	23	-0.3152003623957711
2211	24	-0.9543155640403356
2211	25	-8.55810768796453
2212	1	-1.6412847430384971
2212	2	-4.188826684472151
2212	3	-1.9134387685310905
2212	4	-3.8368210641127156
2212	5	-1.6342955690543057
2212	6	-1.3222563032633268
2212	7	-1.2383633395868436
2212	8	-1.0974325430995862
2212	9	-1.3020394102194075
2212	10	-0.6666930116514749
2212	11	-1.2144166792851436
2212	12	-1.2709848415947533
2212	13	-1.4643184459266294
2212	14	-6.612220383219984
2212	15	-5.221713127113713
2212	16	-2.9813688483336733
2212	17	-7.517206180575805
2212	18	-3.144700646868902
2212	19	-2.1350432614393497
2212	20	-2.049566474979237
2212	21	-1.7152746356520296
2212	22	-1.7200081512621495
2212	23	-1.1667778350058384
2212	24	-1.6966518467318394
2212	25	-1.5146725633020495
2213	1	-0.2829437343247285
2213	2	-0.15538894989082408
2213	3	-0.17976080659776272
2213	4	0.20165107706927898
2213	5	-0.006902337212807197
2213	6	-0.21659685547279395
2213	7	-0.2905153394453919
2213	8	-0.1630072161123053
2213	9	0.001976782824044676
2213	10	-3.8077697188409374
2213	11	-1.3463383855824915
2213	12	-0.9354202229285491
2213	13	-0.7297162235222387
2213	14	0.03450556470959043
2213	15	-0.4610517825799505
2213	16	-0.18510137378298452
2213	17	0.21921442384231682
2213	18	0.31418342514947284
2213	19	-0.1902617967999447
2213	20	-0.6594777218628707
2213	21	-0.6819616762342946
2213	22	-0.8275755941468717
2213	23	-1.2053488318730032
2213	24	-0.8381581341141526
2213	25	-0.38293430261558825
2214	1	-0.9190033661729125
2214	2	-0.5874933885205742
2214	3	-0.5643175082727296
2214	4	-0.20555830236833117
2214	5	-3.5054829031603276
2214	6	-1.2980500243608155
2214	7	-1.164556936131497
2214	8	-0.7842177536831585
2214	9	-0.6937339193362817
2214	10	-4.36223201583724
2214	11	-1.640799769527689
2214	12	-1.1831685307805486
2214	13	-0.8326831590584263
2214	14	-0.10912097816656541
2214	15	-0.4926478559796479
2214	16	-0.4600381810354932
2214	17	-0.20139037640372237
2214	18	0.21385592813491172
2214	19	-0.6451774957525771
2214	20	-1.0213069596814464
2214	21	-0.9958437172399002
2214	22	-0.6334182802471133
2214	23	-0.36157367791576434
2214	24	-0.7316068723015547
2214	25	-0.5232002768050776
2215	1	-2.2910827174330244
2215	2	-4.774608377408057
2215	3	-2.9660021906667353
2215	4	-1.9204869327747325
2215	5	-1.9662764217512212
2215	6	-2.1954144362089387
2215	7	-2.205242740708698
2215	8	-2.306788251016659
2215	9	-8.149965629951382
2215	10	-3.60477632590122
2215	11	-2.952154244948222
2215	12	-3.258238004612295
2215	13	-2.614598669175596
2215	14	-7.765797309325315
2215	15	-4.490811093353887
2215	16	-9.134022248849263
2215	17	-4.321909018511139
2215	18	-2.733281366651866
2215	19	-3.1543018604252815
2215	20	-2.9714055962004053
2215	21	-3.1747278652267283
2215	22	-9.219770232477508
2215	23	-10.229452254519067
2215	24	-6.407383977428289
2215	25	-4.692678990214702
2216	1	-2.1703734746217194
2216	2	-2.0956264784266203
2216	3	-1.9671299109469444
2216	4	-7.3992012647530885
2216	5	-3.829699021441254
2216	6	-2.932618919242014
2216	7	-2.5491066900587143
2216	8	-7.771130839642744
2216	9	-4.134945980564925
2216	10	-2.526703230643559
2216	11	-2.5171757655057014
2216	12	-2.6546068038494433
2216	13	-2.4526057486832187
2216	14	-1.7711398684726163
2216	15	-2.39800989597169
2216	16	-2.0331850513909586
2216	17	-1.8000544726840673
2216	18	-7.4919685656050135
2216	19	-5.169862790457909
2216	20	-4.547709441760013
2216	21	-3.6268053981912196
2216	22	-3.21972873220394
2216	23	-2.404287157649904
2216	24	-2.9047515847608523
2216	25	-2.329402111888278
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85278.0
3	1.0
4	769.0
5	4280.0
6	5161.0
7	4630.0
8	4278.0
9	4111.0
10	3872.0
11	3620.0
12	3525.0
13	3609.0
14	3503.0
15	3619.0
16	3621.0
17	3890.0
18	4459.0
19	5541.0
20	7039.0
21	8912.0
22	11096.0
23	13534.0
24	15475.0
25	17489.0
26	20098.0
27	22219.0
28	25177.0
29	29870.0
30	36010.0
31	42858.0
32	54711.0
33	71431.0
34	95013.0
35	128128.0
36	184828.0
37	271243.0
38	752627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74843610091734	19.119831654178014	12.501613587327345	25.630118657577306
2	13.915105464464064	20.533526112324523	42.43957711672358	23.111791306487834
3	18.149754121937676	25.465542974952793	30.1592990411499	26.225403861959627
4	11.245074809477117	17.316689943237357	39.778229669126844	31.660005578158685
5	12.218559836428247	38.42177661770896	36.869065179016836	12.490598366845958
6	30.77759916201189	37.916404752759234	18.76768078708688	12.538315298141992
7	27.118035505222366	31.802793546681094	23.265643692629055	17.813527255467488
8	24.276532182624702	37.56621441989852	21.304510380262695	16.852743017214085
9	26.471948133251228	14.920692074935912	20.709720514493927	37.89763927731894
10	13.790616598946762	28.964836797073517	36.37950153239303	20.865045071586692
11	33.56702802648437	22.369937318265166	25.72922820396754	18.333806451282918
12	23.22062855505995	24.77170888406924	32.661128616442284	19.346533944428533
13	28.880792191842275	22.24144377873749	25.951658865226822	22.926105164193412
14	20.00120714754105	22.492176558626962	28.997883399777596	28.50873289405439
15	22.584092563593476	31.72626681761745	23.513125402551054	22.17651521623802
16	21.19514319632676	28.096227686072368	29.175091158413863	21.53353795918701
17	21.03147074953548	28.84370216667076	28.925197881623	21.199629202170765
18	20.894026726577415	27.408860161194287	31.09951977279749	20.597593339430805
19	23.11030132773669	27.17095460262785	27.946921223275318	21.771822846360145
20	23.467640207353117	27.645701167205306	28.330074897975678	20.5565837274659
21	22.602956959269427	26.981869671575154	27.36762895900751	23.04754441014791
22	22.880404698149327	28.65675658253532	27.35094201393401	21.111896705381344
23	21.57001258870536	28.209595550115168	28.47804547177465	21.742346389404823
24	22.84690757726452	27.393450473348807	28.540804590644353	21.21883735874232
25	22.274691196978115	27.50288263899333	28.442464367308062	21.779961796720492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	213.0
1	213.0
2	348.5
3	484.0
4	484.0
5	484.0
6	963.5
7	1443.0
8	1443.0
9	1443.0
10	2297.5
11	3152.0
12	3152.0
13	3152.0
14	5890.5
15	8629.0
16	8629.0
17	8629.0
18	16297.0
19	23965.0
20	23965.0
21	23965.0
22	39324.0
23	54683.0
24	54683.0
25	54683.0
26	79315.0
27	103947.0
28	103947.0
29	103947.0
30	133397.0
31	162847.0
32	162847.0
33	162847.0
34	183879.5
35	204912.0
36	204912.0
37	204912.0
38	219356.5
39	233801.0
40	233801.0
41	233801.0
42	241633.0
43	249465.0
44	249465.0
45	249465.0
46	254165.5
47	258866.0
48	258866.0
49	258866.0
50	243196.5
51	227527.0
52	227527.0
53	227527.0
54	197975.0
55	168423.0
56	168423.0
57	168423.0
58	142192.5
59	115962.0
60	115962.0
61	115962.0
62	93445.5
63	70929.0
64	70929.0
65	70929.0
66	54477.5
67	38026.0
68	38026.0
69	38026.0
70	27681.5
71	17337.0
72	17337.0
73	17337.0
74	12186.0
75	7035.0
76	7035.0
77	7035.0
78	4813.0
79	2591.0
80	2591.0
81	2591.0
82	1747.5
83	904.0
84	904.0
85	904.0
86	595.5
87	287.0
88	287.0
89	287.0
90	180.5
91	74.0
92	74.0
93	74.0
94	45.0
95	16.0
96	16.0
97	16.0
98	11.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17146290638063946
2	0.2610552153513762
3	0.2642768565986116
4	0.44213190831106736
5	0.25906086600784956
6	0.01871620153155802
7	0.019227573158103323
8	0.016568440700067757
9	0.018460515718285372
10	0.02111964817632094
11	0.020966236688357346
12	0.028892496899809512
13	0.027818616484064383
14	0.025721992815228648
15	0.04111427877424221
16	0.021068511013666406
17	0.04198361053936922
18	0.03155162935784508
19	0.027818616484064383
20	0.030631160430063538
21	0.034722133442425945
22	0.04515411462395009
23	0.030580023267409006
24	0.04812007005791284
25	0.03676761994860715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1955525.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.36052932241294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29281644311597	63.42418978109511
2	10.427311128553335	15.507607498574263
3	2.324623439664828	5.185806883462947
4	0.8165966790273242	2.4289024518158553
5	0.34840531534821634	1.2953801834017789
6	0.19022030365918874	0.8486929480780439
7	0.11551709228228654	0.6012938489527797
8	0.0832604800665363	0.4953034695508681
9	0.06678816910940472	0.44697632467051474
>10	0.29772330813342857	3.829639552055376
>50	0.019254780732769927	0.9885003968312103
>100	0.014071088390338235	2.104648558312154
>500	0.002297723943651818	1.0974387564496824
>1k	0.0011140479726796599	1.7456193467494237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3696	0.1890029531711433	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3471	0.17749709157387403	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3060	0.15647971772286215	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3043	0.15561038595773513	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2698	0.13796806484192226	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2588	0.13234297694992395	No Hit
CCCATGTACTCTGCGTTGATACCAC	2228	0.11393359839429308	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2119	0.10835964766494931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	870	0.0	13.033062	4
CGTGCGC	55	0.003096549	12.076453	10
GCACCGT	55	0.0030989228	12.075217	6
GGCGAGG	930	0.0	11.837112	19
TCGAACT	145	1.0004442E-10	11.780812	19
CTCGAAC	160	4.7293724E-11	11.269491	18
CCAACGA	110	5.0375274E-7	11.215571	19
AATGTCC	645	0.0	11.179558	8
GACCGTG	85	5.39233E-5	11.162537	7
TGGCGAA	120	1.2942473E-7	11.071781	18
TAGGACA	710	0.0	11.004601	4
CCGTTTC	80	3.8126306E-4	10.674995	13
TAGAAAT	755	0.0	10.601103	4
AAATGTC	655	0.0	10.574587	7
ATTTAGA	750	0.0	10.569985	1
TGGCGAG	2050	0.0	10.554841	18
GTCCTAC	2095	0.0	10.531385	1
TTAGGAC	1570	0.0	10.439262	3
TTTAGAA	905	0.0	10.431074	2
TCCAACG	155	3.7271093E-9	10.408495	18
>>END_MODULE