FastQCFastQC Report
Thu 26 May 2016
SRR1513136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955525
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37530.1919177714424515No Hit
GTCCTAAAGTGTGTATTTCTCATTT36220.1852188031347081No Hit
CTTTAGGACGTGAAATATGGCGAGG35500.1815369274235819No Hit
CTGTAGGACGTGGAATATGGCAAGA34510.17647434832078343No Hit
GTATCAACGCAGAGTACTTTTTTTT30510.1560194832589714No Hit
GTACATGGGAAGCAGTGGTATCAAC29620.15146827578271818No Hit
CCCATGTACTCTGCGTTGATACCAC28490.1456897764027563No Hit
GTCCTACAGTGTGCATTTCTCATTT25780.13183160532337862No Hit
TATCAACGCAGAGTACTTTTTTTTT22250.1137801869063295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG551.9248082E-413.848195
TCCAACG957.483504E-812.98757418
TAGGACC7350.012.1760454
CCTACAC2700.011.2843
GGCGAGG11050.011.2476519
TGGCGAG22000.010.61257418
GATATAC4400.010.3914761
GTAGGAC21000.010.336953
TGTAGGA22200.010.2944232
GGACGTG20850.010.2273496
AGGACCT13600.010.2206775
ACTGTTC5050.010.1466298
AGGACGT21350.010.0780455
TTAGGAC14800.010.0355173
GACGTGA11750.010.0149247
CCTATAC2002.3646862E-119.9969193
TGGCAAG16900.09.99635318
CCAACGA1054.165399E-59.93930819
TAGGACA7000.09.9286584
CTGTAGG22350.09.8451671
Produced by FastQC (version 0.11.5)